QIAsymphony RNA Kit

从组织或细胞中自动纯化总RNA，或是含有miRNA的总RNA

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QIAsymphony RNA Kit

Cat. No. / ID: 931636

For as many as 192 preps, depending on protocol: includes 2 reagent cartridges and enzyme racks and accessories

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QIAsymphony RNA Kit 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的（再）订购

特点

预装试剂条，使用方便
标准化的操作确保高重复性
经优化的操作得到高产量、高纯度的RNA
条形码实现样本和试剂的追踪

产品详情

QIAsymphony RNA Kit可在QIAsymphony SP核酸提取纯化分析仪上从1–96个样本中自动纯化总RNA，或是含有miRNA的总RNA和其他小分子RNA。可从动物细胞、组织或者福尔马林固定、石蜡包埋组织中纯化总RNA，需要的起始材料量少（≥100个细胞），至多1 x 10⁷个细胞或50 mg的组织。经优化的实验方案可获得高产量、高纯度的总RNA，或是含有miRNA的总RNA；洗脱体积灵活，因而可以根据不同下游应用优化调整RNA浓度。预装试剂条减少了工作站设置时间，并减少了手动操作。条形码阅读器进行试剂全程跟踪。

绩效

在QIAsymphony SP工作站上的RNA自动纯化流程与手动纯化流程相同，从培养细胞、组织和福尔马林固定、石蜡包埋组织中，纯化得到高产量、高品质RNA（参见"Reproducible purification of RNA"）。QIAsymphony纯化总RNA的实验方案主要纯化大于200个核苷酸的RNA，包括mRNA（参见"Highly pure, intact RNA"）。QIAsymphony miRNA实验方案也纯化总RNA，但不排除<200个核苷酸的RNA，如 miRNA和其他小分子RNA（参见"Automated purification comparable to manual procedure"）。

原理

QIAsymphony RNA Kit将硅胶膜技术的速度、效率与易于操作的磁性颗粒相结合（参见"Efficient handling of magnetic particles"）。在蛋白酶K处理后，纯化>200个核苷酸的总RNA，或包含miRNA的总RNA和其他小分子。起始材料包括动物细胞和组织。配合一些额外的组分，可以用该试剂盒纯化福尔马林固定、石蜡包埋组织。裂解样本，然后通过将其结合到离液盐中的硅胶表面上的颗粒中，将RNA从裂解物中一步纯化。使用磁珠将颗粒从裂解物分离，然后使用不含RNase的DNase处理，去除DNA。充分洗涤磁珠，随后用不含RNase的纯水洗脱得到RNA。

程序

创新的即用型试剂条预装有磁性颗粒和所有纯化过程需要的试剂，包括附加的酶。这些试剂条可在QIAsymphony SP工作站上被自动打开，有助于降低污染的风险（参见"Prefilled, sealed reagent cartridges"）。台面设置快速，节省你的宝贵时间。只需简单的在试剂置入模块中加载至多2个试剂条。试剂条可以来自相同的试剂盒，也可以是不同的试剂盒，因此可在处理96个样本的同一批次中，运行不同的纯化步骤。试剂仅会用于所选数目的样本，实现成本控制。纯化操作流程包括4 个步骤：裂解、结合、洗涤和洗脱。提供多种在QIAsymphony SP工作站上进行RNA纯化的实验方案，取决于起始材料和所需的RNA产物（参见下表）。

使用QIAsymphony SP纯化RNA的实验方案
实验方案	配套使用	样本材料（预处理样本裂解物）	处理体积（μl）
RNA CT 400	QIAsymphony RNA Kit (192)	≤3 x 10⁶个培养细胞 ≤20 mg易裂解的组织	400 μl
RNA CT 800	QIAsymphony RNA Kit (2 x 48)	3 x 10⁶–1 x 10⁷个培养细胞 ≤50 mg易裂解的组织	800 μl
RNA FT 400^*	QIAsymphony RNA Kit (192)	≤20 mg纤维组织	400 μl
miRNA CT 400^*	QIAsymphony RNA Kit (192)	≤3 x 10⁶个培养细胞 ≤20 mg组织	400 μl
RNA FFPE 130^†	QIAsymphony RNA Kit (2 x 72)	1–2 x FFPE切片至多10 μm 1 x FFPE切片20 μm	130 μl

应用

使用QIAsymphony RNA Kit在QIAsymphony SP核酸提取纯化分析仪上实现总RNA的全自动纯化过程，得到高品质RNA，广泛用于各种下游应用。

辅助数据和图表

Reproducible purification of RNA.

RNA was purified from Jurkat cells on the QIAsymphony SP using the QIAsymphony RNA Kit. RNA yields from 2 instrument runs using 2 different reagent cartridge lots were compared with manual purification using the RNeasy Mini Kit. Each bar represents the average of 4 samples.

Automated purification comparable to manual procedure.

Efficient handling of magnetic particles.

Specifications

Features	Specifications
ApplicationsZH	RT-PCR; Real-Time PCR; Expression Profiling
Sample amount	400 µl lysate from <20 mg tissue or 3 x 10e6 cells or 800 µl lysate from <50 mg tissue or 3 x 10e6 cells
Elution volume	50-200 µl

资源

Download Safety Data Sheets for QIAGEN product components.

For use with software version 4.0

For use with software version 4.0 or higher

For use with software version 4.0

For use with the QIAsymphony SP/AS instruments (software version 4.0)

FAQ

RNA processed on the QIAsymphony SP with the QIAsymphony RNA Kit can be eluted in 200, 100, or 50 µl.

Elution in smaller volumes will provide higher concentration, but lower overall yield. Total RNA yield with elution in 50 µl will be about 20% lower compared to elution in 100 µl.

FAQ ID -1943

Using the Standard protocol for RNA extraction on the QIAsymphony SP, up to 400 µl of lysate can be processed while the Large-Volume protocol allows up to 800 µl of homogenate.

FAQ ID -1939

No. Modified QIAGEN protocols or custom protocols for QIAsymphony SP can be developed to meet your specific requirements and purchased from QIAGEN.

FAQ ID -1904

Yes. Any number of samples between 1 and 96 can be processed on the QIAsymphony SP.

FAQ ID -1906

For purification of RNA from blood on the QIAsymphony SP, we recommend using the QIAsymphony PAXgene RNA Kit, since it includes stabilization of gene expression profiles.

FAQ ID -1945

For purification of RNA on the QIAsymphony SP, tissue samples require mechanical disruption and homogenization in the supplied lysis buffer (Buffer RLT Plus), e.g., by using the TissueLyser or TissueRuptor. Cultured cells should be lysed by vortexing in the lysis buffer contained in the kit.

FAQ ID -1938

200–1000 µl sample volumes can be processed on the QIAsymphony Sample Prep Module.

FAQ ID -1907

Yes, the QIAsymphony RNA Kit is compatible with stabilized samples. We recommend stabilizing tissue samples with RNAprotect Tissue Reagent or AllProtect Tissue Reagent, or by flash-freezing. Cultured cells can be lysed directly in cell culture dishes, or after pelleting. Alternatively, cells can be stabilized using RNAprotect Cell Reagent.

FAQ ID -1944

The QIAsymphony RNA Kit enables purification of RNA from cultured cells and easy-to-lyse tissues, such as kidney, liver, and spleen on the QIAsymphony SP. A large-volume protocol is available that enables processing of larger sample amounts.

Cultured cells: up to 3x10⁶ cells (standard protocol); up to 1x10⁷ (large volume protocol)
Tissues: up to 20 mg of most tissues (standard protocol); up to 50 mg (large-volume protocol)

For particularly DNA-rich tissues, such as spleen or thymus, we recommend either using no more than half the maximum amount, or using DNase at a higher concentration (see the QIAsymphony RNA Handbook; additional DNase required).

FAQ ID -1937

Strongly denaturing conditions in the lysis buffer (Buffer RLT Plus) of the QIAsymphony RNA Kit keep RNases in an inactive state. Keeping homogenates on the QIAsymphony instrument at room temperature (20-25°C) for over 6 hours does not impair RNA integrity.

FAQ ID -1941

No. Protocols on the QIAsymphony SP cannot be modified by users. However, protocols allow elution volumes to be selected within a predefined range. Customized protocols for QIAsymphony SP will be available on request.

FAQ ID -1903

Depending on the application, elution volumes on the QIAsymphony SP range from 30 µl to 500 µl.

FAQ ID -1908

Please see expected yields of total RNA isolated with the QIAsymphony RNA Kit on the QIAsymphony SP in the table below:

Sample type	Sample amount	Typical RNA yield (µg)*
Cultured Cells
HeLa, Jurkat	1 x 10e6 cells	15
Rat tissues
Kidney	10 mg	10-25
Liver	10 mg	20-50
Lung	10 mg	5-10
Spleen	10 mg	25-35

*RNA yields strongly depend on the type and quality of the sample material.

FAQ ID -1942

Each QIAsymphony RNA Kit enables preparation of up to 192 samples (96 per reagent cartridge) using the standard protocol on the QIAsymphony SP. If only the large-volume protocol is used, the number of samples that can be prepared per kit is reduced to 96 (48 per reagent cartridge). Any combination of standard and large-volume sample preparation can be performed.

FAQ ID -1940