TransMessenger Transfection Reagent

高效的mRNA转染

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TransMessenger Transfection Reagent (0.5 ml)

Cat. No. / ID: 301525

For 60 transfections in 6-well plates or 80 transfections in 12-well plates

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TransMessenger Transfection Reagent 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

高效的转染
严格的质量监控确保可靠的结果
高效转染原代细胞

Product Details

TransMessenger Transfection Reagent是即用型、脂质体试剂，用于真核细胞的RNA转染。使用RNA而非DNA转染的细胞为转染实验提供了新的可能性。使用TransMessenger Reagent成功转染的细胞株包含293T、Jurkat细胞和Vero细胞。该产品也可用于RNA转染人类原代成纤维细胞、大鼠心肌细胞和大鼠神经细胞。

Performance

TransMessenger Reagent提供快速简单的操作流程和高转染效率（参见""）。因为RNA量是成功转染的关键因素，我们推荐对于每个细胞类型和RNA，都优化RNA和TransMessenger Transfection Reagent量（参见""）。为了操作便利，该试剂配套提供优化准则和在不同细胞培养物规格下的优化起始点。

See figures

TransMessenger Reagent with HeLa cells.

Amount of RNA and TransMessenger Reagent vs. transfection efficiency.

Principle

TransMessenger Transfection Reagent是首款专为用RNA转染细胞的试剂。该试剂由脂质体制备，与特定的RNA冷凝增强剂和优化的缓冲液一起使用。RNA分子通过增强剂冷凝，然后涂上TransMessenger Reagent有效地转移到真核细胞中。严格的质量监控用于测试无RNA酶活性、每批次的一致性和低内毒素水平（≤10 EU/ml）。我们严格的标准消除了试剂的变量，避免对RNA转染效率产生不利影响。

Procedure

TransMessenger Reagent为即用型试剂。为获得TransMessenger–RNA转染混合物，只需简单混合具增强剂R的RNA和Buffer EC-R，并在室温下孵育5分钟，然后加入TransMessenger Reagent再孵育5–10分钟。将其与培养液混合并直接加入细胞中。随后需要3小时的孵育，培养液发生改变，孵育细胞直到可用于分析应用。使用高纯度的RNA可获得最佳的转染结果，无DNA、蛋白质和其他污染物。推荐使用RNeasy和Oligotex mRNA Kits纯化RNA。

Applications

RNA而非DNA转染的细胞为转染实验提供新的可能性。转染RNA序列的表达无需转录、不依赖于启动子。此外，蛋白表达通常会在RNA而非DNA转染后快速出现。使用TransMessenger Transfection Reagent的RNA转染可用于：

研究无法用质粒DNA高效转染的细胞
直接研究RNA功能

Supporting data and figures

Amount of RNA and TransMessenger Reagent vs. transfection efficiency.

Optimization experiments were performed in CHO-K1 cells. Cells (2 x 10⁴⁾ were seeded in quadruplicate into 96-well plates and transfected 24 hours later with an in vitro-transcribed CAT-encoding RNA using [A] increasing amounts of RNA with 1.5 µl TransMessenger Reagent and [B] increasing amounts of TransMessenger Reagent with 0.25 µg RNA, as described in the TransMessenger Transfection Reagent Handbook. CAT activity was measured 24 hours post-transfection.

Specifications

Features	Specifications
Applications	Direct studies of RNA function
Features	Transfection with RNA. Efficient transfection of neuronal cells. Tested for the absence of RNases.
Controls	Not included
Nucleic acid	RNA
Cell type	Eukaryotic cells
Number of possible transfections	80 transfections in 12-well plates / 0,5 ml reagent
Technology	Lipid-based formulation in conjunction with a RNA-condensing enhancer
Transfection type	Transient transfection, co-transfection

Resources

Search for transfection data by nucleic acid, cell line, and transfection reagent. Our database contains data from researchers like yourself who have shared their experimental results with us.

Transfection protocols for specific cell types and plate formats that save you the time and effort of adapting existing protocols to fit your requirements. Simply select the cell type, nucleic acid, and culture format to receive a QIAGEN transfection protocol to print out or download in convenient PDF format.

For transfection of eukaryotic cells with RNA and siRNA

Brochure detailing reagents for efficient and robust DNA and RNA transfection.

Download Safety Data Sheets for QIAGEN product components.

The following procedure is for co-transfection of adherent cells using siRNA and plasmid DNA in one well of a 24-well plate. This procedure is provided as a starting point for optimization of siRNA and plasmid DNA co-transfection in mammalian cells using TransMessenger™ Transfection Reagent.

Brochure detailing reagents for efficient and robust DNA and RNA transfection.

For transfection of eukaryotic cells with RNA and siRNA

Download Safety Data Sheets for QIAGEN product components.

Search for transfection data by nucleic acid, cell line, and transfection reagent. Our database contains data from researchers like yourself who have shared their experimental results with us.

FAQ

QIAGEN Transfection Reagents have been used successfully with many different cell types. For your convenience, we have organized data from researchers who have shared their experimental results with us online in our Transfection Cell Database. Simply type your cell line of interest into the 'Search' field on this page, and find transfection results achieved with various QIAGEN Transfection Reagents. Please note that QIAGEN cannot verify data supplied from outside sources.

You can also submit your own transfection data and obtain a gift as appreciation. In addition you can find on our TransFect Protocol Database transfection protocols for specific cell types and plate formats.

FAQ ID -158

Yes, please follow the Supplementary Protocol 'Guidelines for co-transfection of adherent cells with siRNA and plasmid DNA using TransMessenger Transfection Reagent' (TFP21).

In addition, we have also the following Supplementary Protocol for the Attractene Transfection Reagent 'Fast-Forward cotransfection of adherent cells with siRNA and DNA in 24-well plates using Attractene Transfection Reagent'.

FAQ ID -969

Unfortunately, we do not have any data for the transfection of siRNA into insect cells. However, we know about customers who have successfully used Effectene Transfection Reagent for the transfection of plasmid DNA into insect cell lines S2 and Sf9 (see FAQ 397). Since Effectene Transfection Reagent and HiPerFect Transfection Reagent for the transfection of eukaryotic cells with siRNA are both lipid based, there is a chance that HiPerFect will work with insect cells. It is especially suitable for transfection of very low siRNA concentrations, reducing the risk of cell toxicity and off-target knockdown effects.

Alternatively, you can try RNAifect or TransMessenger Transfection Reagent, also lipid-based, for the transfection of siRNA into S2 cells.

We would appreciate any feedback on your results in case you want to give it a try. Please visit our Transfection Cell Survey page to submit your feedback.

FAQ ID -1046

The composition of 1x PBS solution is:

137 mM NaCl
2.7 mM KCl
4.3 mM Na₂HPO₄
1.47 mM KH₂PO₄

Adjust to a final pH of 7.4.

This solution is not supplied in any QIAGEN Kit, but is used in protocols for various QIAGEN transfection kits.

FAQ ID -1030

No, we do not recommend to use RNAiFect for the transfection of plasmid DNA. The RNAiFect Kit does not contain Enhancer which is necessary to form condensed complexes before transfection of DNA into the cell. TransMessenger Transfection Reagent would be the right choice as it allows co-transfection of siRNA and plasmid DNA. Access the protocol via the following link: Co-transfection of adherent cells with siRNA and plasmid DNA using TransMessenger Transfection Reagent

Alternatively, you can use the Attractene Transfection Reagent with the QIAGEN Protocol "Fast -Forward cotransfection of adhernet cells with siRNA and DNA in 24-well plates using Attractene Transfection Reagent".

FAQ ID -515