QIAxcel DNA Kits

使用QIAxcel体系全自动分析DNA片段

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QIAxcel DNA High Resolution Kit (1200)

Cat. No. / ID: 929002

QIAxcel DNA High Resolution Gel Cartridge, Buffers, Mineral Oil, QX Intensity Calibration Marker, 12-Tube Strips

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Cartridge type

High Resolution

Screening

Fast Analysis

High Sensitivity

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

采用安全、操作简单的即用型预制胶
简化的工作流程，分析时间最短化
可检测到低至0.1 ng/µl的核酸，结果可靠
分辨率可达3–5 bp
标准化的处理过程，包括数字化数据采集

Product Details

配合QIAxcel体系，QIAxcel DNA Kits每批可全自动依据大小分离和定量多达96个样本DNA片段。QIAxcel技术基于使用凝胶卡夹的毛细管电泳，实现无可比拟的高分辨率、高速和高通量。QIAxcel凝胶卡夹可重复使用，每批12个样本可使用250次。仪器预装的处理方法适合大多数应用。QIAGEN也可提供用户需要的处理方法。

Performance

简单的高分辨率分析

生物基因分型、基于PCR的cDNA文库筛选以及许多生物医药研究和分子生物学应用都要求对PCR产物片段的大小有很高的分辨率。QIAxcel High Resolution Kit配合QIAxcel仪，可对15 bp–5 kb的DNA片段进行分析。分离小于500 bp的片段，分辨率达3–5 bp，其数据可靠性比传统的高分辨率琼脂糖凝胶电泳更高（参见" Sensitive detection of a mutated cancer-related gene"）。QIAxcel体系对于未经稀释的扩增反应中的DNA检测灵敏度达0.1 ng/µl。QIAxcel体系利用少量样本也可获得可靠的结果。

QIAxcel DNA Screening Kit非常适合限制性内切酶酶切产物、单重或多重定量PCR产物和其他应用产物DNA片段的常规分析（参见" High-resolution analysis of multiplex PCR samples"）。使用凝胶卡夹使工作流程更加高效，减少对大小在15 bp–3 kb之间的DNA片段进行分析的时间。处理96个样本在大约1小时内即可完成。对于小于1 kb的片段，其分辨率为20–50 bp。每次分析样本消耗量少于0.1 µl，为其他下游应用节约了珍惜样本。

QIAxcel DNA Fast Analysis Kit非常适合在单重或多重定量PCR产物应用中对DNA片段进行常规分析。使用凝胶卡夹使工作流程更加高效，减少对大小在15 bp–3 kb之间的DNA片段进行分析的时间。处理12个样本在大约3分钟内即可完成。处理96个样本在大约25分钟内即可完成（参见" Screenshot showing data from PCR fragment analysis"）。使用QIAxcel DNA Fast Analysis凝胶卡夹分辨率可达50 bp，分析样本消耗量少于0.1 µl。

See figures

Sensitive detection of a mutated cancer-related gene.

High-resolution analysis of multiplex PCR samples.

Screenshot showing data from PCR fragment analysis.

Principle

QIAxcel体系采用毛细管凝胶电泳技术，能够依据核酸的大小进行快速分离（参见" Sample separation process"）。不同于传统的琼脂糖凝胶电泳，分离是在含有即用型预制胶的毛细管中进行。样本自动上样到单个的毛细管中，然后加压。带负电的核酸通过毛细管移到正极。使用琼脂糖凝胶电泳时，小分子核酸比大分子核酸移动得快。而毛细管电泳，它们会通过一个检测和衡量荧光信号的检测器。光电倍增管器将荧光信号转为电子数据，然后传输到电脑上再使用QIAxcel ScreenGel软件进一步处理。处理后，可通过电泳图谱和胶图两种方式查看数据。

See figures

Sample separation process.

Procedure

QIAxcel DNA Kits简化了分离和分析DNA的流程，且更加安全。只需简单的几个步骤即可：预制胶卡夹、预装缓冲液槽及装载有样本的96孔板或PCR管或联管、选择分离方法。无需冗长的制胶及用户培训，简化了日常工作的整个流程。运行开始几分钟内，会在电脑屏幕上实时出现第一个结果（参见" Reduced run times"）。

See figures

Reduced run times.

Applications

预设方法配合相应的凝胶卡夹，能够分离和分析多种核酸，包括单重或多重PCR片段、限制性内切酶消化的DNA和合成的寡聚核苷酸。

	QIAxcel DNA High Resolution Kit	QIAxcel DNA High Sensitivity Kit^§	QIAxcel DNA Screening Kit	QIAxcel DNA Fast Analysis Kit
Size range	15 bp – 20 kb; displaying up to 50 kb	15 bp – 3 kb	15 bp – 5 kb	15 bp – 3 kb
Resolution*	3–5 bp	20–50 bp	20–50 bp	50 bp
Limit of detection	0.1 ng/μl	5 pg/μl	0.1 ng/μl	0.1 ng/μl
Samples per cartridge	1200 samples	1200 samples	2400 samples	3000 samples
Run time^†	9–25 min	~9 min	~7 min	~3 min
Application area^‡	High-resolution genotyping VNTR, STRs, etc. gDNA integrity check NGS library check	Quality control of liquid biopsy extracts NGS library check	Fast PCR screening (single and multiplex) Restriction digest, plasmid gDNA integrity check NGS library check	Fast single amplicon analysis, e.g., HLA typing

STR: short tandem repeat; VNTR: variable number of tandem repeat.

* For 100–500 bp range.

^† For 12 samples.

^‡ Including, but not limited to.

^§ Only works in combination with the QIAxcel Connect System.

Supporting data and figures

Reduced run times.

Nucleic acid separation on the QIAxcel system using the QIAxcel DNA High Resolution Kit takes less than 10% of the time of conventional high-resolution agarose.

Resources

A protocol for evaluation of samples for next-generation sequencing on the QIAGEN GeneReader platform using the QIAxcel Advanced Instrument and ScreenGel software version 1.5 of higher

For evaluation of samples for next-generation sequencing (NGS) on the QIAGEN GeneReader platform using ScreenGel Software version 1.5 or higher

For evaluation of samples for next-generation sequencing (NGS) on the QIAGEN GeneReader instrument

For analysis and typing of PCR products from different HLA loci using the QIAxcel Advanced and the Helmberg-SCORE software

For automated analysis of DNA fragments using the QIAxcel and QIAxcel Advanced instruments

The QIAxcel System was successfully used to detect DNA derived from genetically modified organisms (GMOs) at a level suitable for GMO testing according to EU standards.

Download Safety Data Sheets for QIAGEN product components.

For electrophoretic analysis of DNA samples using the QIAxcel Connect system

This release includes the first version of QIAxcel ScreenGel NGS Profiles. These profiles are intended to be used to run and analyze reactions of GeneRead QIAact AIT and BRCA 1/2 panels using the QIAxcel instrument and QIAxcel ScreenGel 1.5 Software. These profiles are composed of an Process Profile, Analysis Profile, Distribution Profile, and Report/Export Profile.

For use with QIAxcel Advanced instruments and QIAxcel ScreenGel Software version 1.6