EZ1 RNA Card

预设程序卡，配合EZ1 RNA Kit在BioRobot EZ1工作站上进行RNA纯化

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EZ1 RNA Card

Cat. No. / ID: 9015590

Pre-programmed card for EZ1 RNA purification protocols

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EZ1 Cards配合EZ1 Kits在BioRobot EZ1工作站上使用。请参照EZ1 Kit操作手册和BioRobot EZ1用户手册，获得更多产品使用信息。

EZ1 RNA Card 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的（再）订购

特点

程序设置简单，只需插卡就可以运行
标准化操作流程，确保高重复性结果
预设程序，提高对细胞和组织样本的处理能力
经优化的操作流程，确保可靠纯化

产品详情

EZ1 RNA Card预装有纯化程序，配合EZ1 RNA Kit从细胞和容易裂解的人类或动物组织中纯化总RNA或总核酸，或从任何类型的人类或动物组织中纯化总RNA的程序。这些程序在控制面板提供操作向导，并提供BioRobot EZ1工作站的运行指令。

绩效

使用EZ1 RNA Kits和BioRobot EZ1工作站从细胞和组织中纯化获得高品质RNA和核酸适用于各种下游应用。

原理

EZ1 RNA Card预装有纯化程序，用于从细胞和容易裂解的人类或动物组织中纯化总RNA或总核酸。EZ1 RNA Card配合EZ1 RNA Kits在BioRobot EZ1工作站上使用。

程序

只需在卡槽中插入预装有程序的EZ1 RNA Card并运行工作站即可。

应用

应用EZ1 RNA Card，配合EZ1 RNA Cell Mini Kit、EZ1 RNA Tissue Mini Kit或EZ1 RNA Universal Tissue Kit获得的高品质RNA适用于各种下游应用，包括：

应用real-time RT-PCR进行基因表达分析
应用芯片技术进行基因表达分析
肿瘤研究

资源

For purification of total RNA from tissue and cells using EZ1 instruments

FAQ

Operation of the BioRobot EZ1 is so easy that no additional training other than the initial demo is needed. Operating the instrument requires only three major steps:

Insert the appropriate EZ1 Protocol Card, switch on the workstation, and, if necessary, select the appropriate protocol
Follow the instructions on the liquid-crystal display (LCD), which describes where the sample tubes, elution tubes, filter tips, and EZ1 Reagent Cartridges must be loaded and which indicates when to start the protocol run
Collect purified nucleic acids when the protocol run ends

FAQ ID -1239

No, the new EZ1 RNA Kits can only be used with updated protocols programmed on the new EZ1 RNA Card V2.0.

The new card contains all protocols available for the isolation of RNA on the BioRobot EZ1 with the new kits:

Purification of total RNA from cells using the EZ1 RNA Cell Mini Kit, with and without DNase digest.
Purification of total RNA from standard tissue using the EZ1 RNA Tissue Mini Kit, with and without DNase digest.
Purification of total RNA from any type of tissue using the EZ1 RNA Universal Tissue Kit, with and without DNase digest.

Likewise, the former EZ1 RNA Cell Mini Kit (cat. no. 958134), EZ1 RNA Tissue Mini Kit (cat. no. 959134) and EZ1 RNA Universal Tissue Kit (cat. no. 956136) are not compatible with the new EZ1 RNA Card (cat. no. 9015590).

FAQ ID -1025

Yes, please follow the Supplementary Protocol 'Isolation of total nucleic acids from animal and human cells using the EZ1 RNA Cell Mini Kit'

FAQ ID -974

Yes, please follow the Supplementary Protocol 'Isolation of total nucleic acids from animal and human tissues using the EZ1 RNA Tissue Mini Kit'.

FAQ ID -975

Protocols for nucleic acid purification are supplied on pre-programmed EZ1 Protocol Cards. These protocols provide both on-screen instructions for the operator and operating commands for the workstation. The range of kits and protocols for the EZ1 is continuously expanding to allow nucleic acid purification from a wide range of sample types. Please browse our Supplementary Protocol Section to find specialized EZ1 protocols for a large variety of clinical sample types, in addition to the standard sample types covered in our EZ1 handbooks.

FAQ ID -1241

Loading of sample tubes, elution tubes, filter-tips, and Reagent Cartridges onto the worktable of the BioRobot EZ1 is intuitive, easy and quick. Details about worktable setup are provided with each new protocol, and are also displayed in the liquid-crystal display (LCD) of the control panel when you start setup of the worktable.

FAQ ID -1243

No, prefilled Reagent Cartridges can be loaded onto the BioRobot EZ1 with their seals. During operation of the instrument, the piercing unit, a row of 6 metal spikes located behind the tip adapters, will puncture the foil of the cartridges, thereby exposing the reagents.

FAQ ID -1240

For RNA isolated on the BioRobot EZ1 and BioRobot M48:

The RNA can be directly applied to the Agilent Bioanalyzer, since it is being denatured in the final protocol steps of these isolation procedures.

For RNA prepared with all other QIAGEN RNA Isolation Products:

We recommend to denature the samples in a water bath for 2 min at 70°C, and then place them directly on ice prior to loading them onto the Agilent Bioanalyzer.

FAQ ID -528

Yes, please follow the User-Developed Protocol 'Purification of total RNA from paraffin-embedded sections using the EZ1 RNA Tissue Mini Kit' (MA33).

FAQ ID -976

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ ID -12

No extra accessories need to be purchased for the BioRobot EZ1, as sample and elution tubes, as well as tips and tip holders are included with each EZ1 DNA or RNA isolation kit.

FAQ ID -1246