Microbial DNA-Free Water

配合Microbial DNA qPCR产品使用

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Microbial DNA-Free Water

Cat. No. / ID: 338132

12 tubes of 1.35 ml each

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Microbial DNA-Free Water 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的（再）订购

特点

使用Microbial DNA qPCR Assays通过PCR实验对其进行了严格检测
使用0.22 μM的过滤器进行了过滤灭菌
经过紫外线处理，消除基因组DNA

产品详情

Microbial DNA-Free Water为分子生物级纯度、不含核酸的纯水；其经过过滤灭菌，并通过紫外线处理去除基因组DNA。使用对细菌和真菌DNA十分敏感的一组Microbial DNA qPCR Assays对该纯水的质量进行PCR检测。如果所有对Microbial DNA-Free Water的PCR检测均未获得阳性结果，则说明该批次通过质量检测。这一严格的质控流程确保了Microbial DNA qPCR Assay或Array实验结果准确，不受干扰。

绩效

对所有批次Microbial DNA-Free Water进行质检，确定其不含微生物DNA。在产品附带的产品信息表中含有质检结果。

原理

严格的纯化和检测流程确保了Microbial DNA-Free Water为纯净的纯水，并不含基因组DNA。该纯水通过0.22 μl过滤器过滤灭菌，并经过紫外线处理，去除基因组DNA。完成纯化后，每批次纯水都使用Microbial DNA qPCR Assays进行质量检测，只有所有检测结果皆为阴性的批次能够通过质检，确保其不含细菌或真菌DNA。

应用

Microbial DNA-Free Water适合配合Microbial DNA qPCR Arrays和Assays使用，用于稀释浓缩的Microbial DNA Positive Control和Microbial qPCR Mastermix，也可作为无模板对照。

资源

Download Safety Data Sheets for QIAGEN product components.

For application-specific microbial identification and profiling by real-time PCR

For real-time PCR-based profiling/detection of microbial species, antibiotic resistance genes or virulence factor genes

FAQ

The Microbial DNA qPCR Assays for bacterial species detection use the 16S rRNA gene as the template. Microbial DNA qPCR Assays for detection of fungal species use ribosomal (small subunit or larger subunit) genes as the template. Microbial DNA qPCR Assays for detection of microbial genes use various antibiotic resistance genes and virulence factor genes for the template.

FAQ ID — 3403

Store Microbial DNA qPCR Array plates at room temperature.
Store Microbial DNA qPCR Assays, Microbial DNA qPCR Assay Kits, and Microbial DNA qPCR Multi-Assay Kits at –20°C.
Store Microbial qPCR Mastermix at –20°C.
Store Microbial DNA Positive Control at –20°C.
Store Microbial DNA-Free Water at –20°C.

FAQ ID — 3394

Limit of detection (LOD) is defined as the lowest concentration at which 95% of positive samples are detected. Since LOD is not necessarily within the linear range of an assay, LOD can be lower than LLOQ.

FAQ ID — 3402

The Microbial DNA qPCR Arrays and Assay Kits do not allow measurement of antibiotic resistance gene expression because reverse transcriptase enzyme is not included. Microbial DNA qPCR Assays have not been tested on cDNA.

FAQ ID — 3404

The lower limit of quantification (LLOQ) is the lower limit at which an assay can provide quantitative results. It is the lowest template concentration that is within the linear range of the assay.

FAQ ID — 3401

Most of the Microbial DNA qPCR Assays that detect bacterial species are species-specific. Assays that do detect more than one species will list which species are detected in the product specification sheet. These were determined by both experimental data and in silico prediction.

FAQ ID — 3400

The Microbial qPCR Mastermix (ROX) is suitable for use with the following real-time cyclers: all Applied Biosystems and Agilent (formerly Stratagene); BioRad Opticon, Opticon 2, and Chromo 4; Roche LightCycler 480; and Eppendorf Mastercycler ep realplex 2, 2S, 4, and 4S.

The Microbial qPCR Mastermix (Fluorescein) is suitable for use with the following real-time cyclers: BioRad iCycler, MyiQ, and iQ5.

FAQ ID — 3395