QIAamp DNA FFPE Tissue Kit

从石蜡包埋组织中纯化基因组DNA

特点

快速纯化高品质、即用型DNA
重复性好，产量高
完全去除污染物和抑制剂

产品详情

QIAamp DNA FFPE Tissue Kit专为从福尔马林固定、石蜡包埋组织中纯化DNA而设计。该试剂盒应用特殊的裂解方式脱离组织切片中的DNA，克服了福尔马林交联造成的抑制效应。试剂盒通过QIAamp MinElute离心柱纯化高品质DNA，洗脱体积小。QIAamp DNA FFPE Tissue Kit可在QIAcube全自动核酸纯化仪上自动纯化DNA。

绩效

QIAamp DNA FFPE Tissue Kit纯化的DNA适用于多种下游应用，如但核苷酸多态性（SNP）、短串联重复序列（STR）基因分型和药物基因组学研究，也可用于PCR（参见""）。

查看图表

PCR analysis of DNA purified from FFPE tissue samples.

原理

QIAamp DNA FFPE Tissue Kit使用成熟的QIAamp MinElute技术，从小体积样本中纯化基因组DNA和线粒体DNA。该试剂盒同时具备硅胶膜选择性结合DNA的特性，以及20 µl到100 µl的灵活洗脱体积。

经专门优化的裂解条件，无需过夜温育即可从福尔马林固定、石蜡包埋组织中高效纯化基因组DNA。蛋白酶K消化后在较高的温度温育，部分去除游离DNA的福尔马林交联，提高DNA产量和纯度，更适用于下游应用。需要注意的是，从福尔马林固定、石蜡包埋样本中分离的DNA分子量通常低于新鲜或冷冻样本中的DNA。DNA片段化的程度取决于样本类型、储存时间以及固定的条件。

程序

QIAamp DNA FFPE Tissue Kit流程包括6个步骤：去除石蜡、裂解、加热、结合、洗涤和洗脱（参见" Procedure"）。样本裂解后，通过简便的QIAamp DNA FFPE Tissue流程，在30分钟内即可得到高纯DNA，特别适用于同时处理多个样本。

查看图表

QIAamp DNA FFPE Tissue Kit procedure.

应用

QIAamp DNA FFPE Tissue Kit专为从福尔马林固定、石蜡包埋的组织样本中纯化DNA而设计。

辅助数据和图表

PCR analysis of DNA purified from FFPE tissue samples.

Genomic DNA was purified from different FFPE tissue samples using the QIAamp DNA FFPE Tissue Kit. Products of the prnp gene (78 bp, 464 bp, and 727 bp) were amplified by real-time quantitative PCR.

Specifications

Features	Specifications
ApplicationsZH	Real-time PCR, STR analysis, LMD-PCR
Processing	Manual
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	Genomic DNA, mitochondrial DNA
Elution volume	20-100 µl
Format	Spin column
Technology	Silica technology
Main sample type	Formalin fixed paraffin embedded tissue samples
Sample amount	Up to 8 sections, each with a thickness of up to 10 µm and a surface area of up to 250 mm2

资源

FAQ

The MinElute spin columns included in the following kits should be stored at 2–8°C upon arrival: AllPrep DNA/RNA Micro, EpiTect Fast DNA Bisulfite, EpiTect Fast FFPE Bisulfite, EpiTect Fast LyseAll Bisulfite, EpiTect Plus DNA Bisulfite, EpiTect Plus FFPE Bisulfite, EpiTect Plus LyseAll Bisulfite, exoRNeasy Serum/plasma Maxi, exoRNeasy Serum/Plasma Midi, GeneRead DNA FFPE, GeneRead rRNA Depletion, GeneRead Size Selection, MinElute Gel Extraction, MinElute PCR Purification, MinElute Reaction Cleanup, miRNeasy FFPE, miRNeasy Micro, miRNeasy Serum/Plasma, QIAamp DNA FFPE, QIAamp DNA Investigator, QIAamp DNA Micro, QIAamp MinElute Media, QIAamp MinElute Virus Spin, QIAamp MinElute Virus Vacuum, RNeasy FFPE, RNeasy Micro, RNeasy Plus Micro.

Short-term storage (up to 4 weeks) at room temperature (15–25°C) does not affect the performance. However, for optimal performance and quality, storage temperature should not exceed 25°C.

FAQ ID - 3560

The composition of Buffer ATE is:

- 10 mM Tris-Cl pH 8.3

- 0.1 mM EDTA

- 0.04% NaN3 (sodium-azide)

FAQ ID -3122

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

FAQ ID -728