FlexiGene DNA Kit

在单管中,从全血、白膜层和培养细胞中纯化基因组DNA

S_1624_RPA_QA_1070

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FlexiGene DNA Kit (250)

Cat. No. / ID:   51206

For isolation of genomic DNA from whole blood, buffy coat, and cultured cells in a single tube with fast kit preparation
This product contains substances regulated under REACH (EC 1907/2006 Annex XIV). The use of this product in the EU is permitted subject to an exemption (Article 56(3)). Please refer to the REACH notification and the SDS of this product, both of which can be found in the “Resources” section of this page, for more information.
FlexiGene DNA Kit 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的(再)订购

特点

  • 容易处理,无样本混淆的风险
  • 可从0.1–20 ml全血中纯化
  • 可从全血、白膜层和培养细胞中直接纯化
  • 纯化速度快
  • 无需有机提取

产品详情

FlexiGene体系可通过简单的离心操作从人全血、白膜层和培养细胞中分离高纯度的DNA。FlexiGene DNA Kit包括缓冲液和QIAGEN蛋白酶,可直接在单管中简单的进行纯化。联系Autogen, Inc.,咨询FlexiGene试剂的自动纯化解决方案。

绩效

使用FlexiGene体系纯化的DNA可达150 kb(参见" High-quality genomic DNA")。可用于扩增反应和Southern印迹实验,也可存档(参见" Efficient downstream PCR analysis")。

DNA在单管中通过离心操作,从0.1 – 20 ml(血液)、0.1 – 2 ml(白膜层)或1 – 2 x 106个细胞(培养细胞)中纯化DNA。1小时内处理12个样本。依据样本大小,再水合体积0.2 – 1 ml。

使用FlexiGene系统从不同类型的样本中纯化获得的DNA产量
样品     体积 
DNA 产量(µg)
血液*  
0.3 ml
11–14
血液*  
2.0 ml
75–90
血液*  
10.0 ml
 
380–460
白膜层  
0.5 ml
 
110–130
HeLa细胞  
106 cells
 
25–35
* 全血以正常的白细胞计数(7x106 个细胞/ml)。

查看图表

原理

FlexiGene DNA体系可从不同量的全血、白膜层和培养细胞中纯化基因组DNA。纯化过程在单管中进行,费用少,操作简单。纯化的DNA在多种下游应用中表现良好。

程序

首先在样本中加入裂解液(参见" FlexiGene DNA procedure")。细胞核和线粒体离心沉于底部,使用包含QIAGEN Protease的变性缓冲液重新悬浮。然后蛋白裂解,加入异丙醇沉淀DNA,离心处理,70%乙醇洗涤和干燥。使用水合缓冲液重新悬浮的DNA可即用于下游分析或–20°C贮存。可从全血、白膜层和培养细胞中获得高产量的DNA。
查看图表

应用

FlexiGene DNA Kit纯化的DNA可用于多种下游应用,包括:

  • PCR
  • Southern印迹
  • 存档

辅助数据和图表

Specifications

FeaturesSpecifications
ApplicationsZHPCR techniques, restriction digest, Southern blotting, sequencing
Sample amount100 µl - 20 ml
FormatScalable
Time per run or per prep25 - 45 minutes (+ DNA rehydration)
Main sample typeWhole blood, buffy coat, cultured cells
TechnologySingle tube precipitation method
ProcessingManual
Yield35 µg / ml whole blood
Purification of total RNA, miRNA, poly A+ mRNA, DNA or proteinGenomic DNA
Elution volume200 µl - 1000 µl

资源

试剂盒操作手册 (1)
For purification of DNA from human whole blood, buffy coat, cultured cells
补充实验方案 (1)
This protocol is designed for purification of DNA from up to 10 ml clotted blood using the FlexiGene DNA Kit.
其他资源 (1)
7 years storage at 2-8°C and -20°C
安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
Certificates of Analysis (1)

FAQ

Do you have a protocol for purification of DNA from clotted blood using the FlexiGene DNA Kit?

Yes, please follow the User-Developed Protocol 'Purification of DNA from clotted blood using the FlexiGene DNA Kit' (FG01).

 

FAQ ID -973
Do you have stability data for genomic DNA isolated with the Flexigene DNA Kit?

Yes. We have shown that DNA purified with the Flexigene DNA Kit is stable for at least 7 years when being stored at either 2-8°C or -20°C.  However, the DNA stability is dependent upon storage conditions.  The data is available here under Additional Resources.

 

FAQ ID -1317
Do you have a protocol for the isolation of genomic DNA from frozen clotted blood?

Yes, we have the following protocols:

  • Isolation of genomic DNA from frozen clotted whole blood using the QIAGEN Genomic-tip 100/G (QG02). TEST

You will need to prepare the required buffers according to the recipes in Appendix A of the QIAGEN Genomic DNA Handbook, or you can purchase the Genomic DNA Buffer Set containing pre-made solutions. Alternatively, the QIAGEN Blood & Cell Culture Midi Kit, containing Genomic-tips 100/G and buffers, can be used.

  • Purification of archive-quality DNA from clotted whole blood using Clotspin Baskets and the Gentra Puregene Blood Kit (PG03).
  • Purification of archive-quality DNA from clotted whole blood using the Gentra Puregene Tissue Kit or Gentra Puregene Mouse Tail Kit (PG04).
  • Purification of DNA from clotted blood using the FlexiGene DNA Kit (FG01).
FAQ ID -900. Test
What is the composition of Buffer FG3?
Buffer FG3, the hydration buffer supplied in the FlexiGene DNA Kit, is a 10 mM Tris-Cl solution at pH 8.5.
FAQ ID -356