QIAamp DNA FFPE Tissue Kit

从石蜡包埋组织中纯化基因组DNA

S_0491_AT_QA_CMYK

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QIAamp DNA FFPE Tissue Kit (50)

Cat. No. / ID:   56404

For 50 DNA preps: 50 QIAamp MinElute Columns, Proteinase K, Buffers, Collection Tubes (2 ml) Use our next-generation QIAamp DNA FFPE Advanced Kits for improved performance.
SGD 778.00
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QIAamp DNA FFPE Tissue Kit 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的(再)订购

特点

  • 快速纯化高品质、即用型DNA
  • 重复性好,产量高
  • 完全去除污染物和抑制剂

产品详情

QIAamp DNA FFPE Tissue Kit专为从福尔马林固定、石蜡包埋组织中纯化DNA而设计。该试剂盒应用特殊的裂解方式脱离组织切片中的DNA,克服了福尔马林交联造成的抑制效应。试剂盒通过QIAamp MinElute离心柱纯化高品质DNA,洗脱体积小。QIAamp DNA FFPE Tissue Kit可在QIAcube全自动核酸纯化仪上自动纯化DNA。

绩效

QIAamp DNA FFPE Tissue Kit纯化的DNA适用于多种下游应用,如但核苷酸多态性(SNP)、短串联重复序列(STR)基因分型和药物基因组学研究,也可用于PCR(参见" PCR analysis of DNA purified from FFPE tissue samples")。
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原理

QIAamp DNA FFPE Tissue Kit使用成熟的QIAamp MinElute技术,从小体积样本中纯化基因组DNA和线粒体DNA。该试剂盒同时具备硅胶膜选择性结合DNA的特性,以及20 µl到100 µl的灵活洗脱体积。

经专门优化的裂解条件,无需过夜温育即可从福尔马林固定、石蜡包埋组织中高效纯化基因组DNA。蛋白酶K消化后在较高的温度温育,部分去除游离DNA的福尔马林交联,提高DNA产量和纯度,更适用于下游应用。需要注意的是,从福尔马林固定、石蜡包埋样本中分离的DNA分子量通常低于新鲜或冷冻样本中的DNA。DNA片段化的程度取决于样本类型、储存时间以及固定的条件。

程序

QIAamp DNA FFPE Tissue Kit流程包括6个步骤:去除石蜡、裂解、加热、结合、洗涤和洗脱(参见" Procedure")。样本裂解后,通过简便的QIAamp DNA FFPE Tissue流程,在30分钟内即可得到高纯DNA,特别适用于同时处理多个样本。

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应用

QIAamp DNA FFPE Tissue Kit专为从福尔马林固定、石蜡包埋的组织样本中纯化DNA而设计。

辅助数据和图表

Specifications

FeaturesSpecifications
ApplicationsZHReal-time PCR, STR analysis, LMD-PCR
ProcessingManual
Purification of total RNA, miRNA, poly A+ mRNA, DNA or proteinGenomic DNA, mitochondrial DNA
Elution volume20-100 µl
FormatSpin column
TechnologySilica technology
Main sample typeFormalin fixed paraffin embedded tissue samples
Sample amountUp to 8 sections, each with a thickness of up to 10 µm and a surface area of up to 250 mm2

资源

产品介绍与指南 (5)
Sample to Insight solutions for successful molecular analysis
High-quality, nucleic acid purification for successful PCR and NGS experiments.
Second edition — innovative tools
Critical factors for molecular analysis of FFPE samples
试剂盒操作手册 (1)
安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
Certificates of Analysis (1)

FAQ

I received a kit containing the MinElute columns; however, they were left out for a while and not stored at 2–8°C upon receipt. Can I still use them?

The MinElute spin columns included in the following kits should be stored at 2–8°C upon arrival: AllPrep DNA/RNA Micro, EpiTect Fast DNA Bisulfite, EpiTect Fast FFPE Bisulfite, EpiTect Fast LyseAll Bisulfite, EpiTect Plus DNA Bisulfite, EpiTect Plus FFPE Bisulfite, EpiTect Plus LyseAll Bisulfite, exoRNeasy Serum/plasma Maxi, exoRNeasy Serum/Plasma Midi, GeneRead DNA FFPE, GeneRead rRNA Depletion, GeneRead Size Selection, MinElute Gel Extraction, MinElute PCR Purification, MinElute Reaction Cleanup, miRNeasy FFPE, miRNeasy Micro, miRNeasy Serum/Plasma, QIAamp DNA FFPE, QIAamp DNA Investigator, QIAamp DNA Micro, QIAamp MinElute Media, QIAamp MinElute Virus Spin, QIAamp MinElute Virus Vacuum, RNeasy FFPE, RNeasy Micro, RNeasy Plus Micro.

Short-term storage (up to 4 weeks) at room temperature (15–25°C) does not affect the performance. However, for optimal performance and quality, storage temperature should not exceed 25°C.

FAQ ID - 3560
What is the composition of elution buffer ATE in the QIAamp DNA Investigator kit, QIAamp DNA FFPE Tissue kit and the QIAamp Fast DNA Stool Mini kit?

The composition of Buffer ATE is:

- 10 mM Tris-Cl pH 8.3

- 0.1 mM EDTA

- 0.04% NaN3 (sodium-azide)

FAQ ID -3122
What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

FAQ ID -728