DNeasy mericon Food Kit
用于从食品原料或加工过的食品中纯化高品质DNA
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Features
- 经改善的CTAB实验流程，加快对食品样本的处理速度
- 从加工食品中高效纯化DNA、甚至是片段化的DNA
- 纯化复杂食品样本的DNA时不残留PCR抑制剂
- 提供专用于深加工食品的实验方案
Product Details
DNeasy mericon Food Kit可从多种食品原料或加工食品中纯化高品质DNA。这些食品样本的PCR抑制剂残留量被最小化。该试剂盒是QIAGEN食品检测产品线的一部分，QIAGEN还提供病原体DNA检测、转基因生物DNA检测和成分鉴定试剂盒。
Performance
在食品生产制造过程中，热量、辐射、高压和pH值的变化都会导致DNA降解和片段化。DNeasy mericon Food Kit含有优化的试剂，能够回收短的DNA片段（达100 bp），确保高度片段化的DNA也能够被高效分离，并在PCR反应中得到扩增。DNeasy mericon Food Kit为适用范围广范的DNA分离方法，即使处理PCR抑制剂含量高、经过深加工的、多脂、酸性、高或低DNA含量的食品，也能获得出色、可靠的结果（参见" High yields of DNA and minimal inhibitor carryover"和" Heightened resistance to inhibitors"）。此外，您无需再为不同的食品样本进行不同的裂解流程。
See figures
Principle
DNeasy mericon Food Kit采用改善的十六烷基三甲基溴化铵（CTAB）核酸提取技术。
CTAB为非离子型洗涤剂，被广泛用于从多种类型的组织中高效纯化总细胞核酸。在低盐环境中，CTAB会与核酸形成复合物；在高盐环境中，如Food Lysis Buffer中，CTAB会与多糖、蛋白或植物代谢物等细胞抑制剂形成复合物。
我们的CTAB实验方案比传统方案精简了若干流程，2.5小时内可处理多达30个样本（参见" Efficient and rapid DNA extraction"）。该试剂盒能够高产量、高效的纯化DNA。
See figures
Procedure
DNeasy mericon Food Kit的实验方案经过优化，结合CTAB方法和Proteinase K消化，首先消化完整的组织，然后沉淀蛋白和其他食物内细胞抑制剂。
抑制剂通过离心沉淀，纯化DNA仍留在溶液中。在随后的氯仿萃取中，没有沉淀的残留CTAB-protein、CTAB-细胞碎片、或CTAB-多糖复合物和其他亲脂性抑制剂被萃取到氯仿有机相，而被去除。稍后只会处理不含抑制剂、仅含有DNA的水相。将水相与结合缓冲液（调整结合条件）混合，并上样至QIAquick Spin Columns。获得的DNA可即用于mericon real-time PCR分析。
Applications
DNeasy mericon Food Kit可从多种食品原料或加工食品中快速（2.5小时可处理30个样本）纯化DNA，且复杂食品样本中的PCR抑制剂的残留量降低至最低水平。
Supporting data and figures
High yields of DNA and minimal inhibitor carryover.
Samples of peanut butter and chocolate were prepared using the DNeasy mericon Food Kit (standard protocol) and products from 3 other suppliers to amplify a segment of the tnrL chloroplast tRNA gene. (A) Average quantification value (Cq) is substantially lower for samples prepared with the DNeasy mericon Food Kit compared to the other suppliers. (B) Using inhibitor-free DNA, this assay gives a positive Cq shift between 2.9–3.5 when samples are diluted 1:10; lower shifts indicate the presence of inhibitors. To test for inhibitors carried over from the sample preparation procedure, samples were diluted 1:10 and the same DNA segment was amplified. Both food samples were inhibitor-free when prepared with the DNeasy mericon Food Kit. Samples prepared with products from the other suppliers showed strong inhibitor contamination.
Resources
试剂盒操作手册 (1)
安全数据表 (1)
产品介绍与指南 (3)
Certificates of Analysis (1)
Safety Data Sheets (2)
Brochures & Guides (3)
Kit Handbooks (1)
FAQ
Why is my 260/280 ratio low after using the DNeasy mericon Food Kit?
Does salt concentration in the food matter when using the DNeasy mericon Food Kit?
CAn I use milk or other liquids with the DNeasy mericon Food kit? What volume would I use?
Why does the DNeasy mericon Food Kit use a QIAquick column and Buffer PB rather than a DNeasy column and Buffer AL, which might be expected since the kit isolates genomic DNA?