You can accept or revoke the cookies used on this website at any time with the selection below or by adjusting your cookie settings. For complete details about our cookies, see our Cookie Policy.
生物样本首先在含有异硫氰酸胍的变性缓冲液中裂解并匀浆,DNase和RNase立即失活,可确保DNA和RNA的完整性。将裂解物通过AllPrep DNA Mini纯化柱。在高盐缓冲液中,纯化柱与基因组DNA选择性高效结合。在优化的缓冲液条件下进行柱上Proteinase K消化,可从各种类型样本中纯化获得高产量DNA。之后洗脱获得即用型DNA。在AllPrep DNA Mini纯化柱的流出液中加入乙醇,用Proteinase K进行消化。消化后再次加入乙醇,包括miRNA在内的总RNA结合到RNeasy Mini纯化柱上。用DNase I消化获得高产量RNA,且不含DNA。洗涤步骤可冲走污染物,之后可洗脱获得高品质RNA。
AllPrep操作流程仅需75分钟,即可快速、便利的纯化获得高品质核酸,可即用于多种下游应用(参见"The fast and convenient AllPrep procedure")。该试剂盒使用创新的试剂,因此无需使用苯酚等有毒物质,极大减少了对环境和使用者的危害。AllPrep DNA/RNA/miRNA Universal Kit可在QIAcube全自动核酸纯化仪上实现自动化,确保流程标准化,为您带来更大的便利。
To visualize contamination of RNA with genomic DNA, quantitative real-time PCR assays were performed with (+RT) or without (–RT) reverse transcriptase. Unlike kits from other suppliers, the AllPrep DNA/RNA/miRNA Universal Kit delivered high yields of pure RNA from difficult-to-lyse adipose tissue, as demonstrated by c-Jun expression assays.