PAXgene Blood DNA Kit

从全血样本中纯化至多500 μg基因组DNA

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PAXgene Blood DNA Kit (25)

目录编号 / ID. 761133

Processing tubes and buffers for 25 preparations

需咨询

仅作研究用途。不用于诊断程序。任何表述或表示都并非旨在提供诊断、预防或治疗疾病的信息。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的（再）订购

特点

同一体系中进行血液收集和纯化
易于操作
提高工作流程的效率

产品详情

PAXgene Blood DNA系统包括用于血液采集和稳定的PAXgene Blood DNA Tubes和用于单管纯化DNA的PAXgene Blood DNA Kit。

绩效

PAXgene Blood DNA System分离的DNA A₂₆₀/A₂₈₀比值在1.7–1.9。片段大小20–200 kb，平均长度50–150 kb（参见""）。DNA平均产量为150–500 µg，由血样中的核细胞数目决定。纯化的DNA可用于PCR、定量和real-time PCR（参见""）。

原理

PAXgene Blood DNA System是整合的标准化体系，用于收集和稳定全血样本并分离基因组DNA。该体系通过PAXgene Blood DNA Tubes收集和稳定血液，并使用PAXgene Blood DNA Kit纯化DNA。

PAXgene Blood DNA Tubes包含可防止血液凝结以及可稳定白细胞的混合试剂，用来收集血液。血液样本可在室温下在管中储存长达14天。用PAXgene Blood DNA Kit中提供的缓冲液获得的高纯度的基因组DNA适合各种下游应用。

程序

PAXgene Blood DNA操作步骤如流程图" PAXgene Blood DNA procedure"所示。血液样本（8.5 ml）由PAXgene Blood DNA Tubes收集，并储存或运输。如需纯化DNA，则将血液转入处理管中（已预充细胞裂解缓冲液），将溶液混合，裂解红细胞和白细胞。细胞核和线粒体通过离心收集、洗涤，重悬于裂解缓冲液中。蛋白污染物经蛋白酶孵育后去除。DNA用异丙酮沉淀、70%乙醇洗涤、干燥，并重悬于缓冲液。

应用

纯化的DNA可用于多种下游应用，包括：

PCR和定量real-time PCR
Southern印迹
SNP基因分型
药物基因组研究
SNP发现和鉴定

辅助数据和图表

PAXgene Blood DNA procedure.

Blood samples (8.5 ml) are collected in PAXgene Blood DNA Tubes, where they may be stored or transported. For DNA isolation, the blood is transferred to processing tubes (supplied already filled with cell lysis buffer), and the solution is mixed to lyse red and white blood cells. Cell nuclei and mitochondria are pelleted by centrifugation, washed, and resuspended in digestion buffer. Protein contaminants are removed by incubation with a protease. DNA is precipitated in isopropanol, washed in 70% ethanol, dried, and resuspended in resuspension buffer.

Efficient multiplex PCR of 3 mitochondrial genes.

High quality and high molecular weight of genomic DNA.

规格

特点	规格
Main sample type	Whole blood
Time per run	60 min/8 samples
Elution volume	1 ml
Yield	150–500 µg
Format	Processing tube
Sample amount	8.5 ml
ApplicationsZH	(RT-)PCR, (RT-)qPCR, NGS, genotyping, microarray analysis
Processing	Manual (centrifugation)

资源

For collection, storage, and transport of whole blood for genomic DNA isolation

Second edition — innovative tools

Download Safety Data Sheets for QIAGEN product components.

Groelz et al., ASHG 2004

For isolation of genomic DNA from 8.5 ml human whole blood

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Transplant

Infectious Disease

Oncology

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Sample Processing

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