Allprotect Tissue Reagent

迅速稳定组织中的DNA、RNA和蛋白质

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Allprotect Tissue Reagent (100 ml)

Cat. No. / ID: 76405

100 ml Allprotect Tissue Reagent, Allprotect Reagent Pump

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Allprotect Tissue Reagent 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的（再）订购

特点

无需液氮或干冰
立即方便地稳定收集到的组织
可长期储存组织，用于后续分析
标准的样本制备流程，用于系统生物学研究

产品详情

Allprotect Tissue Reagent可在室温下立即稳定组织样本中的DNA、RNA和蛋白质。这样可以稳定DNA、RNA和蛋白质，以保证可靠的下游分析。稳定后的组织可在15–25°C运输7天，2–8°C储存长达12个月，在–20°C或–80°C可储存更长时间。

绩效

使用Allprotect Tissue Reagent即刻稳定采集的组织可保留DNA、RNA和蛋白质在体内的性质，保证了可靠的下游分析，稳定的组织可在室温（15–25°C）下储存长达7天（参见""、""和""）。稳定RNA十分重要，因为转录水平会在组织采集后快速变化（参见""和""）。使用Agilent 2100 Bioanalyzer分析Allprotect Tissue Reagent稳定组织得到的RNA，RIN值较高，表明RNA较完整。Allprotect Tissue Reagent还可稳定microRNA、甲基化的基因组DNA和磷酸化蛋白。

查看图表

Intact protein for western blotting.

Prevention of protein degradation.

Preservation of protein activity.

Prevention of gene induction.

Prevention of RNA degradation.

原理

将刚采集的组织浸没在Allprotect Tissue Reagent中，可即刻稳定DNA、RNA和蛋白质，并保存其在体内的特性。Allprotect Tissue Reagent稳定的组织样本可长期储存，无需冷冻，在室温下可储存长达7天，或在2–8°C储存长达12个月。

该试剂是QIAGEN系统生物学样本制备技术产品线的一部分，可与AllPrep DNA/RNA/Protein Mini Kit配合使用，从同一个珍贵的样本中同时纯化DNA、RNA和蛋白质。Allprotect稳定的组织可用QIAGEN的DNeasy、QIAamp、RNeasy和Qproteome等试剂盒纯化DNA、RNA或蛋白质。用于酶分析的功能性蛋白可使用Qproteome Mammalian Protein Prep Kit从Allprotect稳定的组织中纯化获得。无需在纯化前将样本分为3份，因此可最大程度的回收DNA、RNA和蛋白质。此外，纯化过程无需加入苯酚和丙酮等有机溶剂。DNA、RNA和蛋白质皆来自同一个样本，减少了从不同样本中制备这些待分析物可能带来的差异。

QIAGEN还提供分别纯化DNA、RNA和蛋白质的产品（见表）。包括Qproteome Mammalian Protein Prep Kit，可从Allprotect稳定的组织中纯化功能蛋白。使用Qproteome Mammalian Protein Prep Kit从Allprotect稳定的组织中纯化的蛋白质可用于多种下游应用。

可与Allprotect Tissue Reagent配合使用的QIAGEN试剂盒
待纯化的分析物	推荐的试剂盒
基因组DNA、总RNA和总蛋白质	AllPrep DNA/RNA/Protein Mini Kit
基因组DNA和总RNA	AllPrep DNA/RNA Micro Kit AllPrep DNA/RNA Mini Kit
基因组DNA	DNeasy Blood & Tissue Kit QIAamp DNA Mini Kit
总RNA	RNeasy Plus Micro Kit（适用于小的组织样本）RNeasy Mini Kit（适用于易裂解组织） RNeasy Plus Mini Kit （适用于易裂解组织） RNeasy Fibrous Tissue Mini Kit （适用于富含纤维的组织） RNeasy Lipid Tissue Mini Kit（适用于富含脂肪的组织） RNeasy Microarray Tissue Mini Kit（适用于微芯片分析中的组织样本）
总蛋白质	Qproteome Mammalian Protein Prep Kit

程序

将刚采集的组织浸没在Allprotect Tissue Reagent中，以即刻稳定DNA、RNA和蛋白质（参见" Mechanism of Allprotect stabilization"）。稳定过程在室温下即可进行，无需使用危险的液氮或干冰。使用Allprotect Tissue Reagent即刻稳定采集的组织可保留DNA、RNA和蛋白质在体内的性质，保证了可靠的下游分析。

Allprotect Tissue Reagent稳定的组织可在室温下储存长达7天，在2–8°C的条件下储存长达12个月， DNA、RNA和蛋白质不会降解。在–20°C或–80°C可储存更长时间。

查看图表

Mechanism of Allprotect stabilization.

应用

使用AllPrep DNA/RNA/Protein Mini Kit可从Allprotect稳定的组织中同时纯化DNA、RNA和蛋白质。将Allprotect和AllPrep样本制备技术配合，可标准化系统生物学研究中的样本制备流程。使用AllPrep DNA/RNA/Protein Mini Kit纯化得到的基因组DNA和总RNA在real-time PCR和RT-PCR等下游应用中表现出色。使用该试剂盒纯化得到的总蛋白质适用于SDS-PAGE和Western印迹。

辅助数据和图表

Preservation of protein activity.

Rat liver was stored in Allprotect Reagent before total protein was purified using the Qproteome Mammalian Protein Prep Kit. Acidic phosphatase activity was similar to that of protein from control liver stored in liquid nitrogen. Each bar shows the mean from two liver pieces assayed in duplicate.

资源

Download Safety Data Sheets for QIAGEN product components.

For immediate stabilization of DNA, RNA, and proteins in animal and human tissues, and subsequent storage

FAQ

Allprotect Tissue Reagent is optimized to protect RNA, DNA, and proteins in humans and animal tissues. However, Allprotect can also be used with plant tissues. If the plants leaves are waxy, the surface of the leaves can be scratched to allow Allprotect reagent to permeate the tissue.

FAQ ID -1574

No, we do not recommend performing morphological studies with tissues that were incubated in Allprotect Tissue Reagent. The reagent causes the tissue to shrink and therefore changes its morphology.

FAQ ID -1571

We recommend using either the TissueRuptor or the TissueLyser for homogenization of tissues stored in Allprotect Tissue Reagent.

Since tissues incubated in Allprotect Tissue Reagent are harder than fresh tissues, longer homogenization times might be necessary (for details, see the Allprotect Tissue Reagent Handbook).

Furthermore, we do not recommend using rotor–stator homogenizers with a metal probe (e.g., Polytron) if proteins will be purified subsequently, as the metal probe can heat the sample.

FAQ ID -1572

No. Allprotect Tissue Reagent remains on the sample due to its viscosity. We recommend removing excess reagent by dabbing or rolling the sample over a paper towel.

However, residual Allprotect Tissue Reagent on the sample inhibits neither DNA, RNA, and/or protein purification, nor downstream applications.

FAQ ID -1573

In general, yes. Freezing and thawing a sample incubated in Allprotect Tissue Reagent several times does not affect analysis of RNA and protein in downstream applications.

However, if you would like to perform protein activity assays, you should test each specific assay, since several freeze–thaw cycles might reduce protein activity, depending on the protein being assayed.

FAQ ID -1575