QIAsymphony RGQ
用于整合的自动纯化、反应体系构建和检测，满足分子检测所需
Products
QIAsymphony SP核酸提取纯化分析仪应与可与其兼容的QIAsymphony Kits配合使用，适用于试剂盒使用手册中指定的应用。QIAsymphony AS应与可与其兼容的QIAGEN Kits配合使用，适用于试剂盒使用手册中指定的应用。如果将QIAsymphony AS 与非QIAGEN的试剂盒配合使用，用户应自行确认是否适用于相关应用。Rotor-Gene Q实时荧光定量PCR分析仪应与可与其兼容的QIAGEN Kits配合使用，适用于试剂盒使用手册中指定的应用。如果将Rotor-Gene Q实时荧光定量PCR分析仪与非QIAGEN的试剂盒配合使用，用户应自行确认是否适用于相关应用。
QIAsymphony DSP Virus/Pathogen Mini Kit
Cat. No. / ID: 937036
For 192 preps (200 µl each): includes 2 reagent cartridges and enzyme racks and accessories
特点
- 前所未有的灵活性
- 优化的操作流程，高实用性
- 易于操作，提供最大程度的便利性
- 监控分析和实验室开发测试
- 适合多种应用
产品详情
新的QIAGEN工作流程包括用于样本制备的QIAsymphony SP核酸提取纯化分析仪、用于反应体系构建的QIAsymphony AS以及在Rotor-Gene Q实时荧光定量PCR分析仪上进行的一系列即用型分析。QIAGEN将进一步研发、拓展QIAsymphony系列产品。
绩效
QIAsymphony RGQ配合artus QS-RGQ Kits，应用于多种高灵敏度、高特异性检测：
- CMV DNA（参见"Highly sensitive detection of CMV DNA"）
- EBV DNA（参见"Highly sensitive detection of EBV DNA"）
- HBV DNA（参见"Highly sensitive detection of HBV DNA"）
- HIV-1 RNA（参见"Highly sensitive detection of HIV-1 RNA"）
- HCV RNA（参见"Highly sensitive detection of HCV RNA"）
原理
QIAsymphony RGQ可处理多达96个样本，从样本到结果只需一次完整的自动化运行，节省时间。一系列专门的试剂盒可组成分子检测的开放或封闭系统。
QIAsymphony SP/AS仪器和Rotor-Gene Q实时荧光定量PCR分析仪配合形成了一个可自动完成工作流程的完整系统。QIAsymphony SP核酸提取纯化分析仪可自动完成各种类型样本的制备。QIAsymphony AS确保构建的PCR反应体系高度可靠且重复性好，适合每日需要进行复杂PCR分析的实验室。QIAsymphony AS可对每次运行或样本进行多重体系构建，支持artus QS-RGQ的产品，完全兼容CE-IVD标准的工作流程，也支持自己设定的工作流程。Rotor-Gene Q实时荧光定量PCR分析仪可配合CE-IVD-marked的artus QS-RGQ试剂盒或自己设定的工作流程进行敏感、精确的real-time PCR分析。
| 试剂盒 | 仪器 | 方法 |
|---|---|---|
| QIAsymphony DSP Virus/Pathogen Kits | QIAsymphony SP核酸提取纯化分析仪 | 从多种类型的样本中纯化病毒核酸或细菌DNA |
| artus QS-RGQ Kits | QIAsymphony AS | Real-time PCR（多重；样本和内参） |
| 应用 | 试剂盒 | 仪器 | 方法 |
|---|---|---|---|
| 纯化 | QIAsymphony DSP Virus/Pathogen Kits | QIAsymphony SP核酸提取纯化分析仪 | 从各种类型样本中纯化病毒核酸或细菌 |
| QIAsymphony DSP AXpH DNA Kit | QIAsymphony SP核酸提取纯化分析仪 | 从细胞培养悬浮液中纯化病毒核酸或细菌 | |
| QIAsymphony Certal Kits | QIAsymphony SP核酸提取纯化分析仪 | 从生物纯化缓冲液、细胞培养悬浮液样本和疫苗制备样本中纯化宿主残留DNA和病毒核酸 | |
| 反应体系构建 —病原体检测 | QuantiFast Pathogen +IC Kits | QIAsymphony AS | 使用序列特异性探针进行多重real-time PCR和RT-PCR |
| QuantiTect Virus Kits | QIAsymphony AS | 可在单管中高度灵敏的检测多种病毒DNA和RNA | |
| QIAGEN OneStep RT-PCR Kit | QIAsymphony AS | 使用任何RNA模板，简单设置，进行灵敏的RT-PCR | |
| 反应体系构建 —基因表达 | Rotor-Gene SYBR® Green Kits | QIAsymphony AS | 使用SYBR® Green I进行超快速、精确的real-time PCR和RT-PCR |
| Rotor-Gene Probe Kits | QIAsymphony AS | 使用序列特异性的探针进行超快速、精确的real-time PCR和RT-PCR | |
| Rotor-Gene Multiplex Kits | QIAsymphony AS | 使用序列特异性的探针进行超快速、精确的多重real-time PCR和RT-PCR | |
| 反应体系构建 —残留DNA 检测 | Certal Detection Kits | QIAsymphony AS | 多重real-time PCR检测残留DNA和内参 |
程序
QIAsymphony RGQ工作流程的开始是在QIAsymphony SP仪器上纯化核酸。从样本制备流程中得到的含纯核酸的洗脱液转移到QIAsymphony AS模块，进行分析体系构建。分析样本再转移到Rotor-Gene Q实时荧光定量PCR分析仪进行PCR和数据分析。
为了满足不同工作流程需要，操作系统中有两种模式：完整模式或独立模式。在完整模式中，样本在QIAsymphony SP核酸提取纯化分析仪处理后直接转移到QIAsymphony AS，减少了手工操作步骤和记录。为了增加灵活性，QIAsymphony SP核酸提取纯化分析仪和AS也可在独立模式中进行相互独立的操作。参见"QIAsymphony workflows"。
应用
QIAsymphony RGQ是目前和将来用于各种分子检测的最具有多功能性的系统。QIAsymphony RGQ在一个平台上整合了多种分析，也能进行自行开发测试。
适合于开放和闭合系统多种应用，包括：
- artus移植面板（CMV、EBV、HSV、BK病毒和VZV）
- artus血源性病毒面板（HIV、HCV和HBV）
- 内部测试
Specifications
| Features | Specifications |
|---|---|
| Elution volume | QIAsymphony SP, 50–400 µl (application/protocol dependent) |
| Features | Dynamic range, 12 orders of magnitude |
| Operating temperature | 15-32°C |
| Samples per run (throughput) | Tubes 0.2 ml; Strip Tubes 0.1 ml (4 tubes); Rotor-Disc 72; Rotor-Disc 100; Up to 100 samples per run using a Rotor-Disc 100 |
| Storage temperature | 5-40°C in manufacturer's package |
| Pollution level | 2 |
| Overvoltage category | II |
| Humidity | Relative humidity of 15–75% (noncondensing); maximum 75% relative humidity for temperatures up to 31°C, decreasing linearly to 50% humidity at 32°C |
| Instrument dimensions | QIAsymphony SP, 130 x 75 x 103 cm; QIAsymphony AS, 59 x 103 x 73 cm; QIAsymphony SP/AS (integrated operation), 185 x 103 x 73 cm |
| Processing | Automated |
| Heat dissipation/thermal load | Average, 0.183 kW (632 BTU/hour); Peak, 0.458 kW (1578 BTU/hour) |
| Input volume | QIAsymphony SP, 100-1000 µl (application/protocol dependent) |
| Optical System | Up to 6 channels spanning UV to infra-red wavelengths; Excitation sources: High energy light-emitting diodes; Detector: Photomultiplier; Acquisition time: 4 s. The software allows to create new excitation/detection wavelength combinations |
| Software | Rotor-Gene Q software, supplied on the installation CD provided |
| Technology | QIAsymphony SP — QIAGEN magnetic-particles chemistry |
| Power | 100-240 V AC, 50-60 Hz, 1400 VA, mains supply voltage are not to exceed 10% of nominal supply voltages |
| Place of operation | For indoor use only |
| Kits or Application Packs designed for this instrument | QIAsymphony SP — QIAsymphony DSP Virus/Pathogen Kits and other QIAsymphony Kits; QIAsymphony AS — artus QS-RGQ Kits (not available in all countries), Rotor-Gene Kits and other QIAGEN real-time and end-point PCR kits |
| Protocols/main application on this instrument | QIAsymphony SP — Purification of DNA, RNA, bacterial and viral nucleic acids, and 6xHis-tagged protein from a wide range of starting materials; QIAsymphony AS — PCR setup. |
| Thermal performance | Temperature range, 35 to 99ºC; Temperature accuracy, ±0.5ºC (type, measured 30 seconds after clock start); Temperature resolution, ±0.02ºC (smallest programmable increment); Temperature uniformity, ±0.02ºC; Ramp rate (peak ramp rates, air), >15ºC/s heating, >20ºC/s cooling |
| Typical run time | 40 cycles in 45 min with the QIAGEN RG Kits (assay dependent) |
| Weight | QIAsymphony SP, 175 kg; QIAsymphony AS, 90 kg; QIAsymphony SP/AS (integrated operation), 265 kg |
| Transportation conditions | -25-70°C in manufacturer's package |
| Warranty | 1 year on instrument; lifetime guarantee on excitation LEDs |
| Altitude | Up to 2000 m (6500 ft) |
资源
安全数据表 (1)
用户使用手册 (1)
耗材文档 (1)
应用与实验方案 (1)
实验方案软件 (1)
试剂盒操作手册 (1)
仪器技术参数 (1)
Certificates of Analysis (1)