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ipsogen JAK2 MutaSearch Kit (24)
Cat. No. / ID: 673813
For 24 reactions: V617F Positive Control, V617F Negative Control, V617F Cut-Off Sample, 2 Primers and Probe Mixes JAK2 wild-type and JAK2 V617F
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✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- 灵敏、准确的定量检测试剂
- 能够检测1%临界值的阳性样本
- 即用型解决方案，便利的工作流程
Product Details
ipsogen JAK2 MutaSearch Kit仅适用于科研，不可用于诊断。该试剂盒可用于Rotor-Gene Q实时荧光定量PCR分析仪或其他real-time PCR仪的real-time PCR实验。该试剂盒中的试剂经过优化，能够对基因组DNA中的JAK2 V617F/G1849T突变进行灵敏、可靠的检测。
Performance
为确保检测的高灵敏度，ipsogen JAK2 MutaSearch Kit已经过优化，利用1%的DNA临界样本（COS）、阳性和阴性对照，检测JAK2 V617F/G1849T突变（参见 Accurate detection of JAK2 V617F mutation, 1% cut-off sample, and positive and negative controls和 Reliable detection of wild-type JAK2, 1% cut-off sample, and positive and negative controls）。推荐的起始样本DNA最少为25 ng。
See figures
Principle
ipsogen JAK2 MutaScreen Kits为即用型试剂盒，能够对基因组DNA中SNP导致的JAK2 V617F/G1849T体细胞突变进行检测分析（参见 JAK2 mutation site identification）。该试剂盒灵敏度高，可重复性强。ipsogen JAK2 MutaScreen Kits提供JAK2 V617F的DNA检测临界样本（COS）、DNA阳性对照（PC）和阴性对照（NC）。使用检测临界样本和对照能够实现准确的基因分型分析。
See figures
Procedure
第一步是抽提总DNA，并检测其量与品质。第二步是通过real-time PCR扩增DNA。ipsogen JAK2 MutaSearch Kits能够对JAK2 V617F/G1849T突变进行灵敏的定量检测。只需按照试剂盒操作手册中的实验方案，即可便利的开始反应。
Applications
ipsogen JAK2 MutaSearch Kits能够对JAK2 V617F/G1849T突变进行灵敏、可靠的定量检测，仅适用于科研。
Supporting data and figures
JAK2 mutation site identification.
JAK2 mutation site identification.
Resources
试剂盒操作手册 (2)
安全数据表 (1)
Certificates of Analysis (1)
Kit Handbooks (1)
Safety Data Sheets (1)