FlexiGene DNA Kit
在单管中，从全血、白膜层和培养细胞中纯化基因组DNA
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FlexiGene DNA Kit (250)
Cat. No. / ID: 51206
For isolation of genomic DNA from whole blood, buffy coat, and cultured cells in a single tube with fast kit preparation
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FlexiGene DNA Kit 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。
✓ 全天候自动处理在线订单
✓ 博学专业的产品和技术支持
✓ 快速可靠的（再）订购
特点
- 容易处理，无样本混淆的风险
- 可从0.1–20 ml全血中纯化
- 可从全血、白膜层和培养细胞中直接纯化
- 纯化速度快
- 无需有机提取
产品详情
FlexiGene体系可通过简单的离心操作从人全血、白膜层和培养细胞中分离高纯度的DNA。FlexiGene DNA Kit包括缓冲液和QIAGEN蛋白酶，可直接在单管中简单的进行纯化。联系Autogen, Inc.，咨询FlexiGene试剂的自动纯化解决方案。
绩效
使用FlexiGene体系纯化的DNA可达150 kb（参见" High-quality genomic DNA"）。可用于扩增反应和Southern印迹实验，也可存档（参见" Efficient downstream PCR analysis"）。
DNA在单管中通过离心操作，从0.1 – 20 ml（血液）、0.1 – 2 ml（白膜层）或1 – 2 x 106个细胞（培养细胞）中纯化DNA。1小时内处理12个样本。依据样本大小，再水合体积0.2 – 1 ml。
使用FlexiGene系统从不同类型的样本中纯化获得的DNA产量 样品 体积 DNA 产量（µg） 血液* 0.3 ml 11–14 血液* 2.0 ml 75–90 血液* 10.0 ml 380–460 白膜层 0.5 ml 110–130 HeLa细胞 106 cells 25–35 * 全血以正常的白细胞计数（7x106 个细胞/ml）。
| 样品 | 体积 | DNA 产量（µg） |
|---|---|---|
| 血液* | 0.3 ml | 11–14 |
| 血液* | 2.0 ml | 75–90 |
| 血液* | 10.0 ml | 380–460 |
| 白膜层 | 0.5 ml | 110–130 |
| HeLa细胞 | 106 cells | 25–35 |
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原理
FlexiGene DNA体系可从不同量的全血、白膜层和培养细胞中纯化基因组DNA。纯化过程在单管中进行，费用少，操作简单。纯化的DNA在多种下游应用中表现良好。
程序
首先在样本中加入裂解液（参见" FlexiGene DNA procedure"）。细胞核和线粒体离心沉于底部，使用包含QIAGEN Protease的变性缓冲液重新悬浮。然后蛋白裂解，加入异丙醇沉淀DNA，离心处理，70%乙醇洗涤和干燥。使用水合缓冲液重新悬浮的DNA可即用于下游分析或–20°C贮存。可从全血、白膜层和培养细胞中获得高产量的DNA。
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应用
FlexiGene DNA Kit纯化的DNA可用于多种下游应用，包括：
- PCR
- Southern印迹
- 存档
辅助数据和图表
FlexiGene DNA procedure.
The first step is addition of lysis buffer to the samples. Cell nuclei and mitochondria are pelleted by centrifugation and resuspended in denaturation buffer containing QIAGEN Protease. Following protein digestion, DNA is precipitated by addition of isopropanol, recovered by centrifugation, washed in 70% ethanol, and dried. DNA is resuspended in hydration buffer and is ready for direct use in downstream assays or storage at –20ºC.
Specifications
| Features | Specifications |
|---|---|
| ApplicationsZH | PCR techniques, restriction digest, Southern blotting, sequencing |
| Sample amount | 100 µl - 20 ml |
| Format | Scalable |
| Time per run or per prep | 25 - 45 minutes (+ DNA rehydration) |
| Main sample type | Whole blood, buffy coat, cultured cells |
| Technology | Single tube precipitation method |
| Processing | Manual |
| Yield | 35 µg / ml whole blood |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Genomic DNA |
| Elution volume | 200 µl - 1000 µl |
资源
试剂盒操作手册 (1)
补充实验方案 (1)
其他资源 (1)
安全数据表 (1)
Certificates of Analysis (1)
Publications
The (TAAAA)n microsatellite polymorphism in the SHBG gene influences serum SHBG levels in women with polycystic ovary syndrome.
Hum Reprod; 2006; 22 (4):1031-6 2006 Dec 22 PMID:17189294
A functional SNP of interferon-gamma gene is important for interferon-alpha-induced and spontaneous recovery from hepatitis C virus infection.
Proc Natl Acad Sci U S A; 2007; 104 (3):985-90 2007 Jan 10 PMID:17215375
Lipopolysaccharide-activated CD4+CD25+ T regulatory cells inhibit neutrophil function and promote their apoptosis and death.
J Immunol; 2006; 177 (10):7155-63 2006 Nov 15 PMID:17082633
Predictors of vitamin B6 and folate concentrations in older persons: the InCHIANTI study.
Clin Chem; 2006; 52 (7):1318-24 2006 May 11 PMID:16690736
Past adult lead exposure is linked to neurodegeneration measured by brain MRI.
Neurology; 2006; 66 (10):1476-84 2006 May 23 PMID:16717205
FAQ
Do you have a protocol for purification of DNA from clotted blood using the FlexiGene DNA Kit?
Do you have stability data for genomic DNA isolated with the Flexigene DNA Kit?
Do you have a protocol for the isolation of genomic DNA from frozen clotted blood?
What is the composition of Buffer FG3?