MagAttract 96 DNA Plant Core Kit

高效、高通量的手动或自动纯化植物组织中细胞总DNA

S_1084_5_GEN_V2

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MagAttract 96 DNA Plant Core Kit (24)

Cat. No. / ID:  67163

MagAttract Suspension and Buffers for 24 x 96 preps
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US$5,032.00
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KitBuffer
MagAttract 96 DNA Plant Core Kit
Buffer RPW
MagAttract 96 DNA Plant Core Kit 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • 经济的方法
  • 产量和纯度重复性好,即用型DNA
  • 简单,快速和可靠的流程
  • 手动方法进行初次评估

Product Details

MagAttract 96 DNA Plant Core Kit结合了硅胶技术的高效性和磁珠方法的操作方便性,可选择手动、半自动,或者96孔板全自动DNA纯化。

Performance

使用MagAttract 96 DNA Plant Core Kit的纯化方案
规格: 96孔板
样本来源: 植物组织
植物原料,新鲜或者冷冻: 10–100 mg 
植物原料,风干或者冻干: 10–30 mg
常规产量: 1–15 µg
洗脱体积: 100 µl

纯化的DNA已成功用于PCR和real-time PCR(参见" Successful PCR analysis"和" Reliable real-time PCR analysis")。

See figures

Principle

MagAttract 96 DNA Plant Kit是专为全自动化、高通量从植物组织中提取基因组和叶绿体DNA而设计的。经认证的MagAttract磁粒技术结合BioRobot Plant Science(已停产)提供有效而且可重复的DNA纯化(参见" Reproducible yields")。
See figures

Procedure

植物样品使用TissueLyser等匀浆器进行机械破碎。由此产生的粉末悬浮于裂解液,充分混匀,离心。将裂解液转移到含有MagAttract磁珠的96微孔板上,基因组DNA选择性结合到磁珠表面(参见" MagAttract 96 DNA Plant Core procedure")。洗涤后,用低盐缓冲液洗脱得到纯化的基因组DNA,可即用于下游应用。MagAttract 96 DNA Plant Kit是专为使用BioRobot Plant Science系统的全自动DNA纯化而设计。QIAGEN还提供使用BioRobot RapidPlate工作站的自动化方案,和以评估为目的的手动实验方案。
See figures

Applications

MagAttract 96 DNA Plant Kit适用于从植物样本中纯化高产量DNA。纯化的DNA是可达40 kb,其中多数为23 kb的片段,可即用于多种下游应用,包括:

  • PCR
  • Real-time PCR
  • 微卫星分析
  • SNP基因分型

Supporting data and figures

Resources

试剂盒操作手册 (1)
For efficient high-throughput isolation of DNA from plant tissue 
安全数据表 (2)
Download Safety Data Sheets for QIAGEN product components.
Download Safety Data Sheets for QIAGEN product components.
Certificates of Analysis (1)
Kit Handbooks (1)
For efficient high-throughput isolation of DNA from plant tissue 
Safety Data Sheets (2)
Download Safety Data Sheets for QIAGEN product components.
Download Safety Data Sheets for QIAGEN product components.