AllPrep RNA/Protein Kit
从细胞中同时纯化RNA和蛋白质
从细胞中同时纯化RNA和蛋白质
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Cat. No. / ID: 80404
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AllPrep RNA/Protein Kit可以从同一个细胞培养样本中同时纯化总RNA和天然蛋白。
AllPrep RNA/Protein Kit适用于从同一个细胞培养样本中同时纯化总RNA和天然蛋白。该试剂盒可进行高效的样本制备,使用较少量的细胞即可进行纯化,因此适用于在mRNA和蛋白质水平评估siRNA引起的基因敲除。此外,因为RNA和蛋白质来自同一样本,所以基因敲除分析更加可靠。AllPrep RNA/Protein Kit是QIAGEN RNAi和基因表达分析产品线的一部分,可获得具有高度可重复性且灵敏的结果。该试剂盒包含优化的PCR预混液、即用型试剂、siRNA和转染试剂。该产品使RNAi和基因表达分析工作流程标准化,在生物医学研究的各个领域取得了成功。
AllPrep RNA/Protein Kit适用于从同一个细胞培养样本中同时纯化总RNA和天然蛋白。纯化得到的RNA和蛋白质在下游应用中表现出色,如real-time RT-PCR、Western印迹和酶分析。(注:待测蛋白的功能需要根据其特性进行检测)。
Total RNA was purified from HeLa cells and analyzed on the Agilent 2100 Bioanalyzer. The rRNA ratio (28S/18S) was 2.04. Total protein was purified from HeLa cells and analyzed by western blot. Analysis of duplicate protein samples with antibodies against Lamin (numclear protein) and TIM23 (mitochondrial protein) demonstrates that the total protein fraction contains proteins from subcellular compartments.
Features | Specifications |
---|---|
ApplicationsZH | PCR, real-time PCR, blotting, microarray |
Elution volume | 30–50 µl |
Format | Spin column |
Processing | Manual (centrifugation) |
Technology | Silica technology |
Time per run or per prep | 40 minutes |
Sample amount | 700 µl |
Main sample type | Cultured cells |
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | RNA, native protein |