QIAamp UltraSens Virus Kit

从血清和血浆中浓缩和纯化病毒DNA和RNA

S_1423_RPA_QA0894

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的(再)订购

QIAamp UltraSens Virus Kit (50)

Cat. No. / ID:   53704

For 50 viral nucleic acid preps: 50 QIAamp Mini Spin Columns, Proteinase K, carrier RNA, Collection Tubes (2 ml), buffers
US$678.00
登录 要查看您的账户定价。
Preparations
50
250
QIAamp UltraSens Virus Kit 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的(再)订购

特点

  • 快速纯化高品质的即用型病毒DNA和RNA
  • 无需有机抽提或乙醇沉淀
  • 重复性好,产量高
  • 完全去除污染物和抑制剂

产品详情

QIAamp UltraSens Virus Kit使用创新技术浓缩血清和血浆样品中的病毒核酸,接着使用经验证的QIAamp技术纯化核酸。样本起始体积可多至1 ml,向样本中添加创新型试剂纯化得到高浓缩核酸。试剂与核酸结合成复合物,可通过低速离心高度浓缩。

绩效

QIAamp UltraSens Virus Kit适用于从多种病毒样本中纯化病毒核酸,也可高效分离血浆和血清中的胞外基因组DNA。制备的核酸品质卓越,适用于敏感的下游应用(参见" High performance in RT-PCR")。

纯化的DNA和RNA多种下游应用,包括:

  • PCR和real-time PCR
  • 传染性疾病研究
查看图表

原理

DNA特异性结合到QIAamp硅胶膜上,污染物流出。通过两步高效洗涤完全去除二价阳离子和蛋白质等PCR抑制物,再用水或试剂盒中的缓冲液洗脱结合在离心柱上的纯DNA。

QIAamp技术从无细胞体液中纯化的病毒RNA和DNA,可即时用于PCR和印迹实验中。QIAamp样本制备技术完全得到验证认可。

程序

核酸浓缩通过将一种新型的试剂加入样本中来实现。试剂与核酸形成复合物,通过低速离心得到高浓缩核酸。该步骤可简便地对大体积样本进行核酸纯化。接着使用QIAamp硅胶膜技术纯化病毒核酸(参见" Procedure")。将裂解物上样到QIAamp离心柱,用洗涤缓冲液去除杂质,最后用纯水或低盐缓冲液洗脱得到高纯度的即用型DNA。

该流程无需超速离心或特殊的实验装置,在流程开始时进行内部控制,从而对纯化处理进行全程监测。

查看图表

应用

QIAamp UltraSens Virus Kit采用创新技术浓缩血浆和血清样本中的病毒核酸,并使用经验证的QIAamp技术纯化核酸。该流程可提高灵敏度,适用于病毒载量监测等要求较高病毒核酸回收率的应用。1小时内即可完成对1 ml血浆或血清样本的制备,核酸回收率为85%,洗脱体积为60 µl。

辅助数据和图表

Specifications

FeaturesSpecifications
ApplicationsZHPCR, qPCR, real-time PCR
Time per run or per prep1 hour
FormatMinElute columns
Elution volume60 µl
ProcessingManual (centrifugation)
Sample amount1 ml
TechnologySilica technology
Purification of total RNA, miRNA, poly A+ mRNA, DNA or proteinViral DNA, viral RNA
Main sample typeSerum, plasma
Yield>85% recovery

资源

试剂盒操作手册 (2)
For highly efficient purification of viral RNA and DNA from 1 ml plasma and serum samples
安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
快速启动实验方案 (1)
产品介绍与指南 (2)
Certificates of Analysis (1)

FAQ

What is the composition of Buffer AC in the QIAamp UltraSens Virus Kit?

The exact composition of Buffer AC is confidential. This buffer is a proprietary component of QIAamp UltraSens Virus Kits. We do not sell Buffer AC separately.

FAQ ID - 3429
What is the difference between QIAGEN Protease and QIAGEN Proteinase K provided in various QIAamp Kits?

QIAGEN Proteinase K is a subtilisin-type protease, which cleaves at the carboxyl side of hydrophobic, aliphatic and aromatic amino acids. It is particularly suitable for short digestion times. It possesses a high specific activity over a wide range of temperatures and pH values with substantially increased activity at higher temperature. Soluble calcium is not essential for enzymatic activity. This means that EDTA, which is used to inhibit Mg2+-dependent enzymes such as nucleases, will not inhibit Proteinase K activity.

QIAGEN Protease is a broad-specificity Serine protease with high activity, cleaving preferentially at neutral and acidic residues. It is an economical alternative to Proteinase K for isolation of native DNA and RNA from a variety of samples.

For more information on activity of QIAGEN Protease and Proteinase K in various buffers please click here.

FAQ ID -761
What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

FAQ ID -728
Is it possible to isolate free genomic DNA using the QIAamp UltraSens Virus Kit?
Yes, it is possible to isolate free genomic DNA from plasma and serum using the QIAamp UltraSens Virus Kit.
FAQ ID -358
What has to be done to an RNA sample before loading it onto an Agilent Bioanalyzer?

For RNA isolated on the BioRobot EZ1 and BioRobot M48:

The RNA can be directly applied to the Agilent Bioanalyzer, since it is being denatured in the final protocol steps of these isolation procedures.

For RNA prepared with all other QIAGEN RNA Isolation Products:

We recommend to denature the samples in a water bath for 2 min at 70°C, and then place them directly on ice prior to loading them onto the Agilent Bioanalyzer.

 

FAQ ID -528
How do I safely inactivate biohazardous flow-through material?

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ ID -12