QIAamp Virus BioRobot 9604 Kit

在BioRobot 9604工作站上,从无细胞体液中自动纯化病毒DNA和RNA

Products

QIAamp Virus BioRobot 9604 Kit 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。
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QIAamp Virus BioRobot 9604 Kit (12)

Cat. No. / ID:   965662

For 12 x 96 nucleic acid preps: 12 QIAamp 96 Plates, RNase-free buffers, QIAGEN Protease, AirPore Tape Sheets, Tape Pad, S-Blocks, Racks with Collection Microtubes (1.2 ml), carrier RNA, caps
US$7,005.00

特点

  • 快速纯化高品质的即用型病毒DNA和RNA
  • 无需有机提取或乙醇沉淀
  • 重复性好,产量高
  • 完全去除污染物和抑制剂

产品详情

QIAamp Virus BioRobot 9604 Kit使用经验证的QIAamp技术在BioRobot 9604工作站上自动纯化病毒核酸。全程自动化运行无需手动操作,2.5小时内即可处理96个样本,其间完成条形码读取和过程存档。

绩效

QIAamp 96孔板可确保RNA和DNA回收的孔间一致性,能可靠用于下游应用。使用QIAamp技术从无细胞体液中制备的病毒RNA和DNA可即时用于PCR和印迹实验。

原理

该流程无需苯酚-氯仿抽提。核酸特异性结合到QIAamp硅胶膜上,污染物流出。通过高效洗涤完全去除二价阳离子和蛋白质等PCR抑制剂,再用水或试剂盒中的缓冲液洗脱结合在离心柱上的核酸。

程序

试剂盒提供优化的缓冲液高效裂解样本,稳定核酸并促进其选择性结合到QIAamp硅胶膜上。裂解液中添加乙醇并上样到QIAamp 96孔板。在BioRobot 9604工作站上使用洗涤缓冲液去除杂质,最后用低盐缓冲液洗脱得到即时可用的核酸(参见" Protocol")。

该试剂盒2.5个小时内即可从96个无细胞体液样本中纯化得到高纯度的RNA和DNA,适用于从人和动物病毒中分离核酸。QIAamp样本制备技术完全得到验证认可。

查看图表

应用

QIAamp Virus BioRobot 9604 Kit使用经验证的QIAamp技术在BioRobot 9604工作站上,以96孔板规格自动化高通量纯化病毒核酸。该试剂盒可从200 µl或更少的样本中纯化核酸,包括:
  • 血浆
  • 血清
  • 脑脊液
  • 其他无细胞体液
  • 细胞培养上清液

辅助数据和图表

Specifications

FeaturesSpecifications
ApplicationsZHPCR, blotting
For automated processingBioRobot 9604 Workstation
Main sample typeSerum, plasma
Elution volume50 µl
Format96-well plates
ProcessingAutomated
Purification of total RNA, miRNA, poly A+ mRNA, DNA or proteinViral DNA, viral RNA
TechnologySilica technology
Sample amount<200 µl
Time per run or per prep<2.5 hours
Yield>90% recovery

资源

试剂盒操作手册 (1)
For simultaneous purification of viral RNA and DNA from plasma, serum, cell-culture supernatants, and cell-free body fluids using the BioRobot 9604 workstation
安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
Certificates of Analysis (1)

FAQ

What is the difference between QIAGEN Protease and QIAGEN Proteinase K provided in various QIAamp Kits?

QIAGEN Proteinase K is a subtilisin-type protease, which cleaves at the carboxyl side of hydrophobic, aliphatic and aromatic amino acids. It is particularly suitable for short digestion times. It possesses a high specific activity over a wide range of temperatures and pH values with substantially increased activity at higher temperature. Soluble calcium is not essential for enzymatic activity. This means that EDTA, which is used to inhibit Mg2+-dependent enzymes such as nucleases, will not inhibit Proteinase K activity.

QIAGEN Protease is a broad-specificity Serine protease with high activity, cleaving preferentially at neutral and acidic residues. It is an economical alternative to Proteinase K for isolation of native DNA and RNA from a variety of samples.

For more information on activity of QIAGEN Protease and Proteinase K in various buffers please click here.

FAQ ID -761
What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

FAQ ID -728
How do I safely inactivate biohazardous flow-through material?

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ ID -12