QIAGEN Protease and Proteinase K

For protease digestion during DNA and RNA preparation

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QIAGEN Protease (30 AU)

Cat. No. / ID: 19157

4 x 7.5 Anson units per vial (lyophilized)

US$365.00

EnzymeSolution

QIAGEN Protease

QIAGEN Proteinase K

QIAGEN Protease Solvent

Contains

30 (4 x 7.5) AU

7.5 AU

Quantity

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Contact an OEM expert

Features

For protease digestion in DNA and RNA isolation procedures
Fully compatible with selected QIAGEN protocols
Both enzymes quality-guaranteed by QIAGEN

Product Details

QIAGEN Protease and QIAGEN Proteinase K offer broad substrate specificity with high activity for a wide range of reaction conditions. Both proteases offer high activity in buffers commonly used in most DNA and RNA isolation procedures and are quality-guaranteed by QIAGEN.

Performance

QIAGEN Protease and QIAGEN Proteinase K offer broad substrate specificity with high activity in buffers commonly used in most DNA and RNA isolation procedures as well as in a wide range of salt, denaturant, and detergent (see table "Protease activity in commonly used buffers"), pH, and temperature conditions. Both enzymes are quality-guaranteed by QIAGEN.

Protease activity in commonly used buffers*

Buffer	Activity (%)
Buffer	QIAGEN Protease	QIAGEN Proteinase K
30 mM Tris·Cl	100	100
30 mM Tris·Cl; 30 mM EDTA; 5% Tween 20; 0.5% Triton X-100; 800 mM GuHCl†	210	313
36 mM Tris·Cl; 36 mM EDTA; 5% Tween 20; 0.36% Triton X-100; 735 mM GuHCl‡	205	301
10 mM Tris·Cl; 25 mM EDTA; 100 mM NaCl; 0.5% SDS	78	128
10 mM Tris·Cl; 100 mM EDTA; 20 mM NaCl; 1% Sarkosyl	101	74
10 mM Tris·Cl; 50 mM KCl; 1.5 mM MgCl2; 0.45% Tween 20; 0.5% Triton X-100	159	106
10 mM Tris·Cl; 100 mM EDTA; 0.5% SDS	98	120
30 mM Tris·Cl; 10 mM EDTA; 1% SDS	38	203

* pH 8.0, 50°C, 1.25 µg/ml protease, 15 min incubation.
† Buffer G2 used in QIAGEN Genomic-tip procedures for DNA isolation from blood, cell cultures, and tissue.
‡ Recommended buffer conditions for protease digestion (Buffers B1+ B2) in the QIAGEN Genomic-tip protocol for bacterial DNA isolation.

Principle

Technical specifications

	QIAGEN Protease	QIAGEN Proteinase K
Format	Lyophilized powder	Ready-to-use solution
Amount	7.5 AU or 4 x 7.5 AU	2 ml or 10 ml (20 mg/ml)
Activity	45 mAU/mg protein	>600 mAU/ml
Unit definition	One mAU is the activity that releases folin-positive amino acids and peptides corresponding to 1 µmol tyrosine per minute

QIAGEN Proteinase K is a subtilisin-type protease isolated from the saprophytic fungus Tritirachium album and is particularly suitable for short digestion times. It possesses a high specific activity that remains stable over a wide range of temperatures and pH values with substantially increased activity at a higher temperature. Soluble calcium is not essential for enzymatic activity. This means that EDTA, which is used to inhibit Mg2+-dependent enzymes such as nucleases, will not inhibit Proteinase K activity. Proteinase K is supplied in the following QIAGEN kits:

QIAamp DNA Mini Kit
QIAamp Fast DNA Stool Mini Kit
QIAamp 96 DNA Swab BioRobot Kit
DNeasy Blood & Tissue Kit
DNeasy 96 Blood & Tissue Kit
QIAamp DNA Micro Kit
QIAamp MinElute Media Kit
QIAamp UltraSens Virus Kit
QIAamp Media MDx Kit

QIAGEN Protease is a serine protease isolated from a recombinant Bacillus strain. It is an economical alternative to Proteinase K for isolation of native DNA and RNA from a variety of sources. QIAGEN Protease is completely free of DNase and RNase activities. QIAGEN Protease is supplied in the following QIAGEN kits:

QIAamp DNA Blood Mini, Midi and Maxi Kits
QIAamp 96 DNA Blood Kit
Blood & Cell Culture DNA Kits
QIAamp DNA Blood BioRobot 9604 Kit
QIAamp Virus BioRobot 9604 Kit
QIAamp DNA Blood BioRobot MDx Kit
QIAamp DSP Virus Kit
QIAamp DSP DNA Blood Mini Kit

Note: Users of manual QIAamp DNA Blood Kits and QIAGEN Blood & Cell Culture DNA Kits should resuspend each bottle of QIAGEN Protease with 7 ml distilled water.

Note: Users of the QIAamp DNA Blood BioRobot 9604 Kit should resuspend each bottle of QIAGEN Protease with 10 ml distilled water.

Note: QIAGEN Protease is not compatible with Buffer ATL in the DNeasy Tissue, DNeasy 96 Tissue and QIAamp DNA Mini Kit. In the presence of >0.5% SDS, >1% sarkosyl or high concentrations of other detergents, the EDTA concentration must be <8 mM for full activity over extended incubation times.

Procedure

Instructions for using QIAGEN Protease or QIAGEN Proteinase K are provided in the corresponding kit handbook.

Applications

QIAGEN Protease and QIAGEN Proteinase K provide protease digestion during DNA and RNA preparation. Subtle differences between the enzymes should be considered when planning protease digestions.

Resources

Download Safety Data Sheets for QIAGEN product components.

FAQ

QIAGEN Proteinase K is a subtilisin-type protease, which cleaves at the carboxyl side of hydrophobic, aliphatic and aromatic amino acids. It is particularly suitable for short digestion times. It possesses a high specific activity over a wide range of temperatures and pH values with substantially increased activity at higher temperature. Soluble calcium is not essential for enzymatic activity. This means that EDTA, which is used to inhibit Mg2+-dependent enzymes such as nucleases, will not inhibit Proteinase K activity.

QIAGEN Protease is a broad-specificity Serine protease with high activity, cleaving preferentially at neutral and acidic residues. It is an economical alternative to Proteinase K for isolation of native DNA and RNA from a variety of samples.

For more information on activity of QIAGEN Protease and Proteinase K in various buffers please click here.

FAQ ID -761

QIAGEN Proteinase K is stable for up to 1 year after delivery when stored at room temperature. To prolong the shelf-life of Proteinase K, storage at 2–8°C is recommended.

FAQ ID - 3447

QIAGEN Protease is inactivated by incubation at 70°C for 15 minutes.

To our knowledge, Proteinase K cannot be completely heat-inactivated. Even when incubating at 95°C for 10 minutes, some enzymatic activity remains. This will not negatively affect the QIAamp Procedure, since the enzyme will be efficiently removed by the wash steps in the protocols.

FAQ ID -315

No, QIAGEN Protease is not compatible with Buffer ATL.