QIAshredder

简单快速匀浆细胞和组织的裂解液

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QIAshredder (250)

Cat. No. / ID: 79656

250 disposable cell-lysate homogenizers for use in nucleic acid minipreps, caps

US$515.00

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Pieces

250

数量

QIAshredder 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的（再）订购

特点

代替针筒抽吸的匀浆步骤
可以减少样品的消耗
防止样本间的交叉污染
可以去除不溶性沉淀碎片和降低粘稠度

产品详情

QIAshredder的离心柱中含有独特的生物聚合物剪切体系。将细胞或组织裂解物上样到置于收集管中的QIAshredder并进行离心，从而收集到匀浆的裂解物。一般来说，所得RNA的产量与质量与转子-定子匀浆方法相当（参见"Comparison of RNA yields"）。

辅助数据和图表

Comparison of RNA yields.

资源

RNeasy Mini Kit - For purification of total RNA from animal cells, animal tissues, bacteria, and yeast, and for RNA cleanup; RNeasy Protect Mini Kit - For immediate stabilization of RNA in harvested animal tissues and subsequent total RNA purification; RNeasy Plant Mini Kit - For purification of total RNA from plants and filamentous fungi

Publications

The protective role of a small GTPase RhoE against UVB-induced DNA damage in keratinocytes.

Boswell SA; Ongusaha PP; Nghiem P; Lee SW;

J Biol Chem; 2006; 282 (7):4850-4858 2006 Dec 14 PMID:17170105

Cell specific patterns of methylation in the human placenta.

Grigoriu A; Ferreira JC; Choufani S; Baczyk D; Kingdom J; Weksberg R;

Epigenetics; 2011; 6 (3):368-79 2011 Mar 1 PMID:21131778

Mechanisms of confluence-dependent expression of CD26 in colon cancer cell lines.

Abe M; Havre PA; Urasaki Y; Ohnuma K; Morimoto C; Dang LH; Dang NH;

BMC Cancer; 2011; 11 :51 2011 Feb 1 PMID:21284881

Effects of alpha-calcitonin gene-related peptide on osteoprotegerin and receptor activator of nuclear factor-κB ligand expression in MG-63 osteoblast-like cells exposed to polyethylene particles.

Xu J; Kauther MD; Hartl J; Wedemeyer C; Study was performed at the University of Duisburg - Essen, Germany;

J Orthop Surg Res; 2010; 5 :83 2010 Nov 4 PMID:21050480

Alpha-calcitonin gene-related peptide can reverse the catabolic influence of UHMWPE particles on RANKL expression in primary human osteoblasts.

Kauther MD; Xu J; Wedemeyer C;

Int J Biol Sci; 2010; 6 (6):525-36 2010 Sep 15 PMID:20877694

FAQ

Yield and integrity of genomic DNA depend on the disruption and homogenization method used with the Allprep DNA/RNA Kits.

Homogenization with the TissueRuptor (or other rotor–stator homogenizer, such as the Polytron) or the TissueLyser results in greater DNA fragmentation (depending on homogenization time and intensity). However, shorter DNA fragments are easier to elute, so DNA yields will be higher.

In contast, homogenization using the QIAshredder (or a syringe and needle) is more gentle, resulting in less DNA fragmentation. However, longer DNA fragments are more difficult to elute, so DNA yields may be lower.

FAQ ID -1751

The QIAshredder is a unique biopolymer shredding system in a microcentrifuge spin-column format. It homogenizes cell or tissue lysates to reduce viscosity. Homogenization shears the high-molecular weight genomic DNA and other high-molecular-weight cellular components to create a homogenous lysate. The QIAshredder is chemically inert and will not bind nucleic acids.

It cannot replace tissue disruption or enzymatic cell wall lysis by mechanical and chemical methods, respectively. Both, efficient cell wall disruption and lysate homogenization are fundamental for successful RNA isolation from all types of samples. Once complete disruption has been achieved, the QIAshredder Homogenizer can be used in place of needle and syringe, or rotor-stator homogenization.

For further information regarding sample disruption and homogenization please refer to the 'Disruption and homogenization of starting materials' section in the RNeasy Mini Handbook, and see FAQ 139.

FAQ ID -631

The QIAshredder Maxi spin columns from the DNeasy Plant Maxi Kit are not recommended for use in conjunction with QIAGEN's RNA isolation products. The filter can only tolerate low centrifugal forces not sufficient to shear genomic DNA. If genomic DNA is not sheared, there will be an increase in genomic DNA contamination in the purified RNA.

For an alternative to QIAshredder homogenization in combination with RNeasy Midi/Maxi Preps, please see FAQ 560.

FAQ ID -616

We only offer QIAshredder spin columns for use with the RNeasy Mini Kit. When working with RNeasy Midi/Maxi, we recommend using a rotor-stator, or mortar and pestle with needle and syringe homogenization.

FAQ ID -560

Yes

FAQ ID -501