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特点
- 从多种类型的食物样本中纯化DNA
- 纯化复杂食品样本的DNA时不残留PCR抑制剂
- 加速的CTAB实验流程，实现高效的DNA分离
- 简便、可靠的自动处理，节省时间和费用
产品详情
DNeasy mericon 96 QIAcube HT Kit可在QIAcube HT仪器上，从多种食品原料或加工食品中便利的自动纯化DNA。该试剂盒采用成熟的DNeasy硅胶模96孔板纯化技术，能够去除复杂的食品样本中的污染物和抑制剂，获得的高品质DNA可直接用于下游应用。该试剂盒是QIAGEN食品检测产品线的一部分，QIAGEN还提供病原体DNA检测、转基因生物DNA检测和成分鉴定试剂盒。
绩效
DNeasy mericon 96 QIAcube HT Kit能够从多种食品原料和加工食品中快速自动纯化DNA，并去除复杂食品样本中的PCR抑制剂残留。DNeasy mericon纯化试剂盒为适用范围广范的DNA分离方法，即使处理PCR抑制剂含量高、经过深加工的、多脂、酸性、高或低DNA含量的食品，也能获得出色、可靠的结果（参见 High yields of DNA and minimal inhibitor carryover）。QIAcube HT和专用的DNeasy mericon 96 QIAcube HT Kit搭配使用，能够从食品样本中中高通量自动纯化DNA。现在您可以通过快速、便利的自动流程，纯化获得与手工试剂盒相同质量的高品质的DNA（参见 Purification of DNA from food samples）。
查看图表
原理
DNeasy mericon 96 QIAcube HT Kit在QIAcube HT仪器上，能够从最多300 mg食品样本中自动纯化核酸。纯化的高品质DNA能够在PCR等下游应用中获得出色结果。
DNeasy mericon 96 QIAcube HT Kit结合了硅胶模的选择性结合特性和96孔板的高通量优点，可在QIAcube HT仪器上全自动同时处理24–96个样本。
DNeasy mericon 96 QIAcube HT Kit结合了硅胶模的选择性结合特性和96孔板的高通量优点，可在QIAcube HT仪器上全自动同时处理24–96个样本。
| 规格 | 描述 |
|---|---|
| 样本数量 | 24–96个样本（每批8个） |
| 样本起始体积 | 300 mg食品材料 |
| 洗脱体积 | 200 μl |
| 时间长度 | 大约73分钟内处理96个样本 大约42分钟内处理24个样本 |
程序
DNeasy mericon 96 QIAcube HT Kit采用改良的十六烷基三甲基溴化铵（CTAB）核酸提取技术，能够在QIAcube HT仪器上从食品材料中快速自动纯化DNA（参见 Efficient and rapid automated DNA extraction）。匀浆的食品材料经过手动预处理，使用Food Lysis Buffer和蛋白酶K进行高效裂解。氯仿萃取能够去除CTAB-protein、CTAB-细胞碎片或CTAB-多糖复合物。将含有DNA的水相移液至96孔的S-Block。后续的纯化步骤可在QIAcube HT上自动完成。
QIAcube HT在水相中加入Buffer PB，让后将混合液移至DNeasy 96孔板。通过真空操作，核酸结合至硅胶模，污染物流出。洗涤步骤洗去残留的污染物和抑制剂，之后用Buffer EBA洗脱DNA。获得的纯DNA可及用于下游的mericon real-time PCR分析。
QIAcube HT在水相中加入Buffer PB，让后将混合液移至DNeasy 96孔板。通过真空操作，核酸结合至硅胶模，污染物流出。洗涤步骤洗去残留的污染物和抑制剂，之后用Buffer EBA洗脱DNA。获得的纯DNA可及用于下游的mericon real-time PCR分析。
查看图表
应用
DNeasy mericon 96 QIAcube HT Kit能够从食品原料或加工食品中纯化高品质DNA，可去除复杂食品材料中的PCR抑制剂。纯化的DNA可用于多种下游应用：
- 食品安全检测和质量检测
- 成分鉴定
- 检测转基因成分
- 检测可能的过敏原
辅助数据和图表
Efficient and rapid DNA extraction.
The DNeasy mericon 96 QIAcube HT Kit enables DNA extraction from food samples in radically shorter time compared to conventional CTAB protocols. Automated purification of 24–96 samples can be carried out on the QIAcube HT instrument in just a few hours with little hands-on time.
资源
实验方案软件 (5)
安全数据表 (1)
快速启动实验方案 (2)
技术资讯 (2)
试剂盒操作手册 (1)
产品介绍与指南 (1)
用户使用手册 (1)
Certificates of Analysis (1)
FAQ
Can I run the QIAcube HT with the QIAxtractor software or vice versa?
Can I upgrade a Corbett CAS-1820 to a QIAcube HT?
What file is required for reporting a QIAcube HT or QIAxtractor issue?
I am getting vacuum errors on my QIAcube HT. What should I do?
1. Check the vacuum tubing to make sure it is not pinched or obstructed in some way. Sometimes the tubing can become pinched against the bench top when the vacuum station is placed too far from the instrument.
2. Make sure that the channeling adapter is properly placed. Check if the carriage is moving the plate completely from the waste chamber position to the elution chamber position. If the calibration seems incorrect, the instrument may need to be re-taught at this position.
3. When using less than a full plate, make sure all the unused columns are covered with an adhesive seal.
Also, make sure each column of 8 samples is full. When processing a number of samples that results in < 8 samples per column, use water as a substitute in the empty positions until the column is filled. This ensures even vacuum across all unsealed well positions.
4. Make sure correct plates are used for the selected protocol.
5. When the vacuum is on, please listen for any hissing noise from the tubing that is coming down to the waste container. This could indicate leaks in the tubing.
If the issue persists, please contact QIAGEN Technical Service.
FAQ ID -9106
What do I do if the QIAcube HT or QIAxtractor software runs in virtual mode while the instrument is connected to the computer?