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What should be the concentration of my protein for the Nextal Cubic Phase products?
FAQ ID -2417
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What is the PAXgene 96 filter plate?
FAQ ID -2486
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What is the range indicated by 'Std. Error' on 'Results' page?
FAQ ID -2456
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Can the Internal Controls be ordered separately in the QuantiFast Pathogen + IC kit for use during purification?
FAQ ID -2451
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Can the PAXgene Blood RNA MDx Kit be used with both the BioRobot Universal System and the BioRobot MDx?
FAQ ID -2489
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Can the PAXgene Blood RNA MDx Kit be used for diagnostic or prognostic procedures?
FAQ ID -2488
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Can the PAXgene Blood miRNA System be used for diagnostic or prognostic procedures?
FAQ ID -2494
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Can the QIAsymphony PAXgene Blood RNA System be used for diagnostic or prognostic procedures?
FAQ ID -2496
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How are the different mutations for a single gene normalized on the qBiomarker Somatic Mutation PCR array?
FAQ ID -2428
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At what temperature can I perform the NexTal Cubic Phase experiment and incubate the plate?
FAQ ID -2415
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How can I check DNA sample quality before using the Somatic Mutation PCR Arrays & Assays?
FAQ ID -2430
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How much DNA starting sample is needed for analysis with a Somatic Mutations PCR Array? And Assay?
FAQ ID -2431
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How do I get started using the NeXtal CubicPhase kits?
FAQ ID -2413
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After bisulfite conversion, can the DNA be stored?
FAQ ID -2409
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Can I buy QIAquick and MinElute columns separately?
FAQ ID -2460
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How many samples can be processed with the QIAsymphony PAXgene Blood RNA Kit?
FAQ ID -2497
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How pure should my protein be with the NexTal CubicPhase kits?
FAQ ID -2414
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How many samples can be processed with the PAXgene Blood RNA MDx Kit?
FAQ ID -2490
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How many PAXgene Blood RNA Tubes can be processed per PAXgene 96 Blood RNA Kit well plate?
FAQ ID -2485
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In the REST software, what is the range indicated by 'Std. Error' on the 'Results' page?
FAQ ID -2455
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If less than 48/96 samples are being processed, is it possible to leave positions empty with the PAXgene Blood RNA MDx Kit?
FAQ ID -2492
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How do I determine if my gene expression results are statistically significant in the REST software?
FAQ ID -2458
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How does the DNA Protection work in the EpitTect Bisulfite kits?
FAQ ID -2410
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How is the hypothesis test performed in the REST software?
FAQ ID -2459
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How many different genes and gene mutations are on a Somatic Mutation PCR Array?
FAQ ID -2427
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How long does a QuantiFast Pathogen + IC run take on the Rotor-Gene Q?
FAQ ID -2452
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How do I know in which phase of my NeXtal CubicPhase cristallisation experiment I am?
FAQ ID -2421
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How do I harvest the crystals from meso phase? Are special tools needed?
FAQ ID -2422
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How great is the enrichment of miRNA when using the dedicated PAXgene Blood miRNA Kit compared to the PAXgene Blood RNA Kit?
FAQ ID -2495
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What are important points regarding the genomic DNA wipeout buffer in the QuantiFast Probe RT-PCR Plus Kit?
FAQ ID -2359
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Will the sequence of the QuantiFast Probe Assays be provided?
FAQ ID -2369
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Why are there various different QuantiFast Probe Assays for my gene (previous versions, transcript variants)?
FAQ ID -2366
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Why are samples centrifuged at 4°C after addition of chloroform to the QIAzol lysates in following kits: RNeasy Lipid Tissue Mini/Midi kits, RNeasy Microarray Tissue Mini kit, RNeasy Plus Universal Mini/Midi kit, RNeasy 96 Universal Tissue kit, miRNeasy Mini kit, miRNeasy Micro kit, miRNeasy 96 kit and miRNeasy Serum/Plasma kit?
FAQ ID -2345
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Why are the QuantiFast Probe Assays located on a single exon, thereby also detecting genomic DNA?
FAQ ID -2365
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What dedicated QIAcube Kits are available?
FAQ ID -2337
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What are the recommended storage conditions of the QuantiFast Probe RT-PCR Plus Kit components?
FAQ ID -2355
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What are the main differences between the qBiomarker PCR Arrays and the RT2 Profiler PCR Arrays?
FAQ ID -2438
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What are the differences between the existing QuantiFast Probe RT-PCR Kit and the new Plus Kit?
FAQ ID -2358
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What is the concentration of the primers and the probe in a reaction using the QuantiFast Probe Assays?
FAQ ID -2370
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What is the average length of a QuantiFast Probe Assay?
FAQ ID -2374
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Is the RNeasy Plus Universal Mini Kit suited for adipose, brain, and other fatty tissues?
FAQ ID -2343
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Is there a size limit to the protein I can crystallize with the NexTal Cubic Phase products?
FAQ ID -2331
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Is the T790M mutation included in the EGFR PCR kit?
FAQ ID -2314
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Is it necessary to perform calibration steps for the use of the MAX dye in duplex RT-PCR experiments on different cyclers?
FAQ ID -2373
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Is there also a miRNeasy Plus Universal Kit available?
FAQ ID -2346
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I use cultured cells as starting material. Can I also use the RNeasy Plus Universal Kit?
FAQ ID -2342
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I would like to order the QuantiFast Probe Assays without master mix. Is this possible?
FAQ ID -2360
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My ADAPT report states "Run Invalid" and does not display any sample results. Why is this?
FAQ ID -2306
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Is the yield and quality of RNA and DNA purified with the AllPrep DNA/RNA FFPE Kit comparable to yield and quality of nucleic acids purified separately with other methods.
FAQ ID -2349
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Is V600E the only mutation that can be detected with the codon 600 assay in the BRAF Pyro Kit?
FAQ ID -2382
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Which Rotor-Gene Q rotor should be used with the EGFR RGQ PCR kit?
FAQ ID -2315
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Which kit, the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit, do you recommend for transfection?
FAQ ID -2320
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Which probe labels are available for the QuantiFast Probe Assays?
FAQ ID -2361
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Which purification kits are recommended for the Pyro kits?
FAQ ID -2380
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What should be the concentration of my protein with the NexTal Cubic Phase products?
FAQ ID -2325
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What is the maximum plasmid size which I can use with the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit?
FAQ ID -2316
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What plug-ins are available for the Pyrosequencing kits?
FAQ ID -2381
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Which crystallization solutions are compatible with lipidic cubic phase crystallization?
FAQ ID -2418
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Which deparaffinization method can I use with the AllPrep DNA/RNA FFPE Kit?
FAQ ID -2352
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What species are QuantiTect Probe Assays offered for?
FAQ ID -2367
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Is it also possible to isolate miRNA with the AllPrep DNA/RNA FFPE Kit?
FAQ ID -2348
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Can the insertions in Exon 20 be detected with the EGFR Pyro Kit?
FAQ ID -2383
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Can the QuantiFast Probe Assay detect short fragmented RNA as well as longer fragments of RNA?
FAQ ID -2364
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Can the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit be used for low-copy number plasmids?
FAQ ID -2317
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Can I use the RNeasy Plus Universal Mini Kit for all types of tissue?
FAQ ID -2338
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Can negative crossing threshold (CT) values be negative when using the KRAS and EGFR PCR kits?
FAQ ID -2300
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Can QuantiFast Probe Assays be ordered offline via e-mail or fax?
FAQ ID -2368
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For the the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit, can plates be partially used?
FAQ ID -2319
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For which applications can the RNA isolated with the RNeasy Plus Universal Kit also be used?
FAQ ID -2339
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Does the KRAS PCR kit cover the codon 61 mutation?
FAQ ID -2308
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Do you have any information or guidelines regarding the choice of reference genes for real-time PCR?
FAQ ID -2371
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Are the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit suited for short hairpin vectors?
FAQ ID -2318
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Are the QuantiFast Probe Assays hydrolysis or FRET probes?
FAQ ID -2363
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Are the buffers in the RNeasy QIAcube kit delivered in QIAcube buffer bottles?
FAQ ID -2334
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Are QIAshredder spin columns delivered with the RNeasy Mini QIAcube Kit?
FAQ ID -2333
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Can I use more than 10ng DNA for the PCR reaction for the Pyro kits?
FAQ ID -2379
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Can I do singleplex PCR with the QuantiFast Probe Assays?
FAQ ID -2362
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Are the QuantiFast Probe Assays wet-lab validated?
FAQ ID -2375
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Are you able to supply a Certificate of Analysis for the KRAS and EGFR PCR kits?
FAQ ID -2301
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At what temperature can I perform the experiment with the NexTal Cubic Phase kits?
FAQ ID -2323
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How much can the mixed standard ?Ct values vary in the KRAS PCR kit?
FAQ ID -2307
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How many samples can be processed per RNeasy Mini QIAcube kit?
FAQ ID -2332
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How much DNA/RNA can be isolated with the AllPrep DNA/RNA FFPE Kit?
FAQ ID -2353
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How many freeze-thaw cycles can the master mix contained in the QuantiFast Probe RT-PCR Plus Kit tolerate?
FAQ ID -2357
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How pure should the chloroform used with the RNeasy Plus Universal Mini Kit be?
FAQ ID -2344
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How much sample material can I use for DNA/RNA purification with the AllPrep DNA/RNA FFPE Kit?
FAQ ID -2351
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How much time can be saved when using the RNeasy Mini QIAcube Kit?
FAQ ID -2335
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How does the gDNA eliminator solution of the RNeasy Plus Universal Kit work?
FAQ ID -2341
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How does the QuantiFast Probe RT-PCR Plus Kit eliminate genomic DNA contamination?
FAQ ID -2354
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How do I analyze the data for the particular iPSC qBiomarker Arrays I have ordered?
FAQ ID -2440
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How are the genes in each iPSC qBiomarker PCR Array selected?
FAQ ID -2439
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How can I adjust the ROX concentration in the QuantiFast Probe RT-PCR Plus master mix?
FAQ ID -2356
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How long can I store my purified DNA/RNA isolated with the AllPrep DNA/RNA FFPE kit?
FAQ ID -2350
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How is the RNeasy Plus Universal Kit different from the RNeasy Plus Kit?
FAQ ID -2340
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How do I harvest the crystals from meso phase? Are special tools needed with the NexTal Cubic Phase products?
FAQ ID -2330
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Is a special processing protocol needed?
FAQ ID -2523
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What is the detection limit for the Investigator Quantiplex Kit?
FAQ ID -2572
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Is it possible to archive PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue blocks?
FAQ ID -2524
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The disruption of my sample worked well with the TissueLyser LT, but I am not satisfied with the RNA yield. What do you suggest?
FAQ ID -2555
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What are the dimensions of the TissueLyser LT?
FAQ ID -2545
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On which cyclers can the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit be used?
FAQ ID -2596
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What is the average RNA yield from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -2534
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What do I have to consider if I am using Applied Biosystems SDS software version 1.2.3 to run an Investigator Quantiplex reaction?
FAQ ID -2570
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What is in the elution buffer in the PAXgene Bone Marrow RNA kit?
FAQ ID -2511
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What are the storage conditions for the mericon Assay kits?
FAQ ID -2540
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Is the performance of the HiPerFect HTS Reagent affected if it has accidentally been stored at°C?
-20
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Is it possible to embed PAXgene Tissue fixed and stabilized samples in Optimal Cutting Temperature (OCT) medium for freezing?
FAQ ID -2525
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Is it possible to microdissect PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissues?
FAQ ID -2531
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Is the morphology after H&E staining comparable to formalin-fixed samples?
FAQ ID -2526
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Is RNA from red blood cells also isolated when RNA is extracted from a PAXgene Bone Marrow RNA Tube?
FAQ ID -2509
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Is the 260/230 nm absorbance ratio specified for RNA derived from the PAXgene Bone Marrow RNA System?
FAQ ID -2514
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Which version of the Rotor-Gene Q software can be used to run the Investigator Quantiplex?
FAQ ID -2568
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What is the maximum tissue size that can be fixed in a PAXgene Tissue Container?
FAQ ID -2518
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Why do I observe a CT shift for the Internal Control in some samples in comparison to the Ct value for the Internal Control of the dilution series of the Control DNA Z1?
FAQ ID -2575
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What RNA yields are expected with the PAXgene Bone Marrow RNA System?
FAQ ID -2506
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What is the RT-PCR performance of RNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissue compared to RNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?
FAQ ID -2538
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What is the nature of the heterologous Internal Amplification Control and Positive Control in the mericon Assay kits?
FAQ ID -2543
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What is the expected amplicon size when using the mericon Assay kits?
FAQ ID -2544
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What is the longest period you recommend to leave out the dilute HiPerFect HTS at RT before use?
FAQ ID -2560
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What is the typical amount of tissue used for disruption with the TissueLyser LT?
FAQ ID -2552
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What kind of sample tubes are recommended to use with the TissueLyser LT?
FAQ ID -2553
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What is the purity of nucleic acids extracted with the PAXgene Tissue kits?
FAQ ID -2533
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Can the PAXgene Bone Marrow RNA System be used to isolate genomic DNA or viral RNA and DNA?
FAQ ID -2507
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Can the PAXgene Bone Marrow RNA System be used with animal bone marrow or with blood samples?
FAQ ID -2501
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Can the PAXgene RNA stabilization solution/bone marrow mixture from the PAXgene Bone Marrow RNA Tube be disposed of by adding bleach?
FAQ ID -2515
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Can sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for other histochemical staining techniques, such as PAS?
FAQ ID -2530
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Can the PAXgene Bone Marrow RNA System be used for other human body fluids than bone marrow samples or cultured cells of human origin?
FAQ ID -2502
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Can the sample tubes and the TissueLyser LT Adapter be cooled with liquid nitrogen?
FAQ ID -2549
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Can the TissueLyser LT Adapter be removed for cleaning?
FAQ ID -2546
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Can the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit be used with hybridization probes?
FAQ ID -2595
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Can the samples on the TissueLyser LT be protected from degradation of nucleic acids or proteins?
FAQ ID -2548
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When using HiPerFect HTS Reagent is it necessary to optimize amount of siRNA and transfection reagent?
FAQ ID -2556
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When using the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit, what should the fluorescent label of the probe be for the customer-defined assay to detect the pathogen?
FAQ ID -2594
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Which human target is used for the quantification in the Investigator Quantiplex Kit?
FAQ ID -2577
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What sample numbers can be processed on the TissueLyser LT?
FAQ ID -2547
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What size bead should I use for tougher samples with the TissueLyser LT?
FAQ ID -2554
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When performing the Internal Control assay for the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit, what channel or filter can be used to detect MAX?
FAQ ID -2597
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Why is the fluorescence signal of the Internal Control on my Rotor-Gene Q very low?
FAQ ID -2574
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Which version of the Applied Biosystems 7500 Real-Time PCR System software can be used to run the Investigator Quantiplex?
FAQ ID -2569
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Can specific types of white blood cells be enriched from a PAXgene Bone Marrow RNA Tube prior to the RNA isolation?
FAQ ID -2508
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Are there any accessories required for sample disruption with the TissueLyser LT?
FAQ ID -2551
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At what temperature should I store the Investigator Quantiplex Kit?
FAQ ID -2578
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Can bone marrow aliquots other than 2 mL be pipetted into the PAXgene Bone Marrow RNA Tube?
FAQ ID -2503
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Are the Internal Controls used in the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit homologous to a known target?
FAQ ID -2598
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Can I use water to elute the RNA in the PAXgene Bone Marrow RNA Kit?
FAQ ID -2512
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Can bone marrow from leukemia patients with elevated numbers of white blood cells be processed with the PAXgene Bone Marrow RNA System?
FAQ ID -2510
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Can PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for in situ hybridization?
FAQ ID -2529
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How long is the fixation time?
FAQ ID -2519
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Can HiPerFect HTS be used for miRNA?
FAQ ID -2561
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Can HiPerFect HTS Reagent be used for transfecting shRNAs?
FAQ ID -2559
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How long does the sample disruption with the TissueLyser LT take?
FAQ ID -2550
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How many times can the Internal Controls used in the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit be freeze-thawed?
FAQ ID -2599
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How long can I store the dilutions of the Control DNA Z1 standards at 2°C?
-8
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How long can primer and probes from the mericon Assay kits be kept at°C once reconstituted?
-20
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How should the PAXgene Bone Marrow RNA Tubes be frozen after collection and for how long?
FAQ ID -2505
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How long does an Investigator Quantiplex run take?
FAQ ID -2566
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How much time may the aspirated bone marrow sample be allowed to incubate before pipetting it into the PAXgene Bone Marrow RNA Tube?
FAQ ID -2504
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I routinely heat an aliquot of the RNA eluate prior to my RT reaction. Do I still need to heat the eluate after the PAXgene Bone Marrow RNA preparation?
FAQ ID -2513
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Do I have to calibrate new dyes on my Applied Biosystems 7500 or 7500 Fast Real-Time PCR System?
FAQ ID -2571
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Do you have FACS or Fluoro-Microscopy data using HiPerFect HTS Reagent for delivery of siRNA?
FAQ ID -2557
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How can I streamline the quantification workflow for the Investigator QuantiPlex Kit?
FAQ ID -2579
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How is stability and performance of these mericon Assay kits guaranteed?
FAQ ID -2542
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How long are the target sequences of Investigator Quantiplex?
FAQ ID -2576
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For which real-time cyclers has the Investigator Quantiplex Kit been validated?
FAQ ID -2567
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How are the supernatants resulting from the first two spin steps of the PAXgene Bone Marrow RNA kit purification procedure disposed?
FAQ ID -2516
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What is the part number for O-rings for QIAsymphony SP and AS instruments? How often should the O-rings be replaced?
FAQ ID -3055
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Where can I find the ordering information for QIAcube accessories such as Shaker Rack plugs, Rack labeling strips, Reagent bottle rack, 30mL Reagent bottles, Rotor Adapter holder, Rotor adapters, Sample tubes CB, Sample tubes RB and Spin column adapter rings?
FAQ ID -3054
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Why are two reagents used in the PAXgene Tissue System?
FAQ ID -3022
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Do you recommend using indicator cards for buccal cells on FTA with Investigator IDplex GO! Kit?
FAQ ID -3095
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Have primer sequences in the Investigator IDplex GO! Kit been changed compared to the Investigator IDplex Plus assay?
FAQ ID -3092
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Do I need to evaluate optimal PCR cycle numbers for the Investigator IDplex GO! Kit with my samples?
FAQ ID -3091
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Is it possible to increase the amount of sample to obtain higher signals using the Investigator IDplex GO! Kit?
FAQ ID -3090
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Can the Investigator IDplex GO! PCR protocol be run on different PCR endpoint cyclers?
FAQ ID -3089
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What is the safest procedure to add FTA punches to the PCR reactions of the Investigator IDplex GO! Kit?
FAQ ID -3096
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Can additional positive controls be used for the Investigator IDplex GO! Kit?
FAQ ID -3094
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Is there a need to compensate for additional liquid volume added with the positive control DNA, or a buccal swab lysate in the Investigator IDplex GO! Kit?
FAQ ID -3088
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Can the allelic ladder of the Investigator IDplex Plus Kit be used for analysis of Investigator IDplex GO! Kit?
FAQ ID -3093
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What is the maximum size of tissue that can be fixed in a PAXgene Tissue FIX Container (50 ml)?
FAQ ID -3024
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5. What is the maximum size of the tissue sample that can be fixed in a PAXgene Tissue Container?
FAQ ID -3023
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What happens if a tissue sample larger than 20 x 20 x 20 mm is used for fixation with the PAXgene Tissue FIX Container (50 ml)?
FAQ ID -3025
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Is it possible to fix over a weekend?
FAQ ID -3027
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How long is the fixation time when using PAXgene Tissue FIX Container?
FAQ ID -3026
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How long can a tissue specimen stay in the PAXgene Tissue STABILIZER?
FAQ ID -3028
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What is the minimum incubation time required when using the PAXgene Tissue STABILIZER?
FAQ ID -3029
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Is it possible to integrate the PAXgene Tissue STABILIZER into the processing?
FAQ ID -3037
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Is it possible to archive PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3038
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Is the morphology after H&E staining comparable to formalin-fixed samples?
FAQ ID -3039
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Are immunohistochemistry (IHC) assays developed for formalin-fixed, paraffin-embedded (FFPE) compatible with PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3040
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Is it possible to use formalin-fixed, paraffin-embedded (FFPE) kits and protocols for extraction of biomolecules from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3043
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is the purity of nucleic acids isolated with the PAXgene Tissue Kits?
FAQ ID -3044
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What is the average RNA yield from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3045
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What is the integrity of RNA from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3046
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What is the RT-PCR performance of RNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue compared to RNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?
FAQ ID -3050
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How can proteins purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be analyzed?
FAQ ID -3052
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What is the PCR performance of DNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue compared to DNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?
FAQ ID -3051
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What is the DNA integrity from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3047
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Does the PAXgene Tissue RNA Kit co-purify small RNAs?
FAQ ID -3048
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How can I extract proteins from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3049
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Can PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for in situ hybridization?
FAQ ID -3042
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What tubes can be used with TissueLyser Adapter Set 2 x 96 (cat. no. 69984)?
FAQ ID -3086
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What is the concentration of the Gentra RNase A that is used with the Autopure and Puregene protocols?
FAQ ID -3083
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What are the typical yields obtained from Buccal swabs on BioSprint 96?
FAQ ID -3071
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Where can I find a copy of the .kpf file for a BioSprint protocol?
FAQ ID -3073
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Can the DyeEx 96 kit be used to clean sequencing reactions to be run on ABI 3730?
FAQ ID -3075
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Can the FastLane RT reaction be incubated longer than 30 min at 42 deg C?
FAQ ID -3079
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Does carrier RNA interfere with the QuantiTect Whole Transcriptome amplification?
FAQ ID -3080
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What are the advantages and disadvantages of turning on the tip “re-use” feature on the QIAgility?
FAQ ID -3064
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How precise is the QIAgility?
FAQ ID -3063
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How long does it take to set up a 96-well plate PCR reaction on the QIAgility?
FAQ ID -3066
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Can I use a bar code scanner or reader on the QIAgility?
FAQ ID -3067
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Can I use a USB drive to transfer protocols to BioSprint?
FAQ ID -3072
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Can BioSprint 96 be connected to LIMS?
FAQ ID -3070
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How can I find the run parameters for a BioSprint protocol that I am running?
FAQ ID -3069
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Can the FastLane Cell lysates (containing stabilized RNA, step 8. in Fastlane protocol) be stored?
FAQ ID -3077
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Can I use specific primers with the FastLane Kits?
FAQ ID -3078
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What is the expected level of endotoxins in plasmid DNA purified with QIAGEN Plasmid kits, QIAfilter Plasmid kits and HiSpeed Plasmid purification kits?
FAQ ID -3082
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What is the expected level of endotoxins in plasmid DNA purified with QIAprep spin miniprep kit?
FAQ ID -3081
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What is the concentration of the different Proteinase K solutions sold by QIAGEN?
FAQ ID -3084
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Is the Fast Reaction Mix ready to use in the Investigator IDplex GO?
FAQ ID -3087
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Where can I access Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit?
FAQ ID -3085
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Is it possible to use a standard processor — the kind used routinely for formalin-fixed samples — for dehydration and paraffin infiltration of PAXgene Tissue treated samples?
FAQ ID -3032
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Are PAXgene Tissue Containers suitable for long term-storage at freezing temperatures?
FAQ ID -3030
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Is it possible to manually process PAXgene Tissue treated samples without using an automated processor?
FAQ ID -3036
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Is a special processing protocol needed?
FAQ ID -3035
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Is it possible to process formalin-fixed and PAXgene Tissue fixed samples together in one run?
FAQ ID -3033
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Is it necessary to clean a processor normally used for formalin-fixed tissue before using it with PAXgene Tissue fixed tissue?
FAQ ID -3034
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Can sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for other histochemical staining techniques, such as PAS?
FAQ ID -3041
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When should the N2 bottle used with the QIAxcel Advanced System be changed?
FAQ ID -3001
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Are the multicopy reference assays for Type-it CNV PCR +qC Kits and the qBiomarker assays the same?
FAQ ID -3000
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How should I reconstitute the lyophilized RNase-Free DNase Set with the RNeasy 96 kit?
FAQ ID -3003
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How do I clean up RNA preparations containing miRNA?
FAQ ID -3002
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What is the thermal output for a BioRobot MDx and BioRobot Universal?
FAQ ID -3056
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What is the thermal output for a QIAsymphony SP and AS instrument?
FAQ ID -3057
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Can I use Tungsten Carbide beads for RNA extraction protocols using QIAGEN Kits?
FAQ ID -3053
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What is the minimum number of samples that can be extracted on a BioSprint 96?
FAQ ID -3076
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What is the fixation method used in the PAXgene Tissue System?
FAQ ID -3019
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What does PAXgene Tissue FIX contain?
FAQ ID -3020
View
Can I perform the IDH 1/2 RGQ PCR test with slides containing less than 40% tumor cells?
FAQ ID -3098
View
What volume of bacterial culture can be processed using the QuickLyse Miniprep Kit?
FAQ ID -1357
View
Can the MaXtract High Density Tube be used with QIAzol to isolate RNA?
FAQ ID -1304
View
I have a Human Druggable Genome siRNA Set V2.0. Can I reorder the siRNAs from this set?
FAQ ID -1366
View
What factors determine if denatured proteins accumulate underneath, or inside the gel of the MaXtract Low and High Density Tubes?
FAQ ID -1312
View
If both MaXtract Low Density and High Density is suitable for an application, which type is preferable to use?
FAQ ID -1310
View
How will service work carried out on my QIAGEN BioRobot be documented?
FAQ ID -1331
View
If I cannot order FlexiPlate siRNA online, how do I order?
FAQ ID -1371
View
What information about my gene or siRNA do I need to order FlexiPlate siRNA?
FAQ ID -1365
View
Is it possible to use MaXtract for an organic extraction with a mixture of phenol and BCP (1 bromine chlorpropane)?
-3
View
What information is provided with FlexiPlate siRNAs?
FAQ ID -1363
View
Is it possible to elute plasmid DNA obtained with the QuickLyse Miniprep Kit in nuclease-free water?
FAQ ID -1355
View
Which formats are available for FlexiPlate siRNA?
FAQ ID -1358
View
Which parts are exchanged during a Preventive Maintenance visit for my QIAGEN BioRobot?
FAQ ID -1334
View
When isolating DNA from plant cells using CTAB, should MaXtract Low or High Density be used?
FAQ ID -1308
View
Which controls can I choose for FlexiPlate siRNA?
FAQ ID -1368
View
Will the REPLI-g UltraFast Mini Kit reaction always yield 10 µg DNA?
FAQ ID -1328
View
Why is there DNA in the no-template control reaction when using the standard REPLI-g procedure, but not when using the UltraFast procedure?
FAQ ID -1327
View
Which density gel type is contained in the yellow or green MaXtract Tubes?
FAQ ID -1315
View
What is the composition of FlexiPlate siRNA buffer?
FAQ ID -1361
View
Which software updates are included in a Premium Cover Agreement for my QIAGEN Instrument?
FAQ ID -1336
View
What is the composition of elution buffer QLE in the QuickLyse Miniprep Kit?
FAQ ID -1356
View
Can I reduce the amount of starting material below 10 ng when using the REPLI-g UltraFast Mini Kit?
FAQ ID -1325
View
Can MaXtract tubes be autoclaved prior to use?
FAQ ID -1305
View
Can I reduce the reaction time to e.g., 30 minutes, when using the REPLI-g UltraFast Mini Kit?
FAQ ID -1326
View
Can I use any type of DNA extraction method to purify template DNA for amplification using the REPLI-g Mitochondrial DNA Kit?
FAQ ID -1321
View
Can the bacterial pellet in the QuickLyse Miniprep procedure be resuspended by pipetting up and down or shortening the vortexing time?
FAQ ID -1352
View
Can several extraction steps be performed in the same MaXtract tube?
FAQ ID -1302
View
Can a phenol-chloroform ratio different from 1:1 also be used with MaXtract Low and High Density?
FAQ ID -1309
View
Can DNA isolated with MaXtract tubes be used for downstream applications such as restriction digestion, labeling, blotting, PCR, and automated sequencing?
FAQ ID -1303
View
Can I amplify mitochondrial DNA directly from whole blood or cells using the REPLI-g Mitochondrial DNA Kit?
FAQ ID -1319
View
Can I order Alexa Fluor labeled controls with FlexiPlate siRNA?
FAQ ID -1369
View
Can frozen sample material pulverized in liquid nitrogen be directly added to MaXtract tubes?
FAQ ID -1301
View
Can HP Validated siRNAs be ordered on the FlexiPlate?
FAQ ID -1367
View
Can I also screen large constructs like BACs, fosmids, and cosmids using the QIAGEN PlasmidAmp Kit?
FAQ ID -1337
View
How do I resuspend my FlexiPlate siRNAs?
FAQ ID -1360
View
How much REPLI-g amplified mitochondrial DNA should be used in a subsequent PCR reaction?
FAQ ID -1320
View
Do mutations in the target mitochondrial DNA prevent primer binding, resulting in no DNA amplification with the REPLI-g Mitochondrial DNA Kit?
FAQ ID -1323
View
Do you have stability data for genomic DNA isolated with the BioSprint Kits?
FAQ ID -1318
View
Does the addition of sample and organic solution to the MaXtract Tubes require a specific pipetting sequence?
FAQ ID -1307
View
Do you have stability data for genomic DNA isolated with the Flexigene DNA Kit?
FAQ ID -1317
View
How does a Service Support Agreement provide defined budgeting for maintaining my BioRobot?
FAQ ID -1330
View
How can I upload my gene list for FlexiPlate siRNA to GeneGlobe?
FAQ ID -1364
View
How can I increase the DNA yield from low-copy plasmids using the QuickLyse Miniprep Kit?
FAQ ID -1354
View
How can I ensure optimal performance of my QIAGEN instrument?
FAQ ID -1329
View
What is the "seed sequence" of a miRNA?
FAQ ID -2262
View
What is the turn around time for the ordering of miScript Target Protector?
FAQ ID -2254
View
What is the sample throughput of Pyrosequencing systems?
FAQ ID -2215
View
What is the processing time for 96 normalizations with the SeqTarget System?
FAQ ID -2237
View
What is the optimal range of PCR fragment sizes for normalization with the SeqTarget System?
FAQ ID -2247
View
What method do you recommend to quantify DNA before running the KRAS and EGFR PCR kit?
FAQ ID -2292
View
What technologies are used by the KRAS and EGFR PCR kits?
FAQ ID -2286
View
What kind of automation can be done with the EasyXtal 15-well Tool?
FAQ ID -2220
View
What kind of reading length can I expect when using Pyrosequencing technology for sequence analysis?
FAQ ID -2216
View
What dyes are used in the KRAS and EGFR PCR kits?
FAQ ID -2288
View
What do I do if I get amplification in my no template control (NTC) with the KRAS and EGFR PCR kits?
FAQ ID -2298
View
What format are the EasyXtal 15-well tools?
FAQ ID -2219
View
What do I need to provide when ordering a miScript Target Protector?
FAQ ID -2251
View
What is the difference of the sequence of a miScript miRNA Inhibitor and miScript Target Protector?
FAQ ID -2253
View
What is a miScript Precursor Assay?
FAQ ID -2214
View
What is the difference between probability and typicality in the Rotor-Gene ScreenClust HRM Software?
FAQ ID -2203
View
What is a miScript Target Protector?
FAQ ID -2249
View
Which transfection reagent is recommended for transfection of the miScript Target Protectors?
FAQ ID -2252
View
Which variants of the EasyXtal 15-well tool are available?
FAQ ID -2221
View
Why are most of my samples outside of the cluster in supervised mode using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2205
View
Why are some of my samples outside of the cluster using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2204
View
Why does the SeqTarget Prep Protocol require a 2-step procedure?
FAQ ID -2238
View
Why does the miScript Target Protector not induce the degradation process?
FAQ ID -2263
View
Why do I have wavy DNA bands on my agarose gel?
FAQ ID -2260
View
What will happen, if the normalized DNA for the SeqTarget System is eluted with Buffer EB instead of EB2?
FAQ ID -2242
View
When negative results are generated using the KRAS and EGFR PCR kits, do I rerun them?
FAQ ID -2294
View
What type of samples can I use for the KRAS PCR and EGFR kits?
FAQ ID -2293
View
What voltage should be used to run my agarose gel with the GelPilot Agarose?
FAQ ID -2261
View
Which mode should I use in the Rotor-Gene ScreenClust HRM Software, supervised or unsupervised?
FAQ ID -2202
View
Does the atypical look of the lyophilized FlexiTube siRNA Premix have a negative impact on performance?
FAQ ID -2267
View
Does the miScript Target Protector match the target sequence?
FAQ ID -2250
View
Do the KRAS and EGFR PCR kits contain a passive reference dye?
FAQ ID -2295
View
Do I have to order at least 4 FlexiTube siRNA Premixes?
FAQ ID -2266
View
How do I store my FlexiTube siRNA Premix?
FAQ ID -2270
View
How is ARMS technology superior to alternative methods such as dye terminator sequencing?
FAQ ID -2290
View
Effects of low A260/A230 ratios in RNA preparations on downstream applications
FAQ ID -2248
View
How can I check the success of the normalization procedure for the SeqTarget System?
FAQ ID -2245
View
How do I search for all the miScript Target Protectors for one species?
FAQ ID -2255
View
Can PCR fragments be normalized for the SeqTarget System after purification with QIAquick?
FAQ ID -2240
View
Can PCR fragments be normalized for the SeqTarget System without a previous purification step?
FAQ ID -2246
View
Can I use a 384 well plate for the KRAS and EGFR PCR kits?
FAQ ID -2296
View
Are all 3 components of the SeqTarget System required or can a different Long Range PCR Kit and primers be used?
FAQ ID -2234
View
Can the miScript Precursor Assay differentiate between pri- and pre-miRNA?
FAQ ID -2211
View
Does ethanol from the PCR product purification influence the normalization procedure for the SeqTarget System?
FAQ ID -2244
View
What are EasyXtal 15-well Tools used for?
FAQ ID -2217
View
What are Principal Components analyzed in Rotor-Gene ScreenClust HRM Software?
FAQ ID -2200
View
Is the SeqTarget System compatible with standard-size PCRs?
FAQ ID -2241
View
What are the amplicon lengths of the KRAS and EGFR PCR kits?
FAQ ID -2289
View
What are clusters analyzed in the Rotor-Gene ScreenClust HRM Software?
FAQ ID -2201
View
What does QIAGEN use for a positive control in precursor detection experiments?
FAQ ID -2212
View
What controls are used in the KRAS and EGFR PCR kits to ensure reliable results?
FAQ ID -2291
View
What do I do if I cannot see amplification in the FAM channel in my KRAS and EGFR PCR kits?
FAQ ID -2297
View
What are the corresponding QIAGEN names for former Biotage instruments?
FAQ ID -2285
View
What buffer conditions give the best resolution for agarose gel electrophoresis?
FAQ ID -2256
View
What can be the reason for low DNA yields after normalization with the SeqTarget System?
FAQ ID -2239
View
How much buffer should be used for agarose gel electrophoresis?
FAQ ID -2257
View
How much DNA should be loaded per well of an agarose gel?
FAQ ID -2259
View
How should gels be cast with the GelPilot Agarose so that optimal resolution is achieved?
FAQ ID -2258
View
How long does it take to generate results using the KRAS and EGFR PCR kits?
FAQ ID -2299
View
How long should PCR fragments be for the SeqTarget System?
FAQ ID -2236
View
How many miScript Precursor Assays do you offer?
FAQ ID -2213
View
Is it possible to use lower siRNA concentrations with FlexiTube siRNA Premix?
FAQ ID -2264
View
Is one able to use FlexiTube siRNA Premix for transfection of primary adherent cell types?
FAQ ID -2268
View
Is one able to use FlexiTube siRNA Premix for transfection of primary suspension cell types?
FAQ ID -2269
View
If I am working with a difficult-to-transfect cell type or if I obtain only weak silencing effects what can I do?
FAQ ID -2265
View
Is it possible to normalize to more or less than 500 ng with the SeqTarget System if needed?
FAQ ID -2235
View
Is it possible to purify and normalize long range PCRs produced with PCR kits from other vendors for the SeqTarget System?
FAQ ID -2243
View
Is it possible to purify viral RNA using FastLane Cell RT-PCR Kits?
FAQ ID -1484
View
Why is the RT step with the QuantiFast RT Kits much shorter compared to QuantiTect RT Kits?
FAQ ID -1451
View
What applications are offered on the QIAcube?
FAQ ID -1403
View
Why is the activation time for HotStarTaq Plus Polymerase in the QuantiFast SYBR Green Kits different from that for QuantiFast Probe Kits?
FAQ ID -1449
View
Why is the storage time for QuantiFast PCR Kits shorter than that for QuantiTect PCR Kits?
FAQ ID -1446
View
Why is the reaction volume for QuantiFast PCR Kits lower than that for QuantiTect PCR Kits?
FAQ ID -1447
View
Why is the QuantiFast denaturation step different for PCR and RT-PCR runs in the two-step protocol for the ABI 7500 and other cyclers?
FAQ ID -1442
View
What is the minimum number of siRNAs per order? If I order 40 siRNAs, can I get those on 2 plates?
FAQ ID -1468
View
Can purification columns from other suppliers be processed on the QIAcube?
FAQ ID -1406
View
Can QIAcube protocol runs be interrupted, and subsequently be continued to completion?
FAQ ID -1414
View
Are run reports and/or log files available on the QIAcube?
FAQ ID -1412
View
Can I use more than 4 µl FastLane lysate in subsequent real-time RT-PCR?
FAQ ID -1480
View
Are FlexiPlate siRNAs available at a higher final concentration than 10 µM?
FAQ ID -1476
View
Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with QuantiFast Probe PCR Kits?
FAQ ID -1452
View
Can different amounts of siRNA be ordered on one FlexiPlate?
FAQ ID -1473
View
Can 2 µl reaction volumes be used with QuantiFast PCR Kits?
FAQ ID -1440
View
Are there any specific cleaning solutions recommended for the QIAcube?
FAQ ID -1416
View
Do the QuantiTect Primer Assays work with QuantiFast SYBR Green Kits?
FAQ ID -1439
View
Do special settings have to be used for QuantiFast PCR Kits on the Eppendorf Mastercycler ep realplex?
FAQ ID -1437
View
Do I need to buy special kits for the QIAcube?
FAQ ID -1402
View
Do I need to discard partially used QIAcube tip racks?
FAQ ID -1418
View
What information is required to reorder specific siRNAs from a siRNA Set in FlexiPlate format?
FAQ ID -1463
View
What are the turnaround times for FlexiPlate siRNA orders?
FAQ ID -1478
View
What are the dimensions and weight of the QIAcube?
FAQ ID -1407
View
How much time will be saved when switching from standard cycling to fast cycling with QuantiFast Kits?
FAQ ID -1438
View
How many reactions can I perform with the new QuantiFast Kits for real-time PCR?
FAQ ID -1425
View
If FlexiPlate siRNA was accidentally redissolved in siRNA Suspension Buffer instead of water, resulting in a 2x buffer, would it affect performance of the siRNAs?
FAQ ID -1477
View
How do you achieve fast cycling, yet still deliver the same performance in PCR as that achieved with standard cycling?
FAQ ID -1430
View
Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube?
FAQ ID -1415
View
Why is a 2-step (and not a 3-step) cycling protocol recommended for QuantiFast SYBR Green PCR Kits?
FAQ ID -1450
View
Why do QuantiFast SYBR Green PCR Kits require such a high primer concentration?
FAQ ID -1443
View
What QuantiFast Kit should be used on the Eppendorf Mastercycler ep realplex?
FAQ ID -1436
View
What is the detection limit of the QuantiFast Kits for real-time PCR?
FAQ ID -1432
View
Which kind of 96 and 384-well plates are available for FlexiPlate siRNAs?
FAQ ID -1459
View
Which cell lines have been tested with FastLane Cell RT-PCR Kits?
FAQ ID -1485
View
Can I adjust the ROX concentration in the QuantiFast master mix?
FAQ ID -1427
View
Can FlexiPlate siRNAs be ordered in plates other than the standard NUNC 96 well V bottom plates?
FAQ ID -1458
View
Can I follow the reaction setup for probe-based assays described in Appendix D of the FastLane Cell RT-PCR Handbook using 384-well plates?
FAQ ID -1482
View
Can fast cycling be carried out with QuantiFast Probe PCR Kits and FRET probes?
FAQ ID -1433
View
Can FlexiPlate siRNA be ordered in solution on dry-ice?
FAQ ID -1474
View
Can I program my own protocols for the QIAcube?
FAQ ID -1405
View
How can I load new protocols onto the QIAcube?
FAQ ID -1421
View
How can I get software updates for the QIAcube?
FAQ ID -1413
View
How do QuantiFast PCR Kits compare to QuantiTect PCR Kits for quantitative real-time PCR?
FAQ ID -1441
View
Do you offer trial-kit sizes for the new QuantiFast Kits?
FAQ ID -1429
View
How can I decontaminate the QIAcube system?
FAQ ID -1419
View
Can the QIAcube heater/shaker be used independently from protocol runs?
FAQ ID -1422
View
Can the QIAcube rotor adapters be autoclaved?
FAQ ID -1409
View
Can the QIAcube centrifuge be used independently from protocol runs?
FAQ ID -1423
View
Can QuantiFast PCR Kits be used on real-time PCR instruments without fast cycling options?
FAQ ID -1428
View
Can the QIAcube be connected to a laboratory information management system?
FAQ ID -1411
View
Can the QIAcube rotor and/or buckets be removed for cleaning?
FAQ ID -1410
View
Does the high primer concentration required by QuantiFast SYBR Green PCR Kits impair annealing specificity?
FAQ ID -1444
View
Does the master mix in the QuantiFast Kits contain dUTP to allow UNG treatments?
FAQ ID -1431
View
Can the QuantiFast Probe PCR +ROX Vial mastermix be stored long-term after adding ROX dye?
FAQ ID -1435
View
What are the Tms of PCR products obtained using the miScript System?
FAQ ID -1599
View
Do you have a protocol for cleanup of REPLI-g amplified DNA?
FAQ ID -1545
View
What can be done if the protein pellet does not solubilize completely using the AllPrep DNA/RNA/Protein Mini Kit?
FAQ ID -1580
View
What controls should be used for normalizing miRNA real-time PCR data obtained with the miScript System?
FAQ ID -1596
View
What is Q-Bond used in the QIAGEN Fast Cycling PCR and QuantiFast Kits?
FAQ ID -1554
View
Will QuantiTect Primer Assays work at an annealing temperature of 60ºC with QuantiFast SYBR Green PCR and RT-PCR Kits?
FAQ ID -1553
View
Which homogenization method do you recommend for tissues stored in Allprotect Tissue Reagent?
FAQ ID -1572
View
Can different incubation times be used for the 2 hour amplification step in the QuantiTect Whole Transcriptome protocol?
-8
View
Does residual Allprotect Tissue Reagent left behind on tissue samples interfere with DNA, RNA, and protein purification?
FAQ ID -1573
View
Is cDNA generated with the QuantiTect Whole Transcriptome Kit suitable for use in microarray analysis?
FAQ ID -1587
View
Do you have a protocol for purification of genomic DNA from cultured cells using the QIAamp DNA Micro Kit?
FAQ ID -1547
View
Do you have a protocol for concentration of RNA in 96-well plates?
FAQ ID -1546
View
Do you have a protocol for purification of total RNA from fatty tissues using QIAzol Lysis Reagent and MaXtract High Density?
FAQ ID -1550
View
Which real-time PCR kits are recommended downstream of the QuantiTect Whole Transcriptome Kit?
FAQ ID -1592
View
What primers are used in the reverse-transcription step of the QuantiTect Whole Transcriptome procedure?
FAQ ID -1584
View
What is the maximum amount of RNA that can be used for amplification with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1591
View
Can the AllPrep DNA/RNA/Protein Mini Kit be used with fibrous or lipid tissues?
FAQ ID -1579
View
What starting amount of RNA purified using an miRNeasy Kit should be used with the miScript System?
FAQ ID -1597
View
Can samples in Allprotect Tissue Reagent undergo repeated freeze-thaws without degradation of DNA, RNA, and protein?
FAQ ID -1575
View
Can the QuantiFast Probe PCR +ROX Vial Kit be used on the ABI PRISM 7900 and Applied Biosystems 7500 cyclers?
FAQ ID -1555
View
Can RNA purified from formalin-fixed, paraffin-embedded (FFPE) tissue be used with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1583
View
Does Buffer APP in the AllPrep DNA/RNA/Protein Mini Kit contain acetone?
FAQ ID -1576
View
Can tissue homogenized in Buffer RLT Plus instead of Buffer RLT be used with the AllPrep DNA/RNA/Protein Mini Kit?
FAQ ID -1577
View
Does cDNA amplified with the QuantiTect Whole Transcriptome Kit correspond to full-length RNA transcripts?
FAQ ID -1585
View
Can tissue incubated in Allprotect Tissue Reagent be used for morphological studies?
FAQ ID -1571
View
Can less than 10 ng RNA be used with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1590
View
Can Allprotect Tissue Reagent be used to stabilize RNA, DNA and proteins in cells, bacteria, yeast, and plants?
FAQ ID -1574
View
Can degraded RNA be used with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1586
View
How much cDNA is sufficient for real-time PCR detection of miRNA transcripts generated with the miScript System?
FAQ ID -1598
View
How many samples can be run in parallel with the Rotor-Gene Q real-time PCR instrument?
FAQ ID -1519
View
What is the advantage of EpiTect MethyLight PCR Kits over QuantiTect Probe PCR Kits for methylation studies?
FAQ ID -2010
View
What is the advantage of using the Type-it Microsatellite PCR Kit?
FAQ ID -2059
View
What is the advantage of using the Type-it Mutation Detect PCR Kit?
FAQ ID -2064
View
What is a good starting amount of template DNA for PCR with the EpiTect MSP Kit?
FAQ ID -2005
View
What is elution Buffer ATE of the QIAsymphony DNA Investigator Kit?
FAQ ID -2029
View
What is the maximum lifetime of the QIAGEN rotor for the Centrifuge 4 and 4K?
-16
View
What is the maximum DNA yield from forensic samples processed with the QIAsymphony SP?
FAQ ID -2028
View
What is the principle behind the DNA Polymerase in the EpiTect MSP Kit?
FAQ ID -2003
View
Is it possible to use different sample types in one run with the MagAttract 96 cador Pathogen Kit?
FAQ ID -2072
View
What is the purpose of the unmethylated and unconverted control DNA of the EpiTect PCR Control DNA Set?
FAQ ID -2007
View
Is magnetic particle carry-over in the eluates on the BioSprint 96 normal?
FAQ ID -2073
View
What happens when using EpiTect MethyLight Assays on only partially bisulfite converted DNA template?
FAQ ID -2009
View
Should sample lysate volumes be exactly 200 µl, 500 µl, or 1000 µl when loaded on the QIAsymphony SP?
FAQ ID -2031
View
What are the expected PCR results when using EpiTect Control DNA on untreated or bisulfite-converted DNA?
FAQ ID -2011
View
What are the features of the expression vector used for QIAgenes Insect/Mammalia?
FAQ ID -2050
View
Why do casework-sample protocols for the QIAsymphony DNA Investigator Kit start with different lysate volumes (200 µl, 500 µl, or 1000 µl)?
FAQ ID -2035
View
Why is CoralLoad included in the Type-it Mutation Detect, but not in the Type-it Microsatellite PCR Kit?
FAQ ID -2068
View
Why is there more than one QIAgene for my gene of interest?
FAQ ID -2044
View
Why do pQE-T7 vectors of the QIAgenes E. coli system have an additional UAG amber stop codon?
FAQ ID -2043
View
Why is the Type-it Mutation Detect PCR Kit recommended for preamplification of SNPs?
FAQ ID -2067
View
Will the optimized DNA sequence for QIAgenes be provided?
FAQ ID -2045
View
Why is maximum amplicon size for the Type-it Microsatellite PCR Kit limited to 500 bp?
FAQ ID -2060
View
Why is special Type-it Fast SNP Probe PCR chemistry required for TaqMan® SNP Genotyping?
FAQ ID -2057
View
What sample volumes can be processed with the QIAsymphony Virus/Bacteria Kits?
FAQ ID -2037
View
What sample types can be processed using the QIAsymphony Virus/Bacteria protocols?
FAQ ID -2036
View
Can data be analyzed on the Rotor-Gene Q while a run is in progress?
FAQ ID -2091
View
Are there two vectors for protein expression using QIAgenes in insect or mammalian cells?
FAQ ID -2048
View
Are there recommendations for sensitive detection of underrepresented fragments using the Type-it Mutation Detect PCR Kit?
FAQ ID -2066
View
Can other probe technologies besides TaqMan® be used with the Type-it Fast SNP Probe PCR Kit?
FAQ ID -2058
View
Is mitochondrial DNA isolated with the QIAsymphony DNA Investigator Kit on the QIAsymphony SP?
FAQ ID -2030
View
Is a passive internal reference dye, like ROX, required on the Rotor-Gene Q to obtain reproducible results?
FAQ ID -2079
View
Is it possible to edit the sample sheet in the Rotor-Gene Q software after a PCR run?
FAQ ID -2092
View
Is it possible to import a standard curve from a previous PCR run on the Rotor-Gene Q?
FAQ ID -2093
View
What are the ideal and the largest PCR amplicon sizes when using the EpiTect MSP Kit?
FAQ ID -2004
View
What kind of samples should be processed using the large-volume protocol with the QIAsymphony DNA Investigator Kit?
FAQ ID -2034
View
Which expression systems can be used with the QIAgenes Insect/Mammalia expression constructs?
FAQ ID -2049
View
Which QIAsymphony Virus/Bacteria Kit size should be used for each protocol?
FAQ ID -2038
View
What types of reaction vessels are required for use in the Rotor-Gene Q?
FAQ ID -2085
View
Which capillary sequencers can be used for detection of amplicons from the Type-it Microsatellite PCR Kits?
FAQ ID -2063
View
Can the EpiTect PCR Control DNAs be used for mouse and rat assays?
FAQ ID -2008
View
Can the EpiTect PCR Control DNAs also be used as standard in real-time methylation specific quantification?
FAQ ID -2006
View
Can the protocols for purification of small RNAs from cells in the appendices of the AllPrep DNA/RNA 96 Handbook also be used with tissue samples?
FAQ ID -2001
View
Can solid samples be processed with the QIAsymphony DNA Investigator Kit on the instrument?
FAQ ID -2027
View
Can QIAGEN rotors used with the centrifuges 4C and 4KC also be used with 4-16 and 4-16K models?
-15
View
Can the MagAttract 96 cador Pathogen Kit also be used on the KingFisher 96 or MagMAX Express 96 instruments?
FAQ ID -2074
View
Do all QIAsymphony DNA Investigator protocols use carrier RNA?
FAQ ID -2033
View
Can TopTaq Master Mix simply be stored at room temperature on the lab bench for up to 4 months?
FAQ ID -2052
View
Can the Type-it Mutation Detect PCR kit be used for multiplex PCR of fragments longer than 500 bp?
FAQ ID -2065
View
Can the Type-it Microsaltellite PCR Kit be used on cyclers with fast ramping rates?
FAQ ID -2062
View
Can 0.1 ml and 0.2 ml tubes used on the Rotor-Gene Q be labeled?
FAQ ID -2082
View
How many signal readings are taken during the real-time data acquisition on the Rotor-Gene Q?
FAQ ID -2083
View
Can membrane proteins be expressed with QIAgenes expression kits?
FAQ ID -2047
View
Can DNase digestion be performed on the RNeasy 96 plate during the RNA purification part of the Allprep DNA/RNA 96 procedure?
FAQ ID -2000
View
Does an internal control have to be included for each QIAsymphony Virus/Bacteria protocol run?
FAQ ID -2040
View
Do QIAsymphony Virus/Bacteria samples require pretreatment before they are loaded onto the instrument?
FAQ ID -2039
View
Does the centrifugal force have any effect on the kinetics of a PCR reaction on the Rotor-Gene Q?
FAQ ID -2081
View
How can a C-terminal 6xHis-tagged QIAgene be expressed?
FAQ ID -2041
View
How can full-length genes or individual domains of the QIAgenes Insect/Mammalia constructs be subcloned?
FAQ ID -2051
View
Does the Rotor-Gene Q software have the capacity to export data?
FAQ ID -2089
View
Can DNA yields be increased by prolonging incubation with proteinase K in Buffer ATL?
FAQ ID -2032
View
Can frozen samples be used with the MagAttract 96 cador Pathogen Kit?
FAQ ID -2070
View
How fast does the rotor spin during a run on the Rotor-Gene Q?
FAQ ID -2080
View
How is the log graph threshold value (Ct) for the Rotor-Gene Q determined?
FAQ ID -2088
View
How long can DNA converted with EpiTect Bisulfite Kits be stored before use in methylation specific PCR with the EpiTect MSP Kit?
FAQ ID -2002
View
How does the built-in QC control for complete bisulfite conversion of DNA work on PyroMark Q24?
FAQ ID -2095
View
How will running less than 96 samples with the MagAttract 96 cador Pathogen kit affect usage of plasticware?
FAQ ID -2071
View
If orders cannot be placed online, how can QIAgenes be ordered?
FAQ ID -2046
View
If new dyes or chemistries appear on the real-time PCR market, will Rotor-Gene Q be capable of running those dyes?
FAQ ID -2087
View
How long does a single run on the PyroMark Q24 take?
FAQ ID -2096
View
How much space does the PyroMark Q24 instrument need?
FAQ ID -2098
View
Does the Type-it Microsatellite PCR Kit always require an annealing temperature of 60°C, or can pre-optimized annealing temperatures for target-primer systems be used?
FAQ ID -2061
View
How accurate and reliable is PyroMark Q24 in mutation analysis?
FAQ ID -2094
View
Is there a charge for user training on the QIAsymphony SP System?
FAQ ID -1900
View
Is the QIAsymphony RNA Kit compatible with stabilized samples?
FAQ ID -1944
View
Is sample pretreatment required for purifying RNA on the QIAsymphony SP?
FAQ ID -1938
View
Is RNA co-purified with DNA using QIAsymphony DNA Tissue protocols?
FAQ ID -1920
View
What are the expected DNA yields from tissue processed on the QIAsymphony SP?
FAQ ID -1919
View
QuantiTect Primer Assays are bioinformatically validated, genomewide primer sets. What does “bioinformatically validated” mean?
FAQ ID -1982
View
What are the dimensions and weight of the QIAsymphony SP System?
FAQ ID -1901
View
What is the range of elution volumes on the QIAsymphony SP?
FAQ ID -1908
View
How many PCR reactions can be performed for different scales of miScript Primer Assays?
FAQ ID -1990
View
How many samples can be processed per kit or per reagent cartridge for RNA purification on the QIAsymphony SP?
FAQ ID -1940
View
How does QIAsymphony SP keep cellular samples in suspension prior to pipetting so that samples do not settle?
FAQ ID -1905
View
How efficient is DNA removal by a gDNA Eliminator 96 plate compared with DNase digestion on an RNeasy 96 plate?
FAQ ID -1994
View
How long can homogenates remain on the QIAsymphony SP without risk of RNA degradation?
FAQ ID -1941
View
How much sample can be used with the Allprep DNA/RNA 96 Kit?
FAQ ID -1997
View
How much sample can be used with the RNeasy Plus 96 Kit?
FAQ ID -1992
View
How much starting material can be used with the QIAsymphony DNA Mini Kit on the QIAsymphony SP?
FAQ ID -1917
View
What sample types can be used with the AllPrep DNA/RNA 96 Kit?
FAQ ID -1996
View
Which types and amounts of sample material can be processed for RNA extraction on the QIAsymphony SP?
FAQ ID -1937
View
What sample types can be used with the RNeasy Plus 96 Kit?
FAQ ID -1991
View
Will mitochondrial DNA also be isolated using the DNA Tissue protocols on the QIAsymphony SP?
FAQ ID -1921
View
What sample types should be processed with the High Content (HC) protocol for DNA extraction on the QIAsymphony SP?
FAQ ID -1918
View
Why does the Allprep DNA/RNA 96 Kit use Buffer RLT, whereas the AllPrep DNA/RNA Mini Kit uses Buffer RLT Plus?
FAQ ID -1999
View
Why is there no vacuum-only protocol for the RNeasy Plus 96 Kit?
FAQ ID -1993
View
What is the turn around time for custom miScript miRNA mimics, inhibitors and primer assays?
FAQ ID -1985
View
Is it possible to modify QIAsymphony protocols?
FAQ ID -1903
View
What are the starting volumes of homogenate for RNA extraction on the QIAsymphony SP?
FAQ ID -1939
View
What are the expected yields of total RNA isolated with the QIAsymphony SP?
FAQ ID -1942
View
What kind of molecules are miScript miRNA Inhibitors?
FAQ ID -1983
View
What sample input volumes can be used on the QIAsymphony SP?
FAQ ID -1907
View
What sample types can be processed using the tissue protocols for DNA extraction on the QIAsymphony SP?
FAQ ID -1915
View
What kind of molecules are miScript miRNA Mimics?
FAQ ID -1986
View
What RNA elution volumes are available with the QIAsymphony RNA Kit?
FAQ ID -1943
View
Do samples require pretreatment before loading onto the QIAsymphony SP for DNA extraction?
FAQ ID -1916
View
Do limiting primer concentrations need to be determined when using QuantiFast Multiplex PCR Kits?
FAQ ID -1976
View
Can uneven sample numbers be processed on the QIAsymphony SP module?
FAQ ID -1906
View
Does the QIAsymphony SP system generate run reports and/or log files?
FAQ ID -1911
View
Does HiPerFect work for the transfection of miScript miRNA mimics and inhibitors?
FAQ ID -1984
View
Do you have a protocol for the purification of archive-quality DNA from 0.5–20 mg paraffin-embedded tissue using Gentra Puregene Kits?
FAQ ID -1964
View
Do you have a protocol for the purification of archive-quality DNA from 1 ml of whole blood with the Gentra Puregene Tissue Kit?
FAQ ID -1953
View
Do you have a protocol for the purification of archive-quality DNA from 100 to 5 x 10e8 cultured cells using Gentra Puregene Kits?
FAQ ID -1960
View
Do you have a protocol for rapid purification of archive-quality DNA from up to 9 x 10e6 cells using Gentra Puregene Kits?
FAQ ID -1969
View
Can I program my own protocols for the QIAsymphony SP?
FAQ ID -1904
View
Are QuantiFast Multiplex PCR Kits available in trial sizes?
FAQ ID -1981
View
Are there any specific cleaning solutions recommended for the QIAsymphony SP?
FAQ ID -1913
View
Can 4-plex, real-time PCR be performed on the ABI PRISM 7000, 7700, or 7900 using the QuantiFast Multiplex PCR Kit?
FAQ ID -1978
View
Can the QIAsymphony RNA Kit be used to purify RNA from blood?
FAQ ID -1945
View
Can the QIAsymphony SP Module be connected to a laboratory information management system (LIMS)?
FAQ ID -1909
View
Can protocol runs on the QIAsymphony SP be interrupted and resumed?
FAQ ID -1912
View
Can the QuantiFast Multiplex PCR Kits be used on Roche LightCycler systems with TaqMan® probes?
FAQ ID -1979
View
Can the protocols for purification of small RNAs from cells in the appendices of the RNeasy Plus 96 Handbook also be used with tissue samples?
FAQ ID -1995
View
Do you have a protocol for the purification of archive-quality DNA from marine invertebrate tissue using Gentra Puregene Kits?
FAQ ID -1950
View
Do you have a protocol for the purification of archive-quality DNA from nematodes using Gentra Puregene Kits?
FAQ ID -1951
View
Do you have a protocol for the purification of archive-quality DNA from human dried blood using Gentra Puregene Kits?
FAQ ID -1959
View
Do you have a protocol for the purification of archive-quality DNA from Gram-negative bacteria using Gentra Puregene Kits?
FAQ ID -1975
View
Do you have a protocol for the purification of archive-quality DNA from Gram-positive bacteria using Gentra Puregene Kits?
FAQ ID -1946
View
Do you have a protocol for the purification of archive-quality DNA from hair roots using Gentra Puregene Kits?
FAQ ID -1947
View
Do you have a protocol for the purification of DNA from fruit flies?
FAQ ID -1970
View
Do you have a protocol for the purification of archive-quality DNA from tissue fixed in ethanol or formalin using Gentra Puregene Kits?
FAQ ID -1974
View
How can the QIAsymphony SP system be decontaminated?
FAQ ID -1914
View
Do you have a protocol for the purification of archive-quality DNA from umbilical cord blood using Gentra Puregene Kits?
FAQ ID -1958
View
Do you have a protocol for the purification of archive-quality DNA from buffy coat using Gentra Puregene Kits?
FAQ ID -1955
View
Do you have a protocol for the purification of archive-quality DNA from whole blood using Gentra Puregene Kits?
FAQ ID -1954
View
Do you have a protocol for the purification of archive-quality DNA from cerebrospinal fluid (CSF) using Gentra Puregene Kits?
FAQ ID -1967
View
Do you have a protocol for the purification of archive-quality DNA from Chlamydomonas using Gentra Puregene Kits?
FAQ ID -1968
View
Do you have a protocol for the purification of archive-quality DNA from blood smears using Gentra Puregene Kits?
FAQ ID -1948
View
Do you have a protocol for the purification of archive-quality DNA from fecal cells using Gentra Puregene Kits?
FAQ ID -1972
View
Do you have a protocol for the purification of archive-quality DNA from fish using Gentra Puregene Kits?
FAQ ID -1973
View
Do you have a protocol for the purification of archive-quality DNA from fungus using Gentra Puregene Kits?
FAQ ID -1971
View
Do you have a protocol for the purification of archive-quality DNA from buccal brushes using Gentra Puregene Kits?
FAQ ID -1965
View
Do you have a protocol for the purification of archive-quality DNA from Eimeria oocysts using Gentra Puregene Kits?
FAQ ID -1952
View
What effect does homogenization have on DNA yield and integrity when using AllPrep DNA/RNA Kits?
FAQ ID -1751
View
What is the fidelity of TopTaq DNA Polymerase?
FAQ ID -1739
View
Is TopTaq DNA Polymerase a hot-start polymerase?
FAQ ID -1748
View
Which nucleotide analogs can be used with TopTaq DNA Polymerase?
FAQ ID -1741
View
Why might Affymetrix GeneChip Mapping assays interfere with the REPLI-g FFPE kit?
FAQ ID -1755
View
What is the upper length of PCR products that can be amplified with TopTaq DNA Polymerase and Master Mix Kits?
FAQ ID -1738
View
What is the shelf-life of TopTaq DNA Polymerase and Master Mix?
FAQ ID -1743
View
What is the principle of the REPLI-g FFPE Kit?
FAQ ID -1753
View
What is the largest plasmid size that can be purified using the QuickLyse Miniprep Kit?
FAQ ID -1752
View
What is the minimum amount of starting material that can be used with the RNeasy Plus Micro and AllPrep DNA/RNA Micro Kit?
FAQ ID -1750
View
Can small miRNA-containing RNA fractions be separated from large RNAs using the miRNeasy FFPE Kit?
FAQ ID -1737
View
What is the recommended sample size for use with the REPLI-g FFPE Kit?
FAQ ID -1756
View
Can miRNA and other RNAs smaller than 200 nucleotides be isolated with the AllPrep DNA/RNA Micro Kit or RNeasy Plus Micro Kit?
FAQ ID -1749
View
Are there problems with the default threshold setting on the Applied Biosystems 7500 when using QuantiFast Probe PCR Kits?
FAQ ID -1702
View
Can TA/UA cloning be done with PCR products amplified with TopTaq DNA Polmyerase and TopTaq Master Mix Kits?
FAQ ID -1740
View
At which step in the REPLI-g FFPE protocol should PicoGreen quantification be done?
FAQ ID -1758
View
Have QuantiTect Primer Assays been tested with QuantiFast SYBR Green PCR Kits on the Mastercycler ep realplex?
FAQ ID -1714
View
How can foaming of Buffer RLT Plus lysates be avoided when using RNeasy Plus Micro and Mini Kits, or the Allprep DNA/RNA Mini Kit?
FAQ ID -1734
View
Do you have protocols for sample processing on the Autopure LS instrument?
FAQ ID -1759
View
Can the REPLI-g FFPE procedure restore DNA derived from FFPE tissue samples in the original order?
FAQ ID -1754
View
Can the REPLI-g FFPE Kit also be used for formalin-fixed, ethanol preserved samples?
FAQ ID -1760
View
Do you have a protocol for the isolation of total RNA from paraffin-embedded sections on the Biorobot M48?
FAQ ID -1733
View
Does TopTaq Stabilizer of the TopTaq DNA Polymerase and Master Mix Kits interfere with downstream applications?
FAQ ID -1744
View
Do you have a forensic post-PCR purification protocol to purify double-stranded DNA fragments from PCR reactions?
FAQ ID -1761
View
Do CoralLoad dyes supplied in various QIAGEN PCR Kits interfere with downstream applications?
FAQ ID -1745
View
Is FlexiTube GeneSolution available for other scales than the 1 nmol scale?
FAQ ID -1666
View
What buffer should be used to dilute cDNA made using the miScript Reverse Transcription Kit?
FAQ ID -1601
View
What is the binding capacity of the Ni-NTA Superflow Cartridges?
FAQ ID -1603
View
Is it necessary to clean up cDNA prepared with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1610
View
Is the master mix of QuantiFast Kits for real-time PCR aliquoted into several tubes to prevent cross-contamination?
FAQ ID -1697
View
Is the QuantiTect Whole Transcriptome Kit suitable for miRNA amplification?
FAQ ID -1609
View
Is sequence information for HP Validated siRNAs provided in the FlexiTube format?
FAQ ID -1665
View
Is it necessary to include a genomic DNA removal step with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1614
View
Which siRNA controls are available in FlexiTube format?
FAQ ID -1661
View
Where can I find background information and literature on Whole Genome Amplification with REPLI-g Kits?
FAQ ID -1690
View
Why is there DNA in the no-template (negative) control when using the QuantiTect Whole Transcriptome Kit?
FAQ ID -1620
View
What is the composition of Buffer PE?
FAQ ID -1691
View
What is the maximum volume of RNA in solution that can be used with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1616
View
Can the Ni-NTA Superflow Cartridges and/or Strep-Tactin Cartridges be connected in series?
FAQ ID -1606
View
Can the Ni-NTA and Strep-Tactin Superflow Cartridges be reused?
FAQ ID -1607
View
Can I store cDNA generated by the miScript System?
FAQ ID -1602
View
Can the QuantiTect Whole Transcriptome Kit be used for amplification of RNA from LCM samples?
FAQ ID -1612
View
Does the QuantiTect Whole Transcriptome Kit work with RNA purified from bacteria, yeast, or plants?
FAQ ID -1615
View
Can T-Script® enzyme of the QuantiTect Whole Transcriptome Kit be substituted by Quantiscript Reverse Transcriptase?
FAQ ID -1617
View
Can the QuantiTect Whole Transcriptome reaction be stopped after the reverse transcription step to proceed with the ligation and amplification steps at a later time?
FAQ ID -1618
View
Are FlexiTube siRNA and FlexiTube GeneSolution also available for rat siRNAs?
FAQ ID -1664
View
Can I still reorder siRNA that has been removed from the GeneGlobe data base?
FAQ ID -1668
View
Can amplification with the QuantiTect Whole Transcriptome Kit be extended to more than 8 hours to improve cDNA yields?
FAQ ID -1608
View
Can FlexiTube siRNA or FlexiTube GeneSolution products be ordered in solution?
FAQ ID -1667
View
Can I select HP Validated and HP GenomeWide siRNAs for FlexiTube?
FAQ ID -1658
View
Can cDNA prepared with the QuantiTect Whole Transcriptome Kit be reampliied using REPLI-g Kits?
FAQ ID -1611
View
How comparable is CoralLoad gel loading dye contained in various QIAGEN PCR Kits to Sigma Red?
FAQ ID -1644
View
How fast is the 6xHis-tagged protein purification process using Ni-NTA Superflow Cartridges?
FAQ ID -1605
View
How long is cDNA generated with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1613
View
How long can I archive cDNA generated with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1623
View
Have you observed co-amplification of genomic DNA from RNA templates used in the QuantiTect Whole Transcriptome Procedure?
FAQ ID -1619
View
Do I need to resuspend FlexiTube siRNAs in siRNA Resuspension buffer?
FAQ ID -1659
View
Do you have a protocol for purification of total RNA from plant latex?
FAQ ID -1694
View
Do I need to use the miScript Universal Primer for detection of mRNA?
FAQ ID -1600
View
Do you offer a QuantiFast Kit for one-step RT-PCR?
FAQ ID -1695
View
Do you have a protocol for purification of total RNA from plant tissue with the RNeasy Lipid Tissue Mini Kit?
FAQ ID -1693
View
How many samples can be processed with the QIAsymphony DNA Mini Kit and the Virus Blood 200 protocol?
FAQ ID -2159
View
How often can the Strep-Tactin Superflow Plus resin be reused?
FAQ ID -2169
View
If housekeeping and target genes differ significantly in abundance, will they both be amplified with equal efficiency using the Rotor-Gene Multiplex PCR Kit?
FAQ ID -2129
View
If Vapor-Lock is used to overlay PCR reactions, which volume should be entered as reaction volume in the Rotor-Gene Q Software?
FAQ ID -2150
View
Is it normal that the binding capacity of GST-resins decreases with flow rate?
FAQ ID -2174
View
Is it possible to purify a GST-tagged protein under denaturing conditions using Glutathione Superflow?
FAQ ID -2172
View
Why is EvaGreen instead of SYBR Green used as fluorescent dye in the Type-it HRM PCR Kit?
FAQ ID -2196
View
Why is the initial activation step different for Rotor-Gene Probe, SYBR Green and Multiplex Kits?
FAQ ID -2118
View
Why is a 2-step (and not a 3-step) cycling protocol recommended for Rotor-Gene SYBR Green Kits?
FAQ ID -2122
View
Why do I need normalization using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2198
View
Why is there an asterisk for my miScript Primer Assays?
FAQ ID -2176
View
Will Rotor-Gene Kits also work on the Rotor-Gene 6000 and 3000 cyclers?
FAQ ID -2121
View
Will Uracil-N-Glycosylase (UNG) completely remove contaminating amplicons when using QuantiTect +UNG Kits?
FAQ ID -2139
View
Will QuantiTect Primer Assays work with Rotor-Gene SYBR Green Kits using an annealing step at 60ºC?
FAQ ID -2124
View
What is miRNA*? Do I need to consider this in my profiling experiment?
FAQ ID -2179
View
What are the main differences between Rotor-Gene and QuantiTect or QuantiFast PCR Kits?
FAQ ID -2119
View
What are possible reasons for reduced DNA yields with REPLI-g Kits?
FAQ ID -2148
View
What is the residuals plot in the Rotor-Gene ScreenClust HRM Software?
FAQ ID -2199
View
What is the target nucleic acid for the Virus Blood 200 Protocol on the QIAsymphony?
FAQ ID -2158
View
What is the minimum number of assays that can be ordered on QuantiTect Primer Assay Plates?
FAQ ID -2109
View
What is the detection limit of the Rotor-Gene and QuantiFast Multiplex RT-PCR Kits?
FAQ ID -2144
View
What is the maximum number of targets that can be amplified per reaction with the QuantiFast Multiplex RT-PCR Kit?
FAQ ID -2145
View
How should DNA isolated on the QIAsymphony be stored?
FAQ ID -2165
View
How should samples be stored before purification using the Virus Blood 200 Protocol on the QIAsymphony?
FAQ ID -2157
View
How pure should the chloroform used with the RNeasy Microarray Tissue Mini Kit be?
FAQ ID -2193
View
Why should I use the RNeasy Microarray Tissue Mini Kit when purifying RNA for microarray analysis?
FAQ ID -2188
View
Which is the best condition for eluting GST-tagged proteins from Glutathione Superflow?
FAQ ID -2173
View
Which elution volumes can be selected using the QIAsymphony Virus Blood 200 protocol?
FAQ ID -2160
View
What sample volumes can be used with the Virus Blood 200 Protocol on the QIAsymphony?
FAQ ID -2156
View
Which plate formats are offered for QuantiTect Primer Assay Plates?
FAQ ID -2106
View
What species are QuantiTect Primer Assay Plates offered for?
FAQ ID -2110
View
Why are the denaturation and annealing/extension times for QuantiFast Multiplex RT-PCR Kits much shorter than those for QuantiFast Multiplex PCR Kits?
FAQ ID -2147
View
Which sample types can be processed using the QIAsymphony Virus Blood 200 protocol?
FAQ ID -2154
View
How many reactions can be performed per well of the QuantiTect Primer Assay Plates?
FAQ ID -2107
View
Can I use the RNeasy Microarray Tissue Mini Kit for all tissue types?
FAQ ID -2190
View
Can assays for 20 and 100 reactions be ordered on the same QuantiTect Primer Assay Plate?
FAQ ID -2108
View
Can Glutathione Superflow resin be reused?
FAQ ID -2171
View
Can Uracil-N-Glycosylase (UNG) be added to the QuantiTect PCR Master Mix and be stored for later use?
FAQ ID -2138
View
Can QuantiTect Primer Assay Plates be ordered offline via e-mail or fax?
FAQ ID -2112
View
Are there any changes in the procedure using the Strep-Tactin Superflow Plus resin with the higher capacity over the standard matrix?
FAQ ID -2168
View
Can Uracil-N-Glycosylase (UNG) be purchased separately, independent of QuantiTect PCR Kits?
FAQ ID -2134
View
Are probes other than TaqMan® probes, such as FRET probes, compatible with fast cycling with the Rotor-Gene Multiplex PCR Kit?
FAQ ID -2130
View
Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with the Rotor-Gene Multiplex PCR Kit?
FAQ ID -2131
View
Can the Rotor-Gene Multiplex RT-PCR Kit be used on other cyclers?
FAQ ID -2142
View
Can Uracil-N-Glycosylase (UNG) be used with a one-step RT-PCR kit, such as the QuantiTect Probe RT-PCR Kit?
FAQ ID -2135
View
Can RNA isolated with the RNeasy Microarray Tissue Mini Kit also be used in downstream applications other than microarray analysis?
FAQ ID -2191
View
Are Rotor-Gene Kits compatible with reaction setup using the QIAgility instrument?
FAQ ID -2116
View
Are QuantiTect PCR +UNG Kits available in bulk format (e.g., 25 ml master mix)?
FAQ ID -2136
View
Are the layouts for both 96- and 384-well QuantiTect Primer Assay Plates visible when ordering online?
FAQ ID -2111
View
Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with Rotor-Gene Probe Kits?
FAQ ID -2126
View
How does template quality influence the results with the Type-it HRM PCR Kit?
FAQ ID -2197
View
How does magnetic-particle carry-over in QIAsymphony eluates need to be handled?
FAQ ID -2166
View
Do you have protocols for software setup on various PCR platforms using QuantiFast Probe RT-PCR Kits?
FAQ ID -2105
View
How does the RNeasy Microarray Tissue Mini Kit prevent traces of phenol from negatively affecting the reverse-transcription step in microarray experiments?
FAQ ID -2189
View
How do Rotor-Gene Probe Kits compare with QuantiFast Probe Kits?
FAQ ID -2125
View
How many elution steps are recommended when using Glutathione Superflow resin?
FAQ ID -2175
View
How is the RNeasy Microarray Tissue Mini Kit different from the classic RNeasy Mini Kit?
FAQ ID -2192
View
Do you have a protocol for Rotor-Gene software setup for the Rotor-Gene SYBR Green PCR and RT-PCR Kits?
FAQ ID -2104
View
Does carrier RNA have to be added using the QIAsymphony Virus Blood 200 Protocol?
FAQ ID -2163
View
Does an internal control have to be used with the QIAsymphony Virus Blood 200 Protocol?
FAQ ID -2162
View
Can Vapor-Lock be used with samples processed on the QIAgility?
FAQ ID -2153
View
Do QuantiTect Primer Assays also work with Rotor-Gene SYBR Green PCR Kits?
FAQ ID -2123
View
Do the master mixes in Rotor-Gene Kits contain dUTP to allow UNG pretreatment?
FAQ ID -2117
View
Do limiting primer concentrations need to be determined when using the Rotor-Gene Multiplex PCR Kit?
FAQ ID -2128
View
What main input voltage is required for the QIAxcel Analyzer?
FAQ ID -1821
View
What is the minimum sample volume required for the QIAxcel System and QIAxcel Advanced?
FAQ ID -1852
View
Is there a quality difference between CompactPrep Plasmid Mega and Giga and the CompactPrep Plasmid Midi and Maxi Kits?
FAQ ID -1882
View
The Gel Cartridge is installed and all status indicators for the QIAxcel System are good but when clicking the "RUN" button, nothing happens. The green highlight goes down the Sequence?
FAQ ID -1850
View
Why does the copy function not apply relative to migration time and normalized area values after loading the DNA size marker table and adding the alignment marker peaks on the QIAxcel System?
FAQ ID -1832
View
What can be done if lower and upper alignment markers used with the QIAxcel System or QIAxcel Advanced are not aligned correctly?
FAQ ID -1834
View
Why are there no peaks in all channels during a run with the QIAxcel System or QIAxcel Advanced??
FAQ ID -1847
View
Why do DNA samples appear in only a few channels when the alignment marker is working using the QIAxcel System or QIAxcel Advanced?
FAQ ID -1844
View
Why does the Power LED not function with the QIAxcel or QIAxcel Advanced instrument plugged in and the main power switch turned on?
FAQ ID -1851
View
Can a QIAxcel DNA Fast Analysis Cartridge be run on a QIAxcel System?
FAQ ID -1828
View
Can customers use their own DNA size marker with the QIAxcel System and if yes, what concentration should be used?
FAQ ID -1841
View
Can data be saved at once with the QIAxcel System after analysis of all samples in a 96-well PCR plate?
FAQ ID -1861
View
How can a positive threshold be set when running the QIAxcel System?
FAQ ID -1843
View
How many intensity calibration runs can be performed per Gel Cartridge on the QIAxcel System?
FAQ ID -1827
View
How long will the intensity calibration procedure on the QIAxcel System take?
FAQ ID -1826
View
Why does an intensity calibration of the gel cartridge for the QIAxcel System and QIAxcel Advanced need to be performed?
FAQ ID -1824
View
Will the QIAxcel System run using a green Data Review Software Key?
FAQ ID -1853
View
Why is 'NO COM' displayed on the QIAxcel Instrument Panel although the power is on and all connections are made to the QIAxcel instrument and computer?
FAQ ID -1848
View
Why is the alignment marker for the 1.8 kb fragment not visible when using the 25 bp/1.8 kb DNA Size Marker together with the 15 bp/400 bp marker on the QIAxcel System and QIAxcel Advanced?
FAQ ID -1835
View
When running RNA samples using the QIAxcel System or QIAxcel Advanced, are there any special things to consider?
FAQ ID -1842
View
What to do if alignment marker and samples are migrating too slowly and the upper alignment marker does not appear on the gel- and/or electropherogram view with the QIAxcel System or QIAxcel Advanced?
FAQ ID -1833
View
Why are PCR bands relatively weak and "smearing" but the alignment marker bands are sharp when running samples on the QIAxcel System and QIAxcel Advanced?
FAQ ID -1837
View
When should an intensity calibration be re-performed on the QIAxcel System?
FAQ ID -1825
View
When should the N2 bottle used with the QIAxcel System be changed?
FAQ ID -1830
View
Can the QIAxcel software be loaded to another PC to view the data generated with the QIAxcel System?
FAQ ID -1855
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Can the QIAxcel System or QIAxcel Advanced be connected to a network?
FAQ ID -1862
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How can a clogged capillary channel be identified when running samples on the QIAxcel System or QIAxcel Advanced?
FAQ ID -1836
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Can multiple QIAxcel Systems be run from one computer?
FAQ ID -1860
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If a different DNA size and/or alignment marker is to be used with the QIAxcel System or QIAxcel Advanced, does the Gel Cartridge need to be recalibrated?
FAQ ID -1858
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If a different method is used with the QIAxcel System or QIAxcel Advanced, does a new reference marker table need to be created?
FAQ ID -1857
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How often should the alignment marker for the QIAxcel System be changed?
FAQ ID -1839
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How often should the solutions in the Buffer Tray for the QIAxcel System or QIAxcel Advanced be replaced?
FAQ ID -1840
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In the results table generated with the QIAxcel System, what does "normalized area" mean?
FAQ ID -1854
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How can drying out of the gel cartridge tips used on the QIAxcel System and QIAxcel Advanced be prevented?
FAQ ID -1823
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If the M500 method is used with the QIAxcel System, does a new DNA reference marker table have to be created?
FAQ ID -1856
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How can any clogged capillaries be cleared for use with the QIAxcel System and QIAxcel Advanced?
FAQ ID -1838
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What is the composition of buffer QBT?
FAQ ID -411
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What is the composition of buffer QC?
FAQ ID -412
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What is the composition of buffer P3?
FAQ ID -418
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What is the composition of buffer QF?
FAQ ID -413
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What is the composition of buffer TE?
FAQ ID -416
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What is the composition of buffer QN?
FAQ ID -414
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What is the composition of buffer OCD?
FAQ ID -423
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What is the composition of buffer FWB2?
FAQ ID -417
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What is the composition of buffer OBB?
FAQ ID -421
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What is the composition of buffer OW2?
FAQ ID -419
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What is the composition of buffer OL2?
FAQ ID -424
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What is the composition of buffer OCL?
FAQ ID -422
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