Application notes

Q Punch AS Instrument

• Learn about QIAGEN’s complete high-throughput workflow solution that addresses challenges in sample collection and analysis while ensuring the highest quality standards and first-pass success rates. A total of 360 samples can be processed in a single day by a single operator using this workflow.
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Effects of reduced elution volumes on the QIAGEN EZ1 Advanced XL extraction

• Learn how DNA concentration and yield can be improved when eluting into lower volumes with the EZ1 Advanced XL instrument.
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Automated purification of DNA from bones of a Bronze Age family using the BioRobot EZ1 workstation

• Discover how the BioRobot EZ1 workstation was used to genotype three individuals, comprising one family from the Lichtenstein cave, with automated purification of DNA from Bronze Age bones.
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Automated differential wash protocol using the QIAcube Connect and the EZ1 DNA Investigator Kit

• This protocol has been adapted to allow users to replace the epithelial transfer and sperm pellet wash steps with an automated protocol using the QIAcube Connect.
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Tissue source attribution using the PyroMark Q48 Autoprep System: Sperm identification in forensic casework

• Discover a method for tissue source identification by examining DNA methylation patterns and a novel workflow for sperm cell identification in casework samples.
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Automated differential wash protocol using the QIAcube Connect and the EZ1 DNA Investigator Kit

• This protocol has been adapted to allow users to replace the epithelial transfer and sperm pellet wash steps with an automated protocol using the QIAcube Connect.
• Read the application note

Tissue source attribution using the PyroMark Q48 Autoprep System: Sperm identification in forensic casework

• Discover a method for tissue source identification by examining DNA methylation patterns and a novel workflow for sperm cell identification in casework samples.
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Improved differential cell lysis and extraction using an automated protocol on the QIAcube Instrument

• This application note compares automated differential lysis and extraction on the QIAcube Instrument with two commonly used manual methods.
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Direct amplification and an accelerated workflow in skin flake isolation with the Investigator Casework GO! Kit

• Learn how the Investigator Casework GO! Kit provides a solution to increase DNA yield from skin flake samples while shortening and simplifying the laboratory process.
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Investigator Casework GO! Kit as a novel approach for contract traces and body fluids

• Discover an innovative method for the processing and analysis of typical biological fluids and touch traces from criminal events. The method is compared against an automated magnetic beads extraction method validated for routine use.
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High-throughput sample preparation for forensic casework using the Investigator STAR Lyse&Prep chemistry on the Tecan Freedom EVO automated platform

• Learn how high-throughput sample purification can be achieved on the Tecan Freedom EVO automated platform using QIAGEN’s Investigator STAR Lyse&Prep Kit. This method results in comparable performance and similar yields of PCR-ready, inhibitor-free DNA as with the QIAsymphony SP instrument, which would allow forensic casework laboratories tasked with managing ever-increasing workloads to increase their capacity.
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Improving workflow efficiency with the QIAsymphony SP Instrument and the Investigator Lyse&Spin Basket Kit

• Learn how the QIAsymphony SP Instrument, used in combination with QIAsymphony DNA Investigator chemistry and Investigator Lyse&Spin baskets, is an effective, reliable and safe extraction system for various types of crime scene samples.
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Verification of virtual standards with the Investigator Quantiplex Pro Kit on the QuantStudio 5 Real-Time PCR System

• Learn how virtual standards can be used with QIAGEN Investigator Quantiplex Pro Kit chemistry on the Applied Biosystems QuantStudio 5 Real-Time PCR System for reliable and precise DNA quantification.
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Assessment of Y chromosome degradation level using the Investigator Quantiplex Pro RGQ Kit

• Learn how the QIAGEN Investigator Quantiplex Pro RGQ Kit not only allows measurement of autosomal DNA degradation, but also Y chromosome degradation. This quantification kit can be applied to the analysis of casework samples extracted from bone, prior to autosomal STR and Y-STR typing.
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Evaluation of the Investigator Quantiplex Pro RGQ Kit for DNA quantification and quality assessment of forensic casework samples

• Learn how the Investigator Quantiplex Pro RGQ Kit can be used for analyzing DNA mixtures, inhibition detection and assessment of both male and human DNA degradation.
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Comparison of four commercial qPCR kits for analyzing inhibited and degraded forensic samples

• Discover the differences between four different qPCR kits for analyzing high molecular weight DNA, low template samples, degraded samples and DNA spiked with various inhibitors.
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Comparison and optimization of two qPCR DNA quantification assays to improve STR analysis workflow efficiency

• Learn how the French Gendarmerie compared two commercially available qPCR-based quantification assays to determine the optimal protocol for DNA quantification prior to STR analysis.
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Noninvasive prenatal paternity testing by means of SNP-based targeted sequencing barcoded with Unique Molecular Indices

• Learn how SNPs can be analyzed from maternal cfDNA for paternity testing. By using small numbers of SNPs and barcoding them with Unique Molecular Indices (UMIs) as part of a QIAseq targeted sequencing panel, this approach provides greater accuracy to the final sequencing data.
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Evaluation of QIAGEN’s universal next generation sequencing workflow and SNP panel including the Ion PGM system for identity and relationship testing

• Learn about QIAGEN’s NGS SNP panel; a preliminary evaluation of the 140 SNP panel as part of a workflow including the Ion PGM System demonstrated high coverage and sensitivity as well as reproducible results across a range of sample types and upstream sample prep methods.
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Development of an Individual Identity SNP panel and workflow for paternity and forensic applications

• Discover how QIAGEN developed a SNP  enrichment  panel,  along  with NGS library  prep  kits  and  bioinformatics software,  to enable  the  development  of a streamlined and focused workflow for the generation and analysis of SNP data in complex paternity cases.
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Validation of the PyroMark Q48 Autoprep compared with the PyroMark Q24 system for methylation based age estimation

• Learn how an age prediction algorithm developed on the PyroMark Q24 platform can be used for methylation data obtained using the PyroMark Q48 Autoprep without any modifications.
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Tissue source attribution using the PyroMark Q48 Autoprep System: Sperm identification in forensic casework

• Discover a method for tissue source identification by examining DNA methylation patterns and a novel workflow for sperm cell identification in casework samples.
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Compilation and investigation of Australian Aboriginal X-STR haplotypes using the Investigator Argus X-12 QS Kit

• Discover how the Investigator Argus X-12 QS Kit was used for the generation of an Australian Aboriginal X-STR population database, consisting of 298 male haplotypes.
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QIAamplifier 96 compatibility testing with Investigator 24plex QS and Investigator 24plex GO! Kits

• Explore the compatibility of the QIAamplifer 96 with HID STR amplification chemistry.
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The STAR Q Swab AS Instrument for high-throughput PCR setup of QIAGEN’s Investigator STR GO! Kits

• Learn how the OmniSwab and STAR Q workflow, used in combination with Investigator STR GO! Kits, can be used as an effective and easily adopted solution for laboratories looking to automate their manual process or increase throughput for reference and database samples.
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Increase analysis efficiency and rework of low-quality samples with the automated Quality Sensor flagging in GeneMarker HID software

• Learn how GeneMarker software provides additional time savings and avoids potential human bias when evaluating samples that have unacceptable peak heights or decreasing peak height.
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The effectiveness of STR Quality Sensors to inform rework strategies and improve STR success of challenging samples

• Learn how QS markers can be used as a straightforward, consistent and valuable tool to assist in the interpretation of STR profiles from challenging samples.
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Comparison of four commercial qPCR kits for analyzing inhibited and degraded forensic samples

• Discover the differences between four different qPCR kits for analyzing high molecular weight DNA, low template samples, degraded samples and DNA spiked with various inhibitors.
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High-throughput assay setup for the Investigator 24plex GO! Kit from Bode Buccal 2 Assembled Cassette samples using the Hamilton easyPunch

• Discover an optimized workflow for the preparation of database samples for downstream DNA profiling using STR analysis. The workflow incorporates easy to use sample collection devices ensuring reproducible and error-free collection of DNA, automation for reliable sample manipulation, imaging and punching of these collection cassettes while ensuring full sample tracking and continuity and a DNA profiling assay optimized for direct amplification from database samples.
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Elute FTA cards as a collection device for SARS-CoV-2 saliva samples

• Discover the first evaluation of FTA cards as a collection device for SARS-CoV-2 saliva samples. Using the workflow described here, FTA cards gave high RNA recovery, RNA stability and PCR sensitivity for detection of SARS-CoV-2.
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