New! Versatile kit for 3’ RNA-seq and complete transcriptome RNA-seq
Gene expression analysis can be challenging due to complex RNA-seq workflows that only offer low-throughput capabilities and demand high sample input amounts. RNA-seq resources and time are often wasted due to rRNA contamination, limiting the ability to capture on-target reads. Navigate these challenges with the help of the QIAseq UPXome RNA Library Kit – a versatile library prep solution for both 3’ RNA-seq and complete transcriptome RNA-seq with low-input and poor-quality RNA samples. Effortlessly increase throughput while reducing waste by >90% with ultraplex pooling of cDNA.
Ultralow input. Ultraplexing. On-target reads.
You no longer need two separate kits to carry out 3’ RNA-seq and complete transcriptome RNA-seq. QIAseq UPXome RNA Library Kits include protocols for library prep for both, starting with total RNA or enriched mRNA. Get the most out of limited RNA amounts and easily increase throughput to maximize efficiency and productivity on Illumina MiSeq, NextSeq and NovaSeq NGS instruments.
Seamless RNA-seq data analysis at your fingertips
Seamlessly go from generating RNA-seq data to capturing gene expression insights. QIAseq UPXome RNA Library Kits include complimentary access to the GeneGlobe RNA-seq Analysis Portal for supported species, enabling differential gene expression insights with ease .
No robot? No problem.
Increase efficiency and productivity with ultraplexing, which gives each sample a unique sample barcode and the availability of a lot of sample indices. These features allow you to have a faster workflow and process higher numbers of samples on Illumina NGS instruments. No need for automation to accelerate your workflow and ramp up throughput.
One kit, twice the functionality
Expand your pool of applications, customize your workflow and experience ultimate flexibility with this kit. Whether you’re just interested in 3’ RNA-seq or complete transcriptome RNA-seq, achieve high-quality data for applications such as:
- Gene expression analysis
- Transcript identification
- Fusion gene discovery
QIAseq UPXome RNA Library Kits vs. QIAseq UPX 3’ Transcriptome Kits
Wondering how the QIAseq UPXome RNA Library Kits differ from QIAseq UPX 3’ Transcriptome Kits? Take a look at the key differences.
| Kit name | QIAseq UPXome RNA Library Kits | QIAseq UPX 3’ Transcriptome Kits |
|---|---|---|
| Type of libraries | Both 3’ RNA-seq and complete transcriptome RNA-seq possible using a single kit |
Only intended for 3’ RNA-seq |
| Input amounts | Works with 500 pg to 100 ng of total RNA or enriched mRNA |
Works with single cells, cell lysate or less than 10 ng total RNA |
| Total workflow time | Efficient, 6-hour library prep workflow | Longer workflow |
| Ribosomal RNA removal | Integrated QIAseq FastSelect technology eliminates mitochondrial and cytoplasmic ribosomal RNA |
Does not include ribosomal RNA removal |
| Number of samples that can be ultraplexed |
Ultraplex pooling of cDNA means pooling of 768—18,432 samples per flow-cell lane and a reduction of waste by >90% |
UPX tagging allows 4608—18,432 samples per single sequencing lane |
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