Product FAQ

 

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What single cell isolation methods are recommended for use with the miScript Single Cell qPCR Kit?
FAQ ID - 3582
How would I provide information on the QuantiTect Primer assays in publications when the sequence is confidential?
FAQ ID - 3593
Is clot detection possible on the QIAsymphony?
FAQ - ID 3588
How can I determine if a tube is suitable for clot detection on QIAsymphony?
FAQ ID - 3590
In which tubes is clot detection possible on QIAsymphony?

FAQ ID - 3589

Which barcodes on sample tubes are accepted by the QIAsymphony SP?
FAQ ID - 3598
How can I create a report/logfile on my QIAsymphony SP system?
FAQ ID - 3594
Is it possible to measure the proteolytic activity of proteins on the surface of exosomes that were isolated with ExoEasy?
FAQ ID - 3599
Can I change the language on my QIAsymphony SP system?
FAQ ID - 3595
Can I use blood, cells or bacteria with the QIAamp Fast DNA Tissue Kit?
FAQ ID - 3587
Can the Rotor-Gene Q be connected to LIMS?
FAQ ID - 3596
Can the REPLI-g Mitochondrial DNA Kit be used for species other than humans?
FAQ ID - 3584
What is MOI?
FAQ ID -2768
What is RNA interference (RNAi)?
FAQ ID -2755
What is the best approach for determining where to set the CT threshold when you have >15 samples? Is it best to go through all of them, looking for a range of best fit, and then just choose one value that fits all of them?
FAQ ID -2705
What file format and layout do I need to upload my data into the PCR Array Data Analysis software?
FAQ ID -2700
What are the key factors for success in an RNAi experiment?
FAQ ID -2758
What does the RT² qPCR Primer Assay product information mean when it says that it recognizes another transcript of the same gene?
FAQ ID -2712
What do I need in order to set up an RNAi experiment?
FAQ ID -2757
What do I need to complete a RT² qPCR Primer Assay?
FAQ ID -2707
What are the SureSilencing shRNA Plasmids?
FAQ ID -2784
What criteria should one use in choosing between siRNA versus shRNA for their studies?
FAQ ID -2771
What does a typical EpiTect Methyl qPCR Assay or Array include?
FAQ ID -2740
Why did the real-time PCR yield Ct values < 12?
FAQ ID -2727
Why can't I find the DNA methylation qPCR Assay for my gene of interest?
FAQ ID -2743
What is the composition of Buffer RPE?
FAQ ID -2797
Why do I need to identify my real-time instrument model when placing my order for RT² qPCR Primer Assays?
FAQ ID -2713
What would happen if I used home-made PCR master mixes or master mixes from other manufacturers with the RT² products?
FAQ ID -2715
Which qPCR instrument should I use with your RT² qPCR Primer Assays?
FAQ ID -2714
Why are the RT² qPCR Primer Assays not designed to cross exon-intron junctions or boundaries?
FAQ ID -2710
Why should I use RT² SYBR Green Mastermix with RT² qPCR Primer Assays?
FAQ ID -2706
Will pipetting error affect the EpiTect Methyl qPCR Array results?
FAQ ID -2741
Will pipetting error affect the miRNA qPCR Assay results?
FAQ ID -2728
Why had my RT² SYBR Green Mastermix been working well in the past, but now does not seem to be?
FAQ ID -2717
What is the composition of Buffer S3?
FAQ ID -2788
What MOI should I use for my cells?
FAQ ID -2770
What RT² qPCR Primer Assays are available?
FAQ ID -2708
What transfection reagents are compatible with the Cignal Reporter Assays?
FAQ ID -2764
What is the most reliable transfection reagent for delivering shRNA plasmids and siRNA to cells in culture?
FAQ ID -2777
What is included in the SureSilencing shRNA Plasmids shipment?
FAQ ID -2785
What is the composition of Buffer RLC?
FAQ ID -2795
What is the composition of Buffer RLT?
FAQ ID -2793
What is the composition of Buffer RLT plus?
FAQ ID -2794
What is the composition of Buffer BB?
FAQ ID -2790
What is the composition of Buffer ETR?
FAQ ID -2789
What is the composition of Buffer PB?
FAQ ID -2791
What are the key parameters contributing to unwanted off-target effects in an RNAi experiment?
FAQ ID -2775
What are the structures of the siRNA molecules used in RNAi studies?
FAQ ID -2760
What are the critical factors in designing the siRNA molecules to be used for RNAi studies?
FAQ ID -2759
Why is my EpiTect Methyl qPCR Assay "failed" as indicated in the QC page of the data analysis Excel file?
FAQ ID -2734
Why is the qRT-PCR reproducibility so critical when detecting gene expression knock down in an RNAi experiment?
FAQ ID -2774
What is the composition of gDNA Eliminator Solution?
FAQ ID -2799
What is the composition of Buffer RW1?
FAQ ID -2796
What is the minimum amount of genomic DNA required for analysis using EpiTect Methyl qPCR Arrays?
FAQ ID -2739
What is the composition of Buffer RWT?
FAQ ID -2798
Can miScript miRNA qPCR Assays be used for pre-miRNA detection?
FAQ ID -2724
Can miScript miRNA qPCR Assays detect single nucleotide difference between miRNAs?
FAQ ID -2723
Do I need an internal methylated DNA reference in the EpiTect Methyl qPCR Array?
FAQ ID -2736
Do I need the sequence specific miRNA primers in the RT step of the miRNA qPCR Assays?
FAQ ID -2725
Do you always run samples in triplicates?
FAQ ID -2703
Can you use stained (H & E) sections with RT² PreAMP cDNA Synthesis Kit?
FAQ ID -2722
Does QIAGEN guarantee the performance of the SureSilencing shRNA Plasmids?
FAQ ID -2787
Does this method with the EpiTect Methyl qPCR Array detect methylation at specific CpG dinucleotides?
FAQ ID -2737
Can I make stable cell lines with the Cignal Reporter Assays?
FAQ ID -2763
Is it acceptable to correct for transfection efficiency, when determining the level of gene expression knock down by an shRNA expression vector?
FAQ ID -2783
How are the primers in the EpiTect Methyl qPCR Array designed?
FAQ ID -2735
On which instrumentation will the RT² Profiler PCR Array work?
FAQ ID -2719
Should I use monoclonal or polyclonal antibodies in EpiTect ChIP assays?
FAQ ID -2747
What are the advantages of using the SureSilencing shRNA Plasmids over siRNA?
FAQ ID -2786
The EpiTect ChIP qPCR primers I used show very high Ct. Are there any solutions?
FAQ ID -2750
The pathway reporter luciferase activity values are greater than the positive control, is there a problem?
FAQ ID -2766
The pathway reporter luciferase activity values are less than the negative control, is there a problem?
FAQ ID -2767
How can I prevent the evaporation of reaction volume from the wells EpiTect Methyl qPCR Arrays?
FAQ ID -2742
How can I prevent the evaporation of reaction volume from the wells in the miScript miRNA PCR array?
FAQ ID -2729
How can RNA interference be used as a life science research tool?
FAQ ID -2756
How can I be sure that the restriction enzyme digestion is complete in the EpiTect Methyl qPCR system?
FAQ ID -2731
How can I identify the binding sites of my transcription factor using ChIP?
FAQ ID -2753
How can I normalize my EpiTect ChIP results?
FAQ ID -2751
What is the recommended amount of input template for each RT² qPCR Primer Assay?
FAQ ID -2716
What is the RT² Profiler PCR Array?
FAQ ID -2718
Can the EpiTect Methyl qPCR System technology reliably characterize heterogenous tissue samples?
FAQ ID -2732
Can users insert custom transcriptional regulatory elements or promoters into the Cignal Reporter Assays?
FAQ ID -2765
Can you custom design the Epitect qPCR primers for the CpG island outside of the defined promoter region?
FAQ ID -2744
Can I manually set the threshold line?
FAQ ID -2702
Can I use your EpiTect One-Day kit with transcription factors?
FAQ ID -2752
Are EpiTect Methyl qPCR Arrays or Assays applicable to FFPE samples?
FAQ ID -2733
Can I obtain the sequence of the RT² qPCR Primer Assay that I purchased?
FAQ ID -2709
Are there any unique elements that need to be incorporated into an shRNA expression vector?
FAQ ID -2772
Can I use total RNA for the miRNA PCR Arrays or Assays?
FAQ ID -2726
At what point can I stop and freeze my samples when using ChIP?
FAQ ID -2754
Can I use the default PCR program to run EpiTect Methyl qPCR Arrays or Assays?
FAQ ID -2745
Can I use your EpiTect ChIP system in a RNA study?
FAQ ID -2748
Can I transform the Cignal Reporter Assays?
FAQ ID -2762
Can I reliably detect intermediately methylated DNA with EpiTect Methyl qPCR Array technology?
FAQ ID -2738
How many experiments can I do with the Cignal Lenti Reporter Assays?
FAQ ID -2769
How many housekeeping genes are included in each PCR Array?
FAQ ID -2704
Can I use fluorescence microscopy to assess shRNA plasmid transfection efficiency in my model cell line of interest, when using a vector expressing a GFP reporter gene?
FAQ ID -2782
What are the advantages of EpiTect ChIP qPCR Assays and Arrays?
FAQ ID -2721
How reliable are the results from the miScript Primer Assays?
FAQ ID -2730
What are EpiTect ChIP qPCR Assays?
FAQ ID -2720
How do you determine the efficiency using the PCR array?
FAQ ID -2701
How do I choose EpiTect ChIP-grade antibodies?
FAQ ID -2746
How do you design your EpiTect ChIP qPCR Assay primers? Do they work with SB GR?
FAQ ID -2749
How does QIAGEN control the quality of the RT² qPCR Primer Assays?
FAQ ID -2711
How does stable transfection of a shRNA-encoding plasmid work?
FAQ ID -2779
How do I choose between the DNA-based Cignal Reporter Assays and the Cignal Lenti Reporter Assays?
FAQ ID -2761
What are the storage conditions for the Microbial DNA qPCR products?
FAQ ID — 3394
Which Microbial qPCR Mastermix should I use?
FAQ ID — 3395
What is the expected amplicon size of the Microbial DNA qPCR Assays?
FAQ ID — 3396
3326 - Which QIAcube standard protocol might be suitable to extract RNA from saliva or from a buccal cell pellet?
3327 - How often should the o-ring for the pipettor tip-adapter be changed on the QIAcube?
3328 - Can the Sample Tubes CB and the Sample Tubes RB be used interchangeably?
3329 - When using REPLI-g, if the starting material is a single sorted cell, could mitochondrial DNA (mtDNA) be amplified?
3330 - Is there any contamination from E. coli genomic DNA in the polymerase provided with the Repli-G Single Cell Kit?
3331 - Should the REPLI-g amplified mtDNA be cleaned up before sequencing?
3332 - Is there a method that can distinguish between non-amplified DNA and REPLI-g amplified DNA?
3333 - When using REPLI-g, what is the purpose of the DTT added to the denaturation buffer in the cells/blood protocol?
What sample types can be tested on the arrays/assays?
FAQ ID — 3399
What is the difference between Positive PCR Control (PPC) and Microbial DNA Positive Control?
FAQ ID — 3397
Can I use the Microbial DNA-Free Water and Microbial qPCR Mastermix if they have been opened more than 3 times?
FAQ ID — 3398
What are the minimum sample requirements for Microbial DNA qPCR kits?
FAQ ID — 3393
What is the QIAxcel QX RNA Size Marker 200-6000nt?

FAQ ID - 3365

What is the nature of the gel in the QIAxcel RNA QC kit v2.0? Is it denaturing?
FAQ ID - 3366
3352 - What are the size ranges of Ni-NTA particles?
FAQ ID - 3352
3353 - What is the composition of the elution buffer in the Ni-NTA Fast Start Kit?
FAQ ID - 3353
3351 - What is the upper limit for protein size that can bind to Ni-NTA agarose resin?
FAQ ID - 3351
3354 - What type and amount of resin is packed into the Ni-NTA Spin columns from the Ni-NTA Spin Kit?
FAQ ID - 3354
3341 - How are the EpiTect DNA controls produced and tested?
FAQ - 3341
3344 - What is the difference between the EpiTect Plus DNA Bisulfite kit compared to the classic kit?
FAQ - 3344
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