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Can the QIAprep Spin Miniprep Kit be used for isolating plasmid DNA from mammalian cells?
FAQ ID -795
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Can the QIAvac 96 vacuum manifold be used with DirectPrep 96 plates?
FAQ ID -722
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Are the buffers in the Ni-NTA Fast Start Kit the same as the ones for use with Ni-NTA purchased separately?
FAQ ID -791
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Are the protocols from the BioSprint software compatible with the KingFisher software and vice versa?
FAQ ID -770
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Are Buffer PB of the QIAquick PCR Purification Kit and Buffer QG of the QIAquick Gel Extraction Kit interchangeable?
FAQ ID -786
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Any data on the fidelity of the REPLI-g MDA technique?
FAQ ID -707
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Are Centromeres and Telomeres amplified using REPLI-g WGA?
FAQ ID -702
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Can I use my own primers for REPLI-g WGA to amplify a specific chromosomal region?
FAQ ID -712
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Can I use REPLI-g for SNP Genotyping?
FAQ ID -700
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Can I do 4-plex real-time PCR, on the ABI PRISM 7000, 7700, or 7900 using the QuantiTect Multiplex PCR Kit?
FAQ ID -716
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Can I purchase Phi29 DNA polymerase only?
FAQ ID -708
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How can I get specific information and a quote for my individual Sequencing Service needs?
FAQ ID -792
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How can one perform multiplex real-time PCR analysis using the LightCycler 2.0?
FAQ ID -780
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How can I be sure that I am harvesting my induced bacterial culture at the best time point for protein expression?
FAQ ID -788
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How can I get a certificate for quality assurance (ISO 13485 and ISO 9001) from QIAGEN?
FAQ ID -762
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How do the FastLane Cell cDNA Kit and QuantiTect Reverse Transcription Kit eliminate genomic DNA contamination?
FAQ ID -783
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How many cells can be used with the FastLane Cell cDNA Kit?
FAQ ID -796
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Do you have an antibody for Western Blot Analysis of MAPK1 gene knockdown using your MAPK1 control siRNA?
FAQ ID -746
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Do I have to switch off the laser of the LiquiChip Workstation overnight?
FAQ ID -736
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Can frozen blood be used for whole genome amplification with REPLI-g?
FAQ ID -794
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How many reporter dyes can be used in the same analysis using the LiquiChip Workstation?
FAQ ID -738
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How much DNA is obtained in the average PCR reaction?
FAQ ID -750
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Do I need to determine limiting primer concentrations with the QuantiTect Multiplex PCR Kits?
FAQ ID -714
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Do I need to have EDTA in the buffer in which I am going to store my isolated genomic DNA?
FAQ ID -754
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Do you synthesize thiophosphate modified random hexamers?
FAQ ID -711
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Has anyone verified whole genome amplification accuracy with Sequencing?
FAQ ID -701
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How best to handle the light sensitive LiquiChip reagents?
FAQ ID -735
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Do you have a protocol for the fixation of cells transfected with fluorescently labeled siRNA?
FAQ ID -793
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Do you have information about the cleanup of single-stranded DNA (ssDNA) with QIAquick columns?
FAQ ID -759
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Do you have information on the use of recombinant DNA and RNA as absolute standards for realtime RT-PCR?
FAQ ID -729
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Do you have a protocol for the isolation of genomic DNA from paraffin-embedded samples on the BioRobot M48?
FAQ ID -1019
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Is the TurboCapture Microplate Holder Type B compatible with the Biomek FX robotic workstation?
FAQ ID -1040
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How can I prevent running out of M48 Kit reagents before the full number of samples are processed?
FAQ ID -1042
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What are the additional plasmid bands I see on my gel?
FAQ ID -1059
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Is it possible to scale up QuantiTect Reverse Transcription reactions to allow use of larger amounts of RNA?
FAQ ID -1063
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Is it possible to use the PhosphoProtein Purification Kit in batch format?
FAQ ID -1069
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What are the recommended storage conditions for the QuantiTect Reverse Transcription Kit and its components?
FAQ ID -1077
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What is the difference between Buffers RLT and RLT Plus?
FAQ ID -1043
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What is the difference between TurboCapture Microplate Holders Type A and Type B?
FAQ ID -1038
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Is there a limit to the size of proteins that can be purified using the Qproteome Glycoprotein Kits?
FAQ ID -1071
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What blocking reagents do you recommend for chemiluminescent detection using Penta-, Tetra-, and RGS-His antibodies?
FAQ ID -1093
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Is Factor SB of the HotStar HiFidelity Polymerase Kit the same as Factor MP of the QIAGEN Multiplex PCR Kit?
FAQ ID -1049
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How can I transfect siRNA into insect cells such as Drosophila melanogaster S2?
FAQ ID -1046
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How can I tell TurboCapture Microplate Holder Type A from Type B?
FAQ ID -1039
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How much template can I use in the reaction and what is the maximum volume of template that can be used in the QuantiTect Kits?
FAQ ID -1086
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I am seeing a precipitate after adding LyseBlue reagent to Buffer P1. What should I do about that?
FAQ ID -1045
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How much dye-labeled siRNA should I use to monitor transfection efficiency when using HiPerfect Transfection Reagent?
FAQ ID -1058
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What is the error rate of HotStar HiFidelity DNA Polymerase?
FAQ ID -1048
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How do I perform an Acetone Precipitation for concentrating and desalting protein samples?
FAQ ID -1035
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Which kit should I use for RNA isolation from Cartilage?
FAQ ID -1026
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Which QIAGEN kit do you recommend for purifying plasmid DNA suitable for transfection of sensitive cells?
FAQ ID -1092
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Where can I find the serial number on QIAGEN's BioRobots?
FAQ ID -1064
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Which kit can be used for simultaneous purification of DNA and RNA from biopsies?
FAQ ID -1089
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Why does my PCR product show up later when comparing the QuantiTect SYBR Green PCR Kits with Roche kits using the same annealing temperature?
FAQ ID -1083
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Why is there RNA in the DNA eluate when using the AllPrep DNA Mini Spin Column?
FAQ ID -1044
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Will the "classic" RNeasy Mini Kit be discontinued after the launch of the Allprep DNA/RNA Mini Kit?
FAQ ID -1054
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Why does TA/UA cloning work with your proofreading HotStar HiFidelity DNA Polymerase?
FAQ ID -1053
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Why do melting temperatures differ between PCR fragments amplified with QIAGEN's QuantiTect SYBR Green PCR Kits and Roche Kits?
FAQ ID -1084
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Where can I find a protocol for cleanup of already purified plasmid DNA?
FAQ ID -1031
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What is the protein loading capacity per column of the Qproteome Albumin/IgG Depletion Kit?
FAQ ID -1090
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What is the largest PCR amplicon that can be amplified with the HotStar HiFidelity Polymerase Kit?
FAQ ID -1047
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What is the range of melting temperatures for the QuantiTect Primer Assay amplicons in the dissociation curve?
FAQ ID -1079
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What is the recipe for 1x PBS solution?
FAQ ID -1030
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What should I use as a standard for absolute quantification in real-time PCR?
FAQ ID -1085
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What is your recommendation for cleaning the TissueLyser Bead Dispenser (96-well format)?
FAQ ID -1055
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What is the resin in the Qproteome Murine Albumin and Qproteome Albumin/IgG Depletion Kits?
FAQ ID -1034
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What is the volume of the eluate when using either spin or vacuum procedures with the QIAprep 96 Turbo Miniprep Kit?
FAQ ID -1067
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How can I prevent clogging of QIAfilter cartridges?
FAQ ID -1060
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Do I have to discard Buffer RLT with beta-Mercaptoethanol (ß-ME) added to it after 1 month of storage?
FAQ ID -1037
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Do I have to use a ramp time of 2°C/sec on the LightCycler when using Quantitect Primer Assays?
FAQ ID -1065
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Does an activation time of 15 minutes influence the performance of the HotStarTaq Plus DNA Polymerase?
FAQ ID -1050
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Do I have to use CoralLoad Gel loading dye when using your HotStarTaq Plus DNA Polymerase?
FAQ ID -1052
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Do you have a protocol for the isolation of RNA from bacterial cultures using RNAprotect Bacteria Reagent and QIAzol Lysis Reagent?
FAQ ID -1020
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Do you have a protocol for the isolation of DNA from forensic samples using the BioRobot M48?
FAQ ID -1017
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Can the USB Port of the BioSprint 96 instrument be used to transfer programs from a PC to the instrument?
FAQ ID -1032
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Do you have a protocol for the isolation of DNA from mouse tails using MagAttract technology on BioRobot M96?
FAQ ID -1007
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Are your FlexiTube siRNAs preferentially designed to any particular region of the target gene?
FAQ ID -1091
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Are the CoralLoad dyes in the HotStarTaq Plus PCR Buffer visible when loading small amounts of PCR product onto a gel?
FAQ ID -1051
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Are the new EZ1 RNA Kits (cat. no. 958034; 959034; 956034) compatible with the previous EZ1 RNA Card (cat. no. 9015590) or EZ1 RNA Universal Tissue Card (cat. no 9016384)?
FAQ ID -1025
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Can PCR fragments generated with the EasyXpress Linear Template Kit be cloned into the pIX4.0 vector of the EasyXpress Protein Synthesis Insect Kit?
FAQ ID -1078
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Can the QProteome O-Glycan Glycoprotein Kits be used for blood cells?
FAQ ID -1036
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Can the Reverse Transcriptases of the QuantiTect Reverse Transcription Kit and the QuantiTect Probe RT-PCR Kit be used interchangeably?
FAQ ID -1066
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Can the AllPrep RNA/Protein Kit be used with tissue?
FAQ ID -1095
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Can the Blocking Reagent supplied with the Penta-His HRP Conjugate be used with other antibodies?
FAQ ID -1073
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Do you recommend 1-step or 2-step real-time RT-PCR for gene expression analysis?
FAQ ID -1056
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How can I check for purity of RNA isolated using RNeasy Kits?
FAQ ID -1023
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How can air bubbles in PhosphoProtein Purification Columns be avoided?
FAQ ID -1070
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How can I increase low-copy plasmid DNA yields using QIAfilter Plasmid Kits?
FAQ ID -1062
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How can I increase DNA concentration using QIAprecipitators of the HiSpeed Plasmid Kits?
FAQ ID -1061
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How can I check the integrity of RNA purified using RNeasy Kits?
FAQ ID -1024
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How can I ensure complete genomic DNA removal when using the RNase-Free DNase Set?
FAQ ID -1087
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Do you have information on the reproducibility of DNA yields from blood using the M48 BioRobot?
FAQ ID - 1004
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Do you have a protocol for transient transfection of HeLa-S3 cells in 60 mm dishes using PolyFect?
FAQ ID -1012
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Do you have a protocol for transient transfection of HEK 293 cells in 75 cm3 flasks?
FAQ ID -1014
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Do you have a protocol for transient transfection of cells in 96-well plates using PolyFect Transfection Reagent?
FAQ ID -1013
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Do you have a protocol for the isolation of total nucleic acids from cells on the M48 BioRobot?
FAQ ID -1009
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Do you have a protocol for the isolation of total nucleic acids from tissue on the M48 BioRobot?
FAQ ID -1008
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Do you have a protocol for vacuum processing of blood samples with the QIAamp DNA Blood Midi or Maxi Kits?
FAQ ID -1011
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Do you have information on realtime RT-PCR performance of RNA isolated from monocytes on the M48 BioRobot?
FAQ ID -1010
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Do you have information on PCR template preparation from preserved blood samples on the M48 BioRobot?
FAQ ID - 1003
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Do you have information on DNA yields from blood with variable numbers of white blood cells using the M48 BioRobot?
FAQ ID -1006
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What Disinfectant Solution do you recommend for the BioRobot MDx?
FAQ ID -809
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What data documentation does QIAGEN's Sequencing Service provide for customers?
FAQ ID -832
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What are the sequences of the FlexiTube siRNAs?
FAQ ID -851
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What annealing temperature should be used with the QuantiTect Primer Assays?
FAQ ID -849
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What is the proper way to turn on or turn off the EZ1 instrument?
FAQ ID -870
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My BioRobot 9604 came with several Accessory Applications. Is it recommended I run these protocols?
FAQ ID -872
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What is the Energy Mix in the EasyXpress Kits?
FAQ ID -846
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What is the concentration of the primers in a reaction using the QuantiTect Primer Assays?
FAQ ID -850
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I would like to sequence my siRNA constructs. Does QIAGEN offer Sequencing Services for cloned siRNA?
FAQ ID -829
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Is it possible to perform immunoprecipitation (IP) directly on protein fractions from the Qproteome Cell Compartment Kit?
FAQ ID -855
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Is it possible to use frozen cell pellets with the FastLane Cell cDNA Kit?
FAQ ID -835
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Is it possible to elute plasmid DNA from the QIAprep Spin Miniprep columns with buffer containing Potassium Phosphate?
FAQ ID -854
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Is it possible to adjust the Lysis Buffer volume used with the Qproteome Bacterial Protein Preparation Kit?
FAQ ID -841
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Is it possible to use QIAGEN's pQE-TriSystem Vectors with the EasyXpress Protein Synthesis Insect Kit?
FAQ ID -876
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Is there a stopping point in the protocol for Suspended Cells using the FastLane Cell cDNA Kit?
FAQ ID -837
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Is the random biotin labeling of proteins expressed with your EasyXpress Biotin Labeling Kit as sensitive as labeling with radioactivity?
FAQ ID -875
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Is the E. coli Host Strain M15[pREP4] resistant to Zeocin?
FAQ ID -842
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Will acetone precipitation recommended in the Qproteome Protocols denature Protein?
FAQ ID -807
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Will biotin detection assays be efficient when doing in vitro translation from E. coli lysates with the EasyXpress Site-Specific Biotin Kit?
FAQ ID -878
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Why would clumps occur following the addition of Buffer P2 when using LyseBlue Reagent in a plasmid preparation?
FAQ ID -862
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Why is it recommended to add 1 mM IPTG for optimal protein yields using the EasyXpress Protein Synthesis Kits?
FAQ ID -860
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With LyseBlue reagent for lysis control, can I now process more bacterial culture and overload the columns?
FAQ ID -864
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Will site-specific biotin labeling with the EasyXpress Site-Specific Biotin kit alter the structure and function of my protein?
FAQ ID -877
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Will the sequences of the QuantiTect Primer Assays be provided?
FAQ ID -804
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Which resin is used in the QIAexpress Ni-NTA Fast Start Columns?
FAQ ID -836
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What to do if cell clumps are present after Buffer P2 addition when using LyseBlue Reagent?
FAQ ID -861
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Will the FastLane Cell cDNA Kit work with suspension cells?
FAQ ID -801
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What publicly funded Genome Sequencing Projects has QIAGEN's Sequencing Service been involved in?
FAQ ID -815
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What is the typical yield of site-specific labeled protein generated using the EasyXpress Site-Specific Biotin Kit?
FAQ ID -873
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What programs does your Sequencing Service use to ensure accurate base calling, Sequence Quality, and Alignment?
FAQ ID -831
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Where can I find a copy of the report file generated by running 'Maintenance Protocol I' on my BioRobot MDx?
FAQ ID -806
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Which QIAGEN plasmid preparation kits will contain LyseBlue Reagent?
FAQ ID -865
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Which fraction will contain soluble mitochondrial proteins using the Qproteome Cell Compartment Kit?
FAQ ID -839
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Which fraction will contain endosomal, microsomal and lysosomal proteins using the Qproteome Cell Compartment Kit?
FAQ ID -808
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Do I need to use RNase inhibitors with the RNeasy Kits?
FAQ ID -813
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Where can I find QIAGEN products for a specific gene or gene product?
FAQ ID -803
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Do the Buffers CE1 - CE4 of the Qproteome Cell Compartment Kit contain DTT or any reducing agent?
FAQ ID -824
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Can the FastLane Cell cDNA Kit also be used for qualitative end-point PCR?
FAQ ID -834
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Can the QuantiTect Primer Assays be used for detection of genomic DNA?
FAQ ID -811
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Can the FastLane Cell cDNA Kit be used with tissue?
FAQ ID -821
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Do you have a protocol for the isolation of bacteriophage P1 derived constructs with any of your plasmid kits?
FAQ ID -883
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Do you have an in vitro translation kit allowing the incorporation of seleno-methionine for X-Ray crystallography based protein analysis?
FAQ ID -879
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895 - Do you have a protocol for the isolation of endotoxin-free plasmid DNA using the QIAGEN Plasmid Midi Kit?
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Do you have a protocol for isolation of plasmid DNA using the Sigma Centrifuge 4?
-15
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Do you have a protocol for the isolation of BAC DNA using the QIAGEN Plasmid Midi Kit?
FAQ ID -881
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Can I reuse the Ni-NTA Agarose and Ni-NTA Superflow resins?
FAQ ID -802
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Can Buffer R1 of the R.E.A.L. Prep 96 Plasmid Kit be replaced with Buffer P1?
FAQ ID -823
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Are there specific recommendations for performing RT-PCR on RNA isolated from paraffin-embedded samples?
FAQ ID -828
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Can I stabilize adipose tissue using RNAprotect prior to RNA isolation?
FAQ ID -856
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Can I use the PhosphoProtein Purification Kit for the isolation of phosphorylated proteins from yeast?
FAQ ID -838
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Can I use QIAGEN's pQE vectors as templates in the EasyXpress Biotin Labeling Kits?
FAQ ID -874
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Can I use LyseBlue with R.E.A.L. Prep 96, QIAwell Ultra, or QIAprep 96 Turbo Miniprep Kits for the BioRobot systems?
FAQ ID -863
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Can I use my gene-specific primers with the FastLane Cell cDNA Kit and the QuantiTect Reverse Transcription Kit?
FAQ ID -812
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Have you tested the effect of inhibitors on PCR performance?
FAQ ID -818
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Do you sell the ATP-Dependent Exonuclease of the QIAGEN Large-Construct Kit separately?
FAQ ID -825
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How can I separate PCR fragments that are small and very close in size on an agarose gel?
FAQ ID -800
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Do you have information on in vivo RNA interference experiments?
FAQ ID -817
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I cannot see the LCD display fonts on the Sigma Centrifuge. How do I adjust the contrast?
FAQ ID -822
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I left Buffer P1 at room temperature after addition of RNase A, what shall I do?
FAQ ID -859
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How long does a typical in vitro translation reaction take with your large-scale EasyXpress protein synthesis kits?
FAQ ID -880
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How much protein can be produced with the EasyXpress Protein Synthesis Mega Kit?
FAQ ID -843
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Do you have a protocol for the isolation of very low-copy plasmids from Streptomyces spp.?
FAQ ID -893
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Do you have a protocol for the isolation of plasmid DNA from Citrobacter freundii?
FAQ ID -885
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Do you have a protocol for the isolation of plasmid DNA from Corynebacterium glutamicum?
FAQ ID -886
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Do you have a protocol for the isolation of plasmid DNA from Agrobacterium?
FAQ ID -898
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Do you have a protocol for the isolation of plasmid DNA from Borrelia spp.?
FAQ ID -884
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Do you have a protocol for the isolation of plasmid DNA from Bacillus subtilis?
FAQ ID -896
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Do you have a protocol for the isolation of plasmid DNA from yeast?
FAQ ID -853
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Do you have a protocol for the isolation of plasmid DNA from Lactobacillus spp.?
FAQ ID -887
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Do you have a protocol for the isolation of single-stranded DNA from M13 phage using QIAGEN Plasmid Kits?
FAQ ID -894
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Do you have a protocol for the isolation of plasmid DNA from Staphylococcus spp.?
FAQ ID -890
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Do you have a protocol for the isolation of plasmid DNA from Oligotropha carboxidovorans?
FAQ ID -888
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Do you have a protocol for the isolation of plasmid DNA from Proteus spp.?
FAQ ID -889
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What are the most commonly used protease inhibitors?
FAQ ID -53
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How should I quantify RNA isolated with RNeasy Kits?
FAQ ID -32
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Is plasmid DNA purified with QIAGEN Plasmid Purification Kits suitable for in vitro transcription?
FAQ ID -1
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How should I propagate pQE expression plasmids?
FAQ ID -58
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What are the compatibilities of different reagents with Ni-NTA matrices?
FAQ ID -49
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How is "Touchdown PCR" used to increase PCR specificity?
FAQ ID -75
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What should the starting template DNA quality and quantity be for PCR?
FAQ ID -74
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Why do I get smeared PCR products?
FAQ ID -87
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Why does my isolated RNA have a low OD 260/280 ratio?
FAQ ID -97
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When is chloramphenicol amplification of plasmids performed?
FAQ ID -3
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How can I remove imidazole from a protein sample?
FAQ ID -91
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How can I increase the amount of soluble recombinant protein in E. coli expression?
FAQ ID -64
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How can I avoid little or no RNA yields when using an RNeasy Kit?
FAQ ID -28
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What are the restriction sites of the pDrive Vector in the QIAGEN PCR Cloning Kit?
FAQ ID -160
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What are the molecular weights of proteins in the 6xHis Protein Ladder?
FAQ ID -169
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What are your recommendations for cotransfecting several plasmids?
FAQ ID -124
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What is the composition of Buffer EB?
FAQ ID -199
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Is mRNA isolation necessary for sensitive RT-PCR?
FAQ ID -111
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What are the recommended culture and buffer volumes for a very low-copy plasmid?
FAQ ID -168
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Should I use Ni-NTA Agarose in column or batch format for purification of 6xHis-tagged proteins?
FAQ ID -147
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What is the difference between disruption and homogenization in the RNeasy System?
FAQ ID -139
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Which Anti-His Antibody is the most sensitive for my protein of interest?
FAQ ID -172
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When should carrier be used with the QIAamp DNA Mini or the DNeasy Blood & Tissue Kit?
FAQ ID -100
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Why do I have to add beta-mercaptoethanol (beta-ME) to lysis Buffer RLT of the RNeasy Kits?
FAQ ID -101
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What is the recommended culture medium for the QIAprep System?
FAQ ID -154
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What is the RNase A concentration and composition of Buffer P1?
FAQ ID -198
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What is the genotype of the EZ Competent Cells?
FAQ ID -157
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What kind of PCR products can be cloned with the QIAGEN PCR Cloning Kit?
FAQ ID -165
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What is the principle behind Effectene Transfection Reagent?
FAQ ID -184
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What is the size of genomic DNA that is obtained with QIAGEN Genomic-tips?
FAQ ID -142
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How can I eliminate contaminating protein in my Ni-NTA 6xHis-tag protein purification?
FAQ ID -102
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How can I purify very small amounts of 6xHis-tagged protein using Ni-NTA technology?
FAQ ID -134
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How can I improve recoveries when using the QIAquick Kits?
FAQ ID -180
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How can I extract DNA from a polyacrylamide (PAGE) gel?
FAQ ID -120
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How can I improve ligation efficiency of DNA from a QIAEX II Gel Extraction Kit?
FAQ ID -141
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How can I isolate RNA from 1 gram of plant tissue?
FAQ ID -128
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How can I improve the performance of the HiSpeed QIAprecipitator module?
FAQ ID -144
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How can I improve transfection efficiency using Effectene Transfection Reagent?
FAQ ID -181
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What can I use to isolate RNA smaller than 200 nucleotides?
FAQ ID -115
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What epitopes do the Anti-His Conjugates and Anti-His Antibodies recognize?
FAQ ID -170
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What are the maximum culture volumes to use with the QIAGEN Plasmid Midi or Maxi Kit?
FAQ ID -167
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How should RNeasy Kits be stored and how long are they stable?
FAQ ID -103
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Is it possible to cleave the 6xHis-tag from an expressed protein?
FAQ ID -140
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How should I store plant material for DNA isolation using the DNeasy Plant Kit?
FAQ ID -114
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How should QIAGEN Plasmid Purification Kits be stored and for how long?
FAQ ID -192
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What are the features and benefits of the QIAexpress 6xHis Tag System?
FAQ ID -193
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What is the small band below my fragment of interest on an agarose gel after DNA cleanup using QIAquick?
FAQ ID -148
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Do you have transfection data for QIAGEN Transfection Reagents?
FAQ ID -158
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Do I need to use an RNase inhibitor in my RT reaction?
FAQ ID -119
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Do you have data showing effects of sample size on DNA yield and purity using the DNeasy 96 Blood & Tissue kit?
FAQ ID -129
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How can I avoid variable transfection efficiencies with SuperFect Reagent?
FAQ ID -179
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Does QIAGEN offer vectors for direct cloning of PCR products?
FAQ ID -146
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Can a QIAquick Gel Extraction Kit be used to obtain RNA from a formaldehyde gel?
FAQ ID -133
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Are QIAamp DNA isolation kits suitable for apoptosis studies?
FAQ ID -149
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Can DyeEx be used to clean up labeled DNA other than sequencing templates?
FAQ ID -137
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Are RNeasy spin columns sold separately?
FAQ ID -159
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Can I use QIAprep Miniprep kits for low-copy plasmids and cosmids?
FAQ ID -127
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Can I use the QIAquick PCR Purification Kit for restriction enzyme cleanup?
FAQ ID -130
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Can I use a QIAfilter Cartridge for purifying large plasmids?
FAQ ID -121
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Can I use Omniscript or Sensiscript RT's at a higher temperature?
FAQ ID -116
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How does imidazole affect my quantitation of protein?
FAQ ID -132
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How can I optimize the transfection of oligos and large plasmids using PolyFect Transfection Reagent?
FAQ ID -176
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How do you ensure that RNeasy buffers are RNase-free?
FAQ ID -113
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How can I purify DNA from soil, food and sewage samples for PCR?
FAQ ID -118
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How can I obtain DNA-free RNA using an RNeasy Midi or Maxi Kit?
FAQ ID -143
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How do I prepare an insert for pQE vectors?
FAQ ID -185
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How should I store Anti·His and Tag·100 Antibodies?
FAQ ID -194
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Do you have a protocol for purification of total DNA from insects?
FAQ ID -1254
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Is your Nuclease-Free Water fluorescence-free?
FAQ ID -1291
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Do you have a protocol for purification of total DNA from crude lysates?
FAQ ID -1255
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How should the GelPilot DNA Loading Dye be handled?
FAQ ID -1287
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What quality level does your Nuclease-Free Water have?
FAQ ID -1292
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What is the pH value of QIAGEN's Nuclease-Free Water?
FAQ ID -1290
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Does GelPilot DNA Loading Dye contain ethidium bromide?
FAQ ID -1286
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Are MaXtract tubes siliconized?
FAQ ID -1298
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Can phenol/chloroform be placed in the MaXtract tube for extraction at a later time?
FAQ ID -1299
Visualizar
Should I input the dilution factor of my samples into the LiquiChip Analyser Software dilution factors of samples?
FAQ ID -1211
Visualizar
What cell lines have been tested with the Qproteome Plasma Membrane Protein Kit?
FAQ ID -1205
Visualizar
What are the new features of the EasyXpress Insect Kit II compared to the original Protein Synthesis Insect kit?
FAQ ID -1220
Visualizar
What are your recommendations for PCR template preparation for use with the EasyXpress Insect Kit II?
FAQ ID -1221
Visualizar
Is it possible to increase nucleic acid concentrations when following protocols on the BioRobot EZ1?
FAQ ID -1232
Visualizar
Is a saliva collection device provided in the RNeasy Protect Saliva Mini Kit?
FAQ ID -1213
Visualizar
How should I prepare buffy coat samples for use on the BioRobot EZ1?
FAQ ID -1249
Visualizar
Is it possible to use QIAGEN's pQE-Trisystem Vector with the EasyXpress Insect Kit II?
FAQ ID -1225
Visualizar
Is training of lab personnel included with the purchase of a BioRobot EZ1?
FAQ ID -1239
Visualizar
Where can I find information about the worktable setup on the BioRobot EZ1?
FAQ ID -1243
Visualizar
Is RNAprotect Cell Reagent provided in the RNeasy Protect Cell Kit available separately?
FAQ ID -1217
Visualizar
What vector do you recommend as template for in-vitro protein expression with the EasyXpress Insect Kit II?
FAQ ID -1224
Visualizar
What type of promoter should my template have for in-vitro protein expression with the EasyXpress Insect Kit II?
FAQ ID -1223
Visualizar
What is the difference between various FlexiTube siRNAs listed for the same target gene, and which one should I choose?
FAQ ID -1206
Visualizar
Will the Qproteome Plasma Membrane Protein Kit work with starting materials other than adherent mammalian cells?
FAQ ID -1202
Visualizar
What disposables are required for nucleic acid isolations on the BioRobot EZ1?
FAQ ID -1246
Visualizar
What is the protein yield when using the Qproteome Plasma Membrane Protein Kit?
FAQ ID -1201
Visualizar
What is the maximum number of cells that can be used with the AllPrep RNA/Protein Kit ?
FAQ ID -1208
Visualizar
Do the foils sealing the EZ1 Reagent Cartridges have to be manually removed prior to loading the robot?
FAQ ID -1240
Visualizar
What is the smallest and the largest protein that you tested for expression with the EasyXpress Insect Kit II?
FAQ ID -1218
Visualizar
What is the size of the BioRobot EZ1?
FAQ ID -1245
Visualizar
Does anything have to be added to express post-translational modifications (PTMs) with the EasyXpress Insect Kit II?
FAQ ID -1226
Visualizar
Do you have a protocol for isolating cytoplasmic RNA from animal cells using the RNeasy Mini Kit?
FAQ ID -1207
Visualizar
Do you have a protocol for purification of miRNA from animal cells using the RNeasy Plus Mini Kit and RNeasy MinElute Cleanup Kit?
FAQ ID -1258
Visualizar
Do you have a protocol for purification of cytoplasmic RNA from animal cells?
FAQ ID -1257
Visualizar
Can cells stabilized with RNAprotect Cell Reagent be used for cell sorting by flow cytometry (FACS)?
FAQ ID -1216
Visualizar
Can glycosylated proteins be expressed using the EasyXpress Insect Kit II?
FAQ ID -1227
Visualizar
Can the Strep-Tactin Magnetic Beads of the Qproteome Plasma Membrane Protein Kit be reused?
FAQ ID -1204
Visualizar
Can RNAprotect Saliva Reagent be purchased separately?
FAQ ID -1214
Visualizar
Can you recommend a Reagent or Kit for stabilizing RNA in cultured cells?
FAQ ID -1215
Visualizar
Can the RLT lysate of the BioSprint DNA Plant protocol be stored frozen?
FAQ ID -1248
Visualizar
Can the Qproteome Plasma Membrane Protein Kit be used with tissue samples?
FAQ ID -1200
Visualizar
How can we get our BioRobot EZ1 upgraded when new protocols are launched?
FAQ ID -1241
Visualizar
How can I decontaminate the BioRobot EZ1?
FAQ ID -1231
Visualizar
How much active protein can be expressed in-vitro using the EasyXpress Insect Kit II, and how much time does it take?
FAQ ID -1222
Visualizar
How do you ensure that concentrated RNA is purified from saliva using the RNeasy Protect Saliva Mini Kit?
FAQ ID -1212
Visualizar
Do you have a protocol for purification of total DNA from yeast?
FAQ ID -1253
Visualizar
How many freeze-thaw cycles can the various reaction mixes in the EasyXpress Insect Kit II tolerate?
FAQ ID -1219
Visualizar
Do you have a protocol for the isolation of DNA from buccal cells using the BioRobot EZ1?
FAQ ID -1260
Visualizar
Do you have a protocol for transfection of suspension cell lines (Jurkat and K562) with siRNA?
FAQ ID -1250
Visualizar
How are plasma membrane containing vesicles eluted off the ligand in the Qproteome Plasma Membrane Protein procedure?
FAQ ID -1203
Visualizar
Do you have a protocol for transfection of differentiated macrophage cell lines (THP) with siRNA?
-1
Visualizar
Do you have a protocol for transfection of macrophage cell lines (J774.A1 and RAW 264.7) with siRNA?
FAQ ID -1251
Visualizar
Can I use a centrifuge instead of vacuum when using the QIAquick 96 PCR Purification Kit?
FAQ ID -293
Visualizar
If I want to use BioMag particles, do I have to buy a QIAGEN magnet too or can I use one that I already have?
FAQ ID -266
Visualizar
Can the PAXgene Blood RNA Tubes undergo freeze/thaw cycles?
FAQ ID -2471
Visualizar
Should I use Omniscript or Sensiscript for reverse transcription of low-copy mRNA?
FAQ ID -297
Visualizar
I used to buy BioMag particles from a company by another name? Are your BioMag particles the same thing?
FAQ ID -263
Visualizar
What are the main differences between other magnetic beads and the BioMag particles?
FAQ ID -272
Visualizar
My BioMag is about to expire, is it still any good?
FAQ ID -268
Visualizar
If I am using BioMag Streptavidin to capture biotinylated oligos, how can I then remove the oligos?
FAQ ID -279
Visualizar
How magnetically responsive is BioMag?
FAQ ID -265
Visualizar
What is the recipe for LB?
FAQ ID -212
Visualizar
What is the maximum binding capacity of RNeasy spin columns?
FAQ ID -290
Visualizar
What is the recipe for 2x YT?
FAQ ID -213
Visualizar
Why does my DNA sample float out of the slot when loading it onto an agarose gel?
FAQ ID -205
Visualizar
Why is an ice-incubation step included during reaction set-up when following the QuantiTect RT-PCR but not the QuantiTect PCR protocol.
FAQ ID -283
Visualizar
What sequencing primers can I use with the pDrive cloning vector of the QIAGEN PCR Cloning Kit?
FAQ ID -292
Visualizar
What is the composition of Buffer P2?
FAQ ID -203
Visualizar
What is the difference between the QIAamp MinElute Virus Spin and the MinElute Virus Vacuum Kit?
FAQ ID -295
Visualizar
What is the composition of the QIAGEN Multiplex PCR Buffer?
FAQ ID -289
Visualizar
What is the difference between the Fc specific versions of your secondary antibody BioMag particles and the non-specific?
FAQ ID -275
Visualizar
Can I use HEPES buffer instead of phosphate in my Ni-NTA column?
FAQ ID -291
Visualizar
Can I use my own cycling conditions with the QIAGEN Multiplex PCR Kit?
FAQ ID -287
Visualizar
Can I select FITC labeled cells with BioMag?
FAQ ID -278
Visualizar
A white precipitate has formed in my 10x RT buffer. Is it still ok to use?
FAQ ID -216
Visualizar
Can I use BioMag particles with any specific primary antibody?
FAQ ID -273
Visualizar
Can I clean up my DNase treated RNA samples using RNeasy columns?
FAQ ID -286
Visualizar
Are BioMag particles stable in other solvents?
FAQ ID -267
Visualizar
Can BioMag be used for positive and negative selection?
FAQ ID -269
Visualizar
How can I avoid poor immunolocalization morphology with Anti-His Antibodies?
FAQ ID -200
Visualizar
For how long are BioMag Particles stable?
FAQ ID -262
Visualizar
How can I prevent non-specific binding when using BioMag?
FAQ ID -277
Visualizar
How do I prevent bubbles from forming in my Ni-NTA agarose column?
FAQ ID -285
Visualizar
How can I purify mRNA from total RNA using an Oligotex Direct mRNA Kit?
FAQ ID -201
Visualizar
How can one determine the optimal annealing temperature for a specific PCR assay?
FAQ ID -288
Visualizar
Does QIAGEN sell Q-Solution separately?
FAQ ID -204
Visualizar
What is the composition of Protein Precipitation Solution?
FAQ ID -2810
Visualizar
What is the composition and concentration of Glycogen Solution in Gentra Puregene Kits?
FAQ ID -2812
Visualizar
What is the composition of Buffer RDD?
FAQ ID -2800
Visualizar
What is the composition of Cell Lysis Solution?
FAQ ID -2809
Visualizar
What is the composition of DNA Hydration Solution in Gentra Puregene Kits?
FAQ ID -2813
Visualizar
What is the composition of Cell Suspension Solution?
FAQ ID -2811
Visualizar
Which heating block is recommended for the pyrosequencing annealing step?
FAQ ID -2838
Visualizar
What is the recommended amplicon size for CpG assays?
FAQ ID -2825
Visualizar
Which end of the PCR primer for pyrosequencing should be biotinylated?
FAQ ID -2839
Visualizar
Which competent E. coli cells should I use to amplify the SureSilencing shRNA Plasmids?
FAQ ID -2890
Visualizar
Which analyses can be performed with the PyroMark Q96 ID software version 1.0?
FAQ ID -2845
Visualizar
Which kits can be used in combination with the PyroMark CpG Assays and PyroMark Custom Assays?
FAQ ID -2822
Visualizar
What region of LINE gets targeted by the assay for PyroMark Q96 MD or PyroMark Q24?
-1
Visualizar
What should be the single peak height for the PyroMark Control Oligo on the different PyroMark instruments?
FAQ ID -2852
Visualizar
Does the same U1 promoter in the SureSilencing shRNA Plasmids express shRNA in human, mouse, and rat cell lines?
FAQ ID -2887
Visualizar
What is the amplicon length of the PyroMark CpG LINE assay?
-1
Visualizar
How are the PyroMark CpG Assays reconstituted?
FAQ ID -2817
Visualizar
How can I use the SureSilencing shRNA Plasmids for stable transfection and knock down in a cell line that is already resistant to both neomycin and puromycin?
FAQ ID -2895
Visualizar
Does the PyroMark LINE assay target all LINE sequences?
-1
Visualizar
Can I use fluorescence microscopy to assess the transfection efficiency of the SureSilencing shRNA Plasmids with GFP in my model cell line of interest?
FAQ ID -2894
Visualizar
Can I use my own siRNA design?
FAQ ID -2897
Visualizar
Can I reclone the insert sequences from the SureSilencing shRNA Plasmids into a viral-based vector?
FAQ ID -2896
Visualizar
Can I directly transfect SureSilencing shRNA Plasmids into my cells?
FAQ ID -2899
Visualizar
Can I order the nucleotides from PyroMark Gold Reagents separately?
FAQ ID -2827
Visualizar
Do any of the SureSilencing shRNA Plasmids contain inducible promoters?
FAQ ID -2888
Visualizar
Does QIAGEN offer a design of Custom PyroMark CpG Assays?
FAQ ID -2818
Visualizar
Can unused wells in a pyrosequencing plate be used in the next run?
FAQ ID -2872
Visualizar
Can PyroMark Gold reagents be vortexed?
FAQ ID -2844
Visualizar
Can the PyroMark Q96 CpG LINE assay be used with an ID system?
-1
Visualizar
What are the features of PyroMark CpG Assays e.g. in terms of design, validation?
FAQ ID -2821
Visualizar
How do I reduce background peaks in the pyrosequencing pyrogram?
FAQ ID -2877
Visualizar
What are the features of the new PyroMark Q96 ID software 2.5?
FAQ ID -2867
Visualizar
What are the changes of PyroMark Assay Design Software Version 2.0 compared to 1.0?
FAQ ID -2849
Visualizar
Is there a user manual available for the PyroMark Assay design software?
FAQ ID -2851
Visualizar
Should I use the SureSilencing shRNA Plasmids with the neomycin-resistance marker, the puromycin-resistance marker, or the GFP reporter gene?
FAQ ID -2882
Visualizar
What else do I need to complete an experiment using the SureSilencing shRNA Plasmids?
FAQ ID -2889
Visualizar
What cell line does QIAGEN use for quality control testing of the SureSilencing shRNA Plasmids?
FAQ ID -2885
Visualizar
What is included in a PyroMark Custom Assay?
FAQ ID -2815
Visualizar
What cell lines can I use with the SureSilencing shRNA Plasmids?
FAQ ID -2891
Visualizar
How many nucleotides of a homopolymer can be resolved in pyrosequencing?
FAQ ID -2871
Visualizar
What concentration should be used for the sequencing primer in pyrosequencing?
FAQ ID -2826
Visualizar
How many CpG sites are analyzed by the PyroMark CpG LINE assay?
-1
Visualizar
How do I set up a PyroMark CpG Assay?
FAQ ID -2814
Visualizar
How long should I wait after transfection before enriching my transfected cells, validating knock down, and looking for phenotypic changes?
FAQ ID -2893
Visualizar
How many times can the cartridges for Pyromark Q24 or PyroMark Q96 ID instruments are reused?
FAQ ID -2863
Visualizar
Is the CpG software included in the PyroMark instruments to study methylation status?
FAQ ID -2842
Visualizar
Is the expression level of miRNA precursors lower compared to that of mature miRNAs?
FAQ ID -2807
Visualizar
In which format can PyroMark CpG Assays be ordered?
FAQ ID -2824
Visualizar
How many times can the CDTs, NDTs, and RDTs be used?
FAQ ID -2865
Visualizar
How many times can vacuum troughs be re-used with the PyroMark Vacuum Preparation Stations?
FAQ ID -2848
Visualizar
What is the meaning of the abbreviations NDT and CDT and what are the differences?
FAQ ID -2841
Visualizar
What is the PyroMark Q96 Data Converter?
FAQ ID -2868
Visualizar
What is the composition of RBC Lysis Solution?
FAQ ID -2808
Visualizar
What is the concentration of PyroMark Control Oligo?
FAQ ID -2846
Visualizar
What is the pyrosequencing data exchange tool for?
FAQ ID -2864
Visualizar
What is the reason for signals ceasing in the middle of a pyrosequencing run?
FAQ ID -2875
Visualizar
What is the reason for split peaks appearing in between dispensations on my pyrosequencing pyrogram?
FAQ ID -2881
Visualizar
What is the reason for a high substrate peak in the pyrosequencing pyrogram?
FAQ ID -2879
Visualizar
What is the composition of Buffer FRN?
FAQ ID -2802
Visualizar
What is the composition of Buffer PKD?
FAQ ID -2801
Visualizar
What is the composition of Buffer APP?
FAQ ID -2804
Visualizar
What is the composition of Buffer ALO?
FAQ ID -2805
Visualizar
What is the composition of Buffer APL?
FAQ ID -2803
Visualizar
Which analyses can be performed with the PyroMark Q96 MD software?
FAQ ID -2866
Visualizar
Will dUTP in a PCR reaction affect pyrosequencing?
FAQ ID -2843
Visualizar
Will the primer sequence for the PyroMark CpG Assay be provided?
FAQ ID -2823
Visualizar
What is the use of the PyroMark Q24 Validation Oligo?
FAQ ID -2855
Visualizar
Which purity grade is recommended for pyrosequencing primers?
FAQ ID -2832
Visualizar
Which PyroMark Gold Q96 Reagent should be used for which instrument and application?
FAQ ID -2836
Visualizar
What kind of shaker should be used for the pyrosequencing binding step?
FAQ ID -2837
Visualizar
What is the sensitivity limitation for pyrosequencing?
FAQ ID -2840
Visualizar
What is the SureSilencing shRNA Plasmids guarantee?
FAQ ID -2898
Visualizar
What primers may I use to sequence the shRNA cassette within the SureSilencing shRNA Plasmids?
FAQ ID -2886
Visualizar
Where can I order the Streptavidin Sepharose beads for pyrosequencing?
FAQ ID -2850
Visualizar
Where to find explanations to the warning given by the PyroMark software after run data analysis?
FAQ ID -2874
Visualizar
Does the Pyromark Assay Design or application software give any cycling conditions for individual assay primers or a PCR setup pipetting scheme?
FAQ ID -2862
Visualizar
Does the PyroMark CpG LINE assay target mouse transposons as well?
-1
Visualizar
How are the PyroMark CpG Assays shipped and stored?
FAQ ID -2816
Visualizar
How do I find the SureSilencing shRNA Plasmids for my gene of interest?
FAQ ID -2883
Visualizar
How do I prevent a drifting baseline in my pyrosequencing pyrogram?
FAQ ID -2878
Visualizar
How are the SureSilencing shRNA Plasmids shipped?
FAQ ID -2884
Visualizar
Is the pH indicated in the formulation sheet for Screening Suite buffers the final pH?
FAQ ID -1123
Visualizar
Is the surface tension of the crystallization supports of the EasyXtal 15-Well Tools similar to that of a siliconized cover slide?
FAQ ID -1107
Visualizar
Do you have a protocol for high-throughput purification of plasmid DNA using the BioRobot Universal System?
FAQ ID -1191
Visualizar
Is it possible to collect intact mitochondria before or after the density gradient centrifugation step of the Qproteome Mitochondria Isolation protocol?
FAQ ID -1187
Visualizar
What are the applications the EasyXtal 15-Well Tool can be used for?
FAQ ID -1111
Visualizar
Is the EasyXtal Tool compatible with liquid-handling systems and visualization robots?
FAQ ID -1118
Visualizar
What EZ1 DNA Investigator protocol is recommended for very precious samples?
FAQ ID -1154
Visualizar
What cell lines have been tested with the Fastlane Cell cDNA Kit?
FAQ ID -1175
Visualizar
What does transcript variant mean when searching for specific QuantiTect Primer Assays?
FAQ ID -1137
Visualizar
What does 'QIAGEN Certified Solution' for Protein Crystallization mean?
FAQ ID -1121
Visualizar
How many protein crystallization conditions are available at QIAGEN?
FAQ ID -1119
Visualizar
How much detection reagent do you generally recommend for LiquiChip Assays?
FAQ ID -1159
Visualizar
How many LiquiChip beads are recommended for an xMAP experiment?
FAQ ID -1161
Visualizar
How many cells can be used with the Qproteome Mitochondria Isolation Kit, and how much mitochondrial protein can be expected?
FAQ ID -1189
Visualizar
How many drops can be set up on a protein crystallization support?
FAQ ID -1110
Visualizar
How much solution can I add to an EasyXtal 15-Well Tool reservoir well?
FAQ ID -1114
Visualizar
If I cannot find a QuantiTect Primer Assay for my target gene in rat, can I use the assays for the orthologous genes in mouse and human?
FAQ ID -1145
Visualizar
In which formats are crystallization condition Screening Suites available?
FAQ ID -1127
Visualizar
If a QuantiTect Primer Assay has more than one version number, does this mean that earlier assay versions may not work?
FAQ ID -1136
Visualizar
How sensitive is the LiquiChip Reader?
FAQ ID -1157
Visualizar
What is a QuantiTect Primer Assay?
FAQ ID -1141
Visualizar
How much time is needed for a LiquiChip assay point measurement?
FAQ ID -1162
Visualizar
What is the delivery time for QuantiTect Primer Assays?
FAQ ID -1142
Visualizar
What kind of strategy for initial screening of protein crystallization conditions do you recommend?
FAQ ID -1120
Visualizar
Why are there various different QuantiTect Primer Assays for my gene (previous versions, transcript variants)?
FAQ ID -1133
Visualizar
When should the EZ1 DNA Investigator Normalization protocol be used?
FAQ ID -1155
Visualizar
Where can I find the composition tables for solutions used in individual Crystallization Screening Suites?
FAQ ID -1124
Visualizar
When searching for a QuantiTect Primer Assay for my gene of interest, why do I only find the assay for human but not for rat?
FAQ ID -1139
Visualizar
Can the EZ1 DNA Investigator Tip Dance protocol cope with any solid matrix remaining in the sample tube?
FAQ ID -1153
Visualizar
What type of magnet do you recommend for handling the magnetic beads in the Qproteome Plasma Membrane Protein protocol?
FAQ ID -1193
Visualizar
What starting material can be used with the Qproteome Mitochondria Isolation Kit?
FAQ ID -1184
Visualizar
Are EasyXtal 15-Well Tools stackable?
FAQ ID -1116
Visualizar
Which QuantiTect Primer Assay should I choose for my gene of interest?
FAQ ID -1135
Visualizar
Why can I not find the QuantiTect Primer Assay in GeneGlobe that I had previously ordered from QIAGEN?
FAQ ID -1138
Visualizar
Which chemical products do you use to prepare screening solutions for protein crystallization?
FAQ ID -1122
Visualizar
Will proteins eluted in the Qproteome Plasma Membrane Protein protocol be modified, e.g. with the ligand?
FAQ ID -1195
Visualizar
Why does the QuantiTect Primer Assay for my gene of interest have only one version number?
FAQ ID -1134
Visualizar
Will the ligand in the Qproteome Plasma Membrane Protein protocol be specific for plasma membrane exclusively?
FAQ ID -1198
Visualizar
Will integral, peripheral as well as lipid-anchored plasma membrane proteins be isolated with the Qproteome Plasma Membrane Protein Kit?
FAQ ID -1199
Visualizar
Why is there no QuantiTect Primer Assay for my gene of interest?
FAQ ID -1140
Visualizar
What is the dynamic range of detection of the LiquiChip Reader?
FAQ ID -1158
Visualizar
What is the EasyXtal 15-Well Tool made of?
FAQ ID -1106
Visualizar
Can the nuclei and cell debris fraction generated in the QProteome Mitochondria isolation procedure be used for further fractionation?
FAQ ID -1185
Visualizar
Can the EZ1 DNA Forensic Card be used with the EZ1 DNA Investigator Kit?
FAQ ID -1151
Visualizar
Can the EasyXtal 15-Well Tools be reused?
FAQ ID -1117
Visualizar
Can the Qproteome Plasma Membrane Protein Kit be used with suspension cells?
FAQ ID -1194
Visualizar
Can your Tag antibody recognize rat MAPK1 also?
-100
Visualizar
Can the remaining fractions obtained in the Qproteome Plasma Membrane Protein procedure be used in downstream assays?
FAQ ID -1197
Visualizar
Can xMAP kits from other Luminex partners be used on the LiquiChip System purchased from QIAGEN?
FAQ ID -1156
Visualizar
Can the Spin Columns of the Qproteome Albumin/IgG Depletion Kit be reused?
FAQ ID -1173
Visualizar
Are protein modifications such as glycosylation problematic when using the Qproteome Plasma Membrane Protein Kit?
FAQ ID -1196
Visualizar
Are scoring sheets available for each crystallization condition Screening Suite?
FAQ ID -1125
Visualizar
Can mitochondria separated with the Qproteome Mitochondria Isolation Kit be used for downstream DNA isolation?
FAQ ID -1188
Visualizar
Can acetone be used for precipitation of protein from Buffer RLT lysates generated with RNeasy Kits?
FAQ ID -1164
Visualizar
Can I increase the annealing temperature recommended for QuantiTect Primer Assays used with the QuantiTect SYBR Green PCR Kits?
FAQ ID -1146
Visualizar
Does the EasyXtal Tool conform to an SBS format?
FAQ ID -1115
Visualizar
How are NeXtal deep-well blocks sealed? Can they be resealed?
FAQ ID -1128
Visualizar
Do you have WGA protocols for starting materials not mentioned in the REPLI-g Handbooks?
FAQ ID -1160
Visualizar
Do you have a protocol for the isolation of genomic DNA from whole blood for use in MRC-Holland MLPA® assays?
FAQ ID -1169
Visualizar
Do you have a protocol for the purification of 6xHis-tagged proteins using BioSprint?
FAQ ID -1167
Visualizar
How easy is it to scoop up a protein crystal with the lip around the crystallization supports of the EasyXtal 15-Well Tools?
FAQ ID -1113
Visualizar
How is the quality of the crystallization condition Screening Suites guaranteed?
FAQ ID -1126
Visualizar
How do I use EasyXtal Crystallization Tools?
FAQ ID -1105
Visualizar
How did you determine that your AllStar Negative Control siRNA binds to RISC?
FAQ ID -1180
Visualizar
How does the sealing efficiency of the crystallization supports of the EasyXtal 15-Well Tool compare to grease?
FAQ ID -1109
Visualizar
Do you have a kit for protein isolation from mitochondria prepared with your Qproteome Mitochondria Isolation Kit?
FAQ ID -1190
Visualizar
Do you have a kit for the isolation of RNA from formalin-fixed, paraffin-embedded samples?
FAQ ID -1174
Visualizar
Do QuantiTect Primer Assays contain SYBR Green dye?
FAQ ID -1143
Visualizar
Do I need an ultracentrifuge for the density gradient centrifugation step in the Qproteome Mitochondria Isolation protocol?
FAQ ID -1186
Visualizar
Do protein crystals stick to the walls of cavities when using the DropGuard crystallization supports?
FAQ ID -1108
Visualizar
Do you have a protocol for evaluating pipetting accuracy of the BioRobot EZ1?
FAQ ID -1131
Visualizar
Do you have a protocol for purification of DNA from stool samples for pathogen detection using BioSprint Instruments?
FAQ ID -1165
Visualizar
Do you have a protocol for purification of DNA from stool samples using BioSprint Instruments?
FAQ ID -1166
Visualizar
Do you have a protocol for evaluating temperature accuracy of the BioRobot EZ1?
FAQ ID -1168
Visualizar
How can I analyze proteins from exosomes and other extracellular vesicles (EVs) obtained with the exoEasy Maxi Kit by SDS-PAGE?
FAQ ID - 3707
Visualizar
What is the concentration of SureENTRY Transduction Reagent?
FAQ ID - 3708
Visualizar
Is it possible to modify the transduction protocol for Cignal Lenti Reporter Assays to save some pipetting steps?
FAQ ID - 3709
Visualizar
Is it possible to get a Custom RT2 Profiler PCR Array, cat. # 330131, with primer assays targeting transcripts of different species, e.g. assays targeting transcripts from mouse and assays targeting transcripts from human, on one plate?
FAQ ID - 3710
Visualizar
Can I store the QIAseq Beads of the QIAseq Targeted DNA Panel at -20°C?
FAQ ID - 3711
Visualizar
Is it possible to purchase Buffer VXL which is part of the QIAamp cador Pathogen Mini Kit, (Cat No.: 54104, 54106) separately?
FAQ ID - 3712
Visualizar
What sample tubes can I use on my QIAsymphony SP system?
FAQ ID - 3713
Visualizar
How can I decontaminate my QIAsymphony SP / AS system?
FAQ ID - 3714
Visualizar
What is the difference between the QIAamp UCP DNA Micro Kit (cat. no. 56204) and RNeasy UCP Kits (cat. no. 73934) and non-UCP QIAGEN purification kits?
FAQ ID - 3715
Visualizar
Can I use carrier RNA with the RNeasy UCP Micro Kit (cat. no. 73934)?
FAQ ID - 3716
Visualizar
Can other QIAGEN buffers from non-UCP kits (e.g. AW1 instead of AUW1) be used with the QIAamp UCP DNA Micro Kit (cat. no. 56204)?
FAQ ID - 3717
Visualizar
How is the Pyromark Q48 cartridge to be cleaned?
FAQ ID - 3718
Visualizar
Can the disc and absorber strips for the Pyromark Q48 be reused?
FAQ ID - 3720
Visualizar
What is the dead volume of the cartridges in PyroMark Q48?
FAQ ID - 3719
Visualizar
How much DNA is needed in the PyroMark Q48?
FAQ ID - 3721
Visualizar
Can I transfer my assays from PyroMark Q24/Q96 to PyroMark Q48 Autoprep?
FAQ ID - 3723
Visualizar
Can I use PyroMark Q48 for diagnostics?
FAQ ID - 3722
Visualizar
Can I analyze my Next Generation Sequencing (NGS) samples on the QIAxcel for Quality control?
FAQ ID - 3724
Visualizar
How long does an Investigator Quantiplex Pro run take?
FAQ ID - 3727
Visualizar
Can I buy the 1 ml Bead Plates from the DNeasy UltraClean 96 Microbial Kit (384) separately?
FAQ ID - 3726
Visualizar
Is an additional proteinase K digest possible with DNeasy PowerSoil Kit and DNeasy PowerLyzer Kit?
FAQ ID - 3725
Visualizar
On which real-time cyclers is the Investigator Quantiplex Pro Kit validated?
FAQ ID - 3728
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Can the Investigator Quantiplex Pro Kit be run on on the Rotor-Gene Q?
FAQ ID - 3729
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Which real-time cycler software version can be used to run the Investigator Quantiplex Pro Kit?
FAQ ID - 3730
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Do I have to calibrate new dyes on my Applied Biosystems 7500 or 7500 Fast Real-Time PCR System?
FAQ ID - 3732
Visualizar
How long can I store the dilutions of the Male Control DNA M1 standards at 8°C?
FAQ ID - 3733
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What do I have to consider if I am using the Applied Biosystems SDS software version 1.2.3 in order to run an Investigator Quantiplex Pro reaction?
FAQ ID - 3731
Visualizar
Why do I observe a CT shift for the internal control in some samples in comparison to the CT value for the internal control of the dilution series of the Male Control DNA M1?
FAQ ID - 3734
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Which human target is used for the quantification?
FAQ ID - 3736
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How long are the target sequences of Investigator Quantiplex Pro?
FAQ ID - 3735
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At what temperature should I store the Investigator Quantiplex Pro Kit?
FAQ ID - 3737
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How can I streamline the quantification workflow?
FAQ ID - 3738
Visualizar
Does QIAGEN offer an anti-GFP gapmer control?
FAQ ID - 3739
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How long does an Investigator Quantiplex Pro RGQ run take?
FAQ ID - 3740
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On which real-time cyclers is the Investigator Quantiplex PRO RGQ Kit validated?
FAQ ID - 3741
Visualizar
Which Rotor-Gene Q System can be used to run the Investigator Quantiplex Pro RGQ Kit?
FAQ ID - 3742
Visualizar
Which real-time cycler software version can be used to run the Investigator Quantiplex Pro RGQ Kit?
FAQ ID - 3743
Visualizar
How long can I store the dilutions of the Male Control DNA M1 standards at 2–8°C?
FAQ ID - 3744
Visualizar
How long are the target sequences of the Investigator Quantiplex Pro RGQ Kit?
FAQ ID - 3746
Visualizar
Why do I observe a CT shift for the Internal Control in some samples in comparison to the CT value for the Internal Control of the dilution series of the Male Control DNA M1?
FAQ ID - 3745
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Which human target is used for the quantification?
FAQ ID - 3747
Visualizar
At what temperature should I store the Investigator Quantiplex Pro RGQ Kit?
FAQ ID - 3748
Visualizar
How can I streamline the quantification workflow?
FAQ ID - 3749
Visualizar
Which fixation method is used in the PAXgene Tissue System?
FAQ ID - 3600
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What is the fixation reagent of PAXgene Tissue FIX?
FAQ ID - 3601
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What is the stabilization reagent of PAXgene Tissue STABILIZER?
FAQ ID - 3602
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What is the maximum tissue size that can be fixed in a PAXgene Tissue FIX Container (50 ml)?
FAQ ID - 3604
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Why are two reagents used in the PAXgene Tissue System?
FAQ ID - 3603
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Which conditions are recommended for the storage of tissues in PAXgene Tissue STABILIZER?
FAQ ID - 3605
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Is it possible to process formalin-fixed and PAXgene Tissue fixed samples together in one run?
FAQ ID - 3606
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Is it necessary to clean a processor normally used for formalin-fixed tissue before using it with PAXgene Tissue fixed tissue?
FAQ ID - 3607
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Is it possible to integrate the PAXgene Tissue STABILIZER into tissue processing?
FAQ ID - 3608
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Are immunohistochemistry (IHC) assays developed for formalin-fixed, paraffin-embedded (FFPE) tissues compatible with PAXgene Tissue fixed, paraffin-embedded (PFPE) tissues?
FAQ ID - 3609
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Is it possible to use formalin-fixed, paraffin-embedded (FFPE) kits and protocols to isolate biomolecules from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID - 3610
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How well is RNA integrity preserved in PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID - 3611
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How well is DNA integrity preserved in PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID - 3612
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Are special kits and protocols required to isolate biomolecules from PAXgene Tissue fixed, cryo-embedded (PFCE) tissue?
FAQ ID - 3614
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What are the yield and integrity of nucleic acids isolated from blocks of PAXgene Tissue fixed, cryo-embedded (PFCE) tissue?
FAQ ID - 3613
Visualizar
Does the PAXgene Tissue RNA Kit co-purify small RNAs?
FAQ ID - 3615
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Which kits and protocols can be used to isolate nucleic acids from microdissected PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) specimens?
FAQ ID - 3617
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Can proteins be extracted from PAXgene Tissue fixed specimens?
FAQ ID - 3616
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What is the PCR performance of DNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissue compared to DNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?
FAQ ID - 3618
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How is the quality of proteins purified from PAXgene Tissue fixed and stabilized tissue?
FAQ ID - 3619
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How do I prepare Buffer MP?
FAQ ID - 3620
Visualizar
We recently changed the OS from Windows XP to Windows 7. When re-installing software Pyromark Q24 2.0.6, it fails to analyse the results. Any suggestions?
FAQ ID - 3621
Visualizar
Can the RNeasy kits be used to extract RNA from saliva collected using the Oragene collection kit?
FAQ ID - 3623
Visualizar
How do I analyze codon 61 mutations with the RAS extension kit (cat. no. 971590)?
FAQ ID - 3622
Visualizar
Are the QIAshredder columns included in various QIAGEN kits the same?
FAQ ID - 3624
Visualizar
Do I need to use a blood collection set with the PAXgene Blood ccfDNA Tube?
FAQ ID - 3626
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Are QR codes recognized by QIAsymphony?
FAQ ID - 3625
Visualizar
Does the insert sequence provided in the specification sheet correspond to the target sequence or to anti-sense sequence?
FAQ ID - 3627
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What is the reagent in the PAXgene Blood ccfDNA Tube?
FAQ ID - 3632
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What is the shelf life of unused PAXgene Blood ccfDNA Tubes?
FAQ ID - 3633
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How should unused PAXgene Blood ccfDNA Tubes be stored?
FAQ ID - 3634
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Are PAXgene Blood ccfDNA Tubes sterile?
FAQ ID - 3636
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Are there special considerations for phlebotomy when using the PAXgene Blood ccfDNA Tube (blood collection set, discard tube, positioning of the tube, issues with short draws, etc.)?
FAQ ID - 3635
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What is the expected ccfDNA yield from plasma generated from 10 ml of blood?
FAQ ID - 3637
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How long can whole blood stabilized in PAXgene Blood ccfDNA Tubes be stored at room temperature?
FAQ ID - 3639
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Can I use plasma from underfilled PAXgene Blood ccfDNA Tubes?
FAQ ID - 3638
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Can whole blood in PAXgene Blood ccfDNA Tubes be stored at temperatures below 15°C?
FAQ ID - 3641
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Can whole blood in PAXgene Blood ccfDNA Tubes be stored at temperatures above 25°C?
FAQ ID - 3640
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Can whole blood be frozen in PAXgene Blood ccfDNA Tubes?
FAQ ID - 3642
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How long and at which temperature can plasma generated from whole blood in PAXgene Blood ccfDNA Tubes be stored?
FAQ ID - 3644
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Is double centrifugation required for plasma processing?
FAQ ID - 3643
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How should I thaw frozen plasma samples?
FAQ ID - 3645
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What should I do if cryoprecipitates form while thawing a frozen plasma sample?
FAQ ID - 3646
Visualizar
How many extractions can I perform with a QIAsymphony PAXgene Blood ccfDNA Kit, using the 2.4 ml or the 4.8 ml protocols?
FAQ ID - 3649
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Which plasma volumes can I process with the QIAsymphony PAXgene Blood ccfDNA protocols?
FAQ ID - 3648
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How long after first use can a reagent cartridge be stored for further use?
FAQ ID - 3650
Visualizar
For what type of sample material is the QIAsymphony PAXgene Blood ccfDNA Kit intended?
FAQ ID - 3652
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Which tubes are recommended for the QIAsymphony sample rack
FAQ ID - 3653
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Which protocol line should I use to process plasma samples?
FAQ ID - 3654
Visualizar
How should eluates produced with the PAXgene Blood ccfDNA System be stored?
FAQ ID - 3657
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Does the QIAsymphony PAXgene Blood ccfDNA Kit also allow eluting ccfDNA in less than 60 µl?
FAQ ID - 3656
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Which downstream applications have been tested with ccfDNA purified from whole blood collected and processed with the PAXgene Blood ccfDNA System?
FAQ ID - 3658
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What is the shelf life of the QIAsymphony PAXgene Blood ccfDNA Kit?
FAQ ID - 3651
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Which elution volumes are available with the QIAsymphony PAXgene Blood ccfDNA Kit?
FAQ ID - 3655
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Why is a blood collection set required to collect blood into the PAXgene Blood ccfDNA Tube?
FAQ ID - 3628
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Can I use a different brand blood collection set with the PAXgene Blood ccfDNA Tube?
FAQ ID - 3630
Visualizar
Can reagent from the PAXgene Blood ccfDNA Tube flow back into the patient’s arm?
FAQ ID - 3629
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What is the blood draw volume of the PAXgene Blood ccfDNA Tube
FAQ ID - 3631
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Where can I find the gene list for a specific RT² Profiler PCR Array?
FAQ ID - 3684
Visualizar
Is it possible to store nucleic acids extracted using the SpeedXtract Virus Kit?
FAQ ID - 3680
Visualizar
Can REPLI-g WGA be used to amplify cDNA if the cDNA is dsDNA (double-stranded DNA)?
FAQ ID - 3686
Visualizar
What is the difference between RT² Profiler PCR Array and RT² Profiler PCR Array PLUS version?
FAQ ID - 3685
Visualizar
I would like to stop during the library preparation procedure. When can I do that?
FAQ ID - 3687
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Should total RNA or small enriched RNA be used as the starting material for the QIAseq miRNA Library Kit?
FAQ ID - 3659
Visualizar
Total RNA from what sample types are compatible with the QIAseq miRNA Library Kit?
FAQ ID - 3660
Visualizar
Can miRNA sequencing libraries be prepared from total RNA isolated from heparinized plasma samples?
FAQ ID - 3661
Visualizar
Are any components of the QIAseq miRNA Library Kit interchangeable with components from other QIAGEN kits?
FAQ ID - 3662
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What are recommended stopping points during the QIAseq miRNA Library Kit procedure?
FAQ ID - 3663
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When multiplexing samples, what sample indexes should be combined?
FAQ ID - 3664
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What is the maximum number of available sample indexes?
FAQ ID - 3665
Visualizar
Can I still perform sequencing if my miRNA sequencing library concentrations are too low to obtain a 4 nM library?
FAQ ID - 3666
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What method is used to eliminate adapter dimers from the QIAseq miRNA library?
FAQ ID - 3667
Visualizar
Does a QIAseq miRNA library include hY4 Y RNA?
FAQ ID - 3670
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What is the recommended read length for libraries prepared using the QIAseq miRNA Library Kit?
FAQ ID - 3668
Visualizar
How do Unique Molecular Indexes (UMIs) improve quantification?
FAQ ID - 3669
Visualizar
What is the sequence of the QIAseq miRNA NGS 3’ Adapter?
FAQ ID - 3671
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What is the sequence of the QIAseq miRNA NGS 5’ Adapter?
FAQ ID - 3672
Visualizar
What is the acceptable number of reads per sample per miRNA sequencing run?
FAQ ID - 3673
Visualizar
Can you perform qPCR analysis of miRNAs from libraries prepared with the QIAseq miRNA Library Kit?
FAQ ID - 3674
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Which regions are covered by the GR DNAseq V2 Panels (#181900)?
FAQ ID - 3688
Visualizar
What can be used to validate results obtained with the QIAseq miRNA Sequencing System?
FAQ ID - 3675
Visualizar
When preparing plasma with the PAXgene Blood ccfDNA tubes, cat. # 768115, do you recommend that the first centrifugation step should be done with the brakes on or off?
FAQ ID - 3690
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Do the covered regions in the BED file include the primers?
FAQ ID - 3689
Visualizar
How do I insert and replace an absorber strip into the PyroMark Q48 Autoprep chamber?
FAQ ID - 3691
Visualizar
How much space does the PyroMark Q48 Autoprep instrument need?
FAQ ID - 3693
Visualizar
Are peak heights of Q48 comparable to other PyroMark instruments?
FAQ ID - 3692
Visualizar
Can unused wells in a pyrosequencing discs be used in the next run?
FAQ ID - 3694
Visualizar
Where can I find the certificate of calibration for the Rotor-Disc OTV Kit (catalog number 981400)?
FAQ ID - 3695
Visualizar
What is the minimum guaranteed shelf-life of the PAXgene Blood ccfDNA Tubes (100) (catalog no. 768115)?
FAQ ID - 3696
Visualizar
What do the GTIN, UDI and REF abbreviations mean on the label of any QIAGEN box?
FAQ ID - 3697
Visualizar
What methods are used during normalization of RNAseq data in the secondary GeneGlobe Data Analysis Center?
FAQ ID - 3698
Visualizar
Do eluates generated with the PAXgene Blood miRNA Kit contain residual genomic DNA?
FAQ ID - 3637
Visualizar
Can purification columns from other suppliers be processed on the QIAcube Connect?
FAQ ID - 141487
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Do I need to buy special kits for the QIAcube Connect?
FAQ ID - 141485
Visualizar
Can I program my own protocols for the QIAcube Connect?
FAQ ID - 141486
Visualizar
How can I get software updates for the QIAcube Connect?
FAQ ID - 141494
Visualizar
Can the QIAcube Connect rotor and/or buckets be removed for cleaning?
FAQ ID - 141491
Visualizar
Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube Connect?
FAQ ID - 141496
Visualizar
Do I need to discard partially used QIAcube Connect tip racks?
FAQ ID - 141499
Visualizar
How can I decontaminate the QIAcube Connect system?
FAQ ID - 141500
Visualizar
How can I load new protocols onto the QIAcube Connect?
FAQ ID - 141501
Visualizar
Can the QIAcube Connect heater/shaker be used independently from protocol runs?
FAQ ID - 141502
Visualizar
What dedicated QIAcube Connect kits are available?
FAQ ID - 141504
Visualizar
Where can I find the ordering information for QIAcube Connect accessories such as shaker rack plugs, rack labeling strips, reagent bottle racks, 30 ml reagent bottles, rotor-adapter holder, rotor adapters, sample tubes CB, sample tubes RB and spin-column adapter rings?
FAQ ID - 141505
Visualizar
Do you have a protocol for the AllPrep DNA/RNA/Protein Mini Kit on the QIAcube Connect?
FAQ ID - 141506
Visualizar
Do you have a protocol for the QIAprep Spin M13 Kit on the QIAcube Connect?
FAQ ID - 1414507
Visualizar
Which QIAcube Connect standard protocol might be suitable to extract RNA from saliva or from a buccal cell pellet?
FAQ ID - 141508
Visualizar
How often should the o-ring for the pipettor tip adapter be changed on the QIAcube Connect?
FAQ ID - 141509
Visualizar
Can the CB and RB sample tubes be used interchangeably?
FAQ ID - 141510
Visualizar
Why can I not find the Q-Base device in the device list during the first installation?
FAQ ID - 141516
Visualizar
Which network settings are supported by the QIAcube Connect and Q-Base?
FAQ ID - 141517
Visualizar
Why can’t I see my QIAcube Connect instrument in the QIAcube Connect App?
FAQ ID - 1414518
Visualizar
Why is the QIAcube Connect App reacting so slowly?
FAQ ID - 141519
Visualizar
Why are not all applications/kits/protocol files visible in the run setup (QIAcube Connect App)?
FAQ ID - 1414520
Visualizar
Why does the connection test fail?
FAQ ID - 141522
Visualizar
How critical is the time of the temperature change from 60°C to 80°C?
FAQ ID -
143768
Visualizar
Can the Repli-g Advanced DNA Single Cell Kit also be used for bacterial cells?
FAQ ID -
143079
Visualizar
Why is DTT used only for semen samples?
FAQ ID -
143761
Visualizar
Is it possible to use Lyse&Spin baskets?
FAQ ID -
143762
Visualizar
Can the lysis volume be reduced for higher sensitivity?
FAQ ID -
143763
Visualizar
What if only one heater shaker is available?
FAQ ID -
143764
Visualizar
How long are sample lysates stable in the fridge/ freezer?
FAQ ID -
143765
Visualizar
If 400 µl are used as lysis volume, how do I concentrate the lysate? (Otherwise the DNA would be too diluted.)
FAQ ID -
143766
Visualizar
Why is the QIAamp Viral RNA Mini Kit (50) currently unavailable?
FAQ ID -147395
Visualizar
Why is there no male DNA obtained from sexual assault samples?
FAQ ID -
143767
Visualizar
What RNAs has QIAseq FastSelect been designed to remove?
FAQ ID -147910
Visualizar
How does QIAseq FastSelect work?
FAQ ID -147907
Visualizar
Is QIAseq FastSelect truly as easy as combining a reagent with RNA and ramping down the temperature?
FAQ ID -147909
Visualizar
Will QIAseq FastSelect work in other species?
FAQ ID -147911
Visualizar
What library prep kits has QIAseq FastSelect been tested with?
FAQ ID -147912
Visualizar
I am using an RNA library prep kit that is not listed in the QIAseq FastSelect handbook. Will QIAseq FastSelect work with my kit?
FAQ ID -147913
Visualizar
What RNA range has the QIAseq FastSelect been tested with?
FAQ ID -147914
Visualizar
Can QIAseq FastSelect Plant or QIAseq FastSelect Yeast be combined with FastSelect 5S/16S/23S?
FAQ ID -147915
Visualizar
Is it possible to test the efficiency of FastSelect rRNA removal by using a Bioanalyzer, etc.?
FAQ ID -147916
Visualizar
How do I safely inactivate biohazardous flow-through material? 1 2
FAQ ID -12
Visualizar
What are the expected DNA yields from different tissues using the QIAamp DNA Mini Kit? 8
FAQ ID -45
Visualizar
How can I increase expression of my 6xHis-tagged protein in E. coli? 2
FAQ ID -63
Visualizar
How can I decrease ribosomal RNA (rRNA) contamination using Oligotex?
FAQ ID -642
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Do you have a protocol for purification of cytoplasmic RNA from animal cells?
FAQ ID -1257
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kemot solk - Can cDNA prepared by any method be used as starting material in the ligation reaction of the QuantiTect Whole Transcriptome Kit? test
FAQ ID -1589
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