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Do I have to use an internal control with each QIAsymphony DSP Virus/Pathogen Kit protocol run?
FAQ ID -2932
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Do samples require pretreatment before they are loaded onto the QIAsymphony instrument?
FAQ ID -2931
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Can the QIAsymphony DSP Virus/Pathogen system be used for diagnostic or prognostic procedures?
FAQ ID -2939
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Do the ELISArray Kits work on species other than human, mouse or rat?
FAQ ID -2906
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Does the QIAsymphony mericon Bacteria Kit contain an extraction control?
FAQ ID -2961
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Is it possible to simultaneously isolate DNA and RNA from Saliva Stabilized in RNAprotect® Saliva Reagent?
FAQ ID -2996
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Is it possible to use templates with signal sequences with EasyXpress Disulfide E. coli Kits?
FAQ ID -2969
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Is food DNA co-purified using the QIAsymphony mericon Bacteria Kit?
FAQ ID -2959
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Is it possible to introduce an affinity tag using the EasyXpress Linear Template Fab Kit?
FAQ ID -2972
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Is it possible to isolate DNA from Saliva Stabilized in RNAprotect® Saliva Reagent?
FAQ ID -2995
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On what cyclers have Certal Residual DNA Detection Kits been validated?
FAQ ID -2978
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On which cyclers can Certal Residual Detection Kits be used?
FAQ ID -2973
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Should I use the SureSilencing shRNA Plasmids with the GFP, neomycin, or hygromycin, or puromycin resistance marker?
FAQ ID -2900
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Is it possible to use the native signal sequence of antibodies with EasyXpress Disulfide Insect Kits?
FAQ ID -2968
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Is RNA co-purified using the QIAsymphony mericon Bacteria Kit?
FAQ ID -2960
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How much DNA does a bacterial cell contain?
FAQ ID -2947
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Is there a risk of crosstalk from the FAM channel to the VIC channel if the residual DNA target is present in very high copy numbers?
FAQ ID -2980
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How much DNA does a human cell contain?
FAQ ID -2948
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How much RNA does a bacterial cell contain?
FAQ ID -2949
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How many reactions can be performed with one QIAsymphony mericon Bacteria Kit?
FAQ ID -2955
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How many samples can be processed with the QIAsymphony DSP Virus/Pathogen Kits?
FAQ ID -2935
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How should the samples be stored before purification of RNA with the QIAsymphony PAXgene Blood RNA Protocol?
FAQ ID -2984
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How much DNA and RNA can be expected from human blood cells?
FAQ ID -2950
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How should the samples be stored before purification with the QIAsymphony DSP Virus/Pathogen Kits?
FAQ ID -2934
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If a cycler from Applied Biosystems is used in combination with the Certal Residual DNA Detection Kit, should dye calibration for MAX be performed?
FAQ ID -2977
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How much RNA does a typical mammalian cell contain?
FAQ ID -2946
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How much sample is required for each well for an ELISArray Kit?
FAQ ID -2908
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How much starting material can be used with the QIAsymphony DSP DNA Mini Kit on the QIAsymphony SP?
FAQ ID -2927
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What is the recommended solution in which to store RNA samples that will be used as templates for cDNA synthesis?
FAQ ID -2659
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What is the QIAxcel Advanced System?
FAQ ID -2650
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What is the purpose of the ROX and fluorescein dyes, also known as passive reference dyes, in qPCR master mixes?
FAQ ID -2671
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What is the optimal DNA concentration for the Investigator ESSplex Plus Kit?
FAQ ID -2646
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What negative controls are typically included in qPCR and/or qRT-PCR experiments?
FAQ ID -2672
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What is the SBTengine® software?
FAQ ID -2628
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What is the standard curve method for qPCR assay data analysis? How is the standard curve method for qPCR assay data analysis performed?
FAQ ID -2691
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What is the delta Rn value?
FAQ ID -2681
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What is the threshold cycle or Ct value?
FAQ ID -2682
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What is the comparative or ??Ct method for qPCR assay data analysis? How is the comparative or ??Ct method for qPCR assay data analysis performed?
FAQ ID -2693
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What is the key technical challenge in isolating high quality RNA from cell or tissue samples?
FAQ ID -2656
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What is qPCR?
FAQ ID -2668
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What is the difference between Absolute Quantification and Relative Quantification in qPCR, using the standard curve approach?
FAQ ID -2692
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Why do I see low, poor, or sub-standard amplification efficiency in my qRT-PCR assay?
FAQ ID -2695
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Is the QuantiFast SYBR Green PCR Kit compatible with the ViiA7 cycler from Applied Biosystems?
FAQ ID -2652
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What is the difference between adding the Internal Control (IC) template used in the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit to the amplification reaction versus adding the IC template at the extraction step?
FAQ ID -2600
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What is a dissociation curve, and why is it important to run a dissociation curve, following qPCR using SYBR Green chemistry?
FAQ ID -2678
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What are the most reliable methods for preparing high-quality RNA from cell or tissue samples, for use in gene expression analysis experiments?
FAQ ID -2657
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What are the differences between one-step and two-step RT--PCR?
FAQ ID -2666
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What are the guidelines for choosing a housekeeping gene for normalizing qPCR results?
FAQ ID -2674
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Why are my qPCR Ct values too high (> 35 or not detectable) in my qRT-PCR assay?
FAQ ID -2685
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Why are my qPCR Ct values too low (< 12) in my qRT-PCR Assay?
FAQ ID -2684
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Why is 18S ribosomal RNA (rRNA) used as a housekeeping gene to normalize sample-to-sample, systematic variation in qPCR assays?
FAQ ID -2675
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Why is my no template control (NTC) real-time Ct value < 35 cycles in my qPCR Assay?
FAQ ID -2686
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Why do the Internal Control templates for extraction (Internal Control DNA or RNA [High conc.]) have a 10x higher concentration than the IC templates provided with the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit?
FAQ ID -2603
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Why do I see multiple high-intensity peaks in my qPCR dissociation curve at temperatures less than 70 ºC?
FAQ ID -2690
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Why do my qPCR amplification curves or plots decrease in fluorescence intensity after the saturation phase?
FAQ ID -2689
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What should the cycler set-up be for a duplex reaction with the pathogen assay (FAM) and the Internal Control assay (MAX) on ABI instruments, when using the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit?
FAQ ID -2610
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What should the cycler set-up be for a duplex reaction with the pathogen assay (FAM) and the Internal Control assay (MAX) on Mx instruments from Stratagene (Agilent), when using the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit?
FAQ ID -2609
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What positive controls are typically included in qPCR and/or qRT-PCR experiments?
FAQ ID -2673
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What should I do if I suspect that my RNA preparation contains RNase contamination?
FAQ ID -2661
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What real-time cycler should I use for my qPCR experiments?
FAQ ID -2670
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Which DNA/RNA extraction kits were tested in combination with the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit?
FAQ ID -2606
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For the QuantiFast Pathogen RT-PCR +IC kit, does the Internal Control also control for the reverse transcription reaction?
FAQ ID -2601
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What should the Rotor-Gene Q cycler settings be for a duplex reaction with the pathogen assay (FAM) and the Internal Control assay (MAX), when using the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit?
FAQ ID -2608
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What testing should be performed to assess the quality of an RNA sample?
FAQ ID -2660
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Following thermal cycling, reaction volume has evaporated from some of the wells of my qPCR assay plate. What should I do?
FAQ ID -2683
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Do we have data on long term stability and storage of DNA purified on BioRobot M48?
FAQ ID -2611
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During data analysis, how should the threshold be set in the Yellow channel on the Rotor-Gene Q cycler to analyze the Internal Control from the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit
FAQ ID -2607
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How can I predict the percent qPCR signal due to contaminating DNA, for a given qPCR assay, and its matching NRT control?
FAQ ID -2688
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How can I ensure that reaction volume is not lost due to evaporation during thermal cycling?
FAQ ID -2679
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How can I determine whether amplification occurs from mRNA-derived cDNA or from genomic DNA contamination?
FAQ ID -2687
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How can I avoid or remove genomic DNA contamination from the total RNA preparation?
FAQ ID -2662
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Do the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit contain ROX in the master mix?
FAQ ID -2605
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Can the Internal Control DNA or RNA be added directly to the sample?
FAQ ID -2602
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Are primers available that only detect mitochondrial DNA encoded genes and not nuclear genomic DNA encoded genes?
FAQ ID -2680
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Do I need to run a standard curve before the actual PCR array experiment?
FAQ ID -2664
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Does the amount of Internal Control to be spiked into the lysis buffer or lysate depend on the elution volume only?
FAQ ID -2604
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May I try the data analysis tool without using your PCR array kit?
FAQ ID -2698
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What are the common primer and probe chemistries utilized for qPCR assays?
FAQ ID -2669
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Is the QuantiFast Multiplex PCR +R Kit compatible with the ViiA7 cycler from Applied Biosystems?
FAQ ID -2653
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Is the QuantiFast Probe PCR +ROX Vial Kit compatible with the ViiA7 cycler from Applied Biosystems?
FAQ ID -2651
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How do I determine the amplification efficiency of my qPCR assay?
FAQ ID -2694
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How do I determine the linear dynamic range of my qPCR or qRT-PCR assay?
FAQ ID -2696
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How do I create a workspace that is free of DNA contamination, prior to carrying out a qPCR experiment?
FAQ ID -2654
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How does HotStart PCR help minimize nonspecific amplification events?
FAQ ID -2676
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Is it good to pool multiple RNA replicates to detect expression changes that are consistently reproducible?
FAQ ID -2663
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How important is the RNA purification process, for obtaining reliable qRT-PCR results?
FAQ ID -2655
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3212 - What is the minimum size of DNA fragment that can be isolated with QIAamp DNA Mini kit?
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3210 - Can the 4-16K Centrifuge be used with a 208 V outlet?
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3211 - What is the maximum speed of the plate rotor 2 x 96 in the 4-16 or 4-16K?
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3202 - Can GeneRead rRNA depletion kit be used to deplete rRNA from miRNA isolated using miRNeasy kit?
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3299-Can Buffer ER from the Endofree Plasmid kits be used instead of the Buffer ETR in QIAGEN Plasmid Plus kits?
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3213 - How much ethanol should be added to Buffer AP3/E (renamed to Buffer AW1) and Buffer AW (renamed to Buffer AW2) in the DNeasy Plant kit?
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3200 - What is the Maximum/Minimum amount of RNA that can be used with GeneRead rRNA depletion kit?
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3201 - What quality of RNA can be used with the GeneRead rRNA depletion kit?
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3203 - Is the RNeasy MinElute cleanup kit required for the GeneRead rRNA depletion kit?
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3204 - How strict is the purification cut-off with GeneRead Size Selection kit?
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3205 - Can GeneRead Library preparation kits be used on species other than human mouse and rat, for example, plant species and microbial species?
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3206 - Can our GeneRead Library preparation kits be used with the SoLiD Platform?
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3248 - What is the difference between sponge, cubic and lamellar phase?
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3254 - When using the Nextal Cubic Phase products how do I know in which phase the sample is? What will I see?
Visualizar
3255 - When using the NexTal Cubic Phase products, should I work with diluted or undiluted screens?
Visualizar
3227 - In which solution can I keep my protein with the NexTal Cubic Phase kits?
Visualizar
3217 - How long can I store my purified RNA?
Visualizar
3225 - How pure should my protein be for the NexTal CubicPhase kits?
Visualizar
3292-How do I start using the NeXtal CubicPhase kits?
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3295-What is the difference between SBTexcellerator HLA Core, Extended and Full Kits?
Visualizar
3240 - What are the main differences between the qBiomarker PCR Arrays and the RT2 Profiler PCR Arrays?
Visualizar
3237 - I would like to order the QuantiFast Probe Assays without master mix. Is this possible?
Visualizar
3233 - Is the yield and quality of RNA and DNA purified with the AllPrep DNA/RNA FFPE Kit comparable to yield and quality of nucleic acids purified separately with other methods.
Visualizar
3265 - Why are there various different QuantiFast Probe Assays for my gene (previous versions, transcript variants)?
Visualizar
3298-Will the sequence of the QuantiFast Probe Assays be provided?
Visualizar
3262 - Which thermal cyclers are validated for use with the Investigator Nonaplex ESS Kit?
Visualizar
3263 - Why are samples centrifuged at 4°C after the addition of chloroform when using the RNeasy Plus Universal Mini Kit?
Visualizar
3264 - Why are the QuantiFast Probe Assays located on a single exon, thereby also detecting genomic DNA?
Visualizar
3241 - What are the recommended storage conditions of the QuantiFast Probe RT-PCR Plus Kit components?
Visualizar
3238 - What are important points regarding the genomic DNA wipeout buffer in the QuantiFast Probe RT-PCR Plus Kit?
Visualizar
3239 - What are the differences between the existing QuantiFast Probe RT-PCR Kit and the new Plus Kit?
Visualizar
3247 - What is the correct matrix to use with the Investigator Nonaplex ESS Kit?
Visualizar
3245 - What is the concentration of the primers and the probe in a reaction using the QuantiFast Probe Assays?
Visualizar
3243 - What does the Investigator Nonaplex ESS Kit contain?
Visualizar
3244 -What is the average length of a QuantiFast Probe Assay?
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3231 - Is there a size limit to the protein I can crystallize with the NexTal Cubic Phase products?
Visualizar
3232 - Is the RNeasy Plus Universal Mini Kit suited for adipose, brain, and other fatty tissues?
Visualizar
3228 - Is it also possible to isolate miRNA with the AllPrep DNA/RNA FFPE Kit?
Visualizar
3229 - Is it necessary to perform calibration steps for the use of the MAX dye in duplex RT-PCR experiments on different cyclers?
Visualizar
3236 - I use cultured cells as starting material. Can I also use the RNeasy Plus Universal Kit?
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Is there also a miRNeasy Plus Universal Kit available?
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3261 - Which purification kits are recommended for the therascreen Pyro kits?
Visualizar
3258 - Which filter set and settings should be used with the Investigator Nonaplex ESS Kit?
Visualizar
3260 - Which purification kits are recommended for the Pyro kits?
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3259 - Which probe labels are available for the QuantiFast Probe Assays?
Visualizar
3249 - What is the optimal DNA volume and concentration for the Investigator Nonaplex ESS Kit?
Visualizar
3296-Why does the A peak reduction factor have to de set to 0.86 for the codon 61 assay in the therascreen NRAS Pyro Kit?
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What plug-ins are available for the therascreen Pyro kits?
FAQ ID - 3251
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What plug-ins are available for the Pyrosequencing kits?
FAQ ID - 3250
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3252 - What should be the concentration of my protein with the NexTal Cubic Phase products?
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3256 - Which crystallization solutions are compatible with lipidic cubic phase crystallization?
Visualizar
3257 - Which deparaffinization method can I use with the AllPrep DNA/RNA FFPE Kit?
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3253 - What species are QuantiTect Probe Assays offered for?
Visualizar
Can the insertions in Exon 20 be detected with the therascreen EGFR Pyro Kit?
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Can the insertions in Exon 20 be detected with the EGFR Pyro Kit?
FAQ ID - 3279
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3277 - Can I use the RNeasy Plus Universal Mini Kit for all types of tissue?
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Can QuantiFast Probe Assays be ordered offline via e-mail or fax?
FAQ ID - 3278
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3283-Do you have any information or guidelines regarding the choice of reference genes for real-time PCR?
Visualizar
3285-How are the genes in each iPSC qBiomarker PCR Array selected?
Visualizar
Can the QuantiFast Probe Assay detect short fragmented RNA as well as longer fragments of RNA?
Visualizar
3284-For which applications can the RNA isolated with the RNeasy Plus Universal Kit also be used?
Visualizar
Do the RNeasy Plus Universal Mini/Midi kits contain both wash buffers, RW1 and RWT?
FAQ ID - 3282
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3270 - Are the primer sets different for the Investigator Nonaplex ESS Kit and ESSplex SE where the loci are the same?
Visualizar
Are the QuantiFast Probe Assays hydrolysis or FRET probes?
FAQ ID - 3271
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Are panels and bin sets available for use with GeneMapper ID and GeneMapper ID-X?
FAQ ID - 3267
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3275 - Can I use more than 10ng DNA for the PCR reaction for my therascreen Pyro Kits?
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Can I use more than 10ng DNA for the PCR reaction for the Pyro kits?
FAQ ID - 3276
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3274 - Can I do singleplex PCR with the QuantiFast Probe Assays?
Visualizar
3272 - Are the QuantiFast Probe Assays wet-lab validated?
Visualizar
3273 - At what temperature can I perform the experiment with the NexTal Cubic Phase kits?
Visualizar
3222 - How much DNA/RNA can be isolated with the AllPrep DNA/RNA FFPE Kit?
Visualizar
3218 - How many fragments are contained within the size standard BTO in the Investigator Nonaplex ESS Kit?
Visualizar
3219 - How many freeze-thaw cycles can the master mix contained in the QuantiFast Probe RT-PCR Plus Kit tolerate?
Visualizar
3220 - How many PCR cycles should I use for the Investigator Nonaplex ESS Kit?
Visualizar
3226 - How pure should the chloroform used with the RNeasy Plus Universal Mini Kit be?
Visualizar
3223 - How much sample material can I use for DNA/RNA purification with the AllPrep DNA/RNA FFPE Kit?
Visualizar
3224 - How much time can be saved when using the RNeasy Mini QIAcube Kit?
Visualizar
3289-How does the QuantiFast Probe RT-PCR Plus Kit eliminate genomic DNA contamination?
Visualizar
3290-How do I analyze the data for the particular iPSC qBiomarker Arrays I have ordered?
Visualizar
3288-How does the gDNA eliminator solution of the RNeasy Plus Universal Kit work?
Visualizar
3286-How can I adjust the ROX concentration in the QuantiFast Probe RT-PCR Plus master mix?
Visualizar
3294-How long can I store my purified DNA/RNA isolated with the AllPrep DNA/RNA FFPE kit?
Visualizar
3291-How do I harvest the crystals from meso phase? Are special tools needed with the NexTal Cubic Phase products?
Visualizar
3246 - What is the correct matrix to use with Investigator ESSplex SE Plus Kit?
Visualizar
3297-Why does the PCR for HLA-DRB4 result in 2 amplicons of different size?
Visualizar
3266 - Why are two different exon 2 forward sequencing primers used for the SBTexcellerator HLA-DRB1 Kit?
Visualizar
3215 - What is the starting material for SBTexcellerator HLA Kits?
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How can I find out if my gene of interest is expressed in a specific tissue type or cell line?
FAQ ID - 3214
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What batch sizes can be selected with the PAXgene Blood RNA MDx Kit?
FAQ ID -2491
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What are typical yields of bisulfite converted DNA when using the EpiTect Plus Bisulfite Kits?
FAQ ID -2408
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What are the labels of the probe which is used in the Internal Control Assay for detection of the IC?
FAQ ID -2449
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What is the detection limit for the QuantiFast Pathogen + IC kits?
FAQ ID -2453
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Is there a critical step during upfront sample handling when using the PAXgene Blood RNA MDX kit?
FAQ ID -2493
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What is the difference between QuantiTect Virus Kit and the new QuantiFast Pathogen +IC Kits?
FAQ ID -2448
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Which EpiTect Bisulfite Plus kit do you recommend for blood samples, cells, and tissues?
FAQ ID -2411
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Where can I find general information about RT-PCR and PCR arrays such as information about sample preparation, reverse transcription, quantitative PCR, real-time PCR, and PCR arrays?
FAQ ID -2446
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In which solution can I keep my protein for the NexTal Cubic Phase kits?
FAQ ID -2416
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What is the maximum number of batches for the QIAsymphony PAXgene Blood RNA protocol for a single run?
FAQ ID -2498
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What is the nature of the Internal Control in the QuantiFast Pathogen + IC kit?
FAQ ID -2450
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Why should I use the EpiTect Plus FFPE Bisulfite Kit instead of isolating FFPE DNA first and then doing the bisulfite conversion?
FAQ ID -2412
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Why should I work with diluted or undiluted screens?
FAQ ID -2419
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What is the difference between sponge, cubic and lamellar phase of the NeXtal CubicPhase crystallisation?
FAQ ID -2420
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Where are the somatic mutations published?
FAQ ID -2429
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What kind of control samples should I use for the particular iPSC-qBiomarker Arrays I have ordered?
FAQ ID -2441
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What should be the concentration of my protein for the Nextal Cubic Phase products?
FAQ ID -2417
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What is the PAXgene 96 filter plate?
FAQ ID -2486
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What is the range indicated by 'Std. Error' on 'Results' page?
FAQ ID -2456
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Can the Internal Controls be ordered separately in the QuantiFast Pathogen + IC kit for use during purification?
FAQ ID -2451
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Can the PAXgene Blood RNA MDx Kit be used with both the BioRobot Universal System and the BioRobot MDx?
FAQ ID -2489
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Can the PAXgene Blood RNA MDx Kit be used for diagnostic or prognostic procedures?
FAQ ID -2488
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Can the PAXgene Blood miRNA System be used for diagnostic or prognostic procedures?
FAQ ID -2494
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Can the QIAsymphony PAXgene Blood RNA System be used for diagnostic or prognostic procedures?
FAQ ID -2496
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How are the different mutations for a single gene normalized on the qBiomarker Somatic Mutation PCR array?
FAQ ID -2428
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At what temperature can I perform the NexTal Cubic Phase experiment and incubate the plate?
FAQ ID -2415
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How can I check DNA sample quality before using the Somatic Mutation PCR Arrays & Assays?
FAQ ID -2430
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How much DNA starting sample is needed for analysis with a Somatic Mutations PCR Array? And Assay?
FAQ ID -2431
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How do I get started using the NeXtal CubicPhase kits?
FAQ ID -2413
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After bisulfite conversion, can the DNA be stored?
FAQ ID -2409
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Can I buy QIAquick and MinElute columns separately?
FAQ ID -2460
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How many samples can be processed with the QIAsymphony PAXgene Blood RNA Kit?
FAQ ID -2497
Visualizar
How pure should my protein be with the NexTal CubicPhase kits?
FAQ ID -2414
Visualizar
How many samples can be processed with the PAXgene Blood RNA MDx Kit?
FAQ ID -2490
Visualizar
How many PAXgene Blood RNA Tubes can be processed per PAXgene 96 Blood RNA Kit well plate?
FAQ ID -2485
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In the REST software, what is the range indicated by 'Std. Error' on the 'Results' page?
FAQ ID -2455
Visualizar
If less than 48/96 samples are being processed, is it possible to leave positions empty with the PAXgene Blood RNA MDx Kit?
FAQ ID -2492
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How do I determine if my gene expression results are statistically significant in the REST software?
FAQ ID -2458
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How does the DNA Protection work in the EpitTect Bisulfite kits?
FAQ ID -2410
Visualizar
How is the hypothesis test performed in the REST software?
FAQ ID -2459
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How many different genes and gene mutations are on a Somatic Mutation PCR Array?
FAQ ID -2427
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How long does a QuantiFast Pathogen + IC run take on the Rotor-Gene Q?
FAQ ID -2452
Visualizar
How do I know in which phase of my NeXtal CubicPhase cristallisation experiment I am?
FAQ ID -2421
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How do I harvest the crystals from meso phase? Are special tools needed?
FAQ ID -2422
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How great is the enrichment of miRNA when using the dedicated PAXgene Blood miRNA Kit compared to the PAXgene Blood RNA Kit?
FAQ ID -2495
Visualizar
What are important points regarding the genomic DNA wipeout buffer in the QuantiFast Probe RT-PCR Plus Kit?
FAQ ID -2359
Visualizar
Will the sequence of the QuantiFast Probe Assays be provided?
FAQ ID -2369
Visualizar
Why are there various different QuantiFast Probe Assays for my gene (previous versions, transcript variants)?
FAQ ID -2366
Visualizar
Why are samples centrifuged at 4°C after addition of chloroform to the QIAzol lysates in following kits: RNeasy Lipid Tissue Mini/Midi kits, RNeasy Microarray Tissue Mini kit, RNeasy Plus Universal Mini/Midi kit, RNeasy 96 Universal Tissue kit, miRNeasy Mini kit, miRNeasy Micro kit, miRNeasy 96 kit and miRNeasy Serum/Plasma kit?
FAQ ID -2345
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Why are the QuantiFast Probe Assays located on a single exon, thereby also detecting genomic DNA?
FAQ ID -2365
Visualizar
What dedicated QIAcube Kits are available?
FAQ ID -2337
Visualizar
What are the recommended storage conditions of the QuantiFast Probe RT-PCR Plus Kit components?
FAQ ID -2355
Visualizar
What are the main differences between the qBiomarker PCR Arrays and the RT2 Profiler PCR Arrays?
FAQ ID -2438
Visualizar
What are the differences between the existing QuantiFast Probe RT-PCR Kit and the new Plus Kit?
FAQ ID -2358
Visualizar
What is the concentration of the primers and the probe in a reaction using the QuantiFast Probe Assays?
FAQ ID -2370
Visualizar
What is the average length of a QuantiFast Probe Assay?
FAQ ID -2374
Visualizar
Is the RNeasy Plus Universal Mini Kit suited for adipose, brain, and other fatty tissues?
FAQ ID -2343
Visualizar
Is there a size limit to the protein I can crystallize with the NexTal Cubic Phase products?
FAQ ID -2331
Visualizar
Is the T790M mutation included in the EGFR PCR kit?
FAQ ID -2314
Visualizar
Is it necessary to perform calibration steps for the use of the MAX dye in duplex RT-PCR experiments on different cyclers?
FAQ ID -2373
Visualizar
Is there also a miRNeasy Plus Universal Kit available?
FAQ ID -2346
Visualizar
I use cultured cells as starting material. Can I also use the RNeasy Plus Universal Kit?
FAQ ID -2342
Visualizar
I would like to order the QuantiFast Probe Assays without master mix. Is this possible?
FAQ ID -2360
Visualizar
My ADAPT report states "Run Invalid" and does not display any sample results. Why is this?
FAQ ID -2306
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Is the yield and quality of RNA and DNA purified with the AllPrep DNA/RNA FFPE Kit comparable to yield and quality of nucleic acids purified separately with other methods.
FAQ ID -2349
Visualizar
Is V600E the only mutation that can be detected with the codon 600 assay in the BRAF Pyro Kit?
FAQ ID -2382
Visualizar
Which Rotor-Gene Q rotor should be used with the EGFR RGQ PCR kit?
FAQ ID -2315
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Which kit, the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit, do you recommend for transfection?
FAQ ID -2320
Visualizar
Which probe labels are available for the QuantiFast Probe Assays?
FAQ ID -2361
Visualizar
Which purification kits are recommended for the Pyro kits?
FAQ ID -2380
Visualizar
What should be the concentration of my protein with the NexTal Cubic Phase products?
FAQ ID -2325
Visualizar
What is the maximum plasmid size which I can use with the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit?
FAQ ID -2316
Visualizar
What plug-ins are available for the Pyrosequencing kits?
FAQ ID -2381
Visualizar
Which crystallization solutions are compatible with lipidic cubic phase crystallization?
FAQ ID -2418
Visualizar
Which deparaffinization method can I use with the AllPrep DNA/RNA FFPE Kit?
FAQ ID -2352
Visualizar
What species are QuantiTect Probe Assays offered for?
FAQ ID -2367
Visualizar
Is it also possible to isolate miRNA with the AllPrep DNA/RNA FFPE Kit?
FAQ ID -2348
Visualizar
Can the insertions in Exon 20 be detected with the EGFR Pyro Kit?
FAQ ID -2383
Visualizar
Can the QuantiFast Probe Assay detect short fragmented RNA as well as longer fragments of RNA?
FAQ ID -2364
Visualizar
Can the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit be used for low-copy number plasmids?
FAQ ID -2317
Visualizar
Can I use the RNeasy Plus Universal Mini Kit for all types of tissue?
FAQ ID -2338
Visualizar
Can negative crossing threshold (CT) values be negative when using the KRAS and EGFR PCR kits?
FAQ ID -2300
Visualizar
Can QuantiFast Probe Assays be ordered offline via e-mail or fax?
FAQ ID -2368
Visualizar
For the the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit, can plates be partially used?
FAQ ID -2319
Visualizar
For which applications can the RNA isolated with the RNeasy Plus Universal Kit also be used?
FAQ ID -2339
Visualizar
Does the KRAS PCR kit cover the codon 61 mutation?
FAQ ID -2308
Visualizar
Do you have any information or guidelines regarding the choice of reference genes for real-time PCR?
FAQ ID -2371
Visualizar
Are the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit suited for short hairpin vectors?
FAQ ID -2318
Visualizar
Are the QuantiFast Probe Assays hydrolysis or FRET probes?
FAQ ID -2363
Visualizar
Are the buffers in the RNeasy QIAcube kit delivered in QIAcube buffer bottles?
FAQ ID -2334
Visualizar
Are QIAshredder spin columns delivered with the RNeasy Mini QIAcube Kit?
FAQ ID -2333
Visualizar
Can I use more than 10ng DNA for the PCR reaction for the Pyro kits?
FAQ ID -2379
Visualizar
Can I do singleplex PCR with the QuantiFast Probe Assays?
FAQ ID -2362
Visualizar
Are the QuantiFast Probe Assays wet-lab validated?
FAQ ID -2375
Visualizar
Are you able to supply a Certificate of Analysis for the KRAS and EGFR PCR kits?
FAQ ID -2301
Visualizar
At what temperature can I perform the experiment with the NexTal Cubic Phase kits?
FAQ ID -2323
Visualizar
How much can the mixed standard ?Ct values vary in the KRAS PCR kit?
FAQ ID -2307
Visualizar
How many samples can be processed per RNeasy Mini QIAcube kit?
FAQ ID -2332
Visualizar
How much DNA/RNA can be isolated with the AllPrep DNA/RNA FFPE Kit?
FAQ ID -2353
Visualizar
How many freeze-thaw cycles can the master mix contained in the QuantiFast Probe RT-PCR Plus Kit tolerate?
FAQ ID -2357
Visualizar
How pure should the chloroform used with the RNeasy Plus Universal Mini Kit be?
FAQ ID -2344
Visualizar
How much sample material can I use for DNA/RNA purification with the AllPrep DNA/RNA FFPE Kit?
FAQ ID -2351
Visualizar
How much time can be saved when using the RNeasy Mini QIAcube Kit?
FAQ ID -2335
Visualizar
How does the gDNA eliminator solution of the RNeasy Plus Universal Kit work?
FAQ ID -2341
Visualizar
How does the QuantiFast Probe RT-PCR Plus Kit eliminate genomic DNA contamination?
FAQ ID -2354
Visualizar
How do I analyze the data for the particular iPSC qBiomarker Arrays I have ordered?
FAQ ID -2440
Visualizar
How are the genes in each iPSC qBiomarker PCR Array selected?
FAQ ID -2439
Visualizar
How can I adjust the ROX concentration in the QuantiFast Probe RT-PCR Plus master mix?
FAQ ID -2356
Visualizar
How long can I store my purified DNA/RNA isolated with the AllPrep DNA/RNA FFPE kit?
FAQ ID -2350
Visualizar
How is the RNeasy Plus Universal Kit different from the RNeasy Plus Kit?
FAQ ID -2340
Visualizar
How do I harvest the crystals from meso phase? Are special tools needed with the NexTal Cubic Phase products?
FAQ ID -2330
Visualizar
Is a special processing protocol needed?
FAQ ID -2523
Visualizar
What is the detection limit for the Investigator Quantiplex Kit?
FAQ ID -2572
Visualizar
Is it possible to archive PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue blocks?
FAQ ID -2524
Visualizar
The disruption of my sample worked well with the TissueLyser LT, but I am not satisfied with the RNA yield. What do you suggest?
FAQ ID -2555
Visualizar
What are the dimensions of the TissueLyser LT?
FAQ ID -2545
Visualizar
On which cyclers can the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit be used?
FAQ ID -2596
Visualizar
What is the average RNA yield from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -2534
Visualizar
What do I have to consider if I am using Applied Biosystems SDS software version 1.2.3 to run an Investigator Quantiplex reaction?
FAQ ID -2570
Visualizar
What is in the elution buffer in the PAXgene Bone Marrow RNA kit?
FAQ ID -2511
Visualizar
What are the storage conditions for the mericon Assay kits?
FAQ ID -2540
Visualizar
Is the performance of the HiPerFect HTS Reagent affected if it has accidentally been stored at°C?
-20
Visualizar
Is it possible to embed PAXgene Tissue fixed and stabilized samples in Optimal Cutting Temperature (OCT) medium for freezing?
FAQ ID -2525
Visualizar
Is it possible to microdissect PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissues?
FAQ ID -2531
Visualizar
Is the morphology after H&E staining comparable to formalin-fixed samples?
FAQ ID -2526
Visualizar
Is RNA from red blood cells also isolated when RNA is extracted from a PAXgene Bone Marrow RNA Tube?
FAQ ID -2509
Visualizar
Is the 260/230 nm absorbance ratio specified for RNA derived from the PAXgene Bone Marrow RNA System?
FAQ ID -2514
Visualizar
Which version of the Rotor-Gene Q software can be used to run the Investigator Quantiplex?
FAQ ID -2568
Visualizar
What is the maximum tissue size that can be fixed in a PAXgene Tissue Container?
FAQ ID -2518
Visualizar
Why do I observe a CT shift for the Internal Control in some samples in comparison to the Ct value for the Internal Control of the dilution series of the Control DNA Z1?
FAQ ID -2575
Visualizar
What RNA yields are expected with the PAXgene Bone Marrow RNA System?
FAQ ID -2506
Visualizar
What is the RT-PCR performance of RNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissue compared to RNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?
FAQ ID -2538
Visualizar
What is the nature of the heterologous Internal Amplification Control and Positive Control in the mericon Assay kits?
FAQ ID -2543
Visualizar
What is the expected amplicon size when using the mericon Assay kits?
FAQ ID -2544
Visualizar
What is the longest period you recommend to leave out the dilute HiPerFect HTS at RT before use?
FAQ ID -2560
Visualizar
What is the typical amount of tissue used for disruption with the TissueLyser LT?
FAQ ID -2552
Visualizar
What kind of sample tubes are recommended to use with the TissueLyser LT?
FAQ ID -2553
Visualizar
What is the purity of nucleic acids extracted with the PAXgene Tissue kits?
FAQ ID -2533
Visualizar
Can the PAXgene Bone Marrow RNA System be used to isolate genomic DNA or viral RNA and DNA?
FAQ ID -2507
Visualizar
Can the PAXgene Bone Marrow RNA System be used with animal bone marrow or with blood samples?
FAQ ID -2501
Visualizar
Can the PAXgene RNA stabilization solution/bone marrow mixture from the PAXgene Bone Marrow RNA Tube be disposed of by adding bleach?
FAQ ID -2515
Visualizar
Can sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for other histochemical staining techniques, such as PAS?
FAQ ID -2530
Visualizar
Can the PAXgene Bone Marrow RNA System be used for other human body fluids than bone marrow samples or cultured cells of human origin?
FAQ ID -2502
Visualizar
Can the sample tubes and the TissueLyser LT Adapter be cooled with liquid nitrogen?
FAQ ID -2549
Visualizar
Can the TissueLyser LT Adapter be removed for cleaning?
FAQ ID -2546
Visualizar
Can the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit be used with hybridization probes?
FAQ ID -2595
Visualizar
Can the samples on the TissueLyser LT be protected from degradation of nucleic acids or proteins?
FAQ ID -2548
Visualizar
When using HiPerFect HTS Reagent is it necessary to optimize amount of siRNA and transfection reagent?
FAQ ID -2556
Visualizar
When using the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit, what should the fluorescent label of the probe be for the customer-defined assay to detect the pathogen?
FAQ ID -2594
Visualizar
Which human target is used for the quantification in the Investigator Quantiplex Kit?
FAQ ID -2577
Visualizar
What sample numbers can be processed on the TissueLyser LT?
FAQ ID -2547
Visualizar
What size bead should I use for tougher samples with the TissueLyser LT?
FAQ ID -2554
Visualizar
When performing the Internal Control assay for the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit, what channel or filter can be used to detect MAX?
FAQ ID -2597
Visualizar
Why is the fluorescence signal of the Internal Control on my Rotor-Gene Q very low?
FAQ ID -2574
Visualizar
Which version of the Applied Biosystems 7500 Real-Time PCR System software can be used to run the Investigator Quantiplex?
FAQ ID -2569
Visualizar
Can specific types of white blood cells be enriched from a PAXgene Bone Marrow RNA Tube prior to the RNA isolation?
FAQ ID -2508
Visualizar
Are there any accessories required for sample disruption with the TissueLyser LT?
FAQ ID -2551
Visualizar
At what temperature should I store the Investigator Quantiplex Kit?
FAQ ID -2578
Visualizar
Can bone marrow aliquots other than 2 mL be pipetted into the PAXgene Bone Marrow RNA Tube?
FAQ ID -2503
Visualizar
Are the Internal Controls used in the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit homologous to a known target?
FAQ ID -2598
Visualizar
Can I use water to elute the RNA in the PAXgene Bone Marrow RNA Kit?
FAQ ID -2512
Visualizar
Can bone marrow from leukemia patients with elevated numbers of white blood cells be processed with the PAXgene Bone Marrow RNA System?
FAQ ID -2510
Visualizar
Can PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for in situ hybridization?
FAQ ID -2529
Visualizar
How long is the fixation time?
FAQ ID -2519
Visualizar
Can HiPerFect HTS be used for miRNA?
FAQ ID -2561
Visualizar
Can HiPerFect HTS Reagent be used for transfecting shRNAs?
FAQ ID -2559
Visualizar
How long does the sample disruption with the TissueLyser LT take?
FAQ ID -2550
Visualizar
How many times can the Internal Controls used in the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit be freeze-thawed?
FAQ ID -2599
Visualizar
How long can I store the dilutions of the Control DNA Z1 standards at 2°C?
-8
Visualizar
How long can primer and probes from the mericon Assay kits be kept at°C once reconstituted?
-20
Visualizar
How should the PAXgene Bone Marrow RNA Tubes be frozen after collection and for how long?
FAQ ID -2505
Visualizar
How long does an Investigator Quantiplex run take?
FAQ ID -2566
Visualizar
How much time may the aspirated bone marrow sample be allowed to incubate before pipetting it into the PAXgene Bone Marrow RNA Tube?
FAQ ID -2504
Visualizar
I routinely heat an aliquot of the RNA eluate prior to my RT reaction. Do I still need to heat the eluate after the PAXgene Bone Marrow RNA preparation?
FAQ ID -2513
Visualizar
Do I have to calibrate new dyes on my Applied Biosystems 7500 or 7500 Fast Real-Time PCR System?
FAQ ID -2571
Visualizar
Do you have FACS or Fluoro-Microscopy data using HiPerFect HTS Reagent for delivery of siRNA?
FAQ ID -2557
Visualizar
How can I streamline the quantification workflow for the Investigator QuantiPlex Kit?
FAQ ID -2579
Visualizar
How is stability and performance of these mericon Assay kits guaranteed?
FAQ ID -2542
Visualizar
How long are the target sequences of Investigator Quantiplex?
FAQ ID -2576
Visualizar
For which real-time cyclers has the Investigator Quantiplex Kit been validated?
FAQ ID -2567
Visualizar
How are the supernatants resulting from the first two spin steps of the PAXgene Bone Marrow RNA kit purification procedure disposed?
FAQ ID -2516
Visualizar
Visualizar
What is the part number for O-rings for QIAsymphony SP and AS instruments? How often should the O-rings be replaced?
FAQ ID -3055
Visualizar
Where can I find the ordering information for QIAcube accessories such as Shaker Rack plugs, Rack labeling strips, Reagent bottle rack, 30mL Reagent bottles, Rotor Adapter holder, Rotor adapters, Sample tubes CB, Sample tubes RB and Spin column adapter rings?
FAQ ID -3054
Visualizar
Why are two reagents used in the PAXgene Tissue System?
FAQ ID -3022
Visualizar
What is the maximum size of tissue that can be fixed in a PAXgene Tissue FIX Container (50 ml)?
FAQ ID -3024
Visualizar
5. What is the maximum size of the tissue sample that can be fixed in a PAXgene Tissue Container?
FAQ ID -3023
Visualizar
What happens if a tissue sample larger than 20 x 20 x 20 mm is used for fixation with the PAXgene Tissue FIX Container (50 ml)?
FAQ ID -3025
Visualizar
Is it possible to fix over a weekend?
FAQ ID -3027
Visualizar
How long is the fixation time when using PAXgene Tissue FIX Container?
FAQ ID -3026
Visualizar
How long can a tissue specimen stay in the PAXgene Tissue STABILIZER?
FAQ ID -3028
Visualizar
What is the minimum incubation time required when using the PAXgene Tissue STABILIZER?
FAQ ID -3029
Visualizar
Is it possible to integrate the PAXgene Tissue STABILIZER into the processing?
FAQ ID -3037
Visualizar
Is it possible to archive PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3038
Visualizar
Is the morphology after H&E staining comparable to formalin-fixed samples?
FAQ ID -3039
Visualizar
Are immunohistochemistry (IHC) assays developed for formalin-fixed, paraffin-embedded (FFPE) compatible with PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3040
Visualizar
Is it possible to use formalin-fixed, paraffin-embedded (FFPE) kits and protocols for extraction of biomolecules from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3043
Visualizar
is the purity of nucleic acids isolated with the PAXgene Tissue Kits?
FAQ ID -3044
Visualizar
What is the average RNA yield from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3045
Visualizar
What is the integrity of RNA from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3046
Visualizar
What is the RT-PCR performance of RNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue compared to RNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?
FAQ ID -3050
Visualizar
How can proteins purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be analyzed?
FAQ ID -3052
Visualizar
What is the PCR performance of DNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue compared to DNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?
FAQ ID -3051
Visualizar
What is the DNA integrity from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3047
Visualizar
Does the PAXgene Tissue RNA Kit co-purify small RNAs?
FAQ ID -3048
Visualizar
How can I extract proteins from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3049
Visualizar
Can PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for in situ hybridization?
FAQ ID -3042
Visualizar
What tubes can be used with TissueLyser Adapter Set 2 x 96 (cat. no. 69984)?
FAQ ID -3086
Visualizar
What is the concentration of the Gentra RNase A that is used with the Autopure and Puregene protocols?
FAQ ID -3083
Visualizar
What are the typical yields obtained from Buccal swabs on BioSprint 96?
FAQ ID -3071
Visualizar
Where can I find a copy of the .kpf file for a BioSprint protocol?
FAQ ID -3073
Visualizar
Can the DyeEx 96 kit be used to clean sequencing reactions to be run on ABI 3730?
FAQ ID -3075
Visualizar
Can the FastLane RT reaction be incubated longer than 30 min at 42 deg C?
FAQ ID -3079
Visualizar
Does carrier RNA interfere with the QuantiTect Whole Transcriptome amplification?
FAQ ID -3080
Visualizar
What are the advantages and disadvantages of turning on the tip “re-use” feature on the QIAgility?
FAQ ID -3064
Visualizar
How precise is the QIAgility?
FAQ ID -3063
Visualizar
How long does it take to set up a 96-well plate PCR reaction on the QIAgility?
FAQ ID -3066
Visualizar
Can I use a bar code scanner or reader on the QIAgility?
FAQ ID -3067
Visualizar
Can I use a USB drive to transfer protocols to BioSprint?
FAQ ID -3072
Visualizar
Can BioSprint 96 be connected to LIMS?
FAQ ID -3070
Visualizar
How can I find the run parameters for a BioSprint protocol that I am running?
FAQ ID -3069
Visualizar
Can the FastLane Cell lysates (containing stabilized RNA, step 8. in Fastlane protocol) be stored?
FAQ ID -3077
Visualizar
Can I use specific primers with the FastLane Kits?
FAQ ID -3078
Visualizar
What is the expected level of endotoxins in plasmid DNA purified with QIAGEN Plasmid kits, QIAfilter Plasmid kits and HiSpeed Plasmid purification kits?
FAQ ID -3082
Visualizar
What is the expected level of endotoxins in plasmid DNA purified with QIAprep spin miniprep kit?
FAQ ID -3081
Visualizar
What is the concentration of the different Proteinase K solutions sold by QIAGEN?
FAQ ID -3084
Visualizar
Where can I access Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit?
FAQ ID -3085
Visualizar
Is it possible to use a standard processor — the kind used routinely for formalin-fixed samples — for dehydration and paraffin infiltration of PAXgene Tissue treated samples?
FAQ ID -3032
Visualizar
Are PAXgene Tissue Containers suitable for long term-storage at freezing temperatures?
FAQ ID -3030
Visualizar
Is it possible to manually process PAXgene Tissue treated samples without using an automated processor?
FAQ ID -3036
Visualizar
Is a special processing protocol needed?
FAQ ID -3035
Visualizar
Is it possible to process formalin-fixed and PAXgene Tissue fixed samples together in one run?
FAQ ID -3033
Visualizar
Is it necessary to clean a processor normally used for formalin-fixed tissue before using it with PAXgene Tissue fixed tissue?
FAQ ID -3034
Visualizar
Can sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for other histochemical staining techniques, such as PAS?
FAQ ID -3041
Visualizar
When should the N2 bottle used with the QIAxcel Advanced System be changed?
FAQ ID -3001
Visualizar
Are the multicopy reference assays for Type-it CNV PCR +qC Kits and the qBiomarker assays the same?
FAQ ID -3000
Visualizar
How should I reconstitute the lyophilized RNase-Free DNase Set with the RNeasy 96 kit?
FAQ ID -3003
Visualizar
How do I clean up RNA preparations containing miRNA?
FAQ ID -3002
Visualizar
What is the thermal output for a BioRobot MDx and BioRobot Universal?
FAQ ID -3056
Visualizar
What is the thermal output for a QIAsymphony SP and AS instrument?
FAQ ID -3057
Visualizar
Can I use Tungsten Carbide beads for RNA extraction protocols using QIAGEN Kits?
FAQ ID -3053
Visualizar
What is the minimum number of samples that can be extracted on a BioSprint 96?
FAQ ID -3076
Visualizar
What is the fixation method used in the PAXgene Tissue System?
FAQ ID -3019
Visualizar
What does PAXgene Tissue FIX contain?
FAQ ID -3020
Visualizar
Can I perform the IDH 1/2 RGQ PCR test with slides containing less than 40% tumor cells?
FAQ ID -3098
Visualizar
What volume of bacterial culture can be processed using the QuickLyse Miniprep Kit?
FAQ ID -1357
Visualizar
Can the MaXtract High Density Tube be used with QIAzol to isolate RNA?
FAQ ID -1304
Visualizar
I have a Human Druggable Genome siRNA Set V2.0. Can I reorder the siRNAs from this set?
FAQ ID -1366
Visualizar
What factors determine if denatured proteins accumulate underneath, or inside the gel of the MaXtract Low and High Density Tubes?
FAQ ID -1312
Visualizar
If both MaXtract Low Density and High Density is suitable for an application, which type is preferable to use?
FAQ ID -1310
Visualizar
How will service work carried out on my QIAGEN BioRobot be documented?
FAQ ID -1331
Visualizar
If I cannot order FlexiPlate siRNA online, how do I order?
FAQ ID -1371
Visualizar
What information about my gene or siRNA do I need to order FlexiPlate siRNA?
FAQ ID -1365
Visualizar
Is it possible to use MaXtract for an organic extraction with a mixture of phenol and BCP (1 bromine chlorpropane)?
-3
Visualizar
What information is provided with FlexiPlate siRNAs?
FAQ ID -1363
Visualizar
Is it possible to elute plasmid DNA obtained with the QuickLyse Miniprep Kit in nuclease-free water?
FAQ ID -1355
Visualizar
Which formats are available for FlexiPlate siRNA?
FAQ ID -1358
Visualizar
Which parts are exchanged during a Preventive Maintenance visit for my QIAGEN BioRobot?
FAQ ID -1334
Visualizar
When isolating DNA from plant cells using CTAB, should MaXtract Low or High Density be used?
FAQ ID -1308
Visualizar
Which controls can I choose for FlexiPlate siRNA?
FAQ ID -1368
Visualizar
Will the REPLI-g UltraFast Mini Kit reaction always yield 10 µg DNA?
FAQ ID -1328
Visualizar
Why is there DNA in the no-template control reaction when using the standard REPLI-g procedure, but not when using the UltraFast procedure?
FAQ ID -1327
Visualizar
Which density gel type is contained in the yellow or green MaXtract Tubes?
FAQ ID -1315
Visualizar
What is the composition of FlexiPlate siRNA buffer?
FAQ ID -1361
Visualizar
Which software updates are included in a Premium Cover Agreement for my QIAGEN Instrument?
FAQ ID -1336
Visualizar
What is the composition of elution buffer QLE in the QuickLyse Miniprep Kit?
FAQ ID -1356
Visualizar
Can I reduce the amount of starting material below 10 ng when using the REPLI-g UltraFast Mini Kit?
FAQ ID -1325
Visualizar
Can MaXtract tubes be autoclaved prior to use?
FAQ ID -1305
Visualizar
Can I reduce the reaction time to e.g., 30 minutes, when using the REPLI-g UltraFast Mini Kit?
FAQ ID -1326
Visualizar
Can I use any type of DNA extraction method to purify template DNA for amplification using the REPLI-g Mitochondrial DNA Kit?
FAQ ID -1321
Visualizar
Can the bacterial pellet in the QuickLyse Miniprep procedure be resuspended by pipetting up and down or shortening the vortexing time?
FAQ ID -1352
Visualizar
Can several extraction steps be performed in the same MaXtract tube?
FAQ ID -1302
Visualizar
Can a phenol-chloroform ratio different from 1:1 also be used with MaXtract Low and High Density?
FAQ ID -1309
Visualizar
Can DNA isolated with MaXtract tubes be used for downstream applications such as restriction digestion, labeling, blotting, PCR, and automated sequencing?
FAQ ID -1303
Visualizar
Can I amplify mitochondrial DNA directly from whole blood or cells using the REPLI-g Mitochondrial DNA Kit?
FAQ ID -1319
Visualizar
Can I order Alexa Fluor labeled controls with FlexiPlate siRNA?
FAQ ID -1369
Visualizar
Can frozen sample material pulverized in liquid nitrogen be directly added to MaXtract tubes?
FAQ ID -1301
Visualizar
Can HP Validated siRNAs be ordered on the FlexiPlate?
FAQ ID -1367
Visualizar
Can I also screen large constructs like BACs, fosmids, and cosmids using the QIAGEN PlasmidAmp Kit?
FAQ ID -1337
Visualizar
How do I resuspend my FlexiPlate siRNAs?
FAQ ID -1360
Visualizar
How much REPLI-g amplified mitochondrial DNA should be used in a subsequent PCR reaction?
FAQ ID -1320
Visualizar
Do mutations in the target mitochondrial DNA prevent primer binding, resulting in no DNA amplification with the REPLI-g Mitochondrial DNA Kit?
FAQ ID -1323
Visualizar
Do you have stability data for genomic DNA isolated with the BioSprint Kits?
FAQ ID -1318
Visualizar
Does the addition of sample and organic solution to the MaXtract Tubes require a specific pipetting sequence?
FAQ ID -1307
Visualizar
Do you have stability data for genomic DNA isolated with the Flexigene DNA Kit?
FAQ ID -1317
Visualizar
How does a Service Support Agreement provide defined budgeting for maintaining my BioRobot?
FAQ ID -1330
Visualizar
How can I upload my gene list for FlexiPlate siRNA to GeneGlobe?
FAQ ID -1364
Visualizar
How can I increase the DNA yield from low-copy plasmids using the QuickLyse Miniprep Kit?
FAQ ID -1354
Visualizar
How can I ensure optimal performance of my QIAGEN instrument?
FAQ ID -1329
Visualizar
What is the "seed sequence" of a miRNA?
FAQ ID -2262
Visualizar
What is the turn around time for the ordering of miScript Target Protector?
FAQ ID -2254
Visualizar
What is the sample throughput of Pyrosequencing systems?
FAQ ID -2215
Visualizar
What is the processing time for 96 normalizations with the SeqTarget System?
FAQ ID -2237
Visualizar
What is the optimal range of PCR fragment sizes for normalization with the SeqTarget System?
FAQ ID -2247
Visualizar
What method do you recommend to quantify DNA before running the KRAS and EGFR PCR kit?
FAQ ID -2292
Visualizar
What technologies are used by the KRAS and EGFR PCR kits?
FAQ ID -2286
Visualizar
What kind of automation can be done with the EasyXtal 15-well Tool?
FAQ ID -2220
Visualizar
What kind of reading length can I expect when using Pyrosequencing technology for sequence analysis?
FAQ ID -2216
Visualizar
What dyes are used in the KRAS and EGFR PCR kits?
FAQ ID -2288
Visualizar
What do I do if I get amplification in my no template control (NTC) with the KRAS and EGFR PCR kits?
FAQ ID -2298
Visualizar
What format are the EasyXtal 15-well tools?
FAQ ID -2219
Visualizar
What do I need to provide when ordering a miScript Target Protector?
FAQ ID -2251
Visualizar
What is the difference of the sequence of a miScript miRNA Inhibitor and miScript Target Protector?
FAQ ID -2253
Visualizar
What is a miScript Precursor Assay?
FAQ ID -2214
Visualizar
What is the difference between probability and typicality in the Rotor-Gene ScreenClust HRM Software?
FAQ ID -2203
Visualizar
What is a miScript Target Protector?
FAQ ID -2249
Visualizar
Which transfection reagent is recommended for transfection of the miScript Target Protectors?
FAQ ID -2252
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Which variants of the EasyXtal 15-well tool are available?
FAQ ID -2221
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Why are most of my samples outside of the cluster in supervised mode using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2205
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Why are some of my samples outside of the cluster using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2204
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Why does the SeqTarget Prep Protocol require a 2-step procedure?
FAQ ID -2238
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Why does the miScript Target Protector not induce the degradation process?
FAQ ID -2263
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Why do I have wavy DNA bands on my agarose gel?
FAQ ID -2260
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What will happen, if the normalized DNA for the SeqTarget System is eluted with Buffer EB instead of EB2?
FAQ ID -2242
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When negative results are generated using the KRAS and EGFR PCR kits, do I rerun them?
FAQ ID -2294
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What type of samples can I use for the KRAS PCR and EGFR kits?
FAQ ID -2293
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What voltage should be used to run my agarose gel with the GelPilot Agarose?
FAQ ID -2261
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Which mode should I use in the Rotor-Gene ScreenClust HRM Software, supervised or unsupervised?
FAQ ID -2202
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Does the atypical look of the lyophilized FlexiTube siRNA Premix have a negative impact on performance?
FAQ ID -2267
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Does the miScript Target Protector match the target sequence?
FAQ ID -2250
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Do the KRAS and EGFR PCR kits contain a passive reference dye?
FAQ ID -2295
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Do I have to order at least 4 FlexiTube siRNA Premixes?
FAQ ID -2266
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How do I store my FlexiTube siRNA Premix?
FAQ ID -2270
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How is ARMS technology superior to alternative methods such as dye terminator sequencing?
FAQ ID -2290
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Effects of low A260/A230 ratios in RNA preparations on downstream applications
FAQ ID -2248
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How can I check the success of the normalization procedure for the SeqTarget System?
FAQ ID -2245
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How do I search for all the miScript Target Protectors for one species?
FAQ ID -2255
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Can PCR fragments be normalized for the SeqTarget System after purification with QIAquick?
FAQ ID -2240
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Can PCR fragments be normalized for the SeqTarget System without a previous purification step?
FAQ ID -2246
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Can I use a 384 well plate for the KRAS and EGFR PCR kits?
FAQ ID -2296
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Are all 3 components of the SeqTarget System required or can a different Long Range PCR Kit and primers be used?
FAQ ID -2234
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Can the miScript Precursor Assay differentiate between pri- and pre-miRNA?
FAQ ID -2211
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Does ethanol from the PCR product purification influence the normalization procedure for the SeqTarget System?
FAQ ID -2244
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What are EasyXtal 15-well Tools used for?
FAQ ID -2217
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What are Principal Components analyzed in Rotor-Gene ScreenClust HRM Software?
FAQ ID -2200
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Is the SeqTarget System compatible with standard-size PCRs?
FAQ ID -2241
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What are the amplicon lengths of the KRAS and EGFR PCR kits?
FAQ ID -2289
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What are clusters analyzed in the Rotor-Gene ScreenClust HRM Software?
FAQ ID -2201
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What does QIAGEN use for a positive control in precursor detection experiments?
FAQ ID -2212
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What controls are used in the KRAS and EGFR PCR kits to ensure reliable results?
FAQ ID -2291
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What do I do if I cannot see amplification in the FAM channel in my KRAS and EGFR PCR kits?
FAQ ID -2297
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What are the corresponding QIAGEN names for former Biotage instruments?
FAQ ID -2285
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What buffer conditions give the best resolution for agarose gel electrophoresis?
FAQ ID -2256
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What can be the reason for low DNA yields after normalization with the SeqTarget System?
FAQ ID -2239
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How much buffer should be used for agarose gel electrophoresis?
FAQ ID -2257
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How much DNA should be loaded per well of an agarose gel?
FAQ ID -2259
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How should gels be cast with the GelPilot Agarose so that optimal resolution is achieved?
FAQ ID -2258
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How long does it take to generate results using the KRAS and EGFR PCR kits?
FAQ ID -2299
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How long should PCR fragments be for the SeqTarget System?
FAQ ID -2236
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How many miScript Precursor Assays do you offer?
FAQ ID -2213
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Is it possible to use lower siRNA concentrations with FlexiTube siRNA Premix?
FAQ ID -2264
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Is one able to use FlexiTube siRNA Premix for transfection of primary adherent cell types?
FAQ ID -2268
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Is one able to use FlexiTube siRNA Premix for transfection of primary suspension cell types?
FAQ ID -2269
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If I am working with a difficult-to-transfect cell type or if I obtain only weak silencing effects what can I do?
FAQ ID -2265
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Is it possible to normalize to more or less than 500 ng with the SeqTarget System if needed?
FAQ ID -2235
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Is it possible to purify and normalize long range PCRs produced with PCR kits from other vendors for the SeqTarget System?
FAQ ID -2243
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Is it possible to purify viral RNA using FastLane Cell RT-PCR Kits?
FAQ ID -1484
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Why is the RT step with the QuantiFast RT Kits much shorter compared to QuantiTect RT Kits?
FAQ ID -1451
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What applications are offered on the QIAcube?
FAQ ID -1403
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Why is the activation time for HotStarTaq Plus Polymerase in the QuantiFast SYBR Green Kits different from that for QuantiFast Probe Kits?
FAQ ID -1449
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Why is the storage time for QuantiFast PCR Kits shorter than that for QuantiTect PCR Kits?
FAQ ID -1446
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Why is the reaction volume for QuantiFast PCR Kits lower than that for QuantiTect PCR Kits?
FAQ ID -1447
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Why is the QuantiFast denaturation step different for PCR and RT-PCR runs in the two-step protocol for the ABI 7500 and other cyclers?
FAQ ID -1442
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What is the minimum number of siRNAs per order? If I order 40 siRNAs, can I get those on 2 plates?
FAQ ID -1468
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Can purification columns from other suppliers be processed on the QIAcube?
FAQ ID -1406
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Can QIAcube protocol runs be interrupted, and subsequently be continued to completion?
FAQ ID -1414
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Are run reports and/or log files available on the QIAcube?
FAQ ID -1412
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Can I use more than 4 µl FastLane lysate in subsequent real-time RT-PCR?
FAQ ID -1480
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Are FlexiPlate siRNAs available at a higher final concentration than 10 µM?
FAQ ID -1476
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Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with QuantiFast Probe PCR Kits?
FAQ ID -1452
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Can different amounts of siRNA be ordered on one FlexiPlate?
FAQ ID -1473
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Can 2 µl reaction volumes be used with QuantiFast PCR Kits?
FAQ ID -1440
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Are there any specific cleaning solutions recommended for the QIAcube?
FAQ ID -1416
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Do the QuantiTect Primer Assays work with QuantiFast SYBR Green Kits?
FAQ ID -1439
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Do special settings have to be used for QuantiFast PCR Kits on the Eppendorf Mastercycler ep realplex?
FAQ ID -1437
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Do I need to buy special kits for the QIAcube?
FAQ ID -1402
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Do I need to discard partially used QIAcube tip racks?
FAQ ID -1418
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What information is required to reorder specific siRNAs from a siRNA Set in FlexiPlate format?
FAQ ID -1463
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What are the turnaround times for FlexiPlate siRNA orders?
FAQ ID -1478
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What are the dimensions and weight of the QIAcube?
FAQ ID -1407
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How much time will be saved when switching from standard cycling to fast cycling with QuantiFast Kits?
FAQ ID -1438
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How many reactions can I perform with the new QuantiFast Kits for real-time PCR?
FAQ ID -1425
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If FlexiPlate siRNA was accidentally redissolved in siRNA Suspension Buffer instead of water, resulting in a 2x buffer, would it affect performance of the siRNAs?
FAQ ID -1477
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How do you achieve fast cycling, yet still deliver the same performance in PCR as that achieved with standard cycling?
FAQ ID -1430
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Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube?
FAQ ID -1415
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Why is a 2-step (and not a 3-step) cycling protocol recommended for QuantiFast SYBR Green PCR Kits?
FAQ ID -1450
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