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Should cells be washed before collection?
FAQ ID - 3544
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Are there special requirements for flow sorting?
FAQ ID - 3546
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Does the REPLI-g Single Cell RNA Library Kit include adapters?
FAQ ID - 3550
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Does the REPLI-g Single Cell RNA Library Kit include a size selection step?
FAQ ID - 3551
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Is the REPLI-g Single Cell RNA Library Kit compatible with adapters and amplification primers from other suppliers?
FAQ ID - 3552
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Do I have to amplify the libraries generated with REPLI-g Single Cell RNA Library Kit to obtain full-length adapter sequences?
FAQ ID - 3553
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What is the enzyme used in the whole transcriptome amplification step?
FAQ ID - 3554
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Which cell collection methods are compatible with REPLI-g Single Cell RNA Library Kit?
FAQ ID - 3555
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Should cells be washed before collection?
FAQ ID - 3556
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Are there special requirements for flow sorting?
FAQ ID - 3558
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What is the sample input volume for REPLI-g Single Cell RNA Library Kit?
FAQ ID - 3557
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What is the maximum/minimum amount of DNA that can be used with REPLI-g Single Cell RNA Library Kit?
FAQ ID - 3549
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What kind of starting material can be used with the REPLI-g Single Cell RNA Library Kit?
FAQ ID - 3548
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Microscopic/visual confirmation of successfully sorted cells can be used to optimize sorting conditions. Can stained cells be used?
FAQ ID - 3559
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What is the expected DNA yield from 2.5 ml of blood when using the QIAsymphony, QIAcube, and salting-out precipitation method?
FAQ ID - 3510
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What is the expected purity of DNA extracted from blood samples collected in PAXgene Blood DNA Tubes IVD?
FAQ ID - 3511
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What is the minimum number of cells that can be efficiently processed with the QIAamp DNA Mini and QIAamp Blood Mini kits and what is the expected yield?
FAQ ID - 3516
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Is the quality and size of DNA extracted with the DNeasy Blood & Tissue kit good enough to generate DNA-libraries for next generation sequencing?
FAQ ID - 3517
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Is the quality and size of DNA extracted with the QIAamp DNA Mini and QIAamp Blood Mini kit good enough to generate DNA-libraries for next generation sequencing (NGS)?
FAQ ID - 3518
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What is the minimum number of cells or minimum amount of tissue that can be efficiently processed with the RNeasy mini kit?
FAQ ID - 3519
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How much protein does one vial of Protease in the QIAamp spin kits contain? What is the concentration of the resuspended Protease?
FAQ ID - 3520
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What is the RT-PCR performance of RNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue compared to RNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?
FAQ ID - 3514
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Can I use the RNeasy Fibrous Tissue Mini Kit (cat. no. 74704) to extract RNA from fibrous plant material?
FAQ ID - 3521
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Where can I find additional information for PreAnalytiX PAXgene products?
FAQ ID - 3515
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How can samples be shipped and stored prior to extraction on the Autopure LS?
FAQ ID - 3522
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How does the liquid level detection work and what is the minimal detectable volume for the conductive tips of the BioRobot Universal?
FAQ ID - 3524
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How can samples be shipped and stored prior to extraction on the Autopure LS?
FAQ ID - 3528
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What is the pipetting throughput of the BioRobot Universal?
FAQ ID - 3527
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How do I obtain the concentration of my gDNA from the data analysis using the GeneRead DNA QuantiMIZE?
FAQ ID - 3530
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What specification do you have for the high-speed shaker system of the BioRobot Universal?
FAQ ID - 3526
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What is the pipetting throughput of the BioRobot Universal?
FAQ ID - 3525
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Buffer SB1 in the GeneRead Size Selection Kit turned yellowish from a new kit. Can I still use it?
FAQ ID - 3562
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What is the sequence of the index in the GeneRead DNA Adapter I Set 1plex (catalog number 180912)?
FAQ ID - 3561
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Is it possible to use the REPLI-g WTA Single Cell kit to amplify miRNA?
FAQ ID - 3563
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Which sample materials are CE-IVD validated for the RealStar Filovirus Screen?
FAQ ID - 3529
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What is the accuracy of the high-speed dispensing system of the BioRobot Universal?
FAQ ID - 3523
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Can I use the QuantiMIZE system for determination of gDNA quality for non-multiplex PCR enrichment methods (ex. Hybridization) (cat. No. NGQA-002, NGQC-100Y)?
FAQ ID - 3531
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What does the resuspension buffer, Buffer BG4, in the PAXgene Blood DNA kit contain?
FAQ ID - 3501
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Will the Reverse transcription control on the RT2 profiler PCR array work on any cDNA library?
FAQ ID - 3534
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I received one of the kits in the list below containing the MinElute columns, however they were left out for a while and not stored at 2-8°C upon receipt. Can I still use them? AllPrep DNA/RNA Micro, EpiTect Fast DNA Bisulfite, EpiTect Fast FFPE Bisulfite, EpiTect Fast LyseAll Bisulfite, EpiTect Plus DNA Bisulfite, EpiTect Plus FFPE Bisulfite, EpiTect Plus LyseAll Bisulfite, exoRNeasy Serum/plasma Maxi, exoRNeasy Serum/Plasma Midi, GeneRead DNA FFPE, GeneRead rRNA Depletion, GeneRead Size Selection, MinElute Gel Extraction, MinElute PCR Purification, MinElute Reaction Cleanup, miRNeasy FFPE, miRNeasy Micro, miRNeasy Serum/Plasma, QIAamp DNA FFPE, QIAamp DNA Investigator, QIAamp DNA Micro, QIAamp MinElute Media, QIAamp MinElute Virus Spin, QIAamp MinElute Virus Vacuum, RNeasy FFPE, RNeasy Micro, RNeasy Plus Micro
FAQ ID - 3560
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There is no barcode on the QIAsymphony cooling adapter, and the instrument asks for one. What do you suggest I do?
FAQ ID - 3532
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Can I add bleach to the sample preparation waste?
FAQ ID - 3505
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What downstream applications have been tested with DNA purified using the PAXgene Blood DNA System?
FAQ ID - 3506
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Can purified RNA be used with the miScript Single Cell qPCR Kit?
FAQ ID - 3566
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What is the recommended range of cells for the miScript Single Cell qPCR Kit?
FAQ ID - 3565
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Can the miScript Single Cell qPCR Kit be used to quantify piRNA expression?
FAQ ID - 3567
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Can the miScript Single Cell qPCR Kit be used with plant cells?
FAQ ID - 3568
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Are any of the miScript Single Cell qPCR Kit components interchangeable with miScript II RT Kit, miScript Plant RT Kit, miScript PreAMP PCR Kit, or miScript Microfluidics PreAMP Kit components?
FAQ ID - 3569
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Should miScript PreAMP Primer Mixes be used in conjunction with the miScript Single Cell qPCR Kit?
FAQ ID - 3570
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Is the cDNA cleanup step in the miScript Single Cell qPCR Kit workflow absolutely required?
FAQ ID - 3571
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I have accidentally pipetted beads when removing the eluate after cDNA cleanup. What should I do?
FAQ ID - 3572
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When performing real-time PCR quality control associated with the miScript Single Cell qPCR Kit, is it necessary to perform control experiments for all individual samples/cells?
FAQ ID - 3576
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Is the miRNA reverse transcription control (miRTC) used in conjunction with the miScript Single Cell qPCR Kit?
FAQ ID - 3573
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Does the miScript Single Cell qPCR Kit contain controls?
FAQ ID - 3574
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I do not see any miRNA expression for my miRNAs of interest after performing single cell miRNA expression analysis with the miScript Single Cell qPCR Kit and miScript miRNA PCR Arrays or miScript Primer Assays. What could be the cause?
FAQ ID - 3577
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What is the amplicon size of PCR products generated with the miScript Single Cell qPCR System?
FAQ ID - 3578
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What is the dissociation curve temperature of PCR products generated with the miScript Single Cell qPCR System?
FAQ ID - 3579
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Do you recommend normalizing single cell miRNA expression data to a “housekeeping” gene or miRNA?
FAQ ID - 3580
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When performing real-time PCR quality control associated with the miScript Single Cell qPCR Kit, is it necessary to perform control experiments for all individual samples/cells?
FAQ ID - 3575
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How should data be analyzed when the miScript Single Cell qPCR Kit is used with miScript miRNA PCR Arrays or miScript Primer Assays?
FAQ ID - 3581
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What single cell isolation methods are recommended for use with the miScript Single Cell qPCR Kit?
FAQ ID - 3582
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How would I provide information on the QuantiTect Primer assays in publications when the sequence is confidential?
FAQ ID - 3593
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Is clot detection possible on the QIAsymphony?
FAQ - ID 3588
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How can I determine if a tube is suitable for clot detection on QIAsymphony?
FAQ ID - 3590
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In which tubes is clot detection possible on QIAsymphony?
FAQ ID - 3589
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Which barcodes on sample tubes are accepted by the QIAsymphony SP?
FAQ ID - 3598
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How can I create a report/logfile on my QIAsymphony SP system?
FAQ ID - 3594
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Is it possible to measure the proteolytic activity of proteins on the surface of exosomes that were isolated with ExoEasy?
FAQ ID - 3599
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Can I change the language on my QIAsymphony SP system?
FAQ ID - 3595
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Can I use blood, cells or bacteria with the QIAamp Fast DNA Tissue Kit?
FAQ ID - 3587
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