Product FAQ - QIAGEN



Product FAQ Bookmark Share more.. You are not authorized to download the resource Sort options Sort alphabetically Sort by relevance Sorted by relevance Can the Rotor-Gene Q be connected to LIMS? FAQ ID - 3596 View Can the REPLI-g Mitochondrial DNA Kit be used for species other than humans? FAQ ID - 3584 View How should frozen PAXgene Blood DNA Tubes (IVD) be thawed? FAQ ID - 3586 View How should blood samples drawn into PAXgene Blood DNA Tubes (IVD) be stored? FAQ ID - 3585 View What is MOI? FAQ ID -2768 View What is RNA interference (RNAi)? FAQ ID -2755 View What is the best approach for determining where to set the CT threshold when you have >15 samples? Is it best to go through all of them, looking for a range of best fit, and then just choose one value that fits all of them? FAQ ID -2705 View What file format and layout do I need to upload my data into the PCR Array Data Analysis software? FAQ ID -2700 View What are the key factors for success in an RNAi experiment? FAQ ID -2758 View What does the RT² qPCR Primer Assay product information mean when it says that it recognizes another transcript of the same gene? FAQ ID -2712 View What do I need in order to set up an RNAi experiment? FAQ ID -2757 View What do I need to complete a RT² qPCR Primer Assay? FAQ ID -2707 View What are the SureSilencing shRNA Plasmids? FAQ ID -2784 View What criteria should one use in choosing between siRNA versus shRNA for their studies? FAQ ID -2771 View What does a typical EpiTect Methyl qPCR Assay or Array include? FAQ ID -2740 View Why did the real-time PCR yield Ct values < 12? FAQ ID -2727 View Why can't I find the DNA methylation qPCR Assay for my gene of interest? FAQ ID -2743 View What is the composition of Buffer RPE? FAQ ID -2797 View Why do I need to identify my real-time instrument model when placing my order for RT² qPCR Primer Assays? FAQ ID -2713 View What would happen if I used home-made PCR master mixes or master mixes from other manufacturers with the RT² products? FAQ ID -2715 View Which qPCR instrument should I use with your RT² qPCR Primer Assays? FAQ ID -2714 View Why are the RT² qPCR Primer Assays not designed to cross exon-intron junctions or boundaries? FAQ ID -2710 View Why should I use RT² SYBR Green Mastermix with RT² qPCR Primer Assays? FAQ ID -2706 View Will pipetting error affect the EpiTect Methyl qPCR Array results? FAQ ID -2741 View Will pipetting error affect the miRNA qPCR Assay results? FAQ ID -2728 View Why had my RT² SYBR Green Mastermix been working well in the past, but now does not seem to be? FAQ ID -2717 View What is the composition of Buffer S3? FAQ ID -2788 View What MOI should I use for my cells? FAQ ID -2770 View What RT² qPCR Primer Assays are available? FAQ ID -2708 View What transfection reagents are compatible with the Cignal Reporter Assays? FAQ ID -2764 View What is the most reliable transfection reagent for delivering shRNA plasmids and siRNA to cells in culture? FAQ ID -2777 View What is included in the SureSilencing shRNA Plasmids shipment? FAQ ID -2785 View What is the composition of Buffer RLC? FAQ ID -2795 View What is the composition of Buffer RLT? FAQ ID -2793 View What is the composition of Buffer RLT plus? FAQ ID -2794 View What is the composition of Buffer BB? FAQ ID -2790 View What is the composition of Buffer ETR? FAQ ID -2789 View What is the composition of Buffer PB? FAQ ID -2791 View What are the key parameters contributing to unwanted off-target effects in an RNAi experiment? FAQ ID -2775 View What are the structures of the siRNA molecules used in RNAi studies? FAQ ID -2760 View What are the critical factors in designing the siRNA molecules to be used for RNAi studies? FAQ ID -2759 View Why is my EpiTect Methyl qPCR Assay "failed" as indicated in the QC page of the data analysis Excel file? FAQ ID -2734 View Why is the qRT-PCR reproducibility so critical when detecting gene expression knock down in an RNAi experiment? FAQ ID -2774 View What is the composition of gDNA Eliminator Solution? FAQ ID -2799 View What is the composition of Buffer RW1? FAQ ID -2796 View What is the minimum amount of genomic DNA required for analysis using EpiTect Methyl qPCR Arrays? FAQ ID -2739 View What is the composition of Buffer RWT? FAQ ID -2798 View Can miScript miRNA qPCR Assays be used for pre-miRNA detection? FAQ ID -2724 View Can miScript miRNA qPCR Assays detect single nucleotide difference between miRNAs? FAQ ID -2723 View Do I need an internal methylated DNA reference in the EpiTect Methyl qPCR Array? FAQ ID -2736 View Do I need the sequence specific miRNA primers in the RT step of the miRNA qPCR Assays? FAQ ID -2725 View Do you always run samples in triplicates? FAQ ID -2703 View Can you use stained (H & E) sections with RT² PreAMP cDNA Synthesis Kit? FAQ ID -2722 View Does QIAGEN guarantee the performance of the SureSilencing shRNA Plasmids? FAQ ID -2787 View Does this method with the EpiTect Methyl qPCR Array detect methylation at specific CpG dinucleotides? FAQ ID -2737 View Can I make stable cell lines with the Cignal Reporter Assays? FAQ ID -2763 View Is it acceptable to correct for transfection efficiency, when determining the level of gene expression knock down by an shRNA expression vector? FAQ ID -2783 View How are the primers in the EpiTect Methyl qPCR Array designed? FAQ ID -2735 View On which instrumentation will the RT² Profiler PCR Array work? FAQ ID -2719 View Should I use monoclonal or polyclonal antibodies in EpiTect ChIP assays? FAQ ID -2747 View What are the advantages of using the SureSilencing shRNA Plasmids over siRNA? FAQ ID -2786 View The EpiTect ChIP qPCR primers I used show very high Ct. Are there any solutions? FAQ ID -2750 View The pathway reporter luciferase activity values are greater than the positive control, is there a problem? FAQ ID -2766 View The pathway reporter luciferase activity values are less than the negative control, is there a problem? FAQ ID -2767 View How can I prevent the evaporation of reaction volume from the wells EpiTect Methyl qPCR Arrays? FAQ ID -2742 View How can I prevent the evaporation of reaction volume from the wells in the miScript miRNA PCR array? FAQ ID -2729 View How can RNA interference be used as a life science research tool? FAQ ID -2756 View How can I be sure that the restriction enzyme digestion is complete in the EpiTect Methyl qPCR system? FAQ ID -2731 View How can I identify the binding sites of my transcription factor using ChIP? FAQ ID -2753 View How can I normalize my EpiTect ChIP results? FAQ ID -2751 View What is the recommended amount of input template for each RT² qPCR Primer Assay? FAQ ID -2716 View What is the RT² Profiler PCR Array? FAQ ID -2718 View Can the EpiTect Methyl qPCR System technology reliably characterize heterogenous tissue samples? FAQ ID -2732 View Can users insert custom transcriptional regulatory elements or promoters into the Cignal Reporter Assays? FAQ ID -2765 View Can you custom design the Epitect qPCR primers for the CpG island outside of the defined promoter region? FAQ ID -2744 View Can I manually set the threshold line? FAQ ID -2702 View Can I use your EpiTect One-Day kit with transcription factors? FAQ ID -2752 View Are EpiTect Methyl qPCR Arrays or Assays applicable to FFPE samples? FAQ ID -2733 View Can I obtain the sequence of the RT² qPCR Primer Assay that I purchased? FAQ ID -2709 View Are there any unique elements that need to be incorporated into an shRNA expression vector? FAQ ID -2772 View Can I use total RNA for the miRNA PCR Arrays or Assays? FAQ ID -2726 View At what point can I stop and freeze my samples when using ChIP? FAQ ID -2754 View Can I use the default PCR program to run EpiTect Methyl qPCR Arrays or Assays? FAQ ID -2745 View Can I use your EpiTect ChIP system in a RNA study? FAQ ID -2748 View Can I transform the Cignal Reporter Assays? FAQ ID -2762 View Can I reliably detect intermediately methylated DNA with EpiTect Methyl qPCR Array technology? FAQ ID -2738 View How many experiments can I do with the Cignal Lenti Reporter Assays? FAQ ID -2769 View How many housekeeping genes are included in each PCR Array? FAQ ID -2704 View Can I use fluorescence microscopy to assess shRNA plasmid transfection efficiency in my model cell line of interest, when using a vector expressing a GFP reporter gene? FAQ ID -2782 View What are the advantages of EpiTect ChIP qPCR Assays and Arrays? FAQ ID -2721 View How reliable are the results from the miScript Primer Assays? FAQ ID -2730 View What are EpiTect ChIP qPCR Assays? FAQ ID -2720 View How do you determine the efficiency using the PCR array? FAQ ID -2701 View How do I choose EpiTect ChIP-grade antibodies? FAQ ID -2746 View How do you design your EpiTect ChIP qPCR Assay primers? Do they work with SB GR? FAQ ID -2749 View How does QIAGEN control the quality of the RT² qPCR Primer Assays? FAQ ID -2711 View How does stable transfection of a shRNA-encoding plasmid work? FAQ ID -2779 View How do I choose between the DNA-based Cignal Reporter Assays and the Cignal Lenti Reporter Assays? FAQ ID -2761 View What are the storage conditions for the Microbial DNA qPCR products? FAQ ID — 3394 View Which Microbial qPCR Mastermix should I use? FAQ ID — 3395 View What is the expected amplicon size of the Microbial DNA qPCR Assays? FAQ ID — 3396 View 3326 - Which QIAcube standard protocol might be suitable to extract RNA from saliva or from a buccal cell pellet? View 3327 - How often should the o-ring for the pipettor tip-adapter be changed on the QIAcube? View 3328 - Can the Sample Tubes CB and the Sample Tubes RB be used interchangeably? View 3329 - When using REPLI-g, if the starting material is a single sorted cell, could mitochondrial DNA (mtDNA) be amplified? View 3330 - Is there any contamination from E. coli genomic DNA in the polymerase provided with the Repli-G Single Cell Kit? View 3331 - Should the REPLI-g amplified mtDNA be cleaned up before sequencing? View 3332 - Is there a method that can distinguish between non-amplified DNA and REPLI-g amplified DNA? View 3333 - When using REPLI-g, what is the purpose of the DTT added to the denaturation buffer in the cells/blood protocol? View What sample types can be tested on the arrays/assays? FAQ ID — 3399 View What is the difference between Positive PCR Control (PPC) and Microbial DNA Positive Control? FAQ ID — 3397 View Can I use the Microbial DNA-Free Water and Microbial qPCR Mastermix if they have been opened more than 3 times? FAQ ID — 3398 View What are the minimum sample requirements for Microbial DNA qPCR kits? FAQ ID — 3393 View View View What is the QIAxcel QX RNA Size Marker 200-6000nt? FAQ ID - 3365 View What is the nature of the gel in the QIAxcel RNA QC kit v2.0? Is it denaturing? FAQ ID - 3366 View View 3352 - What are the size ranges of Ni-NTA particles? FAQ ID - 3352 View 3353 - What is the composition of the elution buffer in the Ni-NTA Fast Start Kit? FAQ ID - 3353 View 3351 - What is the upper limit for protein size that can bind to Ni-NTA agarose resin? FAQ ID - 3351 View 3354 - What type and amount of resin is packed into the Ni-NTA Spin columns from the Ni-NTA Spin Kit? FAQ ID - 3354 View 3341 - How are the EpiTect DNA controls produced and tested? FAQ - 3341 View 3344 - What is the difference between the EpiTect Plus DNA Bisulfite kit compared to the classic kit? FAQ - 3344 View 3345 - What is the difference between the high and low concentration EpiTect Bisulfite Conversion protocol? FAQ - 3345 View Why does AllPrep DNA/RNA/Protein Mini kit use buffer RLT, but Allprep DNA/RNA Mini and Allprep DNA/RNA Micro kits use buffer RLT Plus? Are these buffers interchangeable? FAQ ID - 3391 View 3311 - Can QuickLyse miniprep kits be used with QIAVac 24 Plus? View 3312 - For DirectPrep 96 miniprep, is it possible to use a half plate and save the other half for later prep? View 3313 - What is the endotoxin level for the QIAGEN Plasmid Plus kits? View 3314-What is the concentration of RNase A sold separately? View CAn I use milk or other liquids with the DNeasy mericon Food kit? What volume would I use? FAQ ID - 3350 View What is the expected purity of DNA extracted from blood samples collected in PAXgene Blood DNA Tubes (IVD)? FAQ ID - 3383 View 3316-What is the concentration of the reconstituted QuantiFast Probe Assay? View Does salt concentration in the food matter when using the DNeasy mericon Food Kit? FAQ ID - 3346 View Why does the DNeasy mericon Food Kit use a QIAquick column and Buffer PB rather than a DNeasy column and Buffer AL, which might be expected since the kit isolates genomic DNA? FAQ ID - 3347 View When would I use the mericon DNA Bacteria Kit vs the mericon DNA Bacteria Plus Kit? FAQ ID - 3348 View Why is my 260/280 ratio low after using the DNeasy mericon Food Kit? FAQ ID - 3349 View Is it possible to stop the DNeasy tissue protocol and store the tissue lysates after digesting in buffer ATL and Proteinase K? FAQ ID - 3362 View What is the composition of QIAzol? What is the color of this reagent? FAQ ID - 3355 View Are QIAcube reagent bottles (cat. no. 990393) autoclavable? What type of plastic are they made of? FAQ ID - 3369 View Show more results Back to top Contact QIAGEN Global contacts Technical Service Customer Care