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Why have the hazard symbols accompanying products changed?
FAQ ID - 3430
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Why is 'NO COM' displayed on the QIAxcel Instrument Panel although the power is on and all connections are made to the QIAxcel instrument and computer?
FAQ ID -1848
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Why is 18S ribosomal RNA (rRNA) used as a housekeeping gene to normalize sample-to-sample, systematic variation in qPCR assays?
FAQ ID -2675
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Why is a 2-step (and not a 3-step) cycling protocol recommended for QuantiFast SYBR Green PCR Kits?
FAQ ID -1450
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Why is a 2-step (and not a 3-step) cycling protocol recommended for Rotor-Gene SYBR Green Kits?
FAQ ID -2122
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Why is a blood collection set required to be used with the PAXgene Blood RNA tube?
FAQ ID - 3459
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Why is a blood collection set required to collect blood into the PAXgene Blood ccfDNA Tube?
FAQ ID - 3628
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Why is an ice-incubation step included during reaction set-up when following the QuantiTect RT-PCR but not the QuantiTect PCR protocol.
FAQ ID -283
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Why is carrier RNA used during the isolation of gDNA from microdissected samples with the QIAamp DNA Micro Kit?
FAQ ID -473
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Why is CoralLoad included in the Type-it Mutation Detect, but not in the Type-it Microsatellite PCR Kit?
FAQ ID -2068
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Why is DTT used only for semen samples?
FAQ ID -
143761
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Why is EvaGreen instead of SYBR Green used as fluorescent dye in the Type-it HRM PCR Kit?
FAQ ID -2196
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Why is it not recommended to stabilize cells with RNAprotect?
FAQ ID -941
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Why is it recommended to add 1 mM IPTG for optimal protein yields using the EasyXpress Protein Synthesis Kits?
FAQ ID -860
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Why is maximum amplicon size for the Type-it Microsatellite PCR Kit limited to 500 bp?
FAQ ID -2060
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Why is my 260/280 ratio low after using the DNeasy mericon Food Kit?
FAQ ID - 3349
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Why is my EpiTect Methyl qPCR Assay "failed" as indicated in the QC page of the data analysis Excel file?
FAQ ID -2734
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Why is my no template control (NTC) real-time Ct value < 35 cycles in my qPCR Assay?
FAQ ID -2686
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Why is my plasmid DNA yield low?
FAQ ID -768
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Why is special Type-it Fast SNP Probe PCR chemistry required for TaqMan® SNP Genotyping?
FAQ ID -2057
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Why is the activation time for HotStarTaq Plus Polymerase in the QuantiFast SYBR Green Kits different from that for QuantiFast Probe Kits?
FAQ ID -1449
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Why is the alignment marker for the 1.8 kb fragment not visible when using the 25 bp/1.8 kb DNA Size Marker together with the 15 bp/400 bp marker on the QIAxcel System and QIAxcel Advanced?
FAQ ID -1835
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Why is the copy number call lower than expected?
FAQ ID — 3413
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Why is the final heat denaturation step so important with the QIAsymphony PAXgene Blood RNA protocol?
FAQ ID -2987
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Why is the fluorescence signal of the Internal Control on my Rotor-Gene Q very low?
FAQ ID -2574
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Why is the initial activation step different for Rotor-Gene Probe, SYBR Green and Multiplex Kits?
FAQ ID -2118
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Why is the pQE DNA provided in QIAexpress Kits blue in color?
FAQ ID -487
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Why is the QIAamp Viral RNA Mini Kit (50) currently unavailable?
FAQ ID -147395
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Why is the QIAcube Connect App reacting so slowly?
FAQ ID - 141519
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Why is the qRT-PCR reproducibility so critical when detecting gene expression knock down in an RNAi experiment?
FAQ ID -2774
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Why is the QuantiFast denaturation step different for PCR and RT-PCR runs in the two-step protocol for the ABI 7500 and other cyclers?
FAQ ID -1442
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Why is the reaction volume for QuantiFast PCR Kits lower than that for QuantiTect PCR Kits?
FAQ ID -1447
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Why is the RT step with the QuantiFast RT Kits much shorter compared to QuantiTect RT Kits?
FAQ ID -1451
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Why is the storage time for QuantiFast PCR Kits shorter than that for QuantiTect PCR Kits?
FAQ ID -1446
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Why is the Type-it Mutation Detect PCR Kit recommended for preamplification of SNPs?
FAQ ID -2067
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Why is there an asterisk for my miScript Primer Assays?
FAQ ID -2176
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Why is there DNA in the no-template (negative) control when using the QuantiTect Whole Transcriptome Kit?
FAQ ID -1620
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Why is there DNA in the no-template control reaction when using the standard REPLI-g procedure, but not when using the UltraFast procedure?
FAQ ID -1327
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Why is there more than one QIAgene for my gene of interest?
FAQ ID -2044
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Why is there no detection of free reporter fluorescence in the LiquiChip System?
FAQ ID -732
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Why is there no male DNA obtained from sexual assault samples?
FAQ ID -
143767
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Why is there no QuantiTect Primer Assay for my gene of interest?
FAQ ID -1140
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Why is there no vacuum-only protocol for the RNeasy Plus 96 Kit?
FAQ ID -1993
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Why is there RNA in the DNA eluate when using the AllPrep DNA Mini Spin Column?
FAQ ID -1044
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Why might Affymetrix GeneChip Mapping assays interfere with the REPLI-g FFPE kit?
FAQ ID -1755
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Why might my gene of interest drop out during WGA if it is not near a telomere or centromere?
FAQ ID -703
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Why should DNA or cDNA targets be less than 250 bp long for real-time PCR?
FAQ ID-751
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Why should I use RT² SYBR Green Mastermix with RT² qPCR Primer Assays?
FAQ ID -2706
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Why should I use the EpiTect Plus FFPE Bisulfite Kit instead of isolating FFPE DNA first and then doing the bisulfite conversion?
FAQ ID -2412
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Why should I use the RNeasy Microarray Tissue Mini Kit when purifying RNA for microarray analysis?
FAQ ID -2188
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Why should I work with diluted or undiluted screens?
FAQ ID -2419
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Why would clumps occur following the addition of Buffer P2 when using LyseBlue Reagent in a plasmid preparation?
FAQ ID -862
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Will acetone precipitation recommended in the Qproteome Protocols denature Protein?
FAQ ID -807
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Will biotin detection assays be efficient when doing in vitro translation from E. coli lysates with the EasyXpress Site-Specific Biotin Kit?
FAQ ID -878
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Will DNA from chromosomes collected in TE with a drop of mineral oil work with REPLI-g amplification?
FAQ ID -664
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Will dUTP in a PCR reaction affect pyrosequencing?
FAQ ID -2843
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Will integral, peripheral as well as lipid-anchored plasma membrane proteins be isolated with the Qproteome Plasma Membrane Protein Kit?
FAQ ID -1199
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Will mitochondrial DNA also be isolated using the DNA tissue protocols and QIAsymphony DSP DNA Mini Kit on the QIAsymphony SP?
FAQ ID -2991
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Will mitochondrial DNA also be isolated using the DNA Tissue protocols on the QIAsymphony SP?
FAQ ID -1921
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Will pancreas tissue swim on top of RNAprotect?
FAQ ID -536
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Will pipetting error affect the EpiTect Methyl qPCR Array results?
FAQ ID -2741
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Will pipetting error affect the miRNA qPCR Assay results?
FAQ ID -2728
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Will proteins eluted in the Qproteome Plasma Membrane Protein protocol be modified, e.g. with the ligand?
FAQ ID -1195
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Will qBiomarker Copy Number Assays work with heterogeneous samples like mixtures of tumor and normal tissue?
FAQ ID — 3419
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Will QIAseq FastSelect work in other species?
FAQ ID -147911
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Will QuantiTect Primer Assays work at an annealing temperature of 60ºC with QuantiFast SYBR Green PCR and RT-PCR Kits?
FAQ ID -1553
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Will QuantiTect Primer Assays work with Rotor-Gene SYBR Green Kits using an annealing step at 60ºC?
FAQ ID -2124
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Will REPLI-g work at high temperatures?
FAQ ID -656
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Will Rotor-Gene Kits also work on the Rotor-Gene 6000 and 3000 cyclers?
FAQ ID -2121
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Will site-specific biotin labeling with the EasyXpress Site-Specific Biotin kit alter the structure and function of my protein?
FAQ ID -877
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Will TA cloning work with REPLI-g WGA product?
FAQ ID -672
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Will the "classic" RNeasy Mini Kit be discontinued after the launch of the Allprep DNA/RNA Mini Kit?
FAQ ID -1054
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Will the 2x QIAGEN Multiplex PCR Master Mix freeze at -20°C?
FAQ ID - 525
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Will the FastLane Cell cDNA Kit work with suspension cells?
FAQ ID -801
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Will the ligand in the Qproteome Plasma Membrane Protein protocol be specific for plasma membrane exclusively?
FAQ ID -1198
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Will the optimized DNA sequence for QIAgenes be provided?
FAQ ID -2045
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Will the PhosphoSerine antibody recognize Serine residues if the neighboring residues are Leucine or Isoleucine?
FAQ ID -644
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Will the primer sequence for the PyroMark CpG Assay be provided?
FAQ ID -2823
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Will the QIAquick PCR Purification Kit remove sufficient SYBR Green from real-time PCR reactions to allow sequencing?
FAQ ID -637
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Will the QIAxcel System run using a green Data Review Software Key?
FAQ ID -1853
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Will the Qproteome Plasma Membrane Protein Kit work with starting materials other than adherent mammalian cells?
FAQ ID -1202
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Will the random hexamers in the REPLI-g reaction interfere with downstream analysis?
FAQ ID -713
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Will the REPLI-g UltraFast Mini Kit reaction always yield 10 µg DNA?
FAQ ID -1328
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Will the Reverse transcription control on the RT2 profiler PCR array work on any cDNA library?
FAQ ID - 3534
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Will the sequence of the QuantiFast Probe Assays be provided?
FAQ ID -2369
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Will the sequences of the QuantiTect Primer Assays be provided?
FAQ ID -804
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Will Uracil-N-Glycosylase (UNG) completely remove contaminating amplicons when using QuantiTect +UNG Kits?
FAQ ID -2139
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With LyseBlue reagent for lysis control, can I now process more bacterial culture and overload the columns?
FAQ ID -864
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Would co-transfection with the plasmid be expected to increase/decrease the transfection efficiency significantly?
FAQ ID -3136
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