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Is RNA co-purified using the QIAsymphony mericon Bacteria Kit?
FAQ ID -2960
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How much DNA does a bacterial cell contain?
FAQ ID -2947
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Is there a risk of crosstalk from the FAM channel to the VIC channel if the residual DNA target is present in very high copy numbers?
FAQ ID -2980
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How much DNA does a human cell contain?
FAQ ID -2948
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How much RNA does a bacterial cell contain?
FAQ ID -2949
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How many reactions can be performed with one QIAsymphony mericon Bacteria Kit?
FAQ ID -2955
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How many samples can be processed with the QIAsymphony DSP Virus/Pathogen Kits?
FAQ ID -2935
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How should the samples be stored before purification of RNA with the QIAsymphony PAXgene Blood RNA Protocol?
FAQ ID -2984
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How much DNA and RNA can be expected from human blood cells?
FAQ ID -2950
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How should the samples be stored before purification with the QIAsymphony DSP Virus/Pathogen Kits?
FAQ ID -2934
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If a cycler from Applied Biosystems is used in combination with the Certal Residual DNA Detection Kit, should dye calibration for MAX be performed?
FAQ ID -2977
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How much RNA does a typical mammalian cell contain?
FAQ ID -2946
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How much sample is required for each well for an ELISArray Kit?
FAQ ID -2908
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How much starting material can be used with the QIAsymphony DSP DNA Mini Kit on the QIAsymphony SP?
FAQ ID -2927
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What is the recommended solution in which to store RNA samples that will be used as templates for cDNA synthesis?
FAQ ID -2659
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What is the QIAxcel Advanced System?
FAQ ID -2650
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What is the purpose of the ROX and fluorescein dyes, also known as passive reference dyes, in qPCR master mixes?
FAQ ID -2671
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What is the optimal DNA concentration for the Investigator ESSplex Plus Kit?
FAQ ID -2646
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What negative controls are typically included in qPCR and/or qRT-PCR experiments?
FAQ ID -2672
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What is the SBTengine® software?
FAQ ID -2628
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What is the standard curve method for qPCR assay data analysis? How is the standard curve method for qPCR assay data analysis performed?
FAQ ID -2691
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What is the delta Rn value?
FAQ ID -2681
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What is the threshold cycle or Ct value?
FAQ ID -2682
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What is the comparative or ??Ct method for qPCR assay data analysis? How is the comparative or ??Ct method for qPCR assay data analysis performed?
FAQ ID -2693
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What is the key technical challenge in isolating high quality RNA from cell or tissue samples?
FAQ ID -2656
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What is qPCR?
FAQ ID -2668
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What is the difference between Absolute Quantification and Relative Quantification in qPCR, using the standard curve approach?
FAQ ID -2692
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Why do I see low, poor, or sub-standard amplification efficiency in my qRT-PCR assay?
FAQ ID -2695
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Is the QuantiFast SYBR Green PCR Kit compatible with the ViiA7 cycler from Applied Biosystems?
FAQ ID -2652
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What is the difference between adding the Internal Control (IC) template used in the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit to the amplification reaction versus adding the IC template at the extraction step?
FAQ ID -2600
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What is a dissociation curve, and why is it important to run a dissociation curve, following qPCR using SYBR Green chemistry?
FAQ ID -2678
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What are the most reliable methods for preparing high-quality RNA from cell or tissue samples, for use in gene expression analysis experiments?
FAQ ID -2657
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What are the differences between one-step and two-step RT--PCR?
FAQ ID -2666
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What are the guidelines for choosing a housekeeping gene for normalizing qPCR results?
FAQ ID -2674
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Why are my qPCR Ct values too high (> 35 or not detectable) in my qRT-PCR assay?
FAQ ID -2685
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Why are my qPCR Ct values too low (< 12) in my qRT-PCR Assay?
FAQ ID -2684
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Why is 18S ribosomal RNA (rRNA) used as a housekeeping gene to normalize sample-to-sample, systematic variation in qPCR assays?
FAQ ID -2675
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Why is my no template control (NTC) real-time Ct value < 35 cycles in my qPCR Assay?
FAQ ID -2686
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Why do the Internal Control templates for extraction (Internal Control DNA or RNA [High conc.]) have a 10x higher concentration than the IC templates provided with the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit?
FAQ ID -2603
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Why do I see multiple high-intensity peaks in my qPCR dissociation curve at temperatures less than 70 ºC?
FAQ ID -2690
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Why do my qPCR amplification curves or plots decrease in fluorescence intensity after the saturation phase?
FAQ ID -2689
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What should the cycler set-up be for a duplex reaction with the pathogen assay (FAM) and the Internal Control assay (MAX) on ABI instruments, when using the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit?
FAQ ID -2610
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What should the cycler set-up be for a duplex reaction with the pathogen assay (FAM) and the Internal Control assay (MAX) on Mx instruments from Stratagene (Agilent), when using the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit?
FAQ ID -2609
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What positive controls are typically included in qPCR and/or qRT-PCR experiments?
FAQ ID -2673
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What should I do if I suspect that my RNA preparation contains RNase contamination?
FAQ ID -2661
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What real-time cycler should I use for my qPCR experiments?
FAQ ID -2670
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Which DNA/RNA extraction kits were tested in combination with the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit?
FAQ ID -2606
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For the QuantiFast Pathogen RT-PCR +IC kit, does the Internal Control also control for the reverse transcription reaction?
FAQ ID -2601
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What should the Rotor-Gene Q cycler settings be for a duplex reaction with the pathogen assay (FAM) and the Internal Control assay (MAX), when using the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit?
FAQ ID -2608
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What testing should be performed to assess the quality of an RNA sample?
FAQ ID -2660
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Following thermal cycling, reaction volume has evaporated from some of the wells of my qPCR assay plate. What should I do?
FAQ ID -2683
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Do we have data on long term stability and storage of DNA purified on BioRobot M48?
FAQ ID -2611
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During data analysis, how should the threshold be set in the Yellow channel on the Rotor-Gene Q cycler to analyze the Internal Control from the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit
FAQ ID -2607
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How can I predict the percent qPCR signal due to contaminating DNA, for a given qPCR assay, and its matching NRT control?
FAQ ID -2688
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How can I ensure that reaction volume is not lost due to evaporation during thermal cycling?
FAQ ID -2679
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How can I determine whether amplification occurs from mRNA-derived cDNA or from genomic DNA contamination?
FAQ ID -2687
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How can I avoid or remove genomic DNA contamination from the total RNA preparation?
FAQ ID -2662
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Do the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit contain ROX in the master mix?
FAQ ID -2605
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Can the Internal Control DNA or RNA be added directly to the sample?
FAQ ID -2602
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Are primers available that only detect mitochondrial DNA encoded genes and not nuclear genomic DNA encoded genes?
FAQ ID -2680
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Do I need to run a standard curve before the actual PCR array experiment?
FAQ ID -2664
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Does the amount of Internal Control to be spiked into the lysis buffer or lysate depend on the elution volume only?
FAQ ID -2604
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May I try the data analysis tool without using your PCR array kit?
FAQ ID -2698
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What are the common primer and probe chemistries utilized for qPCR assays?
FAQ ID -2669
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Is the QuantiFast Multiplex PCR +R Kit compatible with the ViiA7 cycler from Applied Biosystems?
FAQ ID -2653
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Is the QuantiFast Probe PCR +ROX Vial Kit compatible with the ViiA7 cycler from Applied Biosystems?
FAQ ID -2651
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How do I determine the amplification efficiency of my qPCR assay?
FAQ ID -2694
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How do I determine the linear dynamic range of my qPCR or qRT-PCR assay?
FAQ ID -2696
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How do I create a workspace that is free of DNA contamination, prior to carrying out a qPCR experiment?
FAQ ID -2654
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How does HotStart PCR help minimize nonspecific amplification events?
FAQ ID -2676
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Is it good to pool multiple RNA replicates to detect expression changes that are consistently reproducible?
FAQ ID -2663
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How important is the RNA purification process, for obtaining reliable qRT-PCR results?
FAQ ID -2655
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3212 - What is the minimum size of DNA fragment that can be isolated with QIAamp DNA Mini kit?
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3210 - Can the 4-16K Centrifuge be used with a 208 V outlet?
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3211 - What is the maximum speed of the plate rotor 2 x 96 in the 4-16 or 4-16K?
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3202 - Can GeneRead rRNA depletion kit be used to deplete rRNA from miRNA isolated using miRNeasy kit?
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3299-Can Buffer ER from the Endofree Plasmid kits be used instead of the Buffer ETR in QIAGEN Plasmid Plus kits?
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3213 - How much ethanol should be added to Buffer AP3/E (renamed to Buffer AW1) and Buffer AW (renamed to Buffer AW2) in the DNeasy Plant kit?
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3200 - What is the Maximum/Minimum amount of RNA that can be used with GeneRead rRNA depletion kit?
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3201 - What quality of RNA can be used with the GeneRead rRNA depletion kit?
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3203 - Is the RNeasy MinElute cleanup kit required for the GeneRead rRNA depletion kit?
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3204 - How strict is the purification cut-off with GeneRead Size Selection kit?
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3205 - Can GeneRead Library preparation kits be used on species other than human mouse and rat, for example, plant species and microbial species?
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3206 - Can our GeneRead Library preparation kits be used with the SoLiD Platform?
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3248 - What is the difference between sponge, cubic and lamellar phase?
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3254 - When using the Nextal Cubic Phase products how do I know in which phase the sample is? What will I see?
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3255 - When using the NexTal Cubic Phase products, should I work with diluted or undiluted screens?
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3227 - In which solution can I keep my protein with the NexTal Cubic Phase kits?
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3217 - How long can I store my purified RNA?
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3225 - How pure should my protein be for the NexTal CubicPhase kits?
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3292-How do I start using the NeXtal CubicPhase kits?
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3295-What is the difference between SBTexcellerator HLA Core, Extended and Full Kits?
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3240 - What are the main differences between the qBiomarker PCR Arrays and the RT2 Profiler PCR Arrays?
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3237 - I would like to order the QuantiFast Probe Assays without master mix. Is this possible?
View
3233 - Is the yield and quality of RNA and DNA purified with the AllPrep DNA/RNA FFPE Kit comparable to yield and quality of nucleic acids purified separately with other methods.
View
3265 - Why are there various different QuantiFast Probe Assays for my gene (previous versions, transcript variants)?
View
3298-Will the sequence of the QuantiFast Probe Assays be provided?
View
3262 - Which thermal cyclers are validated for use with the Investigator Nonaplex ESS Kit?
View
3263 - Why are samples centrifuged at 4°C after the addition of chloroform when using the RNeasy Plus Universal Mini Kit?
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3264 - Why are the QuantiFast Probe Assays located on a single exon, thereby also detecting genomic DNA?
View
3241 - What are the recommended storage conditions of the QuantiFast Probe RT-PCR Plus Kit components?
View
3238 - What are important points regarding the genomic DNA wipeout buffer in the QuantiFast Probe RT-PCR Plus Kit?
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3239 - What are the differences between the existing QuantiFast Probe RT-PCR Kit and the new Plus Kit?
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3247 - What is the correct matrix to use with the Investigator Nonaplex ESS Kit?
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3245 - What is the concentration of the primers and the probe in a reaction using the QuantiFast Probe Assays?
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3243 - What does the Investigator Nonaplex ESS Kit contain?
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3244 -What is the average length of a QuantiFast Probe Assay?
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3231 - Is there a size limit to the protein I can crystallize with the NexTal Cubic Phase products?
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3232 - Is the RNeasy Plus Universal Mini Kit suited for adipose, brain, and other fatty tissues?
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3228 - Is it also possible to isolate miRNA with the AllPrep DNA/RNA FFPE Kit?
View
3229 - Is it necessary to perform calibration steps for the use of the MAX dye in duplex RT-PCR experiments on different cyclers?
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3236 - I use cultured cells as starting material. Can I also use the RNeasy Plus Universal Kit?
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Is there also a miRNeasy Plus Universal Kit available?
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3261 - Which purification kits are recommended for the therascreen Pyro kits?
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3258 - Which filter set and settings should be used with the Investigator Nonaplex ESS Kit?
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3260 - Which purification kits are recommended for the Pyro kits?
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3259 - Which probe labels are available for the QuantiFast Probe Assays?
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3249 - What is the optimal DNA volume and concentration for the Investigator Nonaplex ESS Kit?
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3296-Why does the A peak reduction factor have to de set to 0.86 for the codon 61 assay in the therascreen NRAS Pyro Kit?
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What plug-ins are available for the therascreen Pyro kits?
FAQ ID - 3251
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What plug-ins are available for the Pyrosequencing kits?
FAQ ID - 3250
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3252 - What should be the concentration of my protein with the NexTal Cubic Phase products?
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3256 - Which crystallization solutions are compatible with lipidic cubic phase crystallization?
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3257 - Which deparaffinization method can I use with the AllPrep DNA/RNA FFPE Kit?
View
3253 - What species are QuantiTect Probe Assays offered for?
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Can the insertions in Exon 20 be detected with the therascreen EGFR Pyro Kit?
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Can the insertions in Exon 20 be detected with the EGFR Pyro Kit?
FAQ ID - 3279
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3277 - Can I use the RNeasy Plus Universal Mini Kit for all types of tissue?
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Can QuantiFast Probe Assays be ordered offline via e-mail or fax?
FAQ ID - 3278
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3283-Do you have any information or guidelines regarding the choice of reference genes for real-time PCR?
View
3285-How are the genes in each iPSC qBiomarker PCR Array selected?
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Can the QuantiFast Probe Assay detect short fragmented RNA as well as longer fragments of RNA?
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3284-For which applications can the RNA isolated with the RNeasy Plus Universal Kit also be used?
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Do the RNeasy Plus Universal Mini/Midi kits contain both wash buffers, RW1 and RWT?
FAQ ID - 3282
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3270 - Are the primer sets different for the Investigator Nonaplex ESS Kit and ESSplex SE where the loci are the same?
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Are the QuantiFast Probe Assays hydrolysis or FRET probes?
FAQ ID - 3271
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Are panels and bin sets available for use with GeneMapper ID and GeneMapper ID-X?
FAQ ID - 3267
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3275 - Can I use more than 10ng DNA for the PCR reaction for my therascreen Pyro Kits?
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Can I use more than 10ng DNA for the PCR reaction for the Pyro kits?
FAQ ID - 3276
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3274 - Can I do singleplex PCR with the QuantiFast Probe Assays?
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3272 - Are the QuantiFast Probe Assays wet-lab validated?
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3273 - At what temperature can I perform the experiment with the NexTal Cubic Phase kits?
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3222 - How much DNA/RNA can be isolated with the AllPrep DNA/RNA FFPE Kit?
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3218 - How many fragments are contained within the size standard BTO in the Investigator Nonaplex ESS Kit?
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3219 - How many freeze-thaw cycles can the master mix contained in the QuantiFast Probe RT-PCR Plus Kit tolerate?
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3220 - How many PCR cycles should I use for the Investigator Nonaplex ESS Kit?
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3226 - How pure should the chloroform used with the RNeasy Plus Universal Mini Kit be?
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3223 - How much sample material can I use for DNA/RNA purification with the AllPrep DNA/RNA FFPE Kit?
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3224 - How much time can be saved when using the RNeasy Mini QIAcube Kit?
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3289-How does the QuantiFast Probe RT-PCR Plus Kit eliminate genomic DNA contamination?
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3290-How do I analyze the data for the particular iPSC qBiomarker Arrays I have ordered?
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3288-How does the gDNA eliminator solution of the RNeasy Plus Universal Kit work?
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3286-How can I adjust the ROX concentration in the QuantiFast Probe RT-PCR Plus master mix?
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3294-How long can I store my purified DNA/RNA isolated with the AllPrep DNA/RNA FFPE kit?
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3291-How do I harvest the crystals from meso phase? Are special tools needed with the NexTal Cubic Phase products?
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3246 - What is the correct matrix to use with Investigator ESSplex SE Plus Kit?
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3297-Why does the PCR for HLA-DRB4 result in 2 amplicons of different size?
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3266 - Why are two different exon 2 forward sequencing primers used for the SBTexcellerator HLA-DRB1 Kit?
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3215 - What is the starting material for SBTexcellerator HLA Kits?
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How can I find out if my gene of interest is expressed in a specific tissue type or cell line?
FAQ ID - 3214
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What batch sizes can be selected with the PAXgene Blood RNA MDx Kit?
FAQ ID -2491
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What are typical yields of bisulfite converted DNA when using the EpiTect Plus Bisulfite Kits?
FAQ ID -2408
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What are the labels of the probe which is used in the Internal Control Assay for detection of the IC?
FAQ ID -2449
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What is the detection limit for the QuantiFast Pathogen + IC kits?
FAQ ID -2453
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Is there a critical step during upfront sample handling when using the PAXgene Blood RNA MDX kit?
FAQ ID -2493
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What is the difference between QuantiTect Virus Kit and the new QuantiFast Pathogen +IC Kits?
FAQ ID -2448
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Which EpiTect Bisulfite Plus kit do you recommend for blood samples, cells, and tissues?
FAQ ID -2411
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Where can I find general information about RT-PCR and PCR arrays such as information about sample preparation, reverse transcription, quantitative PCR, real-time PCR, and PCR arrays?
FAQ ID -2446
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In which solution can I keep my protein for the NexTal Cubic Phase kits?
FAQ ID -2416
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What is the maximum number of batches for the QIAsymphony PAXgene Blood RNA protocol for a single run?
FAQ ID -2498
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What is the nature of the Internal Control in the QuantiFast Pathogen + IC kit?
FAQ ID -2450
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Why should I use the EpiTect Plus FFPE Bisulfite Kit instead of isolating FFPE DNA first and then doing the bisulfite conversion?
FAQ ID -2412
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Why should I work with diluted or undiluted screens?
FAQ ID -2419
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What is the difference between sponge, cubic and lamellar phase of the NeXtal CubicPhase crystallisation?
FAQ ID -2420
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Where are the somatic mutations published?
FAQ ID -2429
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What kind of control samples should I use for the particular iPSC-qBiomarker Arrays I have ordered?
FAQ ID -2441
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What should be the concentration of my protein for the Nextal Cubic Phase products?
FAQ ID -2417
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What is the PAXgene 96 filter plate?
FAQ ID -2486
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What is the range indicated by 'Std. Error' on 'Results' page?
FAQ ID -2456
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Can the Internal Controls be ordered separately in the QuantiFast Pathogen + IC kit for use during purification?
FAQ ID -2451
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Can the PAXgene Blood RNA MDx Kit be used with both the BioRobot Universal System and the BioRobot MDx?
FAQ ID -2489
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Can the PAXgene Blood RNA MDx Kit be used for diagnostic or prognostic procedures?
FAQ ID -2488
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Can the PAXgene Blood miRNA System be used for diagnostic or prognostic procedures?
FAQ ID -2494
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Can the QIAsymphony PAXgene Blood RNA System be used for diagnostic or prognostic procedures?
FAQ ID -2496
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How are the different mutations for a single gene normalized on the qBiomarker Somatic Mutation PCR array?
FAQ ID -2428
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At what temperature can I perform the NexTal Cubic Phase experiment and incubate the plate?
FAQ ID -2415
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How can I check DNA sample quality before using the Somatic Mutation PCR Arrays & Assays?
FAQ ID -2430
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How much DNA starting sample is needed for analysis with a Somatic Mutations PCR Array? And Assay?
FAQ ID -2431
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How do I get started using the NeXtal CubicPhase kits?
FAQ ID -2413
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After bisulfite conversion, can the DNA be stored?
FAQ ID -2409
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Can I buy QIAquick and MinElute columns separately?
FAQ ID -2460
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How many samples can be processed with the QIAsymphony PAXgene Blood RNA Kit?
FAQ ID -2497
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How pure should my protein be with the NexTal CubicPhase kits?
FAQ ID -2414
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How many samples can be processed with the PAXgene Blood RNA MDx Kit?
FAQ ID -2490
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How many PAXgene Blood RNA Tubes can be processed per PAXgene 96 Blood RNA Kit well plate?
FAQ ID -2485
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In the REST software, what is the range indicated by 'Std. Error' on the 'Results' page?
FAQ ID -2455
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If less than 48/96 samples are being processed, is it possible to leave positions empty with the PAXgene Blood RNA MDx Kit?
FAQ ID -2492
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How do I determine if my gene expression results are statistically significant in the REST software?
FAQ ID -2458
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How does the DNA Protection work in the EpitTect Bisulfite kits?
FAQ ID -2410
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How is the hypothesis test performed in the REST software?
FAQ ID -2459
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How many different genes and gene mutations are on a Somatic Mutation PCR Array?
FAQ ID -2427
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How long does a QuantiFast Pathogen + IC run take on the Rotor-Gene Q?
FAQ ID -2452
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How do I know in which phase of my NeXtal CubicPhase cristallisation experiment I am?
FAQ ID -2421
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How do I harvest the crystals from meso phase? Are special tools needed?
FAQ ID -2422
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How great is the enrichment of miRNA when using the dedicated PAXgene Blood miRNA Kit compared to the PAXgene Blood RNA Kit?
FAQ ID -2495
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What are important points regarding the genomic DNA wipeout buffer in the QuantiFast Probe RT-PCR Plus Kit?
FAQ ID -2359
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Will the sequence of the QuantiFast Probe Assays be provided?
FAQ ID -2369
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Why are there various different QuantiFast Probe Assays for my gene (previous versions, transcript variants)?
FAQ ID -2366
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Why are samples centrifuged at 4°C after addition of chloroform to the QIAzol lysates in following kits: RNeasy Lipid Tissue Mini/Midi kits, RNeasy Microarray Tissue Mini kit, RNeasy Plus Universal Mini/Midi kit, RNeasy 96 Universal Tissue kit, miRNeasy Mini kit, miRNeasy Micro kit, miRNeasy 96 kit and miRNeasy Serum/Plasma kit?
FAQ ID -2345
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Why are the QuantiFast Probe Assays located on a single exon, thereby also detecting genomic DNA?
FAQ ID -2365
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What dedicated QIAcube Kits are available?
FAQ ID -2337
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What are the recommended storage conditions of the QuantiFast Probe RT-PCR Plus Kit components?
FAQ ID -2355
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What are the main differences between the qBiomarker PCR Arrays and the RT2 Profiler PCR Arrays?
FAQ ID -2438
View
What are the differences between the existing QuantiFast Probe RT-PCR Kit and the new Plus Kit?
FAQ ID -2358
View
What is the concentration of the primers and the probe in a reaction using the QuantiFast Probe Assays?
FAQ ID -2370
View
What is the average length of a QuantiFast Probe Assay?
FAQ ID -2374
View
Is the RNeasy Plus Universal Mini Kit suited for adipose, brain, and other fatty tissues?
FAQ ID -2343
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Is there a size limit to the protein I can crystallize with the NexTal Cubic Phase products?
FAQ ID -2331
View
Is the T790M mutation included in the EGFR PCR kit?
FAQ ID -2314
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Is it necessary to perform calibration steps for the use of the MAX dye in duplex RT-PCR experiments on different cyclers?
FAQ ID -2373
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Is there also a miRNeasy Plus Universal Kit available?
FAQ ID -2346
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I use cultured cells as starting material. Can I also use the RNeasy Plus Universal Kit?
FAQ ID -2342
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I would like to order the QuantiFast Probe Assays without master mix. Is this possible?
FAQ ID -2360
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My ADAPT report states "Run Invalid" and does not display any sample results. Why is this?
FAQ ID -2306
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Is the yield and quality of RNA and DNA purified with the AllPrep DNA/RNA FFPE Kit comparable to yield and quality of nucleic acids purified separately with other methods.
FAQ ID -2349
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Is V600E the only mutation that can be detected with the codon 600 assay in the BRAF Pyro Kit?
FAQ ID -2382
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Which Rotor-Gene Q rotor should be used with the EGFR RGQ PCR kit?
FAQ ID -2315
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Which kit, the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit, do you recommend for transfection?
FAQ ID -2320
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Which probe labels are available for the QuantiFast Probe Assays?
FAQ ID -2361
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Which purification kits are recommended for the Pyro kits?
FAQ ID -2380
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What should be the concentration of my protein with the NexTal Cubic Phase products?
FAQ ID -2325
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What is the maximum plasmid size which I can use with the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit?
FAQ ID -2316
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What plug-ins are available for the Pyrosequencing kits?
FAQ ID -2381
View
Which crystallization solutions are compatible with lipidic cubic phase crystallization?
FAQ ID -2418
View
Which deparaffinization method can I use with the AllPrep DNA/RNA FFPE Kit?
FAQ ID -2352
View
What species are QuantiTect Probe Assays offered for?
FAQ ID -2367
View
Is it also possible to isolate miRNA with the AllPrep DNA/RNA FFPE Kit?
FAQ ID -2348
View
Can the insertions in Exon 20 be detected with the EGFR Pyro Kit?
FAQ ID -2383
View
Can the QuantiFast Probe Assay detect short fragmented RNA as well as longer fragments of RNA?
FAQ ID -2364
View
Can the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit be used for low-copy number plasmids?
FAQ ID -2317
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Can I use the RNeasy Plus Universal Mini Kit for all types of tissue?
FAQ ID -2338
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Can negative crossing threshold (CT) values be negative when using the KRAS and EGFR PCR kits?
FAQ ID -2300
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Can QuantiFast Probe Assays be ordered offline via e-mail or fax?
FAQ ID -2368
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For the the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit, can plates be partially used?
FAQ ID -2319
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For which applications can the RNA isolated with the RNeasy Plus Universal Kit also be used?
FAQ ID -2339
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Does the KRAS PCR kit cover the codon 61 mutation?
FAQ ID -2308
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Do you have any information or guidelines regarding the choice of reference genes for real-time PCR?
FAQ ID -2371
View
Are the QIAprep 96 Plus Miniprep Kit or the QIAGEN Plasmid Plus 96 Miniprep Kit suited for short hairpin vectors?
FAQ ID -2318
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Are the QuantiFast Probe Assays hydrolysis or FRET probes?
FAQ ID -2363
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Are the buffers in the RNeasy QIAcube kit delivered in QIAcube buffer bottles?
FAQ ID -2334
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Are QIAshredder spin columns delivered with the RNeasy Mini QIAcube Kit?
FAQ ID -2333
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Can I use more than 10ng DNA for the PCR reaction for the Pyro kits?
FAQ ID -2379
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Can I do singleplex PCR with the QuantiFast Probe Assays?
FAQ ID -2362
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Are the QuantiFast Probe Assays wet-lab validated?
FAQ ID -2375
View
Are you able to supply a Certificate of Analysis for the KRAS and EGFR PCR kits?
FAQ ID -2301
View
At what temperature can I perform the experiment with the NexTal Cubic Phase kits?
FAQ ID -2323
View
How much can the mixed standard ?Ct values vary in the KRAS PCR kit?
FAQ ID -2307
View
How many samples can be processed per RNeasy Mini QIAcube kit?
FAQ ID -2332
View
How much DNA/RNA can be isolated with the AllPrep DNA/RNA FFPE Kit?
FAQ ID -2353
View
How many freeze-thaw cycles can the master mix contained in the QuantiFast Probe RT-PCR Plus Kit tolerate?
FAQ ID -2357
View
How pure should the chloroform used with the RNeasy Plus Universal Mini Kit be?
FAQ ID -2344
View
How much sample material can I use for DNA/RNA purification with the AllPrep DNA/RNA FFPE Kit?
FAQ ID -2351
View
How much time can be saved when using the RNeasy Mini QIAcube Kit?
FAQ ID -2335
View
How does the gDNA eliminator solution of the RNeasy Plus Universal Kit work?
FAQ ID -2341
View
How does the QuantiFast Probe RT-PCR Plus Kit eliminate genomic DNA contamination?
FAQ ID -2354
View
How do I analyze the data for the particular iPSC qBiomarker Arrays I have ordered?
FAQ ID -2440
View
How are the genes in each iPSC qBiomarker PCR Array selected?
FAQ ID -2439
View
How can I adjust the ROX concentration in the QuantiFast Probe RT-PCR Plus master mix?
FAQ ID -2356
View
How long can I store my purified DNA/RNA isolated with the AllPrep DNA/RNA FFPE kit?
FAQ ID -2350
View
How is the RNeasy Plus Universal Kit different from the RNeasy Plus Kit?
FAQ ID -2340
View
How do I harvest the crystals from meso phase? Are special tools needed with the NexTal Cubic Phase products?
FAQ ID -2330
View
Is a special processing protocol needed?
FAQ ID -2523
View
What is the detection limit for the Investigator Quantiplex Kit?
FAQ ID -2572
View
Is it possible to archive PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue blocks?
FAQ ID -2524
View
The disruption of my sample worked well with the TissueLyser LT, but I am not satisfied with the RNA yield. What do you suggest?
FAQ ID -2555
View
What are the dimensions of the TissueLyser LT?
FAQ ID -2545
View
On which cyclers can the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit be used?
FAQ ID -2596
View
What is the average RNA yield from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -2534
View
What do I have to consider if I am using Applied Biosystems SDS software version 1.2.3 to run an Investigator Quantiplex reaction?
FAQ ID -2570
View
What is in the elution buffer in the PAXgene Bone Marrow RNA kit?
FAQ ID -2511
View
What are the storage conditions for the mericon Assay kits?
FAQ ID -2540
View
Is the performance of the HiPerFect HTS Reagent affected if it has accidentally been stored at°C?
-20
View
Is it possible to embed PAXgene Tissue fixed and stabilized samples in Optimal Cutting Temperature (OCT) medium for freezing?
FAQ ID -2525
View
Is it possible to microdissect PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissues?
FAQ ID -2531
View
Is the morphology after H&E staining comparable to formalin-fixed samples?
FAQ ID -2526
View
Is RNA from red blood cells also isolated when RNA is extracted from a PAXgene Bone Marrow RNA Tube?
FAQ ID -2509
View
Is the 260/230 nm absorbance ratio specified for RNA derived from the PAXgene Bone Marrow RNA System?
FAQ ID -2514
View
Which version of the Rotor-Gene Q software can be used to run the Investigator Quantiplex?
FAQ ID -2568
View
What is the maximum tissue size that can be fixed in a PAXgene Tissue Container?
FAQ ID -2518
View
Why do I observe a CT shift for the Internal Control in some samples in comparison to the Ct value for the Internal Control of the dilution series of the Control DNA Z1?
FAQ ID -2575
View
What RNA yields are expected with the PAXgene Bone Marrow RNA System?
FAQ ID -2506
View
What is the RT-PCR performance of RNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissue compared to RNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?
FAQ ID -2538
View
What is the nature of the heterologous Internal Amplification Control and Positive Control in the mericon Assay kits?
FAQ ID -2543
View
What is the expected amplicon size when using the mericon Assay kits?
FAQ ID -2544
View
What is the longest period you recommend to leave out the dilute HiPerFect HTS at RT before use?
FAQ ID -2560
View
What is the typical amount of tissue used for disruption with the TissueLyser LT?
FAQ ID -2552
View
What kind of sample tubes are recommended to use with the TissueLyser LT?
FAQ ID -2553
View
What is the purity of nucleic acids extracted with the PAXgene Tissue kits?
FAQ ID -2533
View
Can the PAXgene Bone Marrow RNA System be used to isolate genomic DNA or viral RNA and DNA?
FAQ ID -2507
View
Can the PAXgene Bone Marrow RNA System be used with animal bone marrow or with blood samples?
FAQ ID -2501
View
Can the PAXgene RNA stabilization solution/bone marrow mixture from the PAXgene Bone Marrow RNA Tube be disposed of by adding bleach?
FAQ ID -2515
View
Can sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for other histochemical staining techniques, such as PAS?
FAQ ID -2530
View
Can the PAXgene Bone Marrow RNA System be used for other human body fluids than bone marrow samples or cultured cells of human origin?
FAQ ID -2502
View
Can the sample tubes and the TissueLyser LT Adapter be cooled with liquid nitrogen?
FAQ ID -2549
View
Can the TissueLyser LT Adapter be removed for cleaning?
FAQ ID -2546
View
Can the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit be used with hybridization probes?
FAQ ID -2595
View
Can the samples on the TissueLyser LT be protected from degradation of nucleic acids or proteins?
FAQ ID -2548
View
When using HiPerFect HTS Reagent is it necessary to optimize amount of siRNA and transfection reagent?
FAQ ID -2556
View
When using the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit, what should the fluorescent label of the probe be for the customer-defined assay to detect the pathogen?
FAQ ID -2594
View
Which human target is used for the quantification in the Investigator Quantiplex Kit?
FAQ ID -2577
View
What sample numbers can be processed on the TissueLyser LT?
FAQ ID -2547
View
What size bead should I use for tougher samples with the TissueLyser LT?
FAQ ID -2554
View
When performing the Internal Control assay for the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit, what channel or filter can be used to detect MAX?
FAQ ID -2597
View
Why is the fluorescence signal of the Internal Control on my Rotor-Gene Q very low?
FAQ ID -2574
View
Which version of the Applied Biosystems 7500 Real-Time PCR System software can be used to run the Investigator Quantiplex?
FAQ ID -2569
View
Can specific types of white blood cells be enriched from a PAXgene Bone Marrow RNA Tube prior to the RNA isolation?
FAQ ID -2508
View
Are there any accessories required for sample disruption with the TissueLyser LT?
FAQ ID -2551
View
At what temperature should I store the Investigator Quantiplex Kit?
FAQ ID -2578
View
Can bone marrow aliquots other than 2 mL be pipetted into the PAXgene Bone Marrow RNA Tube?
FAQ ID -2503
View
Are the Internal Controls used in the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit homologous to a known target?
FAQ ID -2598
View
Can I use water to elute the RNA in the PAXgene Bone Marrow RNA Kit?
FAQ ID -2512
View
Can bone marrow from leukemia patients with elevated numbers of white blood cells be processed with the PAXgene Bone Marrow RNA System?
FAQ ID -2510
View
Can PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for in situ hybridization?
FAQ ID -2529
View
How long is the fixation time?
FAQ ID -2519
View
Can HiPerFect HTS be used for miRNA?
FAQ ID -2561
View
Can HiPerFect HTS Reagent be used for transfecting shRNAs?
FAQ ID -2559
View
How long does the sample disruption with the TissueLyser LT take?
FAQ ID -2550
View
How many times can the Internal Controls used in the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit be freeze-thawed?
FAQ ID -2599
View
How long can I store the dilutions of the Control DNA Z1 standards at 2°C?
-8
View
How long can primer and probes from the mericon Assay kits be kept at°C once reconstituted?
-20
View
How should the PAXgene Bone Marrow RNA Tubes be frozen after collection and for how long?
FAQ ID -2505
View
How long does an Investigator Quantiplex run take?
FAQ ID -2566
View
How much time may the aspirated bone marrow sample be allowed to incubate before pipetting it into the PAXgene Bone Marrow RNA Tube?
FAQ ID -2504
View
I routinely heat an aliquot of the RNA eluate prior to my RT reaction. Do I still need to heat the eluate after the PAXgene Bone Marrow RNA preparation?
FAQ ID -2513
View
Do I have to calibrate new dyes on my Applied Biosystems 7500 or 7500 Fast Real-Time PCR System?
FAQ ID -2571
View
Do you have FACS or Fluoro-Microscopy data using HiPerFect HTS Reagent for delivery of siRNA?
FAQ ID -2557
View
How can I streamline the quantification workflow for the Investigator QuantiPlex Kit?
FAQ ID -2579
View
How is stability and performance of these mericon Assay kits guaranteed?
FAQ ID -2542
View
How long are the target sequences of Investigator Quantiplex?
FAQ ID -2576
View
For which real-time cyclers has the Investigator Quantiplex Kit been validated?
FAQ ID -2567
View
How are the supernatants resulting from the first two spin steps of the PAXgene Bone Marrow RNA kit purification procedure disposed?
FAQ ID -2516
View
View
What is the part number for O-rings for QIAsymphony SP and AS instruments? How often should the O-rings be replaced?
FAQ ID -3055
View
Where can I find the ordering information for QIAcube accessories such as Shaker Rack plugs, Rack labeling strips, Reagent bottle rack, 30mL Reagent bottles, Rotor Adapter holder, Rotor adapters, Sample tubes CB, Sample tubes RB and Spin column adapter rings?
FAQ ID -3054
View
Why are two reagents used in the PAXgene Tissue System?
FAQ ID -3022
View
What is the maximum size of tissue that can be fixed in a PAXgene Tissue FIX Container (50 ml)?
FAQ ID -3024
View
5. What is the maximum size of the tissue sample that can be fixed in a PAXgene Tissue Container?
FAQ ID -3023
View
What happens if a tissue sample larger than 20 x 20 x 20 mm is used for fixation with the PAXgene Tissue FIX Container (50 ml)?
FAQ ID -3025
View
Is it possible to fix over a weekend?
FAQ ID -3027
View
How long is the fixation time when using PAXgene Tissue FIX Container?
FAQ ID -3026
View
How long can a tissue specimen stay in the PAXgene Tissue STABILIZER?
FAQ ID -3028
View
What is the minimum incubation time required when using the PAXgene Tissue STABILIZER?
FAQ ID -3029
View
Is it possible to integrate the PAXgene Tissue STABILIZER into the processing?
FAQ ID -3037
View
Is it possible to archive PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3038
View
Is the morphology after H&E staining comparable to formalin-fixed samples?
FAQ ID -3039
View
Are immunohistochemistry (IHC) assays developed for formalin-fixed, paraffin-embedded (FFPE) compatible with PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3040
View
Is it possible to use formalin-fixed, paraffin-embedded (FFPE) kits and protocols for extraction of biomolecules from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3043
View
is the purity of nucleic acids isolated with the PAXgene Tissue Kits?
FAQ ID -3044
View
What is the average RNA yield from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3045
View
What is the integrity of RNA from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3046
View
What is the RT-PCR performance of RNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue compared to RNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?
FAQ ID -3050
View
How can proteins purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be analyzed?
FAQ ID -3052
View
What is the PCR performance of DNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue compared to DNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?
FAQ ID -3051
View
What is the DNA integrity from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3047
View
Does the PAXgene Tissue RNA Kit co-purify small RNAs?
FAQ ID -3048
View
How can I extract proteins from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3049
View
Can PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for in situ hybridization?
FAQ ID -3042
View
What tubes can be used with TissueLyser Adapter Set 2 x 96 (cat. no. 69984)?
FAQ ID -3086
View
What is the concentration of the Gentra RNase A that is used with the Autopure and Puregene protocols?
FAQ ID -3083
View
What are the typical yields obtained from Buccal swabs on BioSprint 96?
FAQ ID -3071
View
Where can I find a copy of the .kpf file for a BioSprint protocol?
FAQ ID -3073
View
Can the DyeEx 96 kit be used to clean sequencing reactions to be run on ABI 3730?
FAQ ID -3075
View
Can the FastLane RT reaction be incubated longer than 30 min at 42 deg C?
FAQ ID -3079
View
Does carrier RNA interfere with the QuantiTect Whole Transcriptome amplification?
FAQ ID -3080
View
What are the advantages and disadvantages of turning on the tip “re-use” feature on the QIAgility?
FAQ ID -3064
View
How precise is the QIAgility?
FAQ ID -3063
View
How long does it take to set up a 96-well plate PCR reaction on the QIAgility?
FAQ ID -3066
View
Can I use a bar code scanner or reader on the QIAgility?
FAQ ID -3067
View
Can I use a USB drive to transfer protocols to BioSprint?
FAQ ID -3072
View
Can BioSprint 96 be connected to LIMS?
FAQ ID -3070
View
How can I find the run parameters for a BioSprint protocol that I am running?
FAQ ID -3069
View
Can the FastLane Cell lysates (containing stabilized RNA, step 8. in Fastlane protocol) be stored?
FAQ ID -3077
View
Can I use specific primers with the FastLane Kits?
FAQ ID -3078
View
What is the expected level of endotoxins in plasmid DNA purified with QIAGEN Plasmid kits, QIAfilter Plasmid kits and HiSpeed Plasmid purification kits?
FAQ ID -3082
View
What is the expected level of endotoxins in plasmid DNA purified with QIAprep spin miniprep kit?
FAQ ID -3081
View
What is the concentration of the different Proteinase K solutions sold by QIAGEN?
FAQ ID -3084
View
Where can I access Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit?
FAQ ID -3085
View
Is it possible to use a standard processor — the kind used routinely for formalin-fixed samples — for dehydration and paraffin infiltration of PAXgene Tissue treated samples?
FAQ ID -3032
View
Are PAXgene Tissue Containers suitable for long term-storage at freezing temperatures?
FAQ ID -3030
View
Is it possible to manually process PAXgene Tissue treated samples without using an automated processor?
FAQ ID -3036
View
Is a special processing protocol needed?
FAQ ID -3035
View
Is it possible to process formalin-fixed and PAXgene Tissue fixed samples together in one run?
FAQ ID -3033
View
Is it necessary to clean a processor normally used for formalin-fixed tissue before using it with PAXgene Tissue fixed tissue?
FAQ ID -3034
View
Can sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for other histochemical staining techniques, such as PAS?
FAQ ID -3041
View
When should the N2 bottle used with the QIAxcel Advanced System be changed?
FAQ ID -3001
View
Are the multicopy reference assays for Type-it CNV PCR +qC Kits and the qBiomarker assays the same?
FAQ ID -3000
View
How should I reconstitute the lyophilized RNase-Free DNase Set with the RNeasy 96 kit?
FAQ ID -3003
View
How do I clean up RNA preparations containing miRNA?
FAQ ID -3002
View
What is the thermal output for a BioRobot MDx and BioRobot Universal?
FAQ ID -3056
View
What is the thermal output for a QIAsymphony SP and AS instrument?
FAQ ID -3057
View
Can I use Tungsten Carbide beads for RNA extraction protocols using QIAGEN Kits?
FAQ ID -3053
View
What is the minimum number of samples that can be extracted on a BioSprint 96?
FAQ ID -3076
View
What is the fixation method used in the PAXgene Tissue System?
FAQ ID -3019
View
What does PAXgene Tissue FIX contain?
FAQ ID -3020
View
Can I perform the IDH 1/2 RGQ PCR test with slides containing less than 40% tumor cells?
FAQ ID -3098
View
What volume of bacterial culture can be processed using the QuickLyse Miniprep Kit?
FAQ ID -1357
View
Can the MaXtract High Density Tube be used with QIAzol to isolate RNA?
FAQ ID -1304
View
I have a Human Druggable Genome siRNA Set V2.0. Can I reorder the siRNAs from this set?
FAQ ID -1366
View
What factors determine if denatured proteins accumulate underneath, or inside the gel of the MaXtract Low and High Density Tubes?
FAQ ID -1312
View
If both MaXtract Low Density and High Density is suitable for an application, which type is preferable to use?
FAQ ID -1310
View
How will service work carried out on my QIAGEN BioRobot be documented?
FAQ ID -1331
View
If I cannot order FlexiPlate siRNA online, how do I order?
FAQ ID -1371
View
What information about my gene or siRNA do I need to order FlexiPlate siRNA?
FAQ ID -1365
View
Is it possible to use MaXtract for an organic extraction with a mixture of phenol and BCP (1 bromine chlorpropane)?
-3
View
What information is provided with FlexiPlate siRNAs?
FAQ ID -1363
View
Is it possible to elute plasmid DNA obtained with the QuickLyse Miniprep Kit in nuclease-free water?
FAQ ID -1355
View
Which formats are available for FlexiPlate siRNA?
FAQ ID -1358
View
Which parts are exchanged during a Preventive Maintenance visit for my QIAGEN BioRobot?
FAQ ID -1334
View
When isolating DNA from plant cells using CTAB, should MaXtract Low or High Density be used?
FAQ ID -1308
View
Which controls can I choose for FlexiPlate siRNA?
FAQ ID -1368
View
Will the REPLI-g UltraFast Mini Kit reaction always yield 10 µg DNA?
FAQ ID -1328
View
Why is there DNA in the no-template control reaction when using the standard REPLI-g procedure, but not when using the UltraFast procedure?
FAQ ID -1327
View
Which density gel type is contained in the yellow or green MaXtract Tubes?
FAQ ID -1315
View
What is the composition of FlexiPlate siRNA buffer?
FAQ ID -1361
View
Which software updates are included in a Premium Cover Agreement for my QIAGEN Instrument?
FAQ ID -1336
View
What is the composition of elution buffer QLE in the QuickLyse Miniprep Kit?
FAQ ID -1356
View
Can I reduce the amount of starting material below 10 ng when using the REPLI-g UltraFast Mini Kit?
FAQ ID -1325
View
Can MaXtract tubes be autoclaved prior to use?
FAQ ID -1305
View
Can I reduce the reaction time to e.g., 30 minutes, when using the REPLI-g UltraFast Mini Kit?
FAQ ID -1326
View
Can I use any type of DNA extraction method to purify template DNA for amplification using the REPLI-g Mitochondrial DNA Kit?
FAQ ID -1321
View
Can the bacterial pellet in the QuickLyse Miniprep procedure be resuspended by pipetting up and down or shortening the vortexing time?
FAQ ID -1352
View
Can several extraction steps be performed in the same MaXtract tube?
FAQ ID -1302
View
Can a phenol-chloroform ratio different from 1:1 also be used with MaXtract Low and High Density?
FAQ ID -1309
View
Can DNA isolated with MaXtract tubes be used for downstream applications such as restriction digestion, labeling, blotting, PCR, and automated sequencing?
FAQ ID -1303
View
Can I amplify mitochondrial DNA directly from whole blood or cells using the REPLI-g Mitochondrial DNA Kit?
FAQ ID -1319
View
Can I order Alexa Fluor labeled controls with FlexiPlate siRNA?
FAQ ID -1369
View
Can frozen sample material pulverized in liquid nitrogen be directly added to MaXtract tubes?
FAQ ID -1301
View
Can HP Validated siRNAs be ordered on the FlexiPlate?
FAQ ID -1367
View
Can I also screen large constructs like BACs, fosmids, and cosmids using the QIAGEN PlasmidAmp Kit?
FAQ ID -1337
View
How do I resuspend my FlexiPlate siRNAs?
FAQ ID -1360
View
How much REPLI-g amplified mitochondrial DNA should be used in a subsequent PCR reaction?
FAQ ID -1320
View
Do mutations in the target mitochondrial DNA prevent primer binding, resulting in no DNA amplification with the REPLI-g Mitochondrial DNA Kit?
FAQ ID -1323
View
Do you have stability data for genomic DNA isolated with the BioSprint Kits?
FAQ ID -1318
View
Does the addition of sample and organic solution to the MaXtract Tubes require a specific pipetting sequence?
FAQ ID -1307
View
Do you have stability data for genomic DNA isolated with the Flexigene DNA Kit?
FAQ ID -1317
View
How does a Service Support Agreement provide defined budgeting for maintaining my BioRobot?
FAQ ID -1330
View
How can I upload my gene list for FlexiPlate siRNA to GeneGlobe?
FAQ ID -1364
View
How can I increase the DNA yield from low-copy plasmids using the QuickLyse Miniprep Kit?
FAQ ID -1354
View
How can I ensure optimal performance of my QIAGEN instrument?
FAQ ID -1329
View
What is the "seed sequence" of a miRNA?
FAQ ID -2262
View
What is the turn around time for the ordering of miScript Target Protector?
FAQ ID -2254
View
What is the sample throughput of Pyrosequencing systems?
FAQ ID -2215
View
What is the processing time for 96 normalizations with the SeqTarget System?
FAQ ID -2237
View
What is the optimal range of PCR fragment sizes for normalization with the SeqTarget System?
FAQ ID -2247
View
What method do you recommend to quantify DNA before running the KRAS and EGFR PCR kit?
FAQ ID -2292
View
What technologies are used by the KRAS and EGFR PCR kits?
FAQ ID -2286
View
What kind of automation can be done with the EasyXtal 15-well Tool?
FAQ ID -2220
View
What kind of reading length can I expect when using Pyrosequencing technology for sequence analysis?
FAQ ID -2216
View
What dyes are used in the KRAS and EGFR PCR kits?
FAQ ID -2288
View
What do I do if I get amplification in my no template control (NTC) with the KRAS and EGFR PCR kits?
FAQ ID -2298
View
What format are the EasyXtal 15-well tools?
FAQ ID -2219
View
What do I need to provide when ordering a miScript Target Protector?
FAQ ID -2251
View
What is the difference of the sequence of a miScript miRNA Inhibitor and miScript Target Protector?
FAQ ID -2253
View
What is a miScript Precursor Assay?
FAQ ID -2214
View
What is the difference between probability and typicality in the Rotor-Gene ScreenClust HRM Software?
FAQ ID -2203
View
What is a miScript Target Protector?
FAQ ID -2249
View
Which transfection reagent is recommended for transfection of the miScript Target Protectors?
FAQ ID -2252
View
Which variants of the EasyXtal 15-well tool are available?
FAQ ID -2221
View
Why are most of my samples outside of the cluster in supervised mode using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2205
View
Why are some of my samples outside of the cluster using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2204
View
Why does the SeqTarget Prep Protocol require a 2-step procedure?
FAQ ID -2238
View
Why does the miScript Target Protector not induce the degradation process?
FAQ ID -2263
View
Why do I have wavy DNA bands on my agarose gel?
FAQ ID -2260
View
What will happen, if the normalized DNA for the SeqTarget System is eluted with Buffer EB instead of EB2?
FAQ ID -2242
View
When negative results are generated using the KRAS and EGFR PCR kits, do I rerun them?
FAQ ID -2294
View
What type of samples can I use for the KRAS PCR and EGFR kits?
FAQ ID -2293
View
What voltage should be used to run my agarose gel with the GelPilot Agarose?
FAQ ID -2261
View
Which mode should I use in the Rotor-Gene ScreenClust HRM Software, supervised or unsupervised?
FAQ ID -2202
View
Does the atypical look of the lyophilized FlexiTube siRNA Premix have a negative impact on performance?
FAQ ID -2267
View
Does the miScript Target Protector match the target sequence?
FAQ ID -2250
View
Do the KRAS and EGFR PCR kits contain a passive reference dye?
FAQ ID -2295
View
Do I have to order at least 4 FlexiTube siRNA Premixes?
FAQ ID -2266
View
How do I store my FlexiTube siRNA Premix?
FAQ ID -2270
View
How is ARMS technology superior to alternative methods such as dye terminator sequencing?
FAQ ID -2290
View
Effects of low A260/A230 ratios in RNA preparations on downstream applications
FAQ ID -2248
View
How can I check the success of the normalization procedure for the SeqTarget System?
FAQ ID -2245
View
How do I search for all the miScript Target Protectors for one species?
FAQ ID -2255
View
Can PCR fragments be normalized for the SeqTarget System after purification with QIAquick?
FAQ ID -2240
View
Can PCR fragments be normalized for the SeqTarget System without a previous purification step?
FAQ ID -2246
View
Can I use a 384 well plate for the KRAS and EGFR PCR kits?
FAQ ID -2296
View
Are all 3 components of the SeqTarget System required or can a different Long Range PCR Kit and primers be used?
FAQ ID -2234
View
Can the miScript Precursor Assay differentiate between pri- and pre-miRNA?
FAQ ID -2211
View
Does ethanol from the PCR product purification influence the normalization procedure for the SeqTarget System?
FAQ ID -2244
View
What are EasyXtal 15-well Tools used for?
FAQ ID -2217
View
What are Principal Components analyzed in Rotor-Gene ScreenClust HRM Software?
FAQ ID -2200
View
Is the SeqTarget System compatible with standard-size PCRs?
FAQ ID -2241
View
What are the amplicon lengths of the KRAS and EGFR PCR kits?
FAQ ID -2289
View
What are clusters analyzed in the Rotor-Gene ScreenClust HRM Software?
FAQ ID -2201
View
What does QIAGEN use for a positive control in precursor detection experiments?
FAQ ID -2212
View
What controls are used in the KRAS and EGFR PCR kits to ensure reliable results?
FAQ ID -2291
View
What do I do if I cannot see amplification in the FAM channel in my KRAS and EGFR PCR kits?
FAQ ID -2297
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What are the corresponding QIAGEN names for former Biotage instruments?
FAQ ID -2285
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What buffer conditions give the best resolution for agarose gel electrophoresis?
FAQ ID -2256
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What can be the reason for low DNA yields after normalization with the SeqTarget System?
FAQ ID -2239
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How much buffer should be used for agarose gel electrophoresis?
FAQ ID -2257
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How much DNA should be loaded per well of an agarose gel?
FAQ ID -2259
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How should gels be cast with the GelPilot Agarose so that optimal resolution is achieved?
FAQ ID -2258
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How long does it take to generate results using the KRAS and EGFR PCR kits?
FAQ ID -2299
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How long should PCR fragments be for the SeqTarget System?
FAQ ID -2236
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How many miScript Precursor Assays do you offer?
FAQ ID -2213
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Is it possible to use lower siRNA concentrations with FlexiTube siRNA Premix?
FAQ ID -2264
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Is one able to use FlexiTube siRNA Premix for transfection of primary adherent cell types?
FAQ ID -2268
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Is one able to use FlexiTube siRNA Premix for transfection of primary suspension cell types?
FAQ ID -2269
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If I am working with a difficult-to-transfect cell type or if I obtain only weak silencing effects what can I do?
FAQ ID -2265
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Is it possible to normalize to more or less than 500 ng with the SeqTarget System if needed?
FAQ ID -2235
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Is it possible to purify and normalize long range PCRs produced with PCR kits from other vendors for the SeqTarget System?
FAQ ID -2243
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Is it possible to purify viral RNA using FastLane Cell RT-PCR Kits?
FAQ ID -1484
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Why is the RT step with the QuantiFast RT Kits much shorter compared to QuantiTect RT Kits?
FAQ ID -1451
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What applications are offered on the QIAcube?
FAQ ID -1403
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Why is the activation time for HotStarTaq Plus Polymerase in the QuantiFast SYBR Green Kits different from that for QuantiFast Probe Kits?
FAQ ID -1449
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Why is the storage time for QuantiFast PCR Kits shorter than that for QuantiTect PCR Kits?
FAQ ID -1446
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Why is the reaction volume for QuantiFast PCR Kits lower than that for QuantiTect PCR Kits?
FAQ ID -1447
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Why is the QuantiFast denaturation step different for PCR and RT-PCR runs in the two-step protocol for the ABI 7500 and other cyclers?
FAQ ID -1442
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What is the minimum number of siRNAs per order? If I order 40 siRNAs, can I get those on 2 plates?
FAQ ID -1468
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Can purification columns from other suppliers be processed on the QIAcube?
FAQ ID -1406
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Can QIAcube protocol runs be interrupted, and subsequently be continued to completion?
FAQ ID -1414
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Are run reports and/or log files available on the QIAcube?
FAQ ID -1412
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Can I use more than 4 µl FastLane lysate in subsequent real-time RT-PCR?
FAQ ID -1480
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Are FlexiPlate siRNAs available at a higher final concentration than 10 µM?
FAQ ID -1476
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Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with QuantiFast Probe PCR Kits?
FAQ ID -1452
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Can different amounts of siRNA be ordered on one FlexiPlate?
FAQ ID -1473
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Can 2 µl reaction volumes be used with QuantiFast PCR Kits?
FAQ ID -1440
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Are there any specific cleaning solutions recommended for the QIAcube?
FAQ ID -1416
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Do the QuantiTect Primer Assays work with QuantiFast SYBR Green Kits?
FAQ ID -1439
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Do special settings have to be used for QuantiFast PCR Kits on the Eppendorf Mastercycler ep realplex?
FAQ ID -1437
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Do I need to buy special kits for the QIAcube?
FAQ ID -1402
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Do I need to discard partially used QIAcube tip racks?
FAQ ID -1418
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What information is required to reorder specific siRNAs from a siRNA Set in FlexiPlate format?
FAQ ID -1463
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What are the turnaround times for FlexiPlate siRNA orders?
FAQ ID -1478
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What are the dimensions and weight of the QIAcube?
FAQ ID -1407
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How much time will be saved when switching from standard cycling to fast cycling with QuantiFast Kits?
FAQ ID -1438
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How many reactions can I perform with the new QuantiFast Kits for real-time PCR?
FAQ ID -1425
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If FlexiPlate siRNA was accidentally redissolved in siRNA Suspension Buffer instead of water, resulting in a 2x buffer, would it affect performance of the siRNAs?
FAQ ID -1477
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How do you achieve fast cycling, yet still deliver the same performance in PCR as that achieved with standard cycling?
FAQ ID -1430
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Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube?
FAQ ID -1415
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Why is a 2-step (and not a 3-step) cycling protocol recommended for QuantiFast SYBR Green PCR Kits?
FAQ ID -1450
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Why do QuantiFast SYBR Green PCR Kits require such a high primer concentration?
FAQ ID -1443
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What QuantiFast Kit should be used on the Eppendorf Mastercycler ep realplex?
FAQ ID -1436
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What is the detection limit of the QuantiFast Kits for real-time PCR?
FAQ ID -1432
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Which kind of 96 and 384-well plates are available for FlexiPlate siRNAs?
FAQ ID -1459
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Which cell lines have been tested with FastLane Cell RT-PCR Kits?
FAQ ID -1485
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Can I adjust the ROX concentration in the QuantiFast master mix?
FAQ ID -1427
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Can FlexiPlate siRNAs be ordered in plates other than the standard NUNC 96 well V bottom plates?
FAQ ID -1458
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Can I follow the reaction setup for probe-based assays described in Appendix D of the FastLane Cell RT-PCR Handbook using 384-well plates?
FAQ ID -1482
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Can fast cycling be carried out with QuantiFast Probe PCR Kits and FRET probes?
FAQ ID -1433
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Can FlexiPlate siRNA be ordered in solution on dry-ice?
FAQ ID -1474
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Can I program my own protocols for the QIAcube?
FAQ ID -1405
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How can I load new protocols onto the QIAcube?
FAQ ID -1421
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How can I get software updates for the QIAcube?
FAQ ID -1413
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How do QuantiFast PCR Kits compare to QuantiTect PCR Kits for quantitative real-time PCR?
FAQ ID -1441
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Do you offer trial-kit sizes for the new QuantiFast Kits?
FAQ ID -1429
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How can I decontaminate the QIAcube system?
FAQ ID -1419
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Can the QIAcube heater/shaker be used independently from protocol runs?
FAQ ID -1422
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Can the QIAcube rotor adapters be autoclaved?
FAQ ID -1409
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Can the QIAcube centrifuge be used independently from protocol runs?
FAQ ID -1423
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Can QuantiFast PCR Kits be used on real-time PCR instruments without fast cycling options?
FAQ ID -1428
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Can the QIAcube be connected to a laboratory information management system?
FAQ ID -1411
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Can the QIAcube rotor and/or buckets be removed for cleaning?
FAQ ID -1410
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Does the high primer concentration required by QuantiFast SYBR Green PCR Kits impair annealing specificity?
FAQ ID -1444
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Does the master mix in the QuantiFast Kits contain dUTP to allow UNG treatments?
FAQ ID -1431
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Can the QuantiFast Probe PCR +ROX Vial mastermix be stored long-term after adding ROX dye?
FAQ ID -1435
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What are the Tms of PCR products obtained using the miScript System?
FAQ ID -1599
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Do you have a protocol for cleanup of REPLI-g amplified DNA?
FAQ ID -1545
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What can be done if the protein pellet does not solubilize completely using the AllPrep DNA/RNA/Protein Mini Kit?
FAQ ID -1580
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What controls should be used for normalizing miRNA real-time PCR data obtained with the miScript System?
FAQ ID -1596
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What is Q-Bond used in the QIAGEN Fast Cycling PCR and QuantiFast Kits?
FAQ ID -1554
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Will QuantiTect Primer Assays work at an annealing temperature of 60ºC with QuantiFast SYBR Green PCR and RT-PCR Kits?
FAQ ID -1553
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Which homogenization method do you recommend for tissues stored in Allprotect Tissue Reagent?
FAQ ID -1572
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Can different incubation times be used for the 2 hour amplification step in the QuantiTect Whole Transcriptome protocol?
-8
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Does residual Allprotect Tissue Reagent left behind on tissue samples interfere with DNA, RNA, and protein purification?
FAQ ID -1573
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Is cDNA generated with the QuantiTect Whole Transcriptome Kit suitable for use in microarray analysis?
FAQ ID -1587
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Do you have a protocol for purification of genomic DNA from cultured cells using the QIAamp DNA Micro Kit?
FAQ ID -1547
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Do you have a protocol for concentration of RNA in 96-well plates?
FAQ ID -1546
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Do you have a protocol for purification of total RNA from fatty tissues using QIAzol Lysis Reagent and MaXtract High Density?
FAQ ID -1550
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Which real-time PCR kits are recommended downstream of the QuantiTect Whole Transcriptome Kit?
FAQ ID -1592
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What primers are used in the reverse-transcription step of the QuantiTect Whole Transcriptome procedure?
FAQ ID -1584
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What is the maximum amount of RNA that can be used for amplification with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1591
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Can the AllPrep DNA/RNA/Protein Mini Kit be used with fibrous or lipid tissues?
FAQ ID -1579
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What starting amount of RNA purified using an miRNeasy Kit should be used with the miScript System?
FAQ ID -1597
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Can samples in Allprotect Tissue Reagent undergo repeated freeze-thaws without degradation of DNA, RNA, and protein?
FAQ ID -1575
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