The Insight Exchange on Cell Line Development
Find out the best practices in cell line development and cell and gene therapy from renowned global scientists
Overview
The vulnerabilities of cell line engineering
- Given the low success rate of gene editing techniques, screening for successfully edited clones and characterizing the edits could take several months to complete. We are here to make your cell line development process easier and faster.
- Gene editing, viral transduction or even routine transfections can cause off-target effects such as chromosomal rearrangements and structural variants, often missed by classical methods like karyotyping and FISH. Choosing your genome assessment method wisely when working with cell line development is indeed an important decision in getting the cell right.
Ready to accelerate your clone selection workflow?
Go to our dedicated page to explore the QIAprep& CRISPR kit and QuantiNova one-step RT-qPCR solutions in detail.
Don’t sacrifice speed for quality – instead, combine both in your clone selection and cell line characterization workflow
Clone selection and enrichment
Gene expression analysis – validate gene edits at the transcript level using QuantiNova PCR
Cell line characterization
Get ahead of unintended changes lurking in your cells
Go to our dedicated page and explore EpiTect Hi-C
FAQs
CRISPR screening
Is there anything to consider when processing cultured cells on coated cultivation dishes? Are additional purification steps required to eliminate or reduce coating reagents such as Poly-L-Lysine?
Is there something to consider when working with transduced cells treated with Polybrene?
Can the new CRISPR PCR Assays be used for digital PCR (dPCR) on the QIAcuity? Do you have data or a protocol?
EpiTect Hi-C
Are there stopping points in the EpiTect Hi-C workflow?
What is the effect of using amounts of input material per sample that fall outside the recommended range?
Can I use cells that have been previously crosslinked and frozen (e.g., for a ChIP or ChIP-seq experiment) as input material for the EpiTect Hi-C protocol?
With which platform can I perform my sequencing e.g. within cell line development process (CLD)?
Can I use my own pipeline to analyze results from my EpiTect Hi-C experiments e.g. in cell line development (CLD)?
With which read length should the EpiTect Hi-C NGS libraries be sequenced?
Does QIAGEN provide data analysis to accompany the EpiTect Hi-C Kit?
QuantiNova RT-PCR kit
Do I require any specific equipment to analyze a cultured cell after direct lysis using QuantiNova RT-qPCR Kits?
How many cells are required to check gene expression levels of my edited gene(s) in direct PCR?
Which cell lines are suited for direct lysis and RT-qPCR?
Is it required to wash cells before direct lysis and RT-qPCR?
Do I need to set up the RT-PCR reaction on ice?