TissueLyser Adapter Sets

For efficient disruption of up to 48 or 192 samples in parallel on the TissueLyser II

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TissueLyser Adapter Set 2 x 24

Cat. No. / ID: 69982

Two sets of adapter plates and 2 racks for use with 2 ml microcentrifuge tubes on the TissueLyser II

For

Microcentrifuge tubes

Collection microtubes and caps

TissueLyser Adapter Sets are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Product Details

TissueLyser Adapter Sets are designed for efficient and user-friendly disruption of up to 48 or 192 samples in parallel using the QIAGEN TissueLyser II.

The TissueLyser Adapter Set 2 x 24 contains a pair of TL Reaction-Tube Holders, 24-Tube. Each holder holds up to 24 microcentrifuge tubes (2 ml). The use of Safe-Lock tubes is strongly recommended (e.g., Eppendorf 2 ml Safe-Lock micro test tubes).

The TissueLyser Adapter Set 2 x 96 is for use with QIAGEN Collection Microtubes (racked) and Collection Microtube Caps. Collection Microtubes are 1.2 ml in volume.

Resources

Up to 1 x 10⁹ bacteria are disrupted and homogenized by bead-milling in a guanidine-thiocyanate-containing lysis buffer. After addition of ethanol, the sample is loaded onto an RNeasy Mini spin column. Total RNA binds to the RNeasy silica-membrane, contaminants are efficiently washed away, and high-quality RNA is eluted in RNase-free water.

For high-throughput disruption of biological samples

For efficient disruption of up to 48 or 192 samples in parallel on the TissueLyser

FAQ

We do not recommend using the adapters of the TissueLyser II with liquid nitrogen. Instead, the adapters can be precooled at –80°C, either in the freezer or on dry ice.

FAQ ID -9173

TissueLyser Adapter Set 2 x 96 should be used with Collection Microtubes (racked) and Collection Microtube Caps (cat. nos. 19560 and 19566 respectively).

FAQ ID -3086

Yes, we have the following protocols:

Purification of total RNA from fatty tissues using QIAzol Lysis Reagent, MaXtract High Density, and the TissueRuptor (RY29).
Purification of total RNA from fatty tissues using QIAzol Lysis Reagent, MaXtract High Density, and the TissueLyser (RY30).
Purification of total RNA from fatty tissues using the RNeasy Lipid Tissue Mini Kit and MaXtract High Density (RY31).

FAQ ID -1550

100 mg tissue per sample tube is the maximum amount we recommend when using the 2 x 24 Adapter Set. Larger amounts of tissue would require considered optimization, depending on the tissue type and condition.

When using 100 mg tissue, for best homogenization results, we recommend cutting the sample into 2 pieces of approximately 50 mg each prior to disruption and performing disruption twice, each time at 25 Hz for 5 min.

FAQ ID -9178