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How much buffer should be used for agarose gel electrophoresis?
FAQ ID -2257
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How much DNA should be loaded per well of an agarose gel?
FAQ ID -2259
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How should gels be cast with the GelPilot Agarose so that optimal resolution is achieved?
FAQ ID -2258
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How long does it take to generate results using the KRAS and EGFR PCR kits?
FAQ ID -2299
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How long should PCR fragments be for the SeqTarget System?
FAQ ID -2236
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How many miScript Precursor Assays do you offer?
FAQ ID -2213
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Is it possible to use lower siRNA concentrations with FlexiTube siRNA Premix?
FAQ ID -2264
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Is one able to use FlexiTube siRNA Premix for transfection of primary adherent cell types?
FAQ ID -2268
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Is one able to use FlexiTube siRNA Premix for transfection of primary suspension cell types?
FAQ ID -2269
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If I am working with a difficult-to-transfect cell type or if I obtain only weak silencing effects what can I do?
FAQ ID -2265
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Is it possible to normalize to more or less than 500 ng with the SeqTarget System if needed?
FAQ ID -2235
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Is it possible to purify and normalize long range PCRs produced with PCR kits from other vendors for the SeqTarget System?
FAQ ID -2243
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Is it possible to purify viral RNA using FastLane Cell RT-PCR Kits?
FAQ ID -1484
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Why is the RT step with the QuantiFast RT Kits much shorter compared to QuantiTect RT Kits?
FAQ ID -1451
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What applications are offered on the QIAcube?
FAQ ID -1403
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Why is the activation time for HotStarTaq Plus Polymerase in the QuantiFast SYBR Green Kits different from that for QuantiFast Probe Kits?
FAQ ID -1449
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Why is the storage time for QuantiFast PCR Kits shorter than that for QuantiTect PCR Kits?
FAQ ID -1446
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Why is the reaction volume for QuantiFast PCR Kits lower than that for QuantiTect PCR Kits?
FAQ ID -1447
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Why is the QuantiFast denaturation step different for PCR and RT-PCR runs in the two-step protocol for the ABI 7500 and other cyclers?
FAQ ID -1442
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What is the minimum number of siRNAs per order? If I order 40 siRNAs, can I get those on 2 plates?
FAQ ID -1468
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Can purification columns from other suppliers be processed on the QIAcube?
FAQ ID -1406
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Can QIAcube protocol runs be interrupted, and subsequently be continued to completion?
FAQ ID -1414
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Are run reports and/or log files available on the QIAcube?
FAQ ID -1412
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Can I use more than 4 µl FastLane lysate in subsequent real-time RT-PCR?
FAQ ID -1480
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Are FlexiPlate siRNAs available at a higher final concentration than 10 µM?
FAQ ID -1476
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Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with QuantiFast Probe PCR Kits?
FAQ ID -1452
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Can different amounts of siRNA be ordered on one FlexiPlate?
FAQ ID -1473
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Can 2 µl reaction volumes be used with QuantiFast PCR Kits?
FAQ ID -1440
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Are there any specific cleaning solutions recommended for the QIAcube?
FAQ ID -1416
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Do the QuantiTect Primer Assays work with QuantiFast SYBR Green Kits?
FAQ ID -1439
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Do special settings have to be used for QuantiFast PCR Kits on the Eppendorf Mastercycler ep realplex?
FAQ ID -1437
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Do I need to buy special kits for the QIAcube?
FAQ ID -1402
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Do I need to discard partially used QIAcube tip racks?
FAQ ID -1418
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What information is required to reorder specific siRNAs from a siRNA Set in FlexiPlate format?
FAQ ID -1463
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What are the turnaround times for FlexiPlate siRNA orders?
FAQ ID -1478
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What are the dimensions and weight of the QIAcube?
FAQ ID -1407
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How much time will be saved when switching from standard cycling to fast cycling with QuantiFast Kits?
FAQ ID -1438
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How many reactions can I perform with the new QuantiFast Kits for real-time PCR?
FAQ ID -1425
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If FlexiPlate siRNA was accidentally redissolved in siRNA Suspension Buffer instead of water, resulting in a 2x buffer, would it affect performance of the siRNAs?
FAQ ID -1477
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How do you achieve fast cycling, yet still deliver the same performance in PCR as that achieved with standard cycling?
FAQ ID -1430
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Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube?
FAQ ID -1415
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Why is a 2-step (and not a 3-step) cycling protocol recommended for QuantiFast SYBR Green PCR Kits?
FAQ ID -1450
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Why do QuantiFast SYBR Green PCR Kits require such a high primer concentration?
FAQ ID -1443
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What QuantiFast Kit should be used on the Eppendorf Mastercycler ep realplex?
FAQ ID -1436
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What is the detection limit of the QuantiFast Kits for real-time PCR?
FAQ ID -1432
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Which kind of 96 and 384-well plates are available for FlexiPlate siRNAs?
FAQ ID -1459
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Which cell lines have been tested with FastLane Cell RT-PCR Kits?
FAQ ID -1485
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Can I adjust the ROX concentration in the QuantiFast master mix?
FAQ ID -1427
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Can FlexiPlate siRNAs be ordered in plates other than the standard NUNC 96 well V bottom plates?
FAQ ID -1458
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Can I follow the reaction setup for probe-based assays described in Appendix D of the FastLane Cell RT-PCR Handbook using 384-well plates?
FAQ ID -1482
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Can fast cycling be carried out with QuantiFast Probe PCR Kits and FRET probes?
FAQ ID -1433
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Can FlexiPlate siRNA be ordered in solution on dry-ice?
FAQ ID -1474
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Can I program my own protocols for the QIAcube?
FAQ ID -1405
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How can I load new protocols onto the QIAcube?
FAQ ID -1421
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How can I get software updates for the QIAcube?
FAQ ID -1413
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How do QuantiFast PCR Kits compare to QuantiTect PCR Kits for quantitative real-time PCR?
FAQ ID -1441
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Do you offer trial-kit sizes for the new QuantiFast Kits?
FAQ ID -1429
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How can I decontaminate the QIAcube system?
FAQ ID -1419
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Can the QIAcube heater/shaker be used independently from protocol runs?
FAQ ID -1422
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Can the QIAcube rotor adapters be autoclaved?
FAQ ID -1409
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Can the QIAcube centrifuge be used independently from protocol runs?
FAQ ID -1423
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Can QuantiFast PCR Kits be used on real-time PCR instruments without fast cycling options?
FAQ ID -1428
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Can the QIAcube be connected to a laboratory information management system?
FAQ ID -1411
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Can the QIAcube rotor and/or buckets be removed for cleaning?
FAQ ID -1410
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Does the high primer concentration required by QuantiFast SYBR Green PCR Kits impair annealing specificity?
FAQ ID -1444
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Does the master mix in the QuantiFast Kits contain dUTP to allow UNG treatments?
FAQ ID -1431
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Can the QuantiFast Probe PCR +ROX Vial mastermix be stored long-term after adding ROX dye?
FAQ ID -1435
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What are the Tms of PCR products obtained using the miScript System?
FAQ ID -1599
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Do you have a protocol for cleanup of REPLI-g amplified DNA?
FAQ ID -1545
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What can be done if the protein pellet does not solubilize completely using the AllPrep DNA/RNA/Protein Mini Kit?
FAQ ID -1580
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What controls should be used for normalizing miRNA real-time PCR data obtained with the miScript System?
FAQ ID -1596
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What is Q-Bond used in the QIAGEN Fast Cycling PCR and QuantiFast Kits?
FAQ ID -1554
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Will QuantiTect Primer Assays work at an annealing temperature of 60ºC with QuantiFast SYBR Green PCR and RT-PCR Kits?
FAQ ID -1553
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Which homogenization method do you recommend for tissues stored in Allprotect Tissue Reagent?
FAQ ID -1572
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Can different incubation times be used for the 2 hour amplification step in the QuantiTect Whole Transcriptome protocol?
-8
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Does residual Allprotect Tissue Reagent left behind on tissue samples interfere with DNA, RNA, and protein purification?
FAQ ID -1573
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Is cDNA generated with the QuantiTect Whole Transcriptome Kit suitable for use in microarray analysis?
FAQ ID -1587
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Do you have a protocol for purification of genomic DNA from cultured cells using the QIAamp DNA Micro Kit?
FAQ ID -1547
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Do you have a protocol for concentration of RNA in 96-well plates?
FAQ ID -1546
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Do you have a protocol for purification of total RNA from fatty tissues using QIAzol Lysis Reagent and MaXtract High Density?
FAQ ID -1550
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Which real-time PCR kits are recommended downstream of the QuantiTect Whole Transcriptome Kit?
FAQ ID -1592
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What primers are used in the reverse-transcription step of the QuantiTect Whole Transcriptome procedure?
FAQ ID -1584
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What is the maximum amount of RNA that can be used for amplification with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1591
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Can the AllPrep DNA/RNA/Protein Mini Kit be used with fibrous or lipid tissues?
FAQ ID -1579
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What starting amount of RNA purified using an miRNeasy Kit should be used with the miScript System?
FAQ ID -1597
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Can samples in Allprotect Tissue Reagent undergo repeated freeze-thaws without degradation of DNA, RNA, and protein?
FAQ ID -1575
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Can the QuantiFast Probe PCR +ROX Vial Kit be used on the ABI PRISM 7900 and Applied Biosystems 7500 cyclers?
FAQ ID -1555
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Can RNA purified from formalin-fixed, paraffin-embedded (FFPE) tissue be used with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1583
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Does Buffer APP in the AllPrep DNA/RNA/Protein Mini Kit contain acetone?
FAQ ID -1576
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Can tissue homogenized in Buffer RLT Plus instead of Buffer RLT be used with the AllPrep DNA/RNA/Protein Mini Kit?
FAQ ID -1577
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Does cDNA amplified with the QuantiTect Whole Transcriptome Kit correspond to full-length RNA transcripts?
FAQ ID -1585
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Can tissue incubated in Allprotect Tissue Reagent be used for morphological studies?
FAQ ID -1571
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Can less than 10 ng RNA be used with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1590
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Can Allprotect Tissue Reagent be used to stabilize RNA, DNA and proteins in cells, bacteria, yeast, and plants?
FAQ ID -1574
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Can degraded RNA be used with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1586
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How much cDNA is sufficient for real-time PCR detection of miRNA transcripts generated with the miScript System?
FAQ ID -1598
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How many samples can be run in parallel with the Rotor-Gene Q real-time PCR instrument?
FAQ ID -1519
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What is the advantage of EpiTect MethyLight PCR Kits over QuantiTect Probe PCR Kits for methylation studies?
FAQ ID -2010
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What is the advantage of using the Type-it Microsatellite PCR Kit?
FAQ ID -2059
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What is the advantage of using the Type-it Mutation Detect PCR Kit?
FAQ ID -2064
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What is a good starting amount of template DNA for PCR with the EpiTect MSP Kit?
FAQ ID -2005
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What is elution Buffer ATE of the QIAsymphony DNA Investigator Kit?
FAQ ID -2029
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What is the maximum lifetime of the QIAGEN rotor for the Centrifuge 4 and 4K?
-16
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What is the maximum DNA yield from forensic samples processed with the QIAsymphony SP?
FAQ ID -2028
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What is the principle behind the DNA Polymerase in the EpiTect MSP Kit?
FAQ ID -2003
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Is it possible to use different sample types in one run with the MagAttract 96 cador Pathogen Kit?
FAQ ID -2072
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What is the purpose of the unmethylated and unconverted control DNA of the EpiTect PCR Control DNA Set?
FAQ ID -2007
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Is magnetic particle carry-over in the eluates on the BioSprint 96 normal?
FAQ ID -2073
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What happens when using EpiTect MethyLight Assays on only partially bisulfite converted DNA template?
FAQ ID -2009
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Should sample lysate volumes be exactly 200 µl, 500 µl, or 1000 µl when loaded on the QIAsymphony SP?
FAQ ID -2031
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What are the expected PCR results when using EpiTect Control DNA on untreated or bisulfite-converted DNA?
FAQ ID -2011
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What are the features of the expression vector used for QIAgenes Insect/Mammalia?
FAQ ID -2050
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Why do casework-sample protocols for the QIAsymphony DNA Investigator Kit start with different lysate volumes (200 µl, 500 µl, or 1000 µl)?
FAQ ID -2035
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Why is CoralLoad included in the Type-it Mutation Detect, but not in the Type-it Microsatellite PCR Kit?
FAQ ID -2068
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Why is there more than one QIAgene for my gene of interest?
FAQ ID -2044
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Why do pQE-T7 vectors of the QIAgenes E. coli system have an additional UAG amber stop codon?
FAQ ID -2043
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Why is the Type-it Mutation Detect PCR Kit recommended for preamplification of SNPs?
FAQ ID -2067
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Will the optimized DNA sequence for QIAgenes be provided?
FAQ ID -2045
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Why is maximum amplicon size for the Type-it Microsatellite PCR Kit limited to 500 bp?
FAQ ID -2060
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Why is special Type-it Fast SNP Probe PCR chemistry required for TaqMan® SNP Genotyping?
FAQ ID -2057
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What sample volumes can be processed with the QIAsymphony Virus/Bacteria Kits?
FAQ ID -2037
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What sample types can be processed using the QIAsymphony Virus/Bacteria protocols?
FAQ ID -2036
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Can data be analyzed on the Rotor-Gene Q while a run is in progress?
FAQ ID -2091
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Are there two vectors for protein expression using QIAgenes in insect or mammalian cells?
FAQ ID -2048
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Are there recommendations for sensitive detection of underrepresented fragments using the Type-it Mutation Detect PCR Kit?
FAQ ID -2066
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Can other probe technologies besides TaqMan® be used with the Type-it Fast SNP Probe PCR Kit?
FAQ ID -2058
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Is mitochondrial DNA isolated with the QIAsymphony DNA Investigator Kit on the QIAsymphony SP?
FAQ ID -2030
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Is a passive internal reference dye, like ROX, required on the Rotor-Gene Q to obtain reproducible results?
FAQ ID -2079
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Is it possible to edit the sample sheet in the Rotor-Gene Q software after a PCR run?
FAQ ID -2092
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Is it possible to import a standard curve from a previous PCR run on the Rotor-Gene Q?
FAQ ID -2093
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What are the ideal and the largest PCR amplicon sizes when using the EpiTect MSP Kit?
FAQ ID -2004
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What kind of samples should be processed using the large-volume protocol with the QIAsymphony DNA Investigator Kit?
FAQ ID -2034
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Which expression systems can be used with the QIAgenes Insect/Mammalia expression constructs?
FAQ ID -2049
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Which QIAsymphony Virus/Bacteria Kit size should be used for each protocol?
FAQ ID -2038
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What types of reaction vessels are required for use in the Rotor-Gene Q?
FAQ ID -2085
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Which capillary sequencers can be used for detection of amplicons from the Type-it Microsatellite PCR Kits?
FAQ ID -2063
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Can the EpiTect PCR Control DNAs be used for mouse and rat assays?
FAQ ID -2008
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Can the EpiTect PCR Control DNAs also be used as standard in real-time methylation specific quantification?
FAQ ID -2006
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Can the protocols for purification of small RNAs from cells in the appendices of the AllPrep DNA/RNA 96 Handbook also be used with tissue samples?
FAQ ID -2001
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Can solid samples be processed with the QIAsymphony DNA Investigator Kit on the instrument?
FAQ ID -2027
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Can QIAGEN rotors used with the centrifuges 4C and 4KC also be used with 4-16 and 4-16K models?
-15
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Can the MagAttract 96 cador Pathogen Kit also be used on the KingFisher 96 or MagMAX Express 96 instruments?
FAQ ID -2074
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Do all QIAsymphony DNA Investigator protocols use carrier RNA?
FAQ ID -2033
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Can TopTaq Master Mix simply be stored at room temperature on the lab bench for up to 4 months?
FAQ ID -2052
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Can the Type-it Mutation Detect PCR kit be used for multiplex PCR of fragments longer than 500 bp?
FAQ ID -2065
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Can the Type-it Microsaltellite PCR Kit be used on cyclers with fast ramping rates?
FAQ ID -2062
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Can 0.1 ml and 0.2 ml tubes used on the Rotor-Gene Q be labeled?
FAQ ID -2082
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How many signal readings are taken during the real-time data acquisition on the Rotor-Gene Q?
FAQ ID -2083
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Can membrane proteins be expressed with QIAgenes expression kits?
FAQ ID -2047
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Can DNase digestion be performed on the RNeasy 96 plate during the RNA purification part of the Allprep DNA/RNA 96 procedure?
FAQ ID -2000
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Does an internal control have to be included for each QIAsymphony Virus/Bacteria protocol run?
FAQ ID -2040
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Do QIAsymphony Virus/Bacteria samples require pretreatment before they are loaded onto the instrument?
FAQ ID -2039
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Does the centrifugal force have any effect on the kinetics of a PCR reaction on the Rotor-Gene Q?
FAQ ID -2081
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How can a C-terminal 6xHis-tagged QIAgene be expressed?
FAQ ID -2041
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How can full-length genes or individual domains of the QIAgenes Insect/Mammalia constructs be subcloned?
FAQ ID -2051
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Does the Rotor-Gene Q software have the capacity to export data?
FAQ ID -2089
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Can DNA yields be increased by prolonging incubation with proteinase K in Buffer ATL?
FAQ ID -2032
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Can frozen samples be used with the MagAttract 96 cador Pathogen Kit?
FAQ ID -2070
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How fast does the rotor spin during a run on the Rotor-Gene Q?
FAQ ID -2080
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How is the log graph threshold value (Ct) for the Rotor-Gene Q determined?
FAQ ID -2088
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How long can DNA converted with EpiTect Bisulfite Kits be stored before use in methylation specific PCR with the EpiTect MSP Kit?
FAQ ID -2002
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How does the built-in QC control for complete bisulfite conversion of DNA work on PyroMark Q24?
FAQ ID -2095
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How will running less than 96 samples with the MagAttract 96 cador Pathogen kit affect usage of plasticware?
FAQ ID -2071
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If orders cannot be placed online, how can QIAgenes be ordered?
FAQ ID -2046
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If new dyes or chemistries appear on the real-time PCR market, will Rotor-Gene Q be capable of running those dyes?
FAQ ID -2087
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How long does a single run on the PyroMark Q24 take?
FAQ ID -2096
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How much space does the PyroMark Q24 instrument need?
FAQ ID -2098
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Does the Type-it Microsatellite PCR Kit always require an annealing temperature of 60°C, or can pre-optimized annealing temperatures for target-primer systems be used?
FAQ ID -2061
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How accurate and reliable is PyroMark Q24 in mutation analysis?
FAQ ID -2094
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Is there a charge for user training on the QIAsymphony SP System?
FAQ ID -1900
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Is the QIAsymphony RNA Kit compatible with stabilized samples?
FAQ ID -1944
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Is sample pretreatment required for purifying RNA on the QIAsymphony SP?
FAQ ID -1938
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Is RNA co-purified with DNA using QIAsymphony DNA Tissue protocols?
FAQ ID -1920
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What are the expected DNA yields from tissue processed on the QIAsymphony SP?
FAQ ID -1919
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QuantiTect Primer Assays are bioinformatically validated, genomewide primer sets. What does “bioinformatically validated” mean?
FAQ ID -1982
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What are the dimensions and weight of the QIAsymphony SP System?
FAQ ID -1901
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What is the range of elution volumes on the QIAsymphony SP?
FAQ ID -1908
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How many PCR reactions can be performed for different scales of miScript Primer Assays?
FAQ ID -1990
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How many samples can be processed per kit or per reagent cartridge for RNA purification on the QIAsymphony SP?
FAQ ID -1940
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How does QIAsymphony SP keep cellular samples in suspension prior to pipetting so that samples do not settle?
FAQ ID -1905
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How efficient is DNA removal by a gDNA Eliminator 96 plate compared with DNase digestion on an RNeasy 96 plate?
FAQ ID -1994
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How long can homogenates remain on the QIAsymphony SP without risk of RNA degradation?
FAQ ID -1941
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How much sample can be used with the Allprep DNA/RNA 96 Kit?
FAQ ID -1997
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How much sample can be used with the RNeasy Plus 96 Kit?
FAQ ID -1992
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How much starting material can be used with the QIAsymphony DNA Mini Kit on the QIAsymphony SP?
FAQ ID -1917
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What sample types can be used with the AllPrep DNA/RNA 96 Kit?
FAQ ID -1996
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Which types and amounts of sample material can be processed for RNA extraction on the QIAsymphony SP?
FAQ ID -1937
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What sample types can be used with the RNeasy Plus 96 Kit?
FAQ ID -1991
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Will mitochondrial DNA also be isolated using the DNA Tissue protocols on the QIAsymphony SP?
FAQ ID -1921
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What sample types should be processed with the High Content (HC) protocol for DNA extraction on the QIAsymphony SP?
FAQ ID -1918
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Why does the Allprep DNA/RNA 96 Kit use Buffer RLT, whereas the AllPrep DNA/RNA Mini Kit uses Buffer RLT Plus?
FAQ ID -1999
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Why is there no vacuum-only protocol for the RNeasy Plus 96 Kit?
FAQ ID -1993
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What is the turn around time for custom miScript miRNA mimics, inhibitors and primer assays?
FAQ ID -1985
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Is it possible to modify QIAsymphony protocols?
FAQ ID -1903
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What are the starting volumes of homogenate for RNA extraction on the QIAsymphony SP?
FAQ ID -1939
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What are the expected yields of total RNA isolated with the QIAsymphony SP?
FAQ ID -1942
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What kind of molecules are miScript miRNA Inhibitors?
FAQ ID -1983
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What sample input volumes can be used on the QIAsymphony SP?
FAQ ID -1907
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What sample types can be processed using the tissue protocols for DNA extraction on the QIAsymphony SP?
FAQ ID -1915
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What kind of molecules are miScript miRNA Mimics?
FAQ ID -1986
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What RNA elution volumes are available with the QIAsymphony RNA Kit?
FAQ ID -1943
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Do samples require pretreatment before loading onto the QIAsymphony SP for DNA extraction?
FAQ ID -1916
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Do limiting primer concentrations need to be determined when using QuantiFast Multiplex PCR Kits?
FAQ ID -1976
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Can uneven sample numbers be processed on the QIAsymphony SP module?
FAQ ID -1906
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Does the QIAsymphony SP system generate run reports and/or log files?
FAQ ID -1911
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Does HiPerFect work for the transfection of miScript miRNA mimics and inhibitors?
FAQ ID -1984
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Do you have a protocol for the purification of archive-quality DNA from 0.5–20 mg paraffin-embedded tissue using Gentra Puregene Kits?
FAQ ID -1964
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Do you have a protocol for the purification of archive-quality DNA from 1 ml of whole blood with the Gentra Puregene Tissue Kit?
FAQ ID -1953
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Do you have a protocol for the purification of archive-quality DNA from 100 to 5 x 10e8 cultured cells using Gentra Puregene Kits?
FAQ ID -1960
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Do you have a protocol for rapid purification of archive-quality DNA from up to 9 x 10e6 cells using Gentra Puregene Kits?
FAQ ID -1969
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Can I program my own protocols for the QIAsymphony SP?
FAQ ID -1904
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Are QuantiFast Multiplex PCR Kits available in trial sizes?
FAQ ID -1981
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Are there any specific cleaning solutions recommended for the QIAsymphony SP?
FAQ ID -1913
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Can 4-plex, real-time PCR be performed on the ABI PRISM 7000, 7700, or 7900 using the QuantiFast Multiplex PCR Kit?
FAQ ID -1978
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Can the QIAsymphony RNA Kit be used to purify RNA from blood?
FAQ ID -1945
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Can the QIAsymphony SP Module be connected to a laboratory information management system (LIMS)?
FAQ ID -1909
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Can protocol runs on the QIAsymphony SP be interrupted and resumed?
FAQ ID -1912
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Can the QuantiFast Multiplex PCR Kits be used on Roche LightCycler systems with TaqMan® probes?
FAQ ID -1979
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Can the protocols for purification of small RNAs from cells in the appendices of the RNeasy Plus 96 Handbook also be used with tissue samples?
FAQ ID -1995
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Do you have a protocol for the purification of archive-quality DNA from marine invertebrate tissue using Gentra Puregene Kits?
FAQ ID -1950
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Do you have a protocol for the purification of archive-quality DNA from nematodes using Gentra Puregene Kits?
FAQ ID -1951
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Do you have a protocol for the purification of archive-quality DNA from human dried blood using Gentra Puregene Kits?
FAQ ID -1959
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Do you have a protocol for the purification of archive-quality DNA from Gram-negative bacteria using Gentra Puregene Kits?
FAQ ID -1975
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Do you have a protocol for the purification of archive-quality DNA from Gram-positive bacteria using Gentra Puregene Kits?
FAQ ID -1946
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Do you have a protocol for the purification of archive-quality DNA from hair roots using Gentra Puregene Kits?
FAQ ID -1947
浏览
Do you have a protocol for the purification of DNA from fruit flies?
FAQ ID -1970
浏览
Do you have a protocol for the purification of archive-quality DNA from tissue fixed in ethanol or formalin using Gentra Puregene Kits?
FAQ ID -1974
浏览
How can the QIAsymphony SP system be decontaminated?
FAQ ID -1914
浏览
Do you have a protocol for the purification of archive-quality DNA from umbilical cord blood using Gentra Puregene Kits?
FAQ ID -1958
浏览
Do you have a protocol for the purification of archive-quality DNA from buffy coat using Gentra Puregene Kits?
FAQ ID -1955
浏览
Do you have a protocol for the purification of archive-quality DNA from whole blood using Gentra Puregene Kits?
FAQ ID -1954
浏览
Do you have a protocol for the purification of archive-quality DNA from cerebrospinal fluid (CSF) using Gentra Puregene Kits?
FAQ ID -1967
浏览
Do you have a protocol for the purification of archive-quality DNA from Chlamydomonas using Gentra Puregene Kits?
FAQ ID -1968
浏览
Do you have a protocol for the purification of archive-quality DNA from blood smears using Gentra Puregene Kits?
FAQ ID -1948
浏览
Do you have a protocol for the purification of archive-quality DNA from fecal cells using Gentra Puregene Kits?
FAQ ID -1972
浏览
Do you have a protocol for the purification of archive-quality DNA from fish using Gentra Puregene Kits?
FAQ ID -1973
浏览
Do you have a protocol for the purification of archive-quality DNA from fungus using Gentra Puregene Kits?
FAQ ID -1971
浏览
Do you have a protocol for the purification of archive-quality DNA from buccal brushes using Gentra Puregene Kits?
FAQ ID -1965
浏览
Do you have a protocol for the purification of archive-quality DNA from Eimeria oocysts using Gentra Puregene Kits?
FAQ ID -1952
浏览
What effect does homogenization have on DNA yield and integrity when using AllPrep DNA/RNA Kits?
FAQ ID -1751
浏览
What is the fidelity of TopTaq DNA Polymerase?
FAQ ID -1739
浏览
Is TopTaq DNA Polymerase a hot-start polymerase?
FAQ ID -1748
浏览
Which nucleotide analogs can be used with TopTaq DNA Polymerase?
FAQ ID -1741
浏览
Why might Affymetrix GeneChip Mapping assays interfere with the REPLI-g FFPE kit?
FAQ ID -1755
浏览
What is the upper length of PCR products that can be amplified with TopTaq DNA Polymerase and Master Mix Kits?
FAQ ID -1738
浏览
What is the shelf-life of TopTaq DNA Polymerase and Master Mix?
FAQ ID -1743
浏览
What is the principle of the REPLI-g FFPE Kit?
FAQ ID -1753
浏览
What is the largest plasmid size that can be purified using the QuickLyse Miniprep Kit?
FAQ ID -1752
浏览
What is the minimum amount of starting material that can be used with the RNeasy Plus Micro and AllPrep DNA/RNA Micro Kit?
FAQ ID -1750
浏览
Can small miRNA-containing RNA fractions be separated from large RNAs using the miRNeasy FFPE Kit?
FAQ ID -1737
浏览
What is the recommended sample size for use with the REPLI-g FFPE Kit?
FAQ ID -1756
浏览
Can miRNA and other RNAs smaller than 200 nucleotides be isolated with the AllPrep DNA/RNA Micro Kit or RNeasy Plus Micro Kit?
FAQ ID -1749
浏览
Are there problems with the default threshold setting on the Applied Biosystems 7500 when using QuantiFast Probe PCR Kits?
FAQ ID -1702
浏览
Can TA/UA cloning be done with PCR products amplified with TopTaq DNA Polmyerase and TopTaq Master Mix Kits?
FAQ ID -1740
浏览
At which step in the REPLI-g FFPE protocol should PicoGreen quantification be done?
FAQ ID -1758
浏览
Have QuantiTect Primer Assays been tested with QuantiFast SYBR Green PCR Kits on the Mastercycler ep realplex?
FAQ ID -1714
浏览
How can foaming of Buffer RLT Plus lysates be avoided when using RNeasy Plus Micro and Mini Kits, or the Allprep DNA/RNA Mini Kit?
FAQ ID -1734
浏览
Do you have protocols for sample processing on the Autopure LS instrument?
FAQ ID -1759
浏览
Can the REPLI-g FFPE procedure restore DNA derived from FFPE tissue samples in the original order?
FAQ ID -1754
浏览
Can the REPLI-g FFPE Kit also be used for formalin-fixed, ethanol preserved samples?
FAQ ID -1760
浏览
Do you have a protocol for the isolation of total RNA from paraffin-embedded sections on the Biorobot M48?
FAQ ID -1733
浏览
Does TopTaq Stabilizer of the TopTaq DNA Polymerase and Master Mix Kits interfere with downstream applications?
FAQ ID -1744
浏览
Do you have a forensic post-PCR purification protocol to purify double-stranded DNA fragments from PCR reactions?
FAQ ID -1761
浏览
Do CoralLoad dyes supplied in various QIAGEN PCR Kits interfere with downstream applications?
FAQ ID -1745
浏览
Is FlexiTube GeneSolution available for other scales than the 1 nmol scale?
FAQ ID -1666
浏览
What buffer should be used to dilute cDNA made using the miScript Reverse Transcription Kit?
FAQ ID -1601
浏览
What is the binding capacity of the Ni-NTA Superflow Cartridges?
FAQ ID -1603
浏览
Is it necessary to clean up cDNA prepared with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1610
浏览
Is the master mix of QuantiFast Kits for real-time PCR aliquoted into several tubes to prevent cross-contamination?
FAQ ID -1697
浏览
Is the QuantiTect Whole Transcriptome Kit suitable for miRNA amplification?
FAQ ID -1609
浏览
Is sequence information for HP Validated siRNAs provided in the FlexiTube format?
FAQ ID -1665
浏览
Is it necessary to include a genomic DNA removal step with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1614
浏览
Which siRNA controls are available in FlexiTube format?
FAQ ID -1661
浏览
Where can I find background information and literature on Whole Genome Amplification with REPLI-g Kits?
FAQ ID -1690
浏览
Why is there DNA in the no-template (negative) control when using the QuantiTect Whole Transcriptome Kit?
FAQ ID -1620
浏览
What is the composition of Buffer PE?
FAQ ID -1691
浏览
What is the maximum volume of RNA in solution that can be used with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1616
浏览
Can the Ni-NTA Superflow Cartridges and/or Strep-Tactin Cartridges be connected in series?
FAQ ID -1606
浏览
Can the Ni-NTA and Strep-Tactin Superflow Cartridges be reused?
FAQ ID -1607
浏览
Can I store cDNA generated by the miScript System?
FAQ ID -1602
浏览
Can the QuantiTect Whole Transcriptome Kit be used for amplification of RNA from LCM samples?
FAQ ID -1612
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Does the QuantiTect Whole Transcriptome Kit work with RNA purified from bacteria, yeast, or plants?
FAQ ID -1615
浏览
Can T-Script® enzyme of the QuantiTect Whole Transcriptome Kit be substituted by Quantiscript Reverse Transcriptase?
FAQ ID -1617
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Can the QuantiTect Whole Transcriptome reaction be stopped after the reverse transcription step to proceed with the ligation and amplification steps at a later time?
FAQ ID -1618
浏览
Are FlexiTube siRNA and FlexiTube GeneSolution also available for rat siRNAs?
FAQ ID -1664
浏览
Can I still reorder siRNA that has been removed from the GeneGlobe data base?
FAQ ID -1668
浏览
Can amplification with the QuantiTect Whole Transcriptome Kit be extended to more than 8 hours to improve cDNA yields?
FAQ ID -1608
浏览
Can FlexiTube siRNA or FlexiTube GeneSolution products be ordered in solution?
FAQ ID -1667
浏览
Can I select HP Validated and HP GenomeWide siRNAs for FlexiTube?
FAQ ID -1658
浏览
Can cDNA prepared with the QuantiTect Whole Transcriptome Kit be reampliied using REPLI-g Kits?
FAQ ID -1611
浏览
How comparable is CoralLoad gel loading dye contained in various QIAGEN PCR Kits to Sigma Red?
FAQ ID -1644
浏览
How fast is the 6xHis-tagged protein purification process using Ni-NTA Superflow Cartridges?
FAQ ID -1605
浏览
How long is cDNA generated with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1613
浏览
How long can I archive cDNA generated with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1623
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Have you observed co-amplification of genomic DNA from RNA templates used in the QuantiTect Whole Transcriptome Procedure?
FAQ ID -1619
浏览
Do I need to resuspend FlexiTube siRNAs in siRNA Resuspension buffer?
FAQ ID -1659
浏览
Do you have a protocol for purification of total RNA from plant latex?
FAQ ID -1694
浏览
Do I need to use the miScript Universal Primer for detection of mRNA?
FAQ ID -1600
浏览
Do you offer a QuantiFast Kit for one-step RT-PCR?
FAQ ID -1695
浏览
Do you have a protocol for purification of total RNA from plant tissue with the RNeasy Lipid Tissue Mini Kit?
FAQ ID -1693
浏览
How many samples can be processed with the QIAsymphony DNA Mini Kit and the Virus Blood 200 protocol?
FAQ ID -2159
浏览
How often can the Strep-Tactin Superflow Plus resin be reused?
FAQ ID -2169
浏览
If housekeeping and target genes differ significantly in abundance, will they both be amplified with equal efficiency using the Rotor-Gene Multiplex PCR Kit?
FAQ ID -2129
浏览
If Vapor-Lock is used to overlay PCR reactions, which volume should be entered as reaction volume in the Rotor-Gene Q Software?
FAQ ID -2150
浏览
Is it normal that the binding capacity of GST-resins decreases with flow rate?
FAQ ID -2174
浏览
Is it possible to purify a GST-tagged protein under denaturing conditions using Glutathione Superflow?
FAQ ID -2172
浏览
Why is EvaGreen instead of SYBR Green used as fluorescent dye in the Type-it HRM PCR Kit?
FAQ ID -2196
浏览
Why is the initial activation step different for Rotor-Gene Probe, SYBR Green and Multiplex Kits?
FAQ ID -2118
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Why is a 2-step (and not a 3-step) cycling protocol recommended for Rotor-Gene SYBR Green Kits?
FAQ ID -2122
浏览
Why do I need normalization using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2198
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Why is there an asterisk for my miScript Primer Assays?
FAQ ID -2176
浏览
Will Rotor-Gene Kits also work on the Rotor-Gene 6000 and 3000 cyclers?
FAQ ID -2121
浏览
Will Uracil-N-Glycosylase (UNG) completely remove contaminating amplicons when using QuantiTect +UNG Kits?
FAQ ID -2139
浏览
Will QuantiTect Primer Assays work with Rotor-Gene SYBR Green Kits using an annealing step at 60ºC?
FAQ ID -2124
浏览
What is miRNA*? Do I need to consider this in my profiling experiment?
FAQ ID -2179
浏览
What are the main differences between Rotor-Gene and QuantiTect or QuantiFast PCR Kits?
FAQ ID -2119
浏览
What are possible reasons for reduced DNA yields with REPLI-g Kits?
FAQ ID -2148
浏览
What is the residuals plot in the Rotor-Gene ScreenClust HRM Software?
FAQ ID -2199
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What is the target nucleic acid for the Virus Blood 200 Protocol on the QIAsymphony?
FAQ ID -2158
浏览
What is the minimum number of assays that can be ordered on QuantiTect Primer Assay Plates?
FAQ ID -2109
浏览
What is the detection limit of the Rotor-Gene and QuantiFast Multiplex RT-PCR Kits?
FAQ ID -2144
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What is the maximum number of targets that can be amplified per reaction with the QuantiFast Multiplex RT-PCR Kit?
FAQ ID -2145
浏览
How should DNA isolated on the QIAsymphony be stored?
FAQ ID -2165
浏览
How should samples be stored before purification using the Virus Blood 200 Protocol on the QIAsymphony?
FAQ ID -2157
浏览
How pure should the chloroform used with the RNeasy Microarray Tissue Mini Kit be?
FAQ ID -2193
浏览
Why should I use the RNeasy Microarray Tissue Mini Kit when purifying RNA for microarray analysis?
FAQ ID -2188
浏览
Which is the best condition for eluting GST-tagged proteins from Glutathione Superflow?
FAQ ID -2173
浏览
Which elution volumes can be selected using the QIAsymphony Virus Blood 200 protocol?
FAQ ID -2160
浏览
What sample volumes can be used with the Virus Blood 200 Protocol on the QIAsymphony?
FAQ ID -2156
浏览
Which plate formats are offered for QuantiTect Primer Assay Plates?
FAQ ID -2106
浏览
What species are QuantiTect Primer Assay Plates offered for?
FAQ ID -2110
浏览
Why are the denaturation and annealing/extension times for QuantiFast Multiplex RT-PCR Kits much shorter than those for QuantiFast Multiplex PCR Kits?
FAQ ID -2147
浏览
Which sample types can be processed using the QIAsymphony Virus Blood 200 protocol?
FAQ ID -2154
浏览
How many reactions can be performed per well of the QuantiTect Primer Assay Plates?
FAQ ID -2107
浏览
Can I use the RNeasy Microarray Tissue Mini Kit for all tissue types?
FAQ ID -2190
浏览
Can assays for 20 and 100 reactions be ordered on the same QuantiTect Primer Assay Plate?
FAQ ID -2108
浏览
Can Glutathione Superflow resin be reused?
FAQ ID -2171
浏览
Can Uracil-N-Glycosylase (UNG) be added to the QuantiTect PCR Master Mix and be stored for later use?
FAQ ID -2138
浏览
Can QuantiTect Primer Assay Plates be ordered offline via e-mail or fax?
FAQ ID -2112
浏览
Are there any changes in the procedure using the Strep-Tactin Superflow Plus resin with the higher capacity over the standard matrix?
FAQ ID -2168
浏览
Can Uracil-N-Glycosylase (UNG) be purchased separately, independent of QuantiTect PCR Kits?
FAQ ID -2134
浏览
Are probes other than TaqMan® probes, such as FRET probes, compatible with fast cycling with the Rotor-Gene Multiplex PCR Kit?
FAQ ID -2130
浏览
Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with the Rotor-Gene Multiplex PCR Kit?
FAQ ID -2131
浏览
Can the Rotor-Gene Multiplex RT-PCR Kit be used on other cyclers?
FAQ ID -2142
浏览
Can Uracil-N-Glycosylase (UNG) be used with a one-step RT-PCR kit, such as the QuantiTect Probe RT-PCR Kit?
FAQ ID -2135
浏览
Can RNA isolated with the RNeasy Microarray Tissue Mini Kit also be used in downstream applications other than microarray analysis?
FAQ ID -2191
浏览
Are Rotor-Gene Kits compatible with reaction setup using the QIAgility instrument?
FAQ ID -2116
浏览
Are QuantiTect PCR +UNG Kits available in bulk format (e.g., 25 ml master mix)?
FAQ ID -2136
浏览
Are the layouts for both 96- and 384-well QuantiTect Primer Assay Plates visible when ordering online?
FAQ ID -2111
浏览
Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with Rotor-Gene Probe Kits?
FAQ ID -2126
浏览
How does template quality influence the results with the Type-it HRM PCR Kit?
FAQ ID -2197
浏览
How does magnetic-particle carry-over in QIAsymphony eluates need to be handled?
FAQ ID -2166
浏览
Do you have protocols for software setup on various PCR platforms using QuantiFast Probe RT-PCR Kits?
FAQ ID -2105
浏览
How does the RNeasy Microarray Tissue Mini Kit prevent traces of phenol from negatively affecting the reverse-transcription step in microarray experiments?
FAQ ID -2189
浏览
How do Rotor-Gene Probe Kits compare with QuantiFast Probe Kits?
FAQ ID -2125
浏览
How many elution steps are recommended when using Glutathione Superflow resin?
FAQ ID -2175
浏览
How is the RNeasy Microarray Tissue Mini Kit different from the classic RNeasy Mini Kit?
FAQ ID -2192
浏览
Do you have a protocol for Rotor-Gene software setup for the Rotor-Gene SYBR Green PCR and RT-PCR Kits?
FAQ ID -2104
浏览
Does carrier RNA have to be added using the QIAsymphony Virus Blood 200 Protocol?
FAQ ID -2163
浏览
Does an internal control have to be used with the QIAsymphony Virus Blood 200 Protocol?
FAQ ID -2162
浏览
Can Vapor-Lock be used with samples processed on the QIAgility?
FAQ ID -2153
浏览
Do QuantiTect Primer Assays also work with Rotor-Gene SYBR Green PCR Kits?
FAQ ID -2123
浏览
Do the master mixes in Rotor-Gene Kits contain dUTP to allow UNG pretreatment?
FAQ ID -2117
浏览
Do limiting primer concentrations need to be determined when using the Rotor-Gene Multiplex PCR Kit?
FAQ ID -2128
浏览
What main input voltage is required for the QIAxcel Analyzer?
FAQ ID -1821
浏览
What is the minimum sample volume required for the QIAxcel System and QIAxcel Advanced?
FAQ ID -1852
浏览
Is there a quality difference between CompactPrep Plasmid Mega and Giga and the CompactPrep Plasmid Midi and Maxi Kits?
FAQ ID -1882
浏览
The Gel Cartridge is installed and all status indicators for the QIAxcel System are good but when clicking the "RUN" button, nothing happens. The green highlight goes down the Sequence?
FAQ ID -1850
浏览
Why does the copy function not apply relative to migration time and normalized area values after loading the DNA size marker table and adding the alignment marker peaks on the QIAxcel System?
FAQ ID -1832
浏览
What can be done if lower and upper alignment markers used with the QIAxcel System or QIAxcel Advanced are not aligned correctly?
FAQ ID -1834
浏览
Why are there no peaks in all channels during a run with the QIAxcel System or QIAxcel Advanced??
FAQ ID -1847
浏览
Why do DNA samples appear in only a few channels when the alignment marker is working using the QIAxcel System or QIAxcel Advanced?
FAQ ID -1844
浏览
Why does the Power LED not function with the QIAxcel or QIAxcel Advanced instrument plugged in and the main power switch turned on?
FAQ ID -1851
浏览
Can a QIAxcel DNA Fast Analysis Cartridge be run on a QIAxcel System?
FAQ ID -1828
浏览
Can customers use their own DNA size marker with the QIAxcel System and if yes, what concentration should be used?
FAQ ID -1841
浏览
Can data be saved at once with the QIAxcel System after analysis of all samples in a 96-well PCR plate?
FAQ ID -1861
浏览
How can a positive threshold be set when running the QIAxcel System?
FAQ ID -1843
浏览
How many intensity calibration runs can be performed per Gel Cartridge on the QIAxcel System?
FAQ ID -1827
浏览
How long will the intensity calibration procedure on the QIAxcel System take?
FAQ ID -1826
浏览
Why does an intensity calibration of the gel cartridge for the QIAxcel System and QIAxcel Advanced need to be performed?
FAQ ID -1824
浏览
Will the QIAxcel System run using a green Data Review Software Key?
FAQ ID -1853
浏览
Why is 'NO COM' displayed on the QIAxcel Instrument Panel although the power is on and all connections are made to the QIAxcel instrument and computer?
FAQ ID -1848
浏览
Why is the alignment marker for the 1.8 kb fragment not visible when using the 25 bp/1.8 kb DNA Size Marker together with the 15 bp/400 bp marker on the QIAxcel System and QIAxcel Advanced?
FAQ ID -1835
浏览
When running RNA samples using the QIAxcel System or QIAxcel Advanced, are there any special things to consider?
FAQ ID -1842
浏览
What to do if alignment marker and samples are migrating too slowly and the upper alignment marker does not appear on the gel- and/or electropherogram view with the QIAxcel System or QIAxcel Advanced?
FAQ ID -1833
浏览
Why are PCR bands relatively weak and "smearing" but the alignment marker bands are sharp when running samples on the QIAxcel System and QIAxcel Advanced?
FAQ ID -1837
浏览
When should an intensity calibration be re-performed on the QIAxcel System?
FAQ ID -1825
浏览
When should the N2 bottle used with the QIAxcel System be changed?
FAQ ID -1830
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Can the QIAxcel software be loaded to another PC to view the data generated with the QIAxcel System?
FAQ ID -1855
浏览
Can the QIAxcel System or QIAxcel Advanced be connected to a network?
FAQ ID -1862
浏览
How can a clogged capillary channel be identified when running samples on the QIAxcel System or QIAxcel Advanced?
FAQ ID -1836
浏览
Can multiple QIAxcel Systems be run from one computer?
FAQ ID -1860
浏览
If a different DNA size and/or alignment marker is to be used with the QIAxcel System or QIAxcel Advanced, does the Gel Cartridge need to be recalibrated?
FAQ ID -1858
浏览
If a different method is used with the QIAxcel System or QIAxcel Advanced, does a new reference marker table need to be created?
FAQ ID -1857
浏览
How often should the alignment marker for the QIAxcel System be changed?
FAQ ID -1839
浏览
How often should the solutions in the Buffer Tray for the QIAxcel System or QIAxcel Advanced be replaced?
FAQ ID -1840
浏览
In the results table generated with the QIAxcel System, what does "normalized area" mean?
FAQ ID -1854
浏览
How can drying out of the gel cartridge tips used on the QIAxcel System and QIAxcel Advanced be prevented?
FAQ ID -1823
浏览
If the M500 method is used with the QIAxcel System, does a new DNA reference marker table have to be created?
FAQ ID -1856
浏览
How can any clogged capillaries be cleared for use with the QIAxcel System and QIAxcel Advanced?
FAQ ID -1838
浏览
What is the composition of buffer QBT?
FAQ ID -411
浏览
What is the composition of buffer QC?
FAQ ID -412
浏览
What is the composition of buffer P3?
FAQ ID -418
浏览
What is the composition of buffer QF?
FAQ ID -413
浏览
What is the composition of buffer TE?
FAQ ID -416
浏览
What is the composition of buffer QN?
FAQ ID -414
浏览
What is the composition of buffer OCD?
FAQ ID -423
浏览
What is the composition of buffer FWB2?
FAQ ID -417
浏览
What is the composition of buffer OBB?
FAQ ID -421
浏览
What is the composition of buffer OW2?
FAQ ID -419
浏览
What is the composition of buffer OL2?
FAQ ID -424
浏览
What is the composition of buffer OCL?
FAQ ID -422
浏览
Can RNAi experiments be performed in Drosophila?
FAQ ID -436
浏览
What is the size, charge and isoelectric point of the DHFR protein in the pQE vectors?
FAQ ID -470
浏览
Can the QIAamp MinElute Virus Kits be used to extract viral nucleic acid from cell culture supernatant?
FAQ ID -472
浏览
Has RNAi been successful using siRNA in Zebrafish and Xenopus?
FAQ ID -400
浏览
How is the RNeasy Lipid Tissue Mini Kit different from the RNeasy Mini Kit?
FAQ ID -468
浏览
How do I calculate the percentage of silencing with real-time RT-PCR for siRNA?
FAQ ID -498
浏览
Are Northern Blots sensitive enough to detect siRNA-induced gene silencing?
FAQ ID -403
浏览
Can QIAquick Kits be used to clean up RNA samples?
FAQ ID -490
浏览
Are HEPES, MOPS and PIPES buffers compatible with QIAGEN's PhosphoProtein Purification kit?
FAQ ID -482
浏览
Can Ni-NTA resins be used to purify protein with an internal His-tag?
FAQ ID -496
浏览
What is the composition of buffer STE?
FAQ ID -415
浏览
Can I use the RNeasy MinElute Cleanup Kit to clean up my in vitro transcription reaction?
FAQ ID -429
浏览
What is the composition of buffer OEB?
FAQ ID -420
浏览
Why are the centrifugation speeds for the QIAamp DNA Mini kit at 6000 x g? Can I spin at full speed?
FAQ ID -474
浏览
Which kit should be used to extract RNA from adipose tissue, brain, and other fatty animal tissues?
FAQ ID -467
浏览
Why is the pQE DNA provided in QIAexpress Kits blue in color?
FAQ ID -487
浏览
Why is carrier RNA used during the isolation of gDNA from microdissected samples with the QIAamp DNA Micro Kit?
FAQ ID -473
浏览
What is the minimum elution volume when using the RNeasy MinElute Cleanup Kit?
FAQ ID -432
浏览
What is the minimum elution volume when using the RNeasy Micro Kit?
FAQ ID -428
浏览
What is the smallest sample size that can be used with RNeasy Mini Kits?
FAQ ID -485
浏览
What is the maximum amount of starting material that can be processed with the RNeasy Micro Kit?
FAQ ID -426
浏览
What detection reagents (reporter fluorophores) do you recommend for the LiquiChip System?
FAQ ID -480
浏览
What is a homogeneous LiquiChip assay?
FAQ ID -476
浏览
What are the excitation and emission wavelengths of the reporter and classification lasers of the LiquiChip Instrument?
FAQ ID -479
浏览
What is the binding capacity of the RNeasy Micro Kit column?
FAQ ID -427
浏览
What is the composition and size of the LiquiChip Beads?
FAQ ID -475
浏览
What is the binding capacity of the RNeasy MinElute Cleanup Kit column?
FAQ ID -430
浏览
Is dihydrofolate reductase (DHFR) immunogenic?
FAQ ID -471
浏览
What are some of the assays that are adaptable to the LiquiChip System?
FAQ ID -477
浏览
My QuantiTect Master Mix did not freeze at degrees. Is it still ok to use?
-20
浏览
Will REPLI-g work at high temperatures?
FAQ ID -656
浏览
What is the run time for the BioSprint 96 DNA Blood protocol on the BioSprint 96 instrument?
FAQ ID -622
浏览
The Multiflow Dispense Head on my BioRobot is not dispensing the correct amount of liquid. What can I do?
FAQ ID -652
浏览
What is the smallest amount of DNA I can put into a 50 ul REPLI-g reaction?
FAQ ID -663
浏览
What is the shelf-life of my QIAGEN Kit?
FAQ ID -651
浏览
What is the recommended incubation time to stabilize tissue RNA in RNAprotect?
FAQ ID -647
浏览
What do you mean by “Quantification of locus representation” in REPLI-g WGA product?
FAQ ID -698
浏览
What is the average amount of DNA and RNA present in 1 ml normal serum?
FAQ ID -635
浏览
What is REPLI-g whole genome amplification?
FAQ ID -654
浏览
What is a QIAshredder? Is it sufficient for complete disruption and homogenization of my tissue sample?
FAQ ID -631
浏览
What do you recommend for the cleanup of genomic DNA (gDNA)?
FAQ ID -618
浏览
What is the expected yield of genomic DNA isolated from bacteria with the DNeasy Blood & Tissue or QIAamp DNA Mini Kit?
FAQ ID -632
浏览
What is the biggest protein that can be synthesized with the EasyXpress Protein Synthesis System?
FAQ ID -601
浏览
What is the length of the MDA whole genome amplification product?
FAQ ID -690
浏览
What 96-well plates do you recommend for use with the LiquiChip Workstation?
FAQ ID -630
浏览
Is it possible to perform laser microdissection with RNAprotect-stabilized tissue?
FAQ ID -608
浏览
Is it possible to synthesize membrane proteins using the EasyXpress Protein Synthesis Kit?
FAQ ID -602
浏览
What are Elution Microtube adapters and Microplate adapters used in the DirectPrep 96 Miniprep Kit, and how can I order them?
FAQ ID -646
浏览
What are the differences between MDA and DOP/PEP methods of Whole Genome Amplification?
FAQ ID -665
浏览
What are the advantages of REPLI-g over conventional DNA sample processing and amplification methods?
FAQ ID -686
浏览
Why do I get amplification in a negative control DNA tube using the REPLI-g Kit for WGA?
FAQ ID -675
浏览
Why does the QIAamp DNA Mini Tissue Protocol require both ATL and AL buffer, while the Blood Protocol only uses AL?
FAQ ID -633
浏览
Why do I sometimes get light blue colonies when using the QIAGEN PCR Cloning Kit?
FAQ ID -603
浏览
Why did you choose MAPK1 as a positive control in the QIAGEN RNAi Human/Mouse Control Kit?
FAQ ID -604
浏览
Why do you recommend using Triton X for the purification of 6xHis-tagged protein?
-100
浏览
Will the PhosphoSerine antibody recognize Serine residues if the neighboring residues are Leucine or Isoleucine?
FAQ ID -644
浏览
Will the QIAquick PCR Purification Kit remove sufficient SYBR Green from real-time PCR reactions to allow sequencing?
FAQ ID -637
浏览
Will TA cloning work with REPLI-g WGA product?
FAQ ID -672
浏览
Will DNA from chromosomes collected in TE with a drop of mineral oil work with REPLI-g amplification?
FAQ ID -664
浏览
What is the stability of the REPLI-g MDA product?
FAQ ID -693
浏览
When delivering liquid to a 96-well plate using the MultiFlow Dispenser Head on my BioRobot , one or more rows in the plate contains less liquid per well than specified by the protocol. What is going wrong?
FAQ ID -653
浏览
Where should I add a dye-label modification on the siRNA?
FAQ ID -648
浏览
What types of materials from environmental samples could interfere with WGA?
FAQ ID -666
浏览
What size is the smallest protein that can be synthesized with the EasyXpress Protein Synthesis Kit?
FAQ ID -600
浏览
Can I stabilize RNA in bacteria grown on solid media or substrate using the RNAprotect Bacteria Reagent?
FAQ ID -615
浏览
Can I run less than 15 samples on the BioSprint 15 instrument?
FAQ ID -621
浏览
Can I re-amplify whole genome amplification product?
FAQ ID -670
浏览
Can I use another lysis buffer than the one supplied in the PhophoProtein Purification Kit?
FAQ ID -645
浏览
Can the QIAshredder Maxi Spin Columns from the DNeasy Plant Maxi Kit be used for RNeasy samples?
FAQ ID -616
浏览
Do you have an off-the shelf diagnostics kit for WGA?
FAQ ID -678
浏览
Can the RNase-Free DNase Set be used for DNase digestions of RNA in solution?
FAQ ID -619
浏览
Can the computer for the LiquiChip Workstation be networked?
FAQ ID -629
浏览
Are there components in biological samples that can inhibit the REPLI-g reaction?
FAQ ID -667
浏览
Are the BioSprint 15/96 instruments stand-alone workstations?
FAQ ID -620
浏览
Are samples that are contaminated with high molecular weight DNA (e.g. from a paramedic or police officer) a problem with REPLI-g amplification?
FAQ ID -683
浏览
Are QIAGEN EZ Competent Cells of the QIAGEN PCR Cloning Kit dam+ or dam-?
FAQ ID -638
浏览
Can BAC DNA prepared with the R.E.A.L. Prep 96 Plasmid Kit be used for high-throughput sequencing?
FAQ ID -643
浏览
Can I do whole genome amplification from brain tissue using REPLI-g?
FAQ ID -669
浏览
Can FTA paper for dried blood spots be used with REPLI-g?
FAQ ID -685
浏览
Can I do whole genome amplification from mitochondrial DNA using REPLI-g?
FAQ ID -668
浏览
Can I amplify from plasma or serum samples using the REPLI-g Mini/Midi kits?
FAQ ID -671
浏览
How can I minimize degradation of RNA from my pancreas sample?
FAQ ID -624
浏览
How can I quantify the amount of REPLI-g DNA I have amplified?
FAQ ID -694
浏览
How should I store the REPLI-g amplification product?
FAQ ID -692
浏览
Is 2 kb the minimal gDNA fragment size for REPLI-g Whole Genome Amplification?
FAQ ID -682
浏览
How long can I store bacterial culture stabilized in RNAprotect Bacteria Reagent?
FAQ ID -614
浏览
Do I need to clean up the REPLI-g amplification product for downstream analysis?
FAQ ID -691
浏览
Does REPLI-g technology for whole genome amplification work with paraffin embedded samples?
FAQ ID -673
浏览
Do you have a kit for RNA isolation from any kind of sample type?
FAQ ID -627
浏览
How can I improve my RNA yield from liver sample when using RNeasy Kits?
FAQ ID -623
浏览
How can I determine the quality of my REPLI-g amplified products?
FAQ ID -695
浏览
Do you know of anything in the buffers/DNA sample that can cause bias in REPLI-g WGA?
FAQ ID -684
浏览
What is the composition of the QIAGEN 10x PCR Buffer in Taq- and HotStarTaq DNA Polymerase Kits?
FAQ ID -606
浏览
Is it possible to use less than 1 mg of protein with the PhosphoProtein Purification Kit?
FAQ ID -599
浏览
I want to start with gene silencing (RNAi) experiments. Do you have informational literature?
FAQ ID -580
浏览
What can I do when the DNA pellet prepared with QIAGEN Plasmid Kits has been overdried?
FAQ ID -572
浏览
What is a quenched FRET assay?
FAQ ID -542
浏览
Is it possible to use a QIAzol lysate for the isolation of RNA from tissue on the BioRobot EZ1?
FAQ ID -573
浏览
What do you recommend for isolating RNA from more than 100 mg of plant tissue?
FAQ ID -570
浏览
What happens if I spin my lysate on the RNeasy Spin Columns at maximum speed?
FAQ ID -514
浏览
What has to be done to a RNA sample before loading it onto an Agilent Bioanalyzer?
FAQ ID -528
浏览
How should I dissolve the lyophilized Anti-His and Tag Antibodies?
-100
浏览
Is it possible to isolate single stranded DNA (ssDNA) with the QIAEX II Kit from agarose or polyacrylamide gels?
FAQ ID -576
浏览
Is it possible to perform a UNG treatment when using QuantiTect kits?
FAQ ID -564
浏览
Is it possible to isolate DNA from bone marrow with the QIAamp DNA Blood Kits?
FAQ ID -563
浏览
Is it possible to clean up a methylation reaction containing bisulfite with QIAquick Cleanup Kits?
FAQ ID -519
浏览
Is it possible to isolate both RNA and recombinant 6xHis-tagged protein from the same sample?
FAQ ID -532
浏览
Why are samples centrifuged at 4°C after the addition of chloroform when using RNeasy Lipid Tissue Kits?
FAQ ID -533
浏览
Why are my realtime PCR amplification plots hook-shaped?
FAQ ID -587
浏览
How was it determined that the PhosphoSerine Antibody will bind phosphoserines only?
FAQ ID -579
浏览
If pQE40 is used to express a fusion protein with DHFR, can the DHFR be cleaved afterwards?
FAQ ID -513
浏览
How should I store cDNA produced using Omniscript Reverse Transcriptase?
FAQ ID -561
浏览
Which chloroform should I use for the RNeasy Lipid Tissue Kit?
FAQ ID -516
浏览
Will pancreas tissue swim on top of RNAprotect?
FAQ ID -536
浏览
Will the 2x QIAGEN Multiplex PCR Master Mix freeze at -20°C?
FAQ ID - 525
浏览
Why does my realtime PCR assay quality decrease over time?
FAQ ID -589
浏览
Why do replicates in real-time PCR have different plateau heights?
FAQ ID -539
浏览
What is the longest fragment that was cloned with the pQE UA Cloning Vector?
-30
浏览
What is the difference between BSA-free and BSA-containing RGS-His Antibody?
FAQ ID -569
浏览
What is the composition of Buffer ER? Is it available separately?
FAQ ID -571
浏览
What is the composition of the siRNA resuspension & annealing buffer?
FAQ ID -522
浏览
When using QIAzol Lysis Reagent for RNA extraction, I see a pinkish colored aqueous phase! Is it OK?
FAQ ID -534
浏览
Where can I find the public Smith-Waterman homology search tool that you refer to on your siRNA online design page?
FAQ ID -562
浏览
What reaction volume should I use with QuantiTect Kits?
FAQ ID -551
浏览
What is Tissue-Tek O.C.T., and what is it used for?
FAQ ID -530
浏览
What makes QIAGEN's 10x Taq and HotStarTaq DNA Polymerase PCR buffer superior?
FAQ ID -566
浏览
How many template copies can I detect using QuantiTect Kits in real-time PCR?
FAQ ID -543
浏览
Can I use RNAiFect for co-transfection of plasmid DNA and siRNA?
FAQ ID -515
浏览
Does DEPC harm RNA?
FAQ ID -529
浏览
Does endogenously expressed DAPase in human cells degrade proteins that are expressed in cell culture?
FAQ ID -527
浏览
Can Taq DNA Polymerase use RNA as a template, and generate false positives in "no-RT" controls?
FAQ ID -523
浏览
Can the MAPK1 control siRNA be used for rat cells?
FAQ ID -547
浏览
Are the columns of the MinElute Reaction Cleanup-, Gel Extraction-, and PCR Purification Kit identical?
FAQ ID -581
浏览
Are the columns of the QIAquick PCR Purification-, Gel Extraction-, and Nucleotide Removal Kit interchangeable?
FAQ ID -577
浏览
After using your U/A PCR Cloning Kit I can detect colonies, which are light blue, and not really white. Why is that?
FAQ ID -512
浏览
Can I make a master mix with QuantiProbes, primers, and all other reaction components and store it for later use?
FAQ ID -584
浏览
Can I shorten the activation time for the HotStarTaq DNA Polymerase?
FAQ ID -565
浏览
Can I homogenize samples in QIAzol using the QIAshredder?
FAQ ID -501
浏览
Do you offer QIAshredder spin columns for the RNeasy Midi/Maxi Kit format? If not, what is the alternative?
FAQ ID -560
浏览
How can I avoid primer-dimer formation during PCR amplification?
FAQ ID -544
浏览
Does QIAGEN sell 6xHis-tagged proteins besides the 6xHis Protein Ladder?
FAQ ID -594
浏览
Do you provide control proteins for use with the PhosphoProtein Purification Kit?
FAQ ID -596
浏览
How long should the amplicon be if I am using QuantiTect SYBR Green detection?
FAQ ID -553
浏览
How is an antibody epitope determined?
FAQ ID -593
浏览
Do I need to calibrate my real-time cycler if I want to use Yakima Yellow?
FAQ ID -540
浏览
How can I tell if I have primer-dimers in my PCR reaction?
FAQ ID -552
浏览
Do we recommend pooling siRNA sequences?
FAQ ID -517
浏览
Do I have to remove the oil from my PCR reaction before using the QIAquick or MinElute PCR Purification Kit?
FAQ ID -575
浏览
Does the His-tag interfere with the purification procedure of the QIAGEN PhosphoProtein Purification Kit?
FAQ ID -598
浏览
Does the use of MAPK1 as a control in siRNA transfection experiments have secondary effects on the cell culture?
FAQ ID -548
浏览
Do you have a protocol to isolate RNA from blood and bone marrow using the BioRobot EZ1?
FAQ ID -567
浏览
Do you have stability data for genomic DNA isolated with the QIAamp DNA Blood Mini Kit?
FAQ ID -518
浏览
Do you have a protocol for the removal of endotoxins from already purified plasmid DNA?
FAQ ID -500
浏览
Do you have a protocol for isolating RNA from yeast using RNeasy?
FAQ ID -535
浏览
Do you have any recommendations for using the PosphoProtein Kit with starting material other than cells?
FAQ ID -597
浏览
Do you have a protocol for isolation of genomic DNA from saliva using the DNeasy Blood & Tissue Kit?
FAQ ID -931
浏览
Do you have a protocol for the isolation of DNA from epithelial cells mixed with sperm cells?
FAQ ID -926
浏览
Do you have a protocol for the purification of DNA from rodent tail using the BioSprint System?
FAQ ID -979
浏览
Do you have a protocol for the isolation of DNA from tofu?
FAQ ID -932
浏览
Do you have a protocol for Streptokinase treatment of clotted blood?
FAQ ID -912
浏览
Do you have a protocol for purification of DNA from clotted blood using the FlexiGene DNA Kit?
FAQ ID -973
浏览
Do you have a protocol for the isolation of RNA from leukocytes in milk?
FAQ ID -952
浏览
Do you have a protocol for the isolation of total nucleic acids from animal and human tissues on the BioRobot EZ1?
FAQ ID -975
浏览
Do you have a protocol for the isolation of total nucleic acids from animal and human cells on the BioRobot EZ1?
FAQ ID -974
浏览
Do you have a protocol for the isolation of total RNA from buccal swabs?
FAQ ID -956
浏览
Do you have a protocol for the isolation of viral DNA from stool?
FAQ ID -929
浏览
Do you have a protocol for the isolation of total RNA from plant tissue using QIAGEN-tips?
FAQ ID -949
浏览
Do you have a protocol for the isolation of total RNA from paraffin-embedded sections on the BioRobot EZ1?
FAQ ID -976
浏览
Do you have a protocol for the isolation of total RNA from plants using the RNeasy 96 Kit?
FAQ ID -951
浏览
Do you have a protocol for the isolation of DNA from soft tissues using the TissueLyser?
FAQ ID -924
浏览
Do you have a protocol for the isolation of genomic DNA from sperm?
FAQ ID -909
浏览
Do you have a protocol for the isolation of DNA from formalin-preserved stool samples?
FAQ ID -922
浏览
Do you have a protocol for the isolation of DNA from fungi using the BioRobot EZ1?
FAQ ID -992
浏览
Do you have a protocol for the isolation of genomic DNA from fungi?
FAQ ID -911
浏览
Do you have a protocol for the isolation of genomic DNA and/or proteins from fatty tissue treated with QIAzol?
FAQ ID -955
浏览
Do you have a protocol for the isolation of genomic DNA from frozen clotted blood?
FAQ ID -900. Test
浏览
Do you have a protocol for the preparation of RNAprotect preserved tissues for pathological studies?
FAQ ID -950
浏览
Do you have a protocol for the purification of DNA from cells using the BioSprint System?
FAQ ID -977
浏览
Do you have a protocol for the purification of DNA from dried blood spots using the BioSprint System?
FAQ ID -982
浏览
Do you have a protocol for isolation of genomic and plasmid DNA from cell cultures using QIAGEN Genomic-tips?
FAQ ID -902
浏览
Do you have a protocol for transfection of adherent cells with low concentrations of siRNA using RNAiFect Transfection Reagent?
FAQ ID -970
浏览
Do you have a protocol for the purification of DNA from buccal swabs using the BioSprint System?
FAQ ID -983
浏览
Do you have a protocol for GMO testing of food samples?
FAQ ID -921
浏览
Do you have a protocol for isolation of bacterial DNA from soil?
FAQ ID -923
浏览
Do you have a protocol for isolation of genomic and viral DNA from lymphocytes using QIAGEN Genomic-tips?
FAQ ID -901
浏览
Do you have a protocol for isolation of genomic DNA from saliva and mouthwash?
FAQ ID -917
浏览
Do you have a protocol for isolation of genomic DNA from dried blood spots using the QIAamp 96 DNA Blood Kit?
FAQ ID -919
浏览
Do you have a protocol for isolation of genomic DNA from insects?
FAQ ID -904
浏览
Do you have a protocol for detection of HBV DNA by PCR?
FAQ ID -915
浏览
Can the BioSprint instruments be placed in a cool room?
FAQ ID -938
浏览
Does QIAGEN sell a vector with a T7 promotor for use with the EasyXpress Protein Synthesis Insect Kit?
FAQ ID -937
浏览
Do you have a protocol for Acetyl Cysteine (NALC) treatment of viscous samples?
FAQ ID -913
浏览
Do you have a protocol for co-transfection of adherent cells with siRNA and plasmid DNA?
FAQ ID -969
浏览
Do you have a protocol for Cyanine 570, Cyanine 670, or biotin labeling of cDNA?
FAQ ID -959
浏览
Do you have a protocol for adding new reporter dyes to the ABI PRISM 7900 Sequence Detection System?
FAQ ID -965
浏览
Do you have a protocol for cleanup of non-radioactive DNA samples?
FAQ ID -945
浏览
Do you have a protocol for radioactive labelling of cDNA?
FAQ ID -960
浏览
Do you have a protocol for reverse transfection of adherent cells with siRNA in 384-well plates?
FAQ ID -971
浏览
Do you have a protocol for the isolation of genomic DNA from bone?
FAQ ID -908
浏览
Do you have a protocol for the isolation of viral RNA from stool?
FAQ ID -918
浏览
Do you have protocols for the isolation of genomic DNA from tissue using QIAGEN-tips 2500 (Mega) and 10000 (Giga)?
FAQ ID -907
浏览
Do you have protocols for multiple extractions of DNA fragments from agarose gels?
FAQ ID -944
浏览
Why is it not recommended to stabilize cells with RNAprotect?
FAQ ID -941
浏览
What do you suggest to prevent degradation of RNA isolated from tissue with high amounts of RNases using RNeasy?
FAQ ID -942
浏览
Do you have a protocol for transient transfection of Hela-S3 cells using PolyFect Transfection Reagent?
FAQ ID -997
浏览
Do you have a protocol for the detection of Bordetella pertussis DNA by PCR?
FAQ ID -914
浏览
Do you have a protocol for the isolation of DNA from buffy coat using the BioRobot EZ1?
FAQ ID -985
浏览
Do you have a protocol for the isolation of DNA from cultured cells using the BioRobot EZ1?
FAQ ID -990
浏览
Do you have a protocol for performing quantitative multiplex PCR on the LightCycler 2.0 system?
FAQ ID -966
浏览
Do you have a protocol for long-term, siRNA-mediated gene silencing using HiPerFect Transfection Reagent?
FAQ ID -972
浏览
Do you have a protocol for polyacrylamide gel analysis of oligonucleotides?
FAQ ID -961
浏览
Do you have a protocol for manual purification of 6xHis-tagged proteins expressed in E. coli using Ni-NTA Superflow?
FAQ ID -967
浏览
Do you have a protocol for purification of DNA from cultured animal cells using the DNeasy 96 Blood & Tissue Kit?
FAQ ID -934
浏览
Do you have a protocol for purification of DNA fragments from Cy3/Cy5 dye-label reactions?
FAQ ID -947
浏览
Do you have a protocol for purifying 2x96 PCR samples simultaneously with the QIAquick 96 PCR Purification Kit?
FAQ ID -935
浏览
Do you have a protocol for the purification of PCR products using the BioSprint System?
FAQ ID -984
浏览
Do you have a protocol for isolation of genomic DNA from plants and filamentous fungi using QIAGEN Genomic-tips?
FAQ ID -905
浏览
Do you have a protocol for isolation of genomic DNA from flies using QIAGEN Genomic-tips?
FAQ ID -903
浏览
Is it possible to transfect DNA into insect cell lines such as Drosophila melanogaster S2?
FAQ ID -397
浏览
Is it possible to isolate free genomic DNA using the QIAamp UltraSens Virus Kit?
FAQ ID -358
浏览
Is QIAGEN carrier RNA sold separately?
FAQ ID -351
浏览
I accidentally added RNAprotect to my cells, and now the cells are difficult to pellet. What can I do?
FAQ ID -364
浏览
What are the calculated molecular weights of the TAGZyme enzymes?
FAQ ID -329
浏览
Is it possible to store TAGzyme enzymes at°C?
-80
浏览
Is Q-Solution required for PCR with QIAGEN's PCR kits?
FAQ ID -380
浏览
How do I know if my plasmid is a high- or low copy number type?
FAQ ID -350
浏览
How do I perform a DNA precipitation to concentrate my sample?
FAQ ID -305
浏览
How can QIAGEN Protease and Proteinase K be inactivated?
FAQ ID -315
浏览
How complete is the removal of DAPase in the TAGZyme process?
FAQ ID -319
浏览
How long is fluorescence detectable in cells after transfection with fluorescently labeled siRNAs?
FAQ ID -392
浏览
How many transfections can I perform with 20 nmol of HPP grade siRNA?
FAQ ID -367
浏览
How do I submit a siRNA order by telephone or online?
FAQ ID -399
浏览
What is the white insoluble precipitate in my resuspended plasmid DNA pellet?
FAQ ID -352
浏览
What kits does QIAGEN offer to extract RNA from whole blood?
FAQ ID -304
浏览
Why do I get genomic DNA contamination in my plasmid prep?
FAQ ID -353
浏览
Which primers can I use for sequencing pQE-expression vector constructs?
FAQ ID -343
浏览
What is the standard assay volume recommended for LiquiChip assays?
FAQ ID -330
浏览
What is the average molecular weight of a siRNA, and how do I convert uM to ug values?
FAQ ID -388
浏览
What are the dissociation constants for the Anti-His Antibodies?
FAQ ID -385
浏览
What is the lowest elution volume that can be used with QIAprecipitator Midi and Maxi Modules?
FAQ ID -307
浏览
What is the origin of replication and the plasmid copy number of the pQE vectors?
FAQ ID -338
浏览
What is the acceptable maximum g force for centrifugation of Xa Removal Resin?
FAQ ID -320
浏览
What is the composition of Buffer FG3?
FAQ ID -356
浏览
What is the composition of PBS?
FAQ ID -361
浏览
How can I keep my centrifuge tubes endotoxin-free?
FAQ ID -301
浏览
Can I use ethanol instead of isopropanol for DNA precipitation when using HiSpeed Plasmid Kits?
FAQ ID -354
浏览
303 - Can I use my own lysis buffer with the DNeasy Blood & Tissue or QIAamp DNA Mini Kit?
浏览
Can I store agarose gel slices containing DNA for gel extraction at a later point?
FAQ ID -313
浏览
Can I use a partial MinElute 96 UF plate?
FAQ ID -355
浏览
Can I use the RNeasy Mini Kit or RNeasy 96 Kit with fewer than 100 cells?
FAQ ID -372
浏览
Can I use water for elution from the QIAprecipitator of the HiSpeed Plasmid Kits?
FAQ ID -306
浏览
Are QIAprep and QIAquick Spin columns interchangeable?
FAQ ID -311
浏览
Are the QIAprecipitator Midi and Maxi Modules of the HiSpeed Plasmid Kits interchangeable?
FAQ ID -308
浏览
Apart from the antibody itself, does the Penta-His Biotin Conjugate solution contain any protein?
FAQ ID -359
浏览
Are QIAGEN monoclonal Anti His Antibodies glycosylated?
FAQ ID -334
浏览
Can I eliminate RNase A from buffer P1 for my plasmid preparation to obtain RNase-free DNA for in-vitro transcription?
FAQ ID -366
浏览
Can I perform in-vitro transcription with the pDrive Cloning Vector from the QIAGEN PCR Cloning Kit?
FAQ ID -332
浏览
Can Buffers N3 and P3 be used interchangeably?
FAQ ID -310
浏览
Do the QuantiTect SYBR Green Kits contain a stabilization reagent that inhibits the sensitivity of real-time PCR?
FAQ ID -328
浏览
How are siRNAs introduced into C. elegans?
FAQ ID -396
浏览
Do I need to anneal, deprotect or desalt my QIAGEN siRNA?
FAQ ID -398
浏览
Do pQE vectors contain the CAT gene?
FAQ ID -362
浏览
How can I improve DNA yields from very tough tissues using the DNeasy Blood & Tissue Kit or the QIAamp DNA Mini Kit?
FAQ ID -374
浏览
How can I improve the expression of proteins containing hydrophobic regions?
FAQ ID -339
浏览
How can I check if any residual proteins remain on the Ni-NTA Agarose matrix after elution?
FAQ ID -324
浏览
How can I express toxic protein in E. coli?
FAQ ID -373
浏览
Do freeze-thaw cycles affect the stability of the U-overhang on the pDrive Cloning Vector?
FAQ ID -323
浏览
Can RNAprotect Tissue Reagent be used to store previously isolated RNA?
FAQ ID -337
浏览
Can MinElute 96 UF PCR be used to purify PCR samples which contain mineral oil?
FAQ ID -360
浏览
Can siRNA silence bacterial genes?
FAQ ID -395
浏览
Does SYBR Green dye present in QuantiTect kits interfere with ethidium bromide staining?
FAQ ID -312
浏览
Does the 5x OneStep RT-PCR Buffer contain BSA?
FAQ ID -326
浏览
Do any of the buffers in the HotStarTaq DNA Polymerase Kit contain Triton?
FAQ ID -327
浏览
Do any of the kit components or the product packaging of the QIAamp 96 DNA Blood Kit contain latex?
FAQ ID -318
浏览
What are exo-resistant random hexamers used in the REPLI-g reaction?
FAQ ID -710
浏览
The Sigma Centrifuge makes a loud noise at low speeds which disappears at higher speeds, when using the 2x96-well plate rotor. What is the problem?
FAQ ID -776
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Is RNAprotect Bacteria Reagent compatible with the RNeasy Mini Kit?
FAQ ID -797
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What is the average DNA concentration obtained using the DirectPrep 96 Miniprep Kit?
FAQ ID -721
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What are the expected average DNA yields from plant extractions on the BioSprint 96?
FAQ ID -771
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What is the basic technology behind the Strep-tag Protein Purification System?
FAQ ID -740
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What is the advantage of running an analytical gel with fractions of my plasmid preparation?
FAQ ID -769
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What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?
FAQ ID -728
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I bound an 11 kb DNA fragment to a QIAquick column; is it completely lost?
FAQ ID -756
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If template abundance differs significantly between housekeeping and target gene, are both amplified equally efficient with the QuantiTect Multiplex Kits?
FAQ ID -718
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How to quantitate DNA purified using the BioSprint 96?
FAQ ID -773
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How to store DNA from forensic samples eluted into water on the EZ1 biorobot in TE buffer instead?
FAQ ID -734
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I accidentally stored Buffer RDD of the RNase-Free DNase Set at°C. Will it still function?
-20
浏览
Is it possible to use LiquiChip beads for FACS applications?
FAQ ID -733
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Is it possible to use the QuantiTect Reverse Transcription Kit with bacterial RNA?
FAQ ID -785
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I ran my RNA out on an agarose gel and can see lots of bands similar to a ladder. Why?
FAQ ID -745
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Is GFP excited and detected by the green laser of the LiquiChip System?
FAQ ID -737
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Is it possible to buy the EZ Competent Cells of the QIAGEN PCR Cloning Plus Kit separately?
FAQ ID -763
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Which Qproteome or Protein Fractionation Kits from QIAGEN are compatible with tissue samples?
FAQ ID -755
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Why are some of my RNAprotect-stabilized samples frozen atºC while others are not?
ID -758
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Where can I find info on compatible reporter dyes for use in real-time multiplex PCR using the QuantiTect Multiplex PCR Kits?
FAQ ID -719
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Why are the QuantiTect and QuantiFast Multiplex PCR Kits limited to triplex real-time PCR on some cyclers?
FAQ ID -715
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Why might my gene of interest drop out during WGA if it is not near a telomere or centromere?
FAQ ID -703
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Will the random hexamers in the REPLI-g reaction interfere with downstream analysis?
FAQ ID -713
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Why should DNA or cDNA targets be less than 250 bp long for real-time PCR?
FAQ ID-751
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Why is my plasmid DNA yield low?
FAQ ID -768
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What is the difference between Ni-NTA Agarose and Ni-NTA Superflow?
FAQ ID -764
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What is the difference between QIAGEN Protease and QIAGEN Proteinase K provided in various QIAamp Kits?
FAQ ID -761
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Why is there no detection of free reporter fluorescence in the LiquiChip System?
FAQ ID -732
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What is the composition of buffer AE?
FAQ ID -730
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What is the cell number range one can use with the FastLane Cell cDNA Kit for real-time RT-PCR?
FAQ ID -782
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What is the composition of Buffer N3?
FAQ ID -767
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What negative (unphosphorylated) and positive (phosphorylated) control proteins do you recommend for use with the PhosphoProtein Purification Kit?
FAQ ID -779
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Where can I find a copy of the report files generated by running accessory protocols "Dilutor Delivery (monthly)" and "High-Speed Pipetting Sytem (monthly)" on my BioRobot 9604?
FAQ ID -766
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What type of Sequencing Services does QIAGEN offer?
FAQ ID -781
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What is the enzyme used in the REPLI-g reaction?
FAQ ID -704
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What is the recipe for SOC medium?
FAQ ID -798
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What is the general sensitivity level of the LiquiChip System?
FAQ ID -731
浏览
How should fluorescent labeled probes be stored?
FAQ ID -784
浏览
Can QIAGEN's Taq- and HotstarTaq DNA Polymerases be used for cycle sequencing?
FAQ ID -741
浏览
Can RNAprotect Tissue Reagent be used to stabilize tissue which has already been flash-frozen?
FAQ ID -757
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Can I use the PhosphoProtein Purification Kit to purify phosphorylated peptides of around 1 kD size?
FAQ ID -778
浏览
Can lyophilized plant material be used for DNA extraction on the BioSprint 96?
FAQ ID -772
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Can I use the QuantiTect Multiplex PCR Kits on the Roche LightCycler systems with TaqMan® probes or QuantiTect Assays?
FAQ ID -717
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Can the REPLI-g WGA enzyme work with labeled dNTPs?
FAQ ID -706
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Can the MAPK1 siRNA of the RNAi Human/Mouse Starter Kit be purchased separately?
FAQ ID -744
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Can the QIAprep Spin Miniprep Kit be used for isolating plasmid DNA from mammalian cells?
FAQ ID -795
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Can the QIAvac 96 vacuum manifold be used with DirectPrep 96 plates?
FAQ ID -722
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Are the buffers in the Ni-NTA Fast Start Kit the same as the ones for use with Ni-NTA purchased separately?
FAQ ID -791
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Are the protocols from the BioSprint software compatible with the KingFisher software and vice versa?
FAQ ID -770
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Are Buffer PB of the QIAquick PCR Purification Kit and Buffer QG of the QIAquick Gel Extraction Kit interchangeable?
FAQ ID -786
浏览
Any data on the fidelity of the REPLI-g MDA technique?
FAQ ID -707
浏览
Are Centromeres and Telomeres amplified using REPLI-g WGA?
FAQ ID -702
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Can I use my own primers for REPLI-g WGA to amplify a specific chromosomal region?
FAQ ID -712
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Can I use REPLI-g for SNP Genotyping?
FAQ ID -700
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Can I do 4-plex real-time PCR, on the ABI PRISM 7000, 7700, or 7900 using the QuantiTect Multiplex PCR Kit?
FAQ ID -716
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Can I purchase Phi29 DNA polymerase only?
FAQ ID -708
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How can I get specific information and a quote for my individual Sequencing Service needs?
FAQ ID -792
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How can one perform multiplex real-time PCR analysis using the LightCycler 2.0?
FAQ ID -780
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How can I be sure that I am harvesting my induced bacterial culture at the best time point for protein expression?
FAQ ID -788
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How can I get a certificate for quality assurance (ISO 13485 and ISO 9001) from QIAGEN?
FAQ ID -762
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How do the FastLane Cell cDNA Kit and QuantiTect Reverse Transcription Kit eliminate genomic DNA contamination?
FAQ ID -783
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How many cells can be used with the FastLane Cell cDNA Kit?
FAQ ID -796
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Do you have an antibody for Western Blot Analysis of MAPK1 gene knockdown using your MAPK1 control siRNA?
FAQ ID -746
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Do I have to switch off the laser of the LiquiChip Workstation overnight?
FAQ ID -736
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Can frozen blood be used for whole genome amplification with REPLI-g?
FAQ ID -794
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How many reporter dyes can be used in the same analysis using the LiquiChip Workstation?
FAQ ID -738
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How much DNA is obtained in the average PCR reaction?
FAQ ID -750
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Do I need to determine limiting primer concentrations with the QuantiTect Multiplex PCR Kits?
FAQ ID -714
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Do I need to have EDTA in the buffer in which I am going to store my isolated genomic DNA?
FAQ ID -754
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Do you synthesize thiophosphate modified random hexamers?
FAQ ID -711
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Has anyone verified whole genome amplification accuracy with Sequencing?
FAQ ID -701
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How best to handle the light sensitive LiquiChip reagents?
FAQ ID -735
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Do you have a protocol for the fixation of cells transfected with fluorescently labeled siRNA?
FAQ ID -793
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Do you have information about the cleanup of single-stranded DNA (ssDNA) with QIAquick columns?
FAQ ID -759
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Do you have information on the use of recombinant DNA and RNA as absolute standards for realtime RT-PCR?
FAQ ID -729
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Do you have a protocol for the isolation of genomic DNA from paraffin-embedded samples on the BioRobot M48?
FAQ ID -1019
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Is the TurboCapture Microplate Holder Type B compatible with the Biomek FX robotic workstation?
FAQ ID -1040
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How can I prevent running out of M48 Kit reagents before the full number of samples are processed?
FAQ ID -1042
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What are the additional plasmid bands I see on my gel?
FAQ ID -1059
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Is it possible to scale up QuantiTect Reverse Transcription reactions to allow use of larger amounts of RNA?
FAQ ID -1063
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Is it possible to use the PhosphoProtein Purification Kit in batch format?
FAQ ID -1069
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What are the recommended storage conditions for the QuantiTect Reverse Transcription Kit and its components?
FAQ ID -1077
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What is the difference between Buffers RLT and RLT Plus?
FAQ ID -1043
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What is the difference between TurboCapture Microplate Holders Type A and Type B?
FAQ ID -1038
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Is there a limit to the size of proteins that can be purified using the Qproteome Glycoprotein Kits?
FAQ ID -1071
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What blocking reagents do you recommend for chemiluminescent detection using Penta-, Tetra-, and RGS-His antibodies?
FAQ ID -1093
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Is Factor SB of the HotStar HiFidelity Polymerase Kit the same as Factor MP of the QIAGEN Multiplex PCR Kit?
FAQ ID -1049
浏览
How can I transfect siRNA into insect cells such as Drosophila melanogaster S2?
FAQ ID -1046
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How can I tell TurboCapture Microplate Holder Type A from Type B?
FAQ ID -1039
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How much template can I use in the reaction and what is the maximum volume of template that can be used in the QuantiTect Kits?
FAQ ID -1086
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I am seeing a precipitate after adding LyseBlue reagent to Buffer P1. What should I do about that?
FAQ ID -1045
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How much dye-labeled siRNA should I use to monitor transfection efficiency when using HiPerfect Transfection Reagent?
FAQ ID -1058
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What is the error rate of HotStar HiFidelity DNA Polymerase?
FAQ ID -1048
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How do I perform an Acetone Precipitation for concentrating and desalting protein samples?
FAQ ID -1035
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Which kit should I use for RNA isolation from Cartilage?
FAQ ID -1026
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Which QIAGEN kit do you recommend for purifying plasmid DNA suitable for transfection of sensitive cells?
FAQ ID -1092
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Where can I find the serial number on QIAGEN's BioRobots?
FAQ ID -1064
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Which kit can be used for simultaneous purification of DNA and RNA from biopsies?
FAQ ID -1089
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Why does my PCR product show up later when comparing the QuantiTect SYBR Green PCR Kits with Roche kits using the same annealing temperature?
FAQ ID -1083
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Why is there RNA in the DNA eluate when using the AllPrep DNA Mini Spin Column?
FAQ ID -1044
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Will the "classic" RNeasy Mini Kit be discontinued after the launch of the Allprep DNA/RNA Mini Kit?
FAQ ID -1054
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Why does TA/UA cloning work with your proofreading HotStar HiFidelity DNA Polymerase?
FAQ ID -1053
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Why do melting temperatures differ between PCR fragments amplified with QIAGEN's QuantiTect SYBR Green PCR Kits and Roche Kits?
FAQ ID -1084
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Where can I find a protocol for cleanup of already purified plasmid DNA?
FAQ ID -1031
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What is the protein loading capacity per column of the Qproteome Albumin/IgG Depletion Kit?
FAQ ID -1090
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What is the largest PCR amplicon that can be amplified with the HotStar HiFidelity Polymerase Kit?
FAQ ID -1047
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What is the range of melting temperatures for the QuantiTect Primer Assay amplicons in the dissociation curve?
FAQ ID -1079
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What is the recipe for 1x PBS solution?
FAQ ID -1030
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What should I use as a standard for absolute quantification in real-time PCR?
FAQ ID -1085
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What is your recommendation for cleaning the TissueLyser Bead Dispenser (96-well format)?
FAQ ID -1055
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What is the resin in the Qproteome Murine Albumin and Qproteome Albumin/IgG Depletion Kits?
FAQ ID -1034
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What is the volume of the eluate when using either spin or vacuum procedures with the QIAprep 96 Turbo Miniprep Kit?
FAQ ID -1067
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How can I prevent clogging of QIAfilter cartridges?
FAQ ID -1060
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Do I have to discard Buffer RLT with beta-Mercaptoethanol (ß-ME) added to it after 1 month of storage?
FAQ ID -1037
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Do I have to use a ramp time of 2°C/sec on the LightCycler when using Quantitect Primer Assays?
FAQ ID -1065
浏览
Does an activation time of 15 minutes influence the performance of the HotStarTaq Plus DNA Polymerase?
FAQ ID -1050
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Do I have to use CoralLoad Gel loading dye when using your HotStarTaq Plus DNA Polymerase?
FAQ ID -1052
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Do you have a protocol for the isolation of RNA from bacterial cultures using RNAprotect Bacteria Reagent and QIAzol Lysis Reagent?
FAQ ID -1020
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Do you have a protocol for the isolation of DNA from forensic samples using the BioRobot M48?
FAQ ID -1017
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Can the USB Port of the BioSprint 96 instrument be used to transfer programs from a PC to the instrument?
FAQ ID -1032
浏览
Do you have a protocol for the isolation of DNA from mouse tails using MagAttract technology on BioRobot M96?
FAQ ID -1007
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Are your FlexiTube siRNAs preferentially designed to any particular region of the target gene?
FAQ ID -1091
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Are the CoralLoad dyes in the HotStarTaq Plus PCR Buffer visible when loading small amounts of PCR product onto a gel?
FAQ ID -1051
浏览
Are the new EZ1 RNA Kits (cat. no. 958034; 959034; 956034) compatible with the previous EZ1 RNA Card (cat. no. 9015590) or EZ1 RNA Universal Tissue Card (cat. no 9016384)?
FAQ ID -1025
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Can PCR fragments generated with the EasyXpress Linear Template Kit be cloned into the pIX4.0 vector of the EasyXpress Protein Synthesis Insect Kit?
FAQ ID -1078
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Can the QProteome O-Glycan Glycoprotein Kits be used for blood cells?
FAQ ID -1036
浏览
Can the Reverse Transcriptases of the QuantiTect Reverse Transcription Kit and the QuantiTect Probe RT-PCR Kit be used interchangeably?
FAQ ID -1066
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Can the AllPrep RNA/Protein Kit be used with tissue?
FAQ ID -1095
浏览
Can the Blocking Reagent supplied with the Penta-His HRP Conjugate be used with other antibodies?
FAQ ID -1073
浏览
Do you recommend 1-step or 2-step real-time RT-PCR for gene expression analysis?
FAQ ID -1056
浏览
How can I check for purity of RNA isolated using RNeasy Kits?
FAQ ID -1023
浏览
How can air bubbles in PhosphoProtein Purification Columns be avoided?
FAQ ID -1070
浏览
How can I increase low-copy plasmid DNA yields using QIAfilter Plasmid Kits?
FAQ ID -1062
浏览
How can I increase DNA concentration using QIAprecipitators of the HiSpeed Plasmid Kits?
FAQ ID -1061
浏览
How can I check the integrity of RNA purified using RNeasy Kits?
FAQ ID -1024
浏览
How can I ensure complete genomic DNA removal when using the RNase-Free DNase Set?
FAQ ID -1087
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Do you have information on the reproducibility of DNA yields from blood using the M48 BioRobot?
FAQ ID - 1004
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Do you have a protocol for transient transfection of HeLa-S3 cells in 60 mm dishes using PolyFect?
FAQ ID -1012
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Do you have a protocol for transient transfection of HEK 293 cells in 75 cm3 flasks?
FAQ ID -1014
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Do you have a protocol for transient transfection of cells in 96-well plates using PolyFect Transfection Reagent?
FAQ ID -1013
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Do you have a protocol for the isolation of total nucleic acids from cells on the M48 BioRobot?
FAQ ID -1009
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Do you have a protocol for the isolation of total nucleic acids from tissue on the M48 BioRobot?
FAQ ID -1008
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Do you have a protocol for vacuum processing of blood samples with the QIAamp DNA Blood Midi or Maxi Kits?
FAQ ID -1011
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Do you have information on realtime RT-PCR performance of RNA isolated from monocytes on the M48 BioRobot?
FAQ ID -1010
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Do you have information on PCR template preparation from preserved blood samples on the M48 BioRobot?
FAQ ID - 1003
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Do you have information on DNA yields from blood with variable numbers of white blood cells using the M48 BioRobot?
FAQ ID -1006
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What Disinfectant Solution do you recommend for the BioRobot MDx?
FAQ ID -809
浏览
What data documentation does QIAGEN's Sequencing Service provide for customers?
FAQ ID -832
浏览
What are the sequences of the FlexiTube siRNAs?
FAQ ID -851
浏览
What annealing temperature should be used with the QuantiTect Primer Assays?
FAQ ID -849
浏览
What is the proper way to turn on or turn off the EZ1 instrument?
FAQ ID -870
浏览
My BioRobot 9604 came with several Accessory Applications. Is it recommended I run these protocols?
FAQ ID -872
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What is the Energy Mix in the EasyXpress Kits?
FAQ ID -846
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What is the concentration of the primers in a reaction using the QuantiTect Primer Assays?
FAQ ID -850
浏览
I would like to sequence my siRNA constructs. Does QIAGEN offer Sequencing Services for cloned siRNA?
FAQ ID -829
浏览
Is it possible to perform immunoprecipitation (IP) directly on protein fractions from the Qproteome Cell Compartment Kit?
FAQ ID -855
浏览
Is it possible to use frozen cell pellets with the FastLane Cell cDNA Kit?
FAQ ID -835
浏览
Is it possible to elute plasmid DNA from the QIAprep Spin Miniprep columns with buffer containing Potassium Phosphate?
FAQ ID -854
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Is it possible to adjust the Lysis Buffer volume used with the Qproteome Bacterial Protein Preparation Kit?
FAQ ID -841
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Is it possible to use QIAGEN's pQE-TriSystem Vectors with the EasyXpress Protein Synthesis Insect Kit?
FAQ ID -876
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Is there a stopping point in the protocol for Suspended Cells using the FastLane Cell cDNA Kit?
FAQ ID -837
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Is the random biotin labeling of proteins expressed with your EasyXpress Biotin Labeling Kit as sensitive as labeling with radioactivity?
FAQ ID -875
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Is the E. coli Host Strain M15[pREP4] resistant to Zeocin?
FAQ ID -842
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Will acetone precipitation recommended in the Qproteome Protocols denature Protein?
FAQ ID -807
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Will biotin detection assays be efficient when doing in vitro translation from E. coli lysates with the EasyXpress Site-Specific Biotin Kit?
FAQ ID -878
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Why would clumps occur following the addition of Buffer P2 when using LyseBlue Reagent in a plasmid preparation?
FAQ ID -862
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Why is it recommended to add 1 mM IPTG for optimal protein yields using the EasyXpress Protein Synthesis Kits?
FAQ ID -860
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With LyseBlue reagent for lysis control, can I now process more bacterial culture and overload the columns?
FAQ ID -864
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Will site-specific biotin labeling with the EasyXpress Site-Specific Biotin kit alter the structure and function of my protein?
FAQ ID -877
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Will the sequences of the QuantiTect Primer Assays be provided?
FAQ ID -804
浏览
Which resin is used in the QIAexpress Ni-NTA Fast Start Columns?
FAQ ID -836
浏览
What to do if cell clumps are present after Buffer P2 addition when using LyseBlue Reagent?
FAQ ID -861
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Will the FastLane Cell cDNA Kit work with suspension cells?
FAQ ID -801
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What publicly funded Genome Sequencing Projects has QIAGEN's Sequencing Service been involved in?
FAQ ID -815
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What is the typical yield of site-specific labeled protein generated using the EasyXpress Site-Specific Biotin Kit?
FAQ ID -873
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What programs does your Sequencing Service use to ensure accurate base calling, Sequence Quality, and Alignment?
FAQ ID -831
浏览
Where can I find a copy of the report file generated by running 'Maintenance Protocol I' on my BioRobot MDx?
FAQ ID -806
浏览
Which QIAGEN plasmid preparation kits will contain LyseBlue Reagent?
FAQ ID -865
浏览
Which fraction will contain soluble mitochondrial proteins using the Qproteome Cell Compartment Kit?
FAQ ID -839
浏览
Which fraction will contain endosomal, microsomal and lysosomal proteins using the Qproteome Cell Compartment Kit?
FAQ ID -808
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Do I need to use RNase inhibitors with the RNeasy Kits?
FAQ ID -813
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Where can I find QIAGEN products for a specific gene or gene product?
FAQ ID -803
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Do the Buffers CE1 - CE4 of the Qproteome Cell Compartment Kit contain DTT or any reducing agent?
FAQ ID -824
浏览
Can the FastLane Cell cDNA Kit also be used for qualitative end-point PCR?
FAQ ID -834
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Can the QuantiTect Primer Assays be used for detection of genomic DNA?
FAQ ID -811
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Can the FastLane Cell cDNA Kit be used with tissue?
FAQ ID -821
浏览
Do you have a protocol for the isolation of bacteriophage P1 derived constructs with any of your plasmid kits?
FAQ ID -883
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Do you have an in vitro translation kit allowing the incorporation of seleno-methionine for X-Ray crystallography based protein analysis?
FAQ ID -879
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895 - Do you have a protocol for the isolation of endotoxin-free plasmid DNA using the QIAGEN Plasmid Midi Kit?
浏览
Do you have a protocol for isolation of plasmid DNA using the Sigma Centrifuge 4?
-15
浏览
Do you have a protocol for the isolation of BAC DNA using the QIAGEN Plasmid Midi Kit?
FAQ ID -881
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Can I reuse the Ni-NTA Agarose and Ni-NTA Superflow resins?
FAQ ID -802
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Can Buffer R1 of the R.E.A.L. Prep 96 Plasmid Kit be replaced with Buffer P1?
FAQ ID -823
浏览
Are there specific recommendations for performing RT-PCR on RNA isolated from paraffin-embedded samples?
FAQ ID -828
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Can I stabilize adipose tissue using RNAprotect prior to RNA isolation?
FAQ ID -856
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Can I use the PhosphoProtein Purification Kit for the isolation of phosphorylated proteins from yeast?
FAQ ID -838
浏览
Can I use QIAGEN's pQE vectors as templates in the EasyXpress Biotin Labeling Kits?
FAQ ID -874
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Can I use LyseBlue with R.E.A.L. Prep 96, QIAwell Ultra, or QIAprep 96 Turbo Miniprep Kits for the BioRobot systems?
FAQ ID -863
浏览
Can I use my gene-specific primers with the FastLane Cell cDNA Kit and the QuantiTect Reverse Transcription Kit?
FAQ ID -812
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Have you tested the effect of inhibitors on PCR performance?
FAQ ID -818
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Do you sell the ATP-Dependent Exonuclease of the QIAGEN Large-Construct Kit separately?
FAQ ID -825
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How can I separate PCR fragments that are small and very close in size on an agarose gel?
FAQ ID -800
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Do you have information on in vivo RNA interference experiments?
FAQ ID -817
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I cannot see the LCD display fonts on the Sigma Centrifuge. How do I adjust the contrast?
FAQ ID -822
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I left Buffer P1 at room temperature after addition of RNase A, what shall I do?
FAQ ID -859
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How long does a typical in vitro translation reaction take with your large-scale EasyXpress protein synthesis kits?
FAQ ID -880
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How much protein can be produced with the EasyXpress Protein Synthesis Mega Kit?
FAQ ID -843
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Do you have a protocol for the isolation of very low-copy plasmids from Streptomyces spp.?
FAQ ID -893
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Do you have a protocol for the isolation of plasmid DNA from Citrobacter freundii?
FAQ ID -885
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Do you have a protocol for the isolation of plasmid DNA from Corynebacterium glutamicum?
FAQ ID -886
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Do you have a protocol for the isolation of plasmid DNA from Agrobacterium?
FAQ ID -898
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Do you have a protocol for the isolation of plasmid DNA from Borrelia spp.?
FAQ ID -884
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Do you have a protocol for the isolation of plasmid DNA from Bacillus subtilis?
FAQ ID -896
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Do you have a protocol for the isolation of plasmid DNA from yeast?
FAQ ID -853
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Do you have a protocol for the isolation of plasmid DNA from Lactobacillus spp.?
FAQ ID -887
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Do you have a protocol for the isolation of single-stranded DNA from M13 phage using QIAGEN Plasmid Kits?
FAQ ID -894
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Do you have a protocol for the isolation of plasmid DNA from Staphylococcus spp.?
FAQ ID -890
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Do you have a protocol for the isolation of plasmid DNA from Oligotropha carboxidovorans?
FAQ ID -888
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Do you have a protocol for the isolation of plasmid DNA from Proteus spp.?
FAQ ID -889
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What are the most commonly used protease inhibitors?
FAQ ID -53
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How should I quantify RNA isolated with RNeasy Kits?
FAQ ID -32
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Is plasmid DNA purified with QIAGEN Plasmid Purification Kits suitable for in vitro transcription?
FAQ ID -1
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How should I propagate pQE expression plasmids?
FAQ ID -58
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What are the compatibilities of different reagents with Ni-NTA matrices?
FAQ ID -49
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How is "Touchdown PCR" used to increase PCR specificity?
FAQ ID -75
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What should the starting template DNA quality and quantity be for PCR?
FAQ ID -74
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Why do I get smeared PCR products?
FAQ ID -87
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Why does my isolated RNA have a low OD 260/280 ratio?
FAQ ID -97
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When is chloramphenicol amplification of plasmids performed?
FAQ ID -3
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How can I remove imidazole from a protein sample?
FAQ ID -91
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How can I increase the amount of soluble recombinant protein in E. coli expression?
FAQ ID -64
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How can I avoid little or no RNA yields when using an RNeasy Kit?
FAQ ID -28
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What are the restriction sites of the pDrive Vector in the QIAGEN PCR Cloning Kit?
FAQ ID -160
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What are the molecular weights of proteins in the 6xHis Protein Ladder?
FAQ ID -169
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What are your recommendations for cotransfecting several plasmids?
FAQ ID -124
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What is the composition of Buffer EB?
FAQ ID -199
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Is mRNA isolation necessary for sensitive RT-PCR?
FAQ ID -111
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What are the recommended culture and buffer volumes for a very low-copy plasmid?
FAQ ID -168
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Should I use Ni-NTA Agarose in column or batch format for purification of 6xHis-tagged proteins?
FAQ ID -147
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What is the difference between disruption and homogenization in the RNeasy System?
FAQ ID -139
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Which Anti-His Antibody is the most sensitive for my protein of interest?
FAQ ID -172
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When should carrier be used with the QIAamp DNA Mini or the DNeasy Blood & Tissue Kit?
FAQ ID -100
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Why do I have to add beta-mercaptoethanol (beta-ME) to lysis Buffer RLT of the RNeasy Kits?
FAQ ID -101
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What is the recommended culture medium for the QIAprep System?
FAQ ID -154
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What is the RNase A concentration and composition of Buffer P1?
FAQ ID -198
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What is the genotype of the EZ Competent Cells?
FAQ ID -157
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What kind of PCR products can be cloned with the QIAGEN PCR Cloning Kit?
FAQ ID -165
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What is the principle behind Effectene Transfection Reagent?
FAQ ID -184
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What is the size of genomic DNA that is obtained with QIAGEN Genomic-tips?
FAQ ID -142
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How can I eliminate contaminating protein in my Ni-NTA 6xHis-tag protein purification?
FAQ ID -102
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How can I purify very small amounts of 6xHis-tagged protein using Ni-NTA technology?
FAQ ID -134
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How can I improve recoveries when using the QIAquick Kits?
FAQ ID -180
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How can I extract DNA from a polyacrylamide (PAGE) gel?
FAQ ID -120
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How can I improve ligation efficiency of DNA from a QIAEX II Gel Extraction Kit?
FAQ ID -141
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How can I isolate RNA from 1 gram of plant tissue?
FAQ ID -128
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How can I improve the performance of the HiSpeed QIAprecipitator module?
FAQ ID -144
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How can I improve transfection efficiency using Effectene Transfection Reagent?
FAQ ID -181
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What can I use to isolate RNA smaller than 200 nucleotides?
FAQ ID -115
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What epitopes do the Anti-His Conjugates and Anti-His Antibodies recognize?
FAQ ID -170
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What are the maximum culture volumes to use with the QIAGEN Plasmid Midi or Maxi Kit?
FAQ ID -167
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How should RNeasy Kits be stored and how long are they stable?
FAQ ID -103
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Is it possible to cleave the 6xHis-tag from an expressed protein?
FAQ ID -140
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How should I store plant material for DNA isolation using the DNeasy Plant Kit?
FAQ ID -114
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How should QIAGEN Plasmid Purification Kits be stored and for how long?
FAQ ID -192
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What are the features and benefits of the QIAexpress 6xHis Tag System?
FAQ ID -193
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What is the small band below my fragment of interest on an agarose gel after DNA cleanup using QIAquick?
FAQ ID -148
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Do you have transfection data for QIAGEN Transfection Reagents?
FAQ ID -158
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Do I need to use an RNase inhibitor in my RT reaction?
FAQ ID -119
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Do you have data showing effects of sample size on DNA yield and purity using the DNeasy 96 Blood & Tissue kit?
FAQ ID -129
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How can I avoid variable transfection efficiencies with SuperFect Reagent?
FAQ ID -179
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Does QIAGEN offer vectors for direct cloning of PCR products?
FAQ ID -146
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Can a QIAquick Gel Extraction Kit be used to obtain RNA from a formaldehyde gel?
FAQ ID -133
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Are QIAamp DNA isolation kits suitable for apoptosis studies?
FAQ ID -149
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Can DyeEx be used to clean up labeled DNA other than sequencing templates?
FAQ ID -137
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Are RNeasy spin columns sold separately?
FAQ ID -159
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Can I use QIAprep Miniprep kits for low-copy plasmids and cosmids?
FAQ ID -127
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Can I use the QIAquick PCR Purification Kit for restriction enzyme cleanup?
FAQ ID -130
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Can I use a QIAfilter Cartridge for purifying large plasmids?
FAQ ID -121
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Can I use Omniscript or Sensiscript RT's at a higher temperature?
FAQ ID -116
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How does imidazole affect my quantitation of protein?
FAQ ID -132
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How can I optimize the transfection of oligos and large plasmids using PolyFect Transfection Reagent?
FAQ ID -176
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How do you ensure that RNeasy buffers are RNase-free?
FAQ ID -113
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How can I purify DNA from soil, food and sewage samples for PCR?
FAQ ID -118
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How can I obtain DNA-free RNA using an RNeasy Midi or Maxi Kit?
FAQ ID -143
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How do I prepare an insert for pQE vectors?
FAQ ID -185
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How should I store Anti·His and Tag·100 Antibodies?
FAQ ID -194
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Do you have a protocol for purification of total DNA from insects?
FAQ ID -1254
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Is your Nuclease-Free Water fluorescence-free?
FAQ ID -1291
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Do you have a protocol for purification of total DNA from crude lysates?
FAQ ID -1255
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How should the GelPilot DNA Loading Dye be handled?
FAQ ID -1287
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What quality level does your Nuclease-Free Water have?
FAQ ID -1292
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What is the pH value of QIAGEN's Nuclease-Free Water?
FAQ ID -1290
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Does GelPilot DNA Loading Dye contain ethidium bromide?
FAQ ID -1286
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Are MaXtract tubes siliconized?
FAQ ID -1298
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Can phenol/chloroform be placed in the MaXtract tube for extraction at a later time?
FAQ ID -1299
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Should I input the dilution factor of my samples into the LiquiChip Analyser Software dilution factors of samples?
FAQ ID -1211
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What cell lines have been tested with the Qproteome Plasma Membrane Protein Kit?
FAQ ID -1205
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What are the new features of the EasyXpress Insect Kit II compared to the original Protein Synthesis Insect kit?
FAQ ID -1220
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What are your recommendations for PCR template preparation for use with the EasyXpress Insect Kit II?
FAQ ID -1221
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Is it possible to increase nucleic acid concentrations when following protocols on the BioRobot EZ1?
FAQ ID -1232
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Is a saliva collection device provided in the RNeasy Protect Saliva Mini Kit?
FAQ ID -1213
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How should I prepare buffy coat samples for use on the BioRobot EZ1?
FAQ ID -1249
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Is it possible to use QIAGEN's pQE-Trisystem Vector with the EasyXpress Insect Kit II?
FAQ ID -1225
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Is training of lab personnel included with the purchase of a BioRobot EZ1?
FAQ ID -1239
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Where can I find information about the worktable setup on the BioRobot EZ1?
FAQ ID -1243
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Is RNAprotect Cell Reagent provided in the RNeasy Protect Cell Kit available separately?
FAQ ID -1217
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What vector do you recommend as template for in-vitro protein expression with the EasyXpress Insect Kit II?
FAQ ID -1224
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What type of promoter should my template have for in-vitro protein expression with the EasyXpress Insect Kit II?
FAQ ID -1223
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What is the difference between various FlexiTube siRNAs listed for the same target gene, and which one should I choose?
FAQ ID -1206
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Will the Qproteome Plasma Membrane Protein Kit work with starting materials other than adherent mammalian cells?
FAQ ID -1202
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What disposables are required for nucleic acid isolations on the BioRobot EZ1?
FAQ ID -1246
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What is the protein yield when using the Qproteome Plasma Membrane Protein Kit?
FAQ ID -1201
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What is the maximum number of cells that can be used with the AllPrep RNA/Protein Kit ?
FAQ ID -1208
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Do the foils sealing the EZ1 Reagent Cartridges have to be manually removed prior to loading the robot?
FAQ ID -1240
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What is the smallest and the largest protein that you tested for expression with the EasyXpress Insect Kit II?
FAQ ID -1218
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What is the size of the BioRobot EZ1?
FAQ ID -1245
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Does anything have to be added to express post-translational modifications (PTMs) with the EasyXpress Insect Kit II?
FAQ ID -1226
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Do you have a protocol for isolating cytoplasmic RNA from animal cells using the RNeasy Mini Kit?
FAQ ID -1207
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Do you have a protocol for purification of miRNA from animal cells using the RNeasy Plus Mini Kit and RNeasy MinElute Cleanup Kit?
FAQ ID -1258
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Do you have a protocol for purification of cytoplasmic RNA from animal cells?
FAQ ID -1257
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Can cells stabilized with RNAprotect Cell Reagent be used for cell sorting by flow cytometry (FACS)?
FAQ ID -1216
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Can glycosylated proteins be expressed using the EasyXpress Insect Kit II?
FAQ ID -1227
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Can the Strep-Tactin Magnetic Beads of the Qproteome Plasma Membrane Protein Kit be reused?
FAQ ID -1204
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Can RNAprotect Saliva Reagent be purchased separately?
FAQ ID -1214
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Can you recommend a Reagent or Kit for stabilizing RNA in cultured cells?
FAQ ID -1215
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Can the RLT lysate of the BioSprint DNA Plant protocol be stored frozen?
FAQ ID -1248
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Can the Qproteome Plasma Membrane Protein Kit be used with tissue samples?
FAQ ID -1200
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How can we get our BioRobot EZ1 upgraded when new protocols are launched?
FAQ ID -1241
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How can I decontaminate the BioRobot EZ1?
FAQ ID -1231
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How much active protein can be expressed in-vitro using the EasyXpress Insect Kit II, and how much time does it take?
FAQ ID -1222
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How do you ensure that concentrated RNA is purified from saliva using the RNeasy Protect Saliva Mini Kit?
FAQ ID -1212
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Do you have a protocol for purification of total DNA from yeast?
FAQ ID -1253
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How many freeze-thaw cycles can the various reaction mixes in the EasyXpress Insect Kit II tolerate?
FAQ ID -1219
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Do you have a protocol for the isolation of DNA from buccal cells using the BioRobot EZ1?
FAQ ID -1260
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Do you have a protocol for transfection of suspension cell lines (Jurkat and K562) with siRNA?
FAQ ID -1250
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How are plasma membrane containing vesicles eluted off the ligand in the Qproteome Plasma Membrane Protein procedure?
FAQ ID -1203
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Do you have a protocol for transfection of differentiated macrophage cell lines (THP) with siRNA?
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Do you have a protocol for transfection of macrophage cell lines (J774.A1 and RAW 264.7) with siRNA?
FAQ ID -1251
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Can I use a centrifuge instead of vacuum when using the QIAquick 96 PCR Purification Kit?
FAQ ID -293
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If I want to use BioMag particles, do I have to buy a QIAGEN magnet too or can I use one that I already have?
FAQ ID -266
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Can the PAXgene Blood RNA Tubes undergo freeze/thaw cycles?
FAQ ID -2471
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Should I use Omniscript or Sensiscript for reverse transcription of low-copy mRNA?
FAQ ID -297
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I used to buy BioMag particles from a company by another name? Are your BioMag particles the same thing?
FAQ ID -263
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What are the main differences between other magnetic beads and the BioMag particles?
FAQ ID -272
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My BioMag is about to expire, is it still any good?
FAQ ID -268
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If I am using BioMag Streptavidin to capture biotinylated oligos, how can I then remove the oligos?
FAQ ID -279
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How magnetically responsive is BioMag?
FAQ ID -265
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What is the recipe for LB?
FAQ ID -212
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What is the maximum binding capacity of RNeasy spin columns?
FAQ ID -290
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What is the recipe for 2x YT?
FAQ ID -213
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Why does my DNA sample float out of the slot when loading it onto an agarose gel?
FAQ ID -205
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Why is an ice-incubation step included during reaction set-up when following the QuantiTect RT-PCR but not the QuantiTect PCR protocol.
FAQ ID -283
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What sequencing primers can I use with the pDrive cloning vector of the QIAGEN PCR Cloning Kit?
FAQ ID -292
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What is the composition of Buffer P2?
FAQ ID -203
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What is the difference between the QIAamp MinElute Virus Spin and the MinElute Virus Vacuum Kit?
FAQ ID -295
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What is the composition of the QIAGEN Multiplex PCR Buffer?
FAQ ID -289
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What is the difference between the Fc specific versions of your secondary antibody BioMag particles and the non-specific?
FAQ ID -275
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Can I use HEPES buffer instead of phosphate in my Ni-NTA column?
FAQ ID -291
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Can I use my own cycling conditions with the QIAGEN Multiplex PCR Kit?
FAQ ID -287
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Can I select FITC labeled cells with BioMag?
FAQ ID -278
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A white precipitate has formed in my 10x RT buffer. Is it still ok to use?
FAQ ID -216
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Can I use BioMag particles with any specific primary antibody?
FAQ ID -273
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Can I clean up my DNase treated RNA samples using RNeasy columns?
FAQ ID -286
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Are BioMag particles stable in other solvents?
FAQ ID -267
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Can BioMag be used for positive and negative selection?
FAQ ID -269
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How can I avoid poor immunolocalization morphology with Anti-His Antibodies?
FAQ ID -200
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For how long are BioMag Particles stable?
FAQ ID -262
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How can I prevent non-specific binding when using BioMag?
FAQ ID -277
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How do I prevent bubbles from forming in my Ni-NTA agarose column?
FAQ ID -285
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How can I purify mRNA from total RNA using an Oligotex Direct mRNA Kit?
FAQ ID -201
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How can one determine the optimal annealing temperature for a specific PCR assay?
FAQ ID -288
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Does QIAGEN sell Q-Solution separately?
FAQ ID -204
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What is the composition of Protein Precipitation Solution?
FAQ ID -2810
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What is the composition and concentration of Glycogen Solution in Gentra Puregene Kits?
FAQ ID -2812
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What is the composition of Buffer RDD?
FAQ ID -2800
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What is the composition of Cell Lysis Solution?
FAQ ID -2809
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What is the composition of DNA Hydration Solution in Gentra Puregene Kits?
FAQ ID -2813
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What is the composition of Cell Suspension Solution?
FAQ ID -2811
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Which heating block is recommended for the pyrosequencing annealing step?
FAQ ID -2838
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What is the recommended amplicon size for CpG assays?
FAQ ID -2825
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Which end of the PCR primer for pyrosequencing should be biotinylated?
FAQ ID -2839
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Which competent E. coli cells should I use to amplify the SureSilencing shRNA Plasmids?
FAQ ID -2890
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Which analyses can be performed with the PyroMark Q96 ID software version 1.0?
FAQ ID -2845
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Which kits can be used in combination with the PyroMark CpG Assays and PyroMark Custom Assays?
FAQ ID -2822
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What region of LINE gets targeted by the assay for PyroMark Q96 MD or PyroMark Q24?
-1
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What should be the single peak height for the PyroMark Control Oligo on the different PyroMark instruments?
FAQ ID -2852
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Does the same U1 promoter in the SureSilencing shRNA Plasmids express shRNA in human, mouse, and rat cell lines?
FAQ ID -2887
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What is the amplicon length of the PyroMark CpG LINE assay?
-1
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How are the PyroMark CpG Assays reconstituted?
FAQ ID -2817
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How can I use the SureSilencing shRNA Plasmids for stable transfection and knock down in a cell line that is already resistant to both neomycin and puromycin?
FAQ ID -2895
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Does the PyroMark LINE assay target all LINE sequences?
-1
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Can I use fluorescence microscopy to assess the transfection efficiency of the SureSilencing shRNA Plasmids with GFP in my model cell line of interest?
FAQ ID -2894
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Can I use my own siRNA design?
FAQ ID -2897
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