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Why do I see multiple high-intensity peaks in my qPCR dissociation curve at temperatures less than 70 ºC?
FAQ ID -2690
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Why do I sometimes get light blue colonies when using the QIAGEN PCR Cloning Kit?
FAQ ID -603
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Why do melting temperatures differ between PCR fragments amplified with QIAGEN's QuantiTect SYBR Green PCR Kits and Roche Kits?
FAQ ID -1084
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Why do my qPCR amplification curves or plots decrease in fluorescence intensity after the saturation phase?
FAQ ID -2689
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Why do pQE-T7 vectors of the QIAgenes E. coli system have an additional UAG amber stop codon?
FAQ ID -2043
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Why do qBiomarker Copy Number PCR Arrays have assays in quadruplicate?
FAQ ID — 3414
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Why do QuantiFast SYBR Green PCR Kits require such a high primer concentration?
FAQ ID -1443
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Why do replicates in real-time PCR have different plateau heights?
FAQ ID -539
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Why do the Internal Control templates for extraction (Internal Control DNA or RNA [High conc.]) have a 10x higher concentration than the IC templates provided with the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit?
FAQ ID -2603
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Why do you recommend using Triton X for the purification of 6xHis-tagged protein?
-100
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Why does AllPrep DNA/RNA/Protein Mini kit use buffer RLT, but Allprep DNA/RNA Mini and Allprep DNA/RNA Micro kits use buffer RLT Plus? Are these buffers interchangeable?
FAQ ID - 3391
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Why does an intensity calibration of the gel cartridge for the QIAxcel System and QIAxcel Advanced need to be performed?
FAQ ID -1824
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Why does my DNA sample float out of the slot when loading it onto an agarose gel?
FAQ ID -205
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Why does my isolated RNA have a low OD 260/280 ratio?
FAQ ID -97
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Why does my PCR product show up later when comparing the QuantiTect SYBR Green PCR Kits with Roche kits using the same annealing temperature?
FAQ ID -1083
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Why does my purified DNA/RNA sometimes have a 260/280 or 260/230 ratio of more than 2? Does that mean the purity is poor?
FAQ ID -3132
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Why does my realtime PCR assay quality decrease over time?
FAQ ID -589
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Why does TA/UA cloning work with your proofreading HotStar HiFidelity DNA Polymerase?
FAQ ID -1053
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Why does the Allprep DNA/RNA 96 Kit use Buffer RLT, whereas the AllPrep DNA/RNA Mini Kit uses Buffer RLT Plus?
FAQ ID -1999
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Why does the connection test fail?
FAQ ID - 141522
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Why does the copy function not apply relative to migration time and normalized area values after loading the DNA size marker table and adding the alignment marker peaks on the QIAxcel System?
FAQ ID -1832
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Why does the DNeasy mericon Food extraction kit use a QIAquick column and not DNeasy column?
FAQ ID -3148
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Why does the DNeasy mericon Food Kit use a QIAquick column and Buffer PB rather than a DNeasy column and Buffer AL, which might be expected since the kit isolates genomic DNA?
FAQ ID - 3347
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Why does the miScript Target Protector not induce the degradation process?
FAQ ID -2263
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Why does the Power LED not function with the QIAxcel or QIAxcel Advanced instrument plugged in and the main power switch turned on?
FAQ ID -1851
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Why does the QIAamp DNA Mini Tissue Protocol require both ATL and AL buffer, while the Blood Protocol only uses AL?
FAQ ID -633
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Why does the QIAcube sometimes fail to pick up the tips or pick up wrong tips?
FAQ ID -3131
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Why does the QuantiTect Primer Assay for my gene of interest have only one version number?
FAQ ID -1134
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Why does the SeqTarget Prep Protocol require a 2-step procedure?
FAQ ID -2238
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Why does the template for EasyXpress Disulfide Insect Kits need to have a signal sequence (such as the mellitin signal sequence as provided by the EasyXpress Linear Template Fab Kit primers)?
FAQ ID -2967
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Why does the upper aqueous phase look pinkish when purifying RNA from fatty tissue?
FAQ ID -3118
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Why had my RT² SYBR Green Mastermix been working well in the past, but now does not seem to be?
FAQ ID -2717
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Why have the hazard symbols accompanying products changed?
FAQ ID - 3430
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Why is 'NO COM' displayed on the QIAxcel Instrument Panel although the power is on and all connections are made to the QIAxcel instrument and computer?
FAQ ID -1848
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Why is 18S ribosomal RNA (rRNA) used as a housekeeping gene to normalize sample-to-sample, systematic variation in qPCR assays?
FAQ ID -2675
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Why is a 2-step (and not a 3-step) cycling protocol recommended for QuantiFast SYBR Green PCR Kits?
FAQ ID -1450
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Why is a 2-step (and not a 3-step) cycling protocol recommended for Rotor-Gene SYBR Green Kits?
FAQ ID -2122
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Why is a blood collection set required to be used with the PAXgene Blood RNA tube?
FAQ ID - 3459
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Why is a blood collection set required to collect blood into the PAXgene Blood ccfDNA Tube?
FAQ ID - 3628
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Why is an ice-incubation step included during reaction set-up when following the QuantiTect RT-PCR but not the QuantiTect PCR protocol.
FAQ ID -283
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Why is carrier RNA used during the isolation of gDNA from microdissected samples with the QIAamp DNA Micro Kit?
FAQ ID -473
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Why is CoralLoad included in the Type-it Mutation Detect, but not in the Type-it Microsatellite PCR Kit?
FAQ ID -2068
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Why is DTT used only for semen samples?
FAQ ID -
143761
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Why is EvaGreen instead of SYBR Green used as fluorescent dye in the Type-it HRM PCR Kit?
FAQ ID -2196
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Why is it not recommended to stabilize cells with RNAprotect?
FAQ ID -941
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Why is it recommended to add 1 mM IPTG for optimal protein yields using the EasyXpress Protein Synthesis Kits?
FAQ ID -860
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Why is maximum amplicon size for the Type-it Microsatellite PCR Kit limited to 500 bp?
FAQ ID -2060
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Why is my 260/280 ratio low after using the DNeasy mericon Food Kit?
FAQ ID - 3349
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Why is my EpiTect Methyl qPCR Assay "failed" as indicated in the QC page of the data analysis Excel file?
FAQ ID -2734
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Why is my no template control (NTC) real-time Ct value < 35 cycles in my qPCR Assay?
FAQ ID -2686
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Why is my plasmid DNA yield low?
FAQ ID -768
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Why is special Type-it Fast SNP Probe PCR chemistry required for TaqMan® SNP Genotyping?
FAQ ID -2057
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Why is the activation time for HotStarTaq Plus Polymerase in the QuantiFast SYBR Green Kits different from that for QuantiFast Probe Kits?
FAQ ID -1449
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Why is the alignment marker for the 1.8 kb fragment not visible when using the 25 bp/1.8 kb DNA Size Marker together with the 15 bp/400 bp marker on the QIAxcel System and QIAxcel Advanced?
FAQ ID -1835
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Why is the copy number call lower than expected?
FAQ ID — 3413
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Why is the final heat denaturation step so important with the QIAsymphony PAXgene Blood RNA protocol?
FAQ ID -2987
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Why is the fluorescence signal of the Internal Control on my Rotor-Gene Q very low?
FAQ ID -2574
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Why is the initial activation step different for Rotor-Gene Probe, SYBR Green and Multiplex Kits?
FAQ ID -2118
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Why is the pQE DNA provided in QIAexpress Kits blue in color?
FAQ ID -487
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Why is the QIAamp Viral RNA Mini Kit (50) currently unavailable?
FAQ ID -147395
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Why is the QIAcube Connect App reacting so slowly?
FAQ ID - 141519
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Why is the qRT-PCR reproducibility so critical when detecting gene expression knock down in an RNAi experiment?
FAQ ID -2774
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Why is the QuantiFast denaturation step different for PCR and RT-PCR runs in the two-step protocol for the ABI 7500 and other cyclers?
FAQ ID -1442
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Why is the reaction volume for QuantiFast PCR Kits lower than that for QuantiTect PCR Kits?
FAQ ID -1447
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Why is the RT step with the QuantiFast RT Kits much shorter compared to QuantiTect RT Kits?
FAQ ID -1451
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Why is the storage time for QuantiFast PCR Kits shorter than that for QuantiTect PCR Kits?
FAQ ID -1446
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Why is the Type-it Mutation Detect PCR Kit recommended for preamplification of SNPs?
FAQ ID -2067
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Why is there an asterisk for my miScript Primer Assays?
FAQ ID -2176
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Why is there DNA in the no-template (negative) control when using the QuantiTect Whole Transcriptome Kit?
FAQ ID -1620
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Why is there DNA in the no-template control reaction when using the standard REPLI-g procedure, but not when using the UltraFast procedure?
FAQ ID -1327
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Why is there more than one QIAgene for my gene of interest?
FAQ ID -2044
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Why is there no detection of free reporter fluorescence in the LiquiChip System?
FAQ ID -732
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Why is there no male DNA obtained from sexual assault samples?
FAQ ID -
143767
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Why is there no QuantiTect Primer Assay for my gene of interest?
FAQ ID -1140
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Why is there no vacuum-only protocol for the RNeasy Plus 96 Kit?
FAQ ID -1993
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Why is there RNA in the DNA eluate when using the AllPrep DNA Mini Spin Column?
FAQ ID -1044
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Why might Affymetrix GeneChip Mapping assays interfere with the REPLI-g FFPE kit?
FAQ ID -1755
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Why might my gene of interest drop out during WGA if it is not near a telomere or centromere?
FAQ ID -703
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Why should DNA or cDNA targets be less than 250 bp long for real-time PCR?
FAQ ID-751
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Why should I use RT² SYBR Green Mastermix with RT² qPCR Primer Assays?
FAQ ID -2706
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Why should I use the EpiTect Plus FFPE Bisulfite Kit instead of isolating FFPE DNA first and then doing the bisulfite conversion?
FAQ ID -2412
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Why should I use the RNeasy Microarray Tissue Mini Kit when purifying RNA for microarray analysis?
FAQ ID -2188
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Why should I work with diluted or undiluted screens?
FAQ ID -2419
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Why would clumps occur following the addition of Buffer P2 when using LyseBlue Reagent in a plasmid preparation?
FAQ ID -862
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Will acetone precipitation recommended in the Qproteome Protocols denature Protein?
FAQ ID -807
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Will biotin detection assays be efficient when doing in vitro translation from E. coli lysates with the EasyXpress Site-Specific Biotin Kit?
FAQ ID -878
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Will DNA from chromosomes collected in TE with a drop of mineral oil work with REPLI-g amplification?
FAQ ID -664
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Will dUTP in a PCR reaction affect pyrosequencing?
FAQ ID -2843
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Will integral, peripheral as well as lipid-anchored plasma membrane proteins be isolated with the Qproteome Plasma Membrane Protein Kit?
FAQ ID -1199
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Will mitochondrial DNA also be isolated using the DNA tissue protocols and QIAsymphony DSP DNA Mini Kit on the QIAsymphony SP?
FAQ ID -2991
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Will mitochondrial DNA also be isolated using the DNA Tissue protocols on the QIAsymphony SP?
FAQ ID -1921
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Will pancreas tissue swim on top of RNAprotect?
FAQ ID -536
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Will pipetting error affect the EpiTect Methyl qPCR Array results?
FAQ ID -2741
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Will pipetting error affect the miRNA qPCR Assay results?
FAQ ID -2728
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Will proteins eluted in the Qproteome Plasma Membrane Protein protocol be modified, e.g. with the ligand?
FAQ ID -1195
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Will qBiomarker Copy Number Assays work with heterogeneous samples like mixtures of tumor and normal tissue?
FAQ ID — 3419
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Will QIAseq FastSelect work in other species?
FAQ ID -147911
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Will QuantiTect Primer Assays work at an annealing temperature of 60ºC with QuantiFast SYBR Green PCR and RT-PCR Kits?
FAQ ID -1553
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Will QuantiTect Primer Assays work with Rotor-Gene SYBR Green Kits using an annealing step at 60ºC?
FAQ ID -2124
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Will REPLI-g work at high temperatures?
FAQ ID -656
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Will Rotor-Gene Kits also work on the Rotor-Gene 6000 and 3000 cyclers?
FAQ ID -2121
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Will site-specific biotin labeling with the EasyXpress Site-Specific Biotin kit alter the structure and function of my protein?
FAQ ID -877
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Will TA cloning work with REPLI-g WGA product?
FAQ ID -672
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Will the "classic" RNeasy Mini Kit be discontinued after the launch of the Allprep DNA/RNA Mini Kit?
FAQ ID -1054
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Will the 2x QIAGEN Multiplex PCR Master Mix freeze at -20°C?
FAQ ID - 525
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Will the FastLane Cell cDNA Kit work with suspension cells?
FAQ ID -801
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Will the ligand in the Qproteome Plasma Membrane Protein protocol be specific for plasma membrane exclusively?
FAQ ID -1198
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Will the optimized DNA sequence for QIAgenes be provided?
FAQ ID -2045
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Will the PhosphoSerine antibody recognize Serine residues if the neighboring residues are Leucine or Isoleucine?
FAQ ID -644
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Will the primer sequence for the PyroMark CpG Assay be provided?
FAQ ID -2823
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Will the QIAquick PCR Purification Kit remove sufficient SYBR Green from real-time PCR reactions to allow sequencing?
FAQ ID -637
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Will the QIAxcel System run using a green Data Review Software Key?
FAQ ID -1853
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Will the Qproteome Plasma Membrane Protein Kit work with starting materials other than adherent mammalian cells?
FAQ ID -1202
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Will the random hexamers in the REPLI-g reaction interfere with downstream analysis?
FAQ ID -713
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Will the REPLI-g UltraFast Mini Kit reaction always yield 10 µg DNA?
FAQ ID -1328
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Will the Reverse transcription control on the RT2 profiler PCR array work on any cDNA library?
FAQ ID - 3534
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Will the sequence of the QuantiFast Probe Assays be provided?
FAQ ID -2369
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Will the sequences of the QuantiTect Primer Assays be provided?
FAQ ID -804
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Will Uracil-N-Glycosylase (UNG) completely remove contaminating amplicons when using QuantiTect +UNG Kits?
FAQ ID -2139
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With LyseBlue reagent for lysis control, can I now process more bacterial culture and overload the columns?
FAQ ID -864
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With which platform can I perform my sequencing?
FAQ ID -
143072
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With which read length should the EpiTect Hi-C NGS libraries be sequenced
FAQ ID -
143073
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Would co-transfection with the plasmid be expected to increase/decrease the transfection efficiency significantly?
FAQ ID -3136
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