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How does the QuantiFast Probe RT-PCR Plus Kit eliminate genomic DNA contamination?
FAQ ID -2354
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How do I analyze the data for the particular iPSC qBiomarker Arrays I have ordered?
FAQ ID -2440
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How are the genes in each iPSC qBiomarker PCR Array selected?
FAQ ID -2439
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How can I adjust the ROX concentration in the QuantiFast Probe RT-PCR Plus master mix?
FAQ ID -2356
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How long can I store my purified DNA/RNA isolated with the AllPrep DNA/RNA FFPE kit?
FAQ ID -2350
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How is the RNeasy Plus Universal Kit different from the RNeasy Plus Kit?
FAQ ID -2340
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How do I harvest the crystals from meso phase? Are special tools needed with the NexTal Cubic Phase products?
FAQ ID -2330
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Is a special processing protocol needed?
FAQ ID -2523
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What is the detection limit for the Investigator Quantiplex Kit?
FAQ ID -2572
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Is it possible to archive PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue blocks?
FAQ ID -2524
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The disruption of my sample worked well with the TissueLyser LT, but I am not satisfied with the RNA yield. What do you suggest?
FAQ ID -2555
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What are the dimensions of the TissueLyser LT?
FAQ ID -2545
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On which cyclers can the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit be used?
FAQ ID -2596
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What is the average RNA yield from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -2534
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What do I have to consider if I am using Applied Biosystems SDS software version 1.2.3 to run an Investigator Quantiplex reaction?
FAQ ID -2570
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What is in the elution buffer in the PAXgene Bone Marrow RNA kit?
FAQ ID -2511
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What are the storage conditions for the mericon Assay kits?
FAQ ID -2540
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Is the performance of the HiPerFect HTS Reagent affected if it has accidentally been stored at°C?
-20
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Is it possible to embed PAXgene Tissue fixed and stabilized samples in Optimal Cutting Temperature (OCT) medium for freezing?
FAQ ID -2525
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Is it possible to microdissect PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissues?
FAQ ID -2531
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Is the morphology after H&E staining comparable to formalin-fixed samples?
FAQ ID -2526
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Is RNA from red blood cells also isolated when RNA is extracted from a PAXgene Bone Marrow RNA Tube?
FAQ ID -2509
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Is the 260/230 nm absorbance ratio specified for RNA derived from the PAXgene Bone Marrow RNA System?
FAQ ID -2514
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Which version of the Rotor-Gene Q software can be used to run the Investigator Quantiplex?
FAQ ID -2568
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What is the maximum tissue size that can be fixed in a PAXgene Tissue Container?
FAQ ID -2518
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Why do I observe a CT shift for the Internal Control in some samples in comparison to the Ct value for the Internal Control of the dilution series of the Control DNA Z1?
FAQ ID -2575
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What RNA yields are expected with the PAXgene Bone Marrow RNA System?
FAQ ID -2506
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What is the RT-PCR performance of RNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissue compared to RNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?
FAQ ID -2538
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What is the nature of the heterologous Internal Amplification Control and Positive Control in the mericon Assay kits?
FAQ ID -2543
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What is the expected amplicon size when using the mericon Assay kits?
FAQ ID -2544
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What is the longest period you recommend to leave out the dilute HiPerFect HTS at RT before use?
FAQ ID -2560
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What is the typical amount of tissue used for disruption with the TissueLyser LT?
FAQ ID -2552
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What kind of sample tubes are recommended to use with the TissueLyser LT?
FAQ ID -2553
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What is the purity of nucleic acids extracted with the PAXgene Tissue kits?
FAQ ID -2533
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Can the PAXgene Bone Marrow RNA System be used to isolate genomic DNA or viral RNA and DNA?
FAQ ID -2507
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Can the PAXgene Bone Marrow RNA System be used with animal bone marrow or with blood samples?
FAQ ID -2501
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Can the PAXgene RNA stabilization solution/bone marrow mixture from the PAXgene Bone Marrow RNA Tube be disposed of by adding bleach?
FAQ ID -2515
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Can sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for other histochemical staining techniques, such as PAS?
FAQ ID -2530
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Can the PAXgene Bone Marrow RNA System be used for other human body fluids than bone marrow samples or cultured cells of human origin?
FAQ ID -2502
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Can the sample tubes and the TissueLyser LT Adapter be cooled with liquid nitrogen?
FAQ ID -2549
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Can the TissueLyser LT Adapter be removed for cleaning?
FAQ ID -2546
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Can the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit be used with hybridization probes?
FAQ ID -2595
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Can the samples on the TissueLyser LT be protected from degradation of nucleic acids or proteins?
FAQ ID -2548
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When using HiPerFect HTS Reagent is it necessary to optimize amount of siRNA and transfection reagent?
FAQ ID -2556
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When using the QuantiFast Pathogen PCR +IC Kit or the QuantiFast Pathogen RT-PCR +IC Kit, what should the fluorescent label of the probe be for the customer-defined assay to detect the pathogen?
FAQ ID -2594
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Which human target is used for the quantification in the Investigator Quantiplex Kit?
FAQ ID -2577
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What sample numbers can be processed on the TissueLyser LT?
FAQ ID -2547
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What size bead should I use for tougher samples with the TissueLyser LT?
FAQ ID -2554
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When performing the Internal Control assay for the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit, what channel or filter can be used to detect MAX?
FAQ ID -2597
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Why is the fluorescence signal of the Internal Control on my Rotor-Gene Q very low?
FAQ ID -2574
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Which version of the Applied Biosystems 7500 Real-Time PCR System software can be used to run the Investigator Quantiplex?
FAQ ID -2569
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Can specific types of white blood cells be enriched from a PAXgene Bone Marrow RNA Tube prior to the RNA isolation?
FAQ ID -2508
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Are there any accessories required for sample disruption with the TissueLyser LT?
FAQ ID -2551
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At what temperature should I store the Investigator Quantiplex Kit?
FAQ ID -2578
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Can bone marrow aliquots other than 2 mL be pipetted into the PAXgene Bone Marrow RNA Tube?
FAQ ID -2503
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Are the Internal Controls used in the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit homologous to a known target?
FAQ ID -2598
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Can I use water to elute the RNA in the PAXgene Bone Marrow RNA Kit?
FAQ ID -2512
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Can bone marrow from leukemia patients with elevated numbers of white blood cells be processed with the PAXgene Bone Marrow RNA System?
FAQ ID -2510
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Can PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for in situ hybridization?
FAQ ID -2529
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How long is the fixation time?
FAQ ID -2519
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Can HiPerFect HTS be used for miRNA?
FAQ ID -2561
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Can HiPerFect HTS Reagent be used for transfecting shRNAs?
FAQ ID -2559
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How long does the sample disruption with the TissueLyser LT take?
FAQ ID -2550
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How many times can the Internal Controls used in the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit be freeze-thawed?
FAQ ID -2599
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How long can I store the dilutions of the Control DNA Z1 standards at 2°C?
-8
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How long can primer and probes from the mericon Assay kits be kept at°C once reconstituted?
-20
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How should the PAXgene Bone Marrow RNA Tubes be frozen after collection and for how long?
FAQ ID -2505
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How long does an Investigator Quantiplex run take?
FAQ ID -2566
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How much time may the aspirated bone marrow sample be allowed to incubate before pipetting it into the PAXgene Bone Marrow RNA Tube?
FAQ ID -2504
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I routinely heat an aliquot of the RNA eluate prior to my RT reaction. Do I still need to heat the eluate after the PAXgene Bone Marrow RNA preparation?
FAQ ID -2513
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Do I have to calibrate new dyes on my Applied Biosystems 7500 or 7500 Fast Real-Time PCR System?
FAQ ID -2571
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Do you have FACS or Fluoro-Microscopy data using HiPerFect HTS Reagent for delivery of siRNA?
FAQ ID -2557
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How can I streamline the quantification workflow for the Investigator QuantiPlex Kit?
FAQ ID -2579
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How is stability and performance of these mericon Assay kits guaranteed?
FAQ ID -2542
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How long are the target sequences of Investigator Quantiplex?
FAQ ID -2576
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For which real-time cyclers has the Investigator Quantiplex Kit been validated?
FAQ ID -2567
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How are the supernatants resulting from the first two spin steps of the PAXgene Bone Marrow RNA kit purification procedure disposed?
FAQ ID -2516
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What is the part number for O-rings for QIAsymphony SP and AS instruments? How often should the O-rings be replaced?
FAQ ID -3055
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Where can I find the ordering information for QIAcube accessories such as Shaker Rack plugs, Rack labeling strips, Reagent bottle rack, 30mL Reagent bottles, Rotor Adapter holder, Rotor adapters, Sample tubes CB, Sample tubes RB and Spin column adapter rings?
FAQ ID -3054
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Why are two reagents used in the PAXgene Tissue System?
FAQ ID -3022
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What is the maximum size of tissue that can be fixed in a PAXgene Tissue FIX Container (50 ml)?
FAQ ID -3024
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5. What is the maximum size of the tissue sample that can be fixed in a PAXgene Tissue Container?
FAQ ID -3023
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What happens if a tissue sample larger than 20 x 20 x 20 mm is used for fixation with the PAXgene Tissue FIX Container (50 ml)?
FAQ ID -3025
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Is it possible to fix over a weekend?
FAQ ID -3027
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How long is the fixation time when using PAXgene Tissue FIX Container?
FAQ ID -3026
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How long can a tissue specimen stay in the PAXgene Tissue STABILIZER?
FAQ ID -3028
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What is the minimum incubation time required when using the PAXgene Tissue STABILIZER?
FAQ ID -3029
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Is it possible to integrate the PAXgene Tissue STABILIZER into the processing?
FAQ ID -3037
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Is it possible to archive PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3038
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Is the morphology after H&E staining comparable to formalin-fixed samples?
FAQ ID -3039
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Are immunohistochemistry (IHC) assays developed for formalin-fixed, paraffin-embedded (FFPE) compatible with PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3040
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Is it possible to use formalin-fixed, paraffin-embedded (FFPE) kits and protocols for extraction of biomolecules from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3043
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is the purity of nucleic acids isolated with the PAXgene Tissue Kits?
FAQ ID -3044
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What is the average RNA yield from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3045
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What is the integrity of RNA from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3046
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What is the RT-PCR performance of RNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue compared to RNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?
FAQ ID -3050
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How can proteins purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be analyzed?
FAQ ID -3052
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What is the PCR performance of DNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue compared to DNA from snap frozen or formalin-fixed, paraffin-embedded (FFPE) tissue?
FAQ ID -3051
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What is the DNA integrity from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3047
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Does the PAXgene Tissue RNA Kit co-purify small RNAs?
FAQ ID -3048
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How can I extract proteins from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
FAQ ID -3049
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Can PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for in situ hybridization?
FAQ ID -3042
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What tubes can be used with TissueLyser Adapter Set 2 x 96 (cat. no. 69984)?
FAQ ID -3086
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What is the concentration of the Gentra RNase A that is used with the Autopure and Puregene protocols?
FAQ ID -3083
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What are the typical yields obtained from Buccal swabs on BioSprint 96?
FAQ ID -3071
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Where can I find a copy of the .kpf file for a BioSprint protocol?
FAQ ID -3073
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Can the DyeEx 96 kit be used to clean sequencing reactions to be run on ABI 3730?
FAQ ID -3075
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Can the FastLane RT reaction be incubated longer than 30 min at 42 deg C?
FAQ ID -3079
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Does carrier RNA interfere with the QuantiTect Whole Transcriptome amplification?
FAQ ID -3080
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What are the advantages and disadvantages of turning on the tip “re-use” feature on the QIAgility?
FAQ ID -3064
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How precise is the QIAgility?
FAQ ID -3063
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How long does it take to set up a 96-well plate PCR reaction on the QIAgility?
FAQ ID -3066
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Can I use a bar code scanner or reader on the QIAgility?
FAQ ID -3067
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Can I use a USB drive to transfer protocols to BioSprint?
FAQ ID -3072
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Can BioSprint 96 be connected to LIMS?
FAQ ID -3070
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How can I find the run parameters for a BioSprint protocol that I am running?
FAQ ID -3069
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Can the FastLane Cell lysates (containing stabilized RNA, step 8. in Fastlane protocol) be stored?
FAQ ID -3077
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Can I use specific primers with the FastLane Kits?
FAQ ID -3078
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What is the expected level of endotoxins in plasmid DNA purified with QIAGEN Plasmid kits, QIAfilter Plasmid kits and HiSpeed Plasmid purification kits?
FAQ ID -3082
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What is the expected level of endotoxins in plasmid DNA purified with QIAprep spin miniprep kit?
FAQ ID -3081
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What is the concentration of the different Proteinase K solutions sold by QIAGEN?
FAQ ID -3084
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Where can I access Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit?
FAQ ID -3085
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Is it possible to use a standard processor — the kind used routinely for formalin-fixed samples — for dehydration and paraffin infiltration of PAXgene Tissue treated samples?
FAQ ID -3032
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Are PAXgene Tissue Containers suitable for long term-storage at freezing temperatures?
FAQ ID -3030
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Is it possible to manually process PAXgene Tissue treated samples without using an automated processor?
FAQ ID -3036
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Is a special processing protocol needed?
FAQ ID -3035
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Is it possible to process formalin-fixed and PAXgene Tissue fixed samples together in one run?
FAQ ID -3033
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Is it necessary to clean a processor normally used for formalin-fixed tissue before using it with PAXgene Tissue fixed tissue?
FAQ ID -3034
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Can sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for other histochemical staining techniques, such as PAS?
FAQ ID -3041
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When should the N2 bottle used with the QIAxcel Advanced System be changed?
FAQ ID -3001
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Are the multicopy reference assays for Type-it CNV PCR +qC Kits and the qBiomarker assays the same?
FAQ ID -3000
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How should I reconstitute the lyophilized RNase-Free DNase Set with the RNeasy 96 kit?
FAQ ID -3003
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How do I clean up RNA preparations containing miRNA?
FAQ ID -3002
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What is the thermal output for a BioRobot MDx and BioRobot Universal?
FAQ ID -3056
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What is the thermal output for a QIAsymphony SP and AS instrument?
FAQ ID -3057
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Can I use Tungsten Carbide beads for RNA extraction protocols using QIAGEN Kits?
FAQ ID -3053
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What is the minimum number of samples that can be extracted on a BioSprint 96?
FAQ ID -3076
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What is the fixation method used in the PAXgene Tissue System?
FAQ ID -3019
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What does PAXgene Tissue FIX contain?
FAQ ID -3020
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Can I perform the IDH 1/2 RGQ PCR test with slides containing less than 40% tumor cells?
FAQ ID -3098
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What volume of bacterial culture can be processed using the QuickLyse Miniprep Kit?
FAQ ID -1357
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Can the MaXtract High Density Tube be used with QIAzol to isolate RNA?
FAQ ID -1304
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I have a Human Druggable Genome siRNA Set V2.0. Can I reorder the siRNAs from this set?
FAQ ID -1366
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What factors determine if denatured proteins accumulate underneath, or inside the gel of the MaXtract Low and High Density Tubes?
FAQ ID -1312
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If both MaXtract Low Density and High Density is suitable for an application, which type is preferable to use?
FAQ ID -1310
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How will service work carried out on my QIAGEN BioRobot be documented?
FAQ ID -1331
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If I cannot order FlexiPlate siRNA online, how do I order?
FAQ ID -1371
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What information about my gene or siRNA do I need to order FlexiPlate siRNA?
FAQ ID -1365
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Is it possible to use MaXtract for an organic extraction with a mixture of phenol and BCP (1 bromine chlorpropane)?
-3
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What information is provided with FlexiPlate siRNAs?
FAQ ID -1363
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Is it possible to elute plasmid DNA obtained with the QuickLyse Miniprep Kit in nuclease-free water?
FAQ ID -1355
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Which formats are available for FlexiPlate siRNA?
FAQ ID -1358
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Which parts are exchanged during a Preventive Maintenance visit for my QIAGEN BioRobot?
FAQ ID -1334
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When isolating DNA from plant cells using CTAB, should MaXtract Low or High Density be used?
FAQ ID -1308
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Which controls can I choose for FlexiPlate siRNA?
FAQ ID -1368
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Will the REPLI-g UltraFast Mini Kit reaction always yield 10 µg DNA?
FAQ ID -1328
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Why is there DNA in the no-template control reaction when using the standard REPLI-g procedure, but not when using the UltraFast procedure?
FAQ ID -1327
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Which density gel type is contained in the yellow or green MaXtract Tubes?
FAQ ID -1315
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What is the composition of FlexiPlate siRNA buffer?
FAQ ID -1361
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Which software updates are included in a Premium Cover Agreement for my QIAGEN Instrument?
FAQ ID -1336
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What is the composition of elution buffer QLE in the QuickLyse Miniprep Kit?
FAQ ID -1356
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Can I reduce the amount of starting material below 10 ng when using the REPLI-g UltraFast Mini Kit?
FAQ ID -1325
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Can MaXtract tubes be autoclaved prior to use?
FAQ ID -1305
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Can I reduce the reaction time to e.g., 30 minutes, when using the REPLI-g UltraFast Mini Kit?
FAQ ID -1326
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Can I use any type of DNA extraction method to purify template DNA for amplification using the REPLI-g Mitochondrial DNA Kit?
FAQ ID -1321
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Can the bacterial pellet in the QuickLyse Miniprep procedure be resuspended by pipetting up and down or shortening the vortexing time?
FAQ ID -1352
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Can several extraction steps be performed in the same MaXtract tube?
FAQ ID -1302
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Can a phenol-chloroform ratio different from 1:1 also be used with MaXtract Low and High Density?
FAQ ID -1309
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Can DNA isolated with MaXtract tubes be used for downstream applications such as restriction digestion, labeling, blotting, PCR, and automated sequencing?
FAQ ID -1303
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Can I amplify mitochondrial DNA directly from whole blood or cells using the REPLI-g Mitochondrial DNA Kit?
FAQ ID -1319
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Can I order Alexa Fluor labeled controls with FlexiPlate siRNA?
FAQ ID -1369
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Can frozen sample material pulverized in liquid nitrogen be directly added to MaXtract tubes?
FAQ ID -1301
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Can HP Validated siRNAs be ordered on the FlexiPlate?
FAQ ID -1367
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Can I also screen large constructs like BACs, fosmids, and cosmids using the QIAGEN PlasmidAmp Kit?
FAQ ID -1337
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How do I resuspend my FlexiPlate siRNAs?
FAQ ID -1360
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How much REPLI-g amplified mitochondrial DNA should be used in a subsequent PCR reaction?
FAQ ID -1320
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Do mutations in the target mitochondrial DNA prevent primer binding, resulting in no DNA amplification with the REPLI-g Mitochondrial DNA Kit?
FAQ ID -1323
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Do you have stability data for genomic DNA isolated with the BioSprint Kits?
FAQ ID -1318
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Does the addition of sample and organic solution to the MaXtract Tubes require a specific pipetting sequence?
FAQ ID -1307
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Do you have stability data for genomic DNA isolated with the Flexigene DNA Kit?
FAQ ID -1317
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How does a Service Support Agreement provide defined budgeting for maintaining my BioRobot?
FAQ ID -1330
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How can I upload my gene list for FlexiPlate siRNA to GeneGlobe?
FAQ ID -1364
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How can I increase the DNA yield from low-copy plasmids using the QuickLyse Miniprep Kit?
FAQ ID -1354
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How can I ensure optimal performance of my QIAGEN instrument?
FAQ ID -1329
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What is the "seed sequence" of a miRNA?
FAQ ID -2262
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What is the turn around time for the ordering of miScript Target Protector?
FAQ ID -2254
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What is the sample throughput of Pyrosequencing systems?
FAQ ID -2215
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What is the processing time for 96 normalizations with the SeqTarget System?
FAQ ID -2237
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What is the optimal range of PCR fragment sizes for normalization with the SeqTarget System?
FAQ ID -2247
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What method do you recommend to quantify DNA before running the KRAS and EGFR PCR kit?
FAQ ID -2292
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What technologies are used by the KRAS and EGFR PCR kits?
FAQ ID -2286
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What kind of automation can be done with the EasyXtal 15-well Tool?
FAQ ID -2220
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What kind of reading length can I expect when using Pyrosequencing technology for sequence analysis?
FAQ ID -2216
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What dyes are used in the KRAS and EGFR PCR kits?
FAQ ID -2288
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What do I do if I get amplification in my no template control (NTC) with the KRAS and EGFR PCR kits?
FAQ ID -2298
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What format are the EasyXtal 15-well tools?
FAQ ID -2219
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What do I need to provide when ordering a miScript Target Protector?
FAQ ID -2251
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What is the difference of the sequence of a miScript miRNA Inhibitor and miScript Target Protector?
FAQ ID -2253
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What is a miScript Precursor Assay?
FAQ ID -2214
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What is the difference between probability and typicality in the Rotor-Gene ScreenClust HRM Software?
FAQ ID -2203
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What is a miScript Target Protector?
FAQ ID -2249
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Which transfection reagent is recommended for transfection of the miScript Target Protectors?
FAQ ID -2252
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Which variants of the EasyXtal 15-well tool are available?
FAQ ID -2221
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Why are most of my samples outside of the cluster in supervised mode using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2205
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Why are some of my samples outside of the cluster using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2204
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Why does the SeqTarget Prep Protocol require a 2-step procedure?
FAQ ID -2238
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Why does the miScript Target Protector not induce the degradation process?
FAQ ID -2263
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Why do I have wavy DNA bands on my agarose gel?
FAQ ID -2260
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What will happen, if the normalized DNA for the SeqTarget System is eluted with Buffer EB instead of EB2?
FAQ ID -2242
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When negative results are generated using the KRAS and EGFR PCR kits, do I rerun them?
FAQ ID -2294
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What type of samples can I use for the KRAS PCR and EGFR kits?
FAQ ID -2293
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What voltage should be used to run my agarose gel with the GelPilot Agarose?
FAQ ID -2261
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Which mode should I use in the Rotor-Gene ScreenClust HRM Software, supervised or unsupervised?
FAQ ID -2202
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Does the atypical look of the lyophilized FlexiTube siRNA Premix have a negative impact on performance?
FAQ ID -2267
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Does the miScript Target Protector match the target sequence?
FAQ ID -2250
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Do the KRAS and EGFR PCR kits contain a passive reference dye?
FAQ ID -2295
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Do I have to order at least 4 FlexiTube siRNA Premixes?
FAQ ID -2266
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How do I store my FlexiTube siRNA Premix?
FAQ ID -2270
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How is ARMS technology superior to alternative methods such as dye terminator sequencing?
FAQ ID -2290
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Effects of low A260/A230 ratios in RNA preparations on downstream applications
FAQ ID -2248
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How can I check the success of the normalization procedure for the SeqTarget System?
FAQ ID -2245
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How do I search for all the miScript Target Protectors for one species?
FAQ ID -2255
浏览
Can PCR fragments be normalized for the SeqTarget System after purification with QIAquick?
FAQ ID -2240
浏览
Can PCR fragments be normalized for the SeqTarget System without a previous purification step?
FAQ ID -2246
浏览
Can I use a 384 well plate for the KRAS and EGFR PCR kits?
FAQ ID -2296
浏览
Are all 3 components of the SeqTarget System required or can a different Long Range PCR Kit and primers be used?
FAQ ID -2234
浏览
Can the miScript Precursor Assay differentiate between pri- and pre-miRNA?
FAQ ID -2211
浏览
Does ethanol from the PCR product purification influence the normalization procedure for the SeqTarget System?
FAQ ID -2244
浏览
What are EasyXtal 15-well Tools used for?
FAQ ID -2217
浏览
What are Principal Components analyzed in Rotor-Gene ScreenClust HRM Software?
FAQ ID -2200
浏览
Is the SeqTarget System compatible with standard-size PCRs?
FAQ ID -2241
浏览
What are the amplicon lengths of the KRAS and EGFR PCR kits?
FAQ ID -2289
浏览
What are clusters analyzed in the Rotor-Gene ScreenClust HRM Software?
FAQ ID -2201
浏览
What does QIAGEN use for a positive control in precursor detection experiments?
FAQ ID -2212
浏览
What controls are used in the KRAS and EGFR PCR kits to ensure reliable results?
FAQ ID -2291
浏览
What do I do if I cannot see amplification in the FAM channel in my KRAS and EGFR PCR kits?
FAQ ID -2297
浏览
What are the corresponding QIAGEN names for former Biotage instruments?
FAQ ID -2285
浏览
What buffer conditions give the best resolution for agarose gel electrophoresis?
FAQ ID -2256
浏览
What can be the reason for low DNA yields after normalization with the SeqTarget System?
FAQ ID -2239
浏览
How much buffer should be used for agarose gel electrophoresis?
FAQ ID -2257
浏览
How much DNA should be loaded per well of an agarose gel?
FAQ ID -2259
浏览
How should gels be cast with the GelPilot Agarose so that optimal resolution is achieved?
FAQ ID -2258
浏览
How long does it take to generate results using the KRAS and EGFR PCR kits?
FAQ ID -2299
浏览
How long should PCR fragments be for the SeqTarget System?
FAQ ID -2236
浏览
How many miScript Precursor Assays do you offer?
FAQ ID -2213
浏览
Is it possible to use lower siRNA concentrations with FlexiTube siRNA Premix?
FAQ ID -2264
浏览
Is one able to use FlexiTube siRNA Premix for transfection of primary adherent cell types?
FAQ ID -2268
浏览
Is one able to use FlexiTube siRNA Premix for transfection of primary suspension cell types?
FAQ ID -2269
浏览
If I am working with a difficult-to-transfect cell type or if I obtain only weak silencing effects what can I do?
FAQ ID -2265
浏览
Is it possible to normalize to more or less than 500 ng with the SeqTarget System if needed?
FAQ ID -2235
浏览
Is it possible to purify and normalize long range PCRs produced with PCR kits from other vendors for the SeqTarget System?
FAQ ID -2243
浏览
Is it possible to purify viral RNA using FastLane Cell RT-PCR Kits?
FAQ ID -1484
浏览
Why is the RT step with the QuantiFast RT Kits much shorter compared to QuantiTect RT Kits?
FAQ ID -1451
浏览
What applications are offered on the QIAcube?
FAQ ID -1403
浏览
Why is the activation time for HotStarTaq Plus Polymerase in the QuantiFast SYBR Green Kits different from that for QuantiFast Probe Kits?
FAQ ID -1449
浏览
Why is the storage time for QuantiFast PCR Kits shorter than that for QuantiTect PCR Kits?
FAQ ID -1446
浏览
Why is the reaction volume for QuantiFast PCR Kits lower than that for QuantiTect PCR Kits?
FAQ ID -1447
浏览
Why is the QuantiFast denaturation step different for PCR and RT-PCR runs in the two-step protocol for the ABI 7500 and other cyclers?
FAQ ID -1442
浏览
What is the minimum number of siRNAs per order? If I order 40 siRNAs, can I get those on 2 plates?
FAQ ID -1468
浏览
Can purification columns from other suppliers be processed on the QIAcube?
FAQ ID -1406
浏览
Can QIAcube protocol runs be interrupted, and subsequently be continued to completion?
FAQ ID -1414
浏览
Are run reports and/or log files available on the QIAcube?
FAQ ID -1412
浏览
Can I use more than 4 µl FastLane lysate in subsequent real-time RT-PCR?
FAQ ID -1480
浏览
Are FlexiPlate siRNAs available at a higher final concentration than 10 µM?
FAQ ID -1476
浏览
Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with QuantiFast Probe PCR Kits?
FAQ ID -1452
浏览
Can different amounts of siRNA be ordered on one FlexiPlate?
FAQ ID -1473
浏览
Can 2 µl reaction volumes be used with QuantiFast PCR Kits?
FAQ ID -1440
浏览
Are there any specific cleaning solutions recommended for the QIAcube?
FAQ ID -1416
浏览
Do the QuantiTect Primer Assays work with QuantiFast SYBR Green Kits?
FAQ ID -1439
浏览
Do special settings have to be used for QuantiFast PCR Kits on the Eppendorf Mastercycler ep realplex?
FAQ ID -1437
浏览
Do I need to buy special kits for the QIAcube?
FAQ ID -1402
浏览
Do I need to discard partially used QIAcube tip racks?
FAQ ID -1418
浏览
What information is required to reorder specific siRNAs from a siRNA Set in FlexiPlate format?
FAQ ID -1463
浏览
What are the turnaround times for FlexiPlate siRNA orders?
FAQ ID -1478
浏览
What are the dimensions and weight of the QIAcube?
FAQ ID -1407
浏览
How much time will be saved when switching from standard cycling to fast cycling with QuantiFast Kits?
FAQ ID -1438
浏览
How many reactions can I perform with the new QuantiFast Kits for real-time PCR?
FAQ ID -1425
浏览
If FlexiPlate siRNA was accidentally redissolved in siRNA Suspension Buffer instead of water, resulting in a 2x buffer, would it affect performance of the siRNAs?
FAQ ID -1477
浏览
How do you achieve fast cycling, yet still deliver the same performance in PCR as that achieved with standard cycling?
FAQ ID -1430
浏览
Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube?
FAQ ID -1415
浏览
Why is a 2-step (and not a 3-step) cycling protocol recommended for QuantiFast SYBR Green PCR Kits?
FAQ ID -1450
浏览
Why do QuantiFast SYBR Green PCR Kits require such a high primer concentration?
FAQ ID -1443
浏览
What QuantiFast Kit should be used on the Eppendorf Mastercycler ep realplex?
FAQ ID -1436
浏览
What is the detection limit of the QuantiFast Kits for real-time PCR?
FAQ ID -1432
浏览
Which kind of 96 and 384-well plates are available for FlexiPlate siRNAs?
FAQ ID -1459
浏览
Which cell lines have been tested with FastLane Cell RT-PCR Kits?
FAQ ID -1485
浏览
Can I adjust the ROX concentration in the QuantiFast master mix?
FAQ ID -1427
浏览
Can FlexiPlate siRNAs be ordered in plates other than the standard NUNC 96 well V bottom plates?
FAQ ID -1458
浏览
Can I follow the reaction setup for probe-based assays described in Appendix D of the FastLane Cell RT-PCR Handbook using 384-well plates?
FAQ ID -1482
浏览
Can fast cycling be carried out with QuantiFast Probe PCR Kits and FRET probes?
FAQ ID -1433
浏览
Can FlexiPlate siRNA be ordered in solution on dry-ice?
FAQ ID -1474
浏览
Can I program my own protocols for the QIAcube?
FAQ ID -1405
浏览
How can I load new protocols onto the QIAcube?
FAQ ID -1421
浏览
How can I get software updates for the QIAcube?
FAQ ID -1413
浏览
How do QuantiFast PCR Kits compare to QuantiTect PCR Kits for quantitative real-time PCR?
FAQ ID -1441
浏览
Do you offer trial-kit sizes for the new QuantiFast Kits?
FAQ ID -1429
浏览
How can I decontaminate the QIAcube system?
FAQ ID -1419
浏览
Can the QIAcube heater/shaker be used independently from protocol runs?
FAQ ID -1422
浏览
Can the QIAcube rotor adapters be autoclaved?
FAQ ID -1409
浏览
Can the QIAcube centrifuge be used independently from protocol runs?
FAQ ID -1423
浏览
Can QuantiFast PCR Kits be used on real-time PCR instruments without fast cycling options?
FAQ ID -1428
浏览
Can the QIAcube be connected to a laboratory information management system?
FAQ ID -1411
浏览
Can the QIAcube rotor and/or buckets be removed for cleaning?
FAQ ID -1410
浏览
Does the high primer concentration required by QuantiFast SYBR Green PCR Kits impair annealing specificity?
FAQ ID -1444
浏览
Does the master mix in the QuantiFast Kits contain dUTP to allow UNG treatments?
FAQ ID -1431
浏览
Can the QuantiFast Probe PCR +ROX Vial mastermix be stored long-term after adding ROX dye?
FAQ ID -1435
浏览
What are the Tms of PCR products obtained using the miScript System?
FAQ ID -1599
浏览
Do you have a protocol for cleanup of REPLI-g amplified DNA?
FAQ ID -1545
浏览
What can be done if the protein pellet does not solubilize completely using the AllPrep DNA/RNA/Protein Mini Kit?
FAQ ID -1580
浏览
What controls should be used for normalizing miRNA real-time PCR data obtained with the miScript System?
FAQ ID -1596
浏览
What is Q-Bond used in the QIAGEN Fast Cycling PCR and QuantiFast Kits?
FAQ ID -1554
浏览
Will QuantiTect Primer Assays work at an annealing temperature of 60ºC with QuantiFast SYBR Green PCR and RT-PCR Kits?
FAQ ID -1553
浏览
Which homogenization method do you recommend for tissues stored in Allprotect Tissue Reagent?
FAQ ID -1572
浏览
Can different incubation times be used for the 2 hour amplification step in the QuantiTect Whole Transcriptome protocol?
-8
浏览
Does residual Allprotect Tissue Reagent left behind on tissue samples interfere with DNA, RNA, and protein purification?
FAQ ID -1573
浏览
Is cDNA generated with the QuantiTect Whole Transcriptome Kit suitable for use in microarray analysis?
FAQ ID -1587
浏览
Do you have a protocol for purification of genomic DNA from cultured cells using the QIAamp DNA Micro Kit?
FAQ ID -1547
浏览
Do you have a protocol for concentration of RNA in 96-well plates?
FAQ ID -1546
浏览
Do you have a protocol for purification of total RNA from fatty tissues using QIAzol Lysis Reagent and MaXtract High Density?
FAQ ID -1550
浏览
Which real-time PCR kits are recommended downstream of the QuantiTect Whole Transcriptome Kit?
FAQ ID -1592
浏览
What primers are used in the reverse-transcription step of the QuantiTect Whole Transcriptome procedure?
FAQ ID -1584
浏览
What is the maximum amount of RNA that can be used for amplification with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1591
浏览
Can the AllPrep DNA/RNA/Protein Mini Kit be used with fibrous or lipid tissues?
FAQ ID -1579
浏览
What starting amount of RNA purified using an miRNeasy Kit should be used with the miScript System?
FAQ ID -1597
浏览
Can samples in Allprotect Tissue Reagent undergo repeated freeze-thaws without degradation of DNA, RNA, and protein?
FAQ ID -1575
浏览
Can the QuantiFast Probe PCR +ROX Vial Kit be used on the ABI PRISM 7900 and Applied Biosystems 7500 cyclers?
FAQ ID -1555
浏览
Can RNA purified from formalin-fixed, paraffin-embedded (FFPE) tissue be used with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1583
浏览
Does Buffer APP in the AllPrep DNA/RNA/Protein Mini Kit contain acetone?
FAQ ID -1576
浏览
Can tissue homogenized in Buffer RLT Plus instead of Buffer RLT be used with the AllPrep DNA/RNA/Protein Mini Kit?
FAQ ID -1577
浏览
Does cDNA amplified with the QuantiTect Whole Transcriptome Kit correspond to full-length RNA transcripts?
FAQ ID -1585
浏览
Can tissue incubated in Allprotect Tissue Reagent be used for morphological studies?
FAQ ID -1571
浏览
Can less than 10 ng RNA be used with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1590
浏览
Can cDNA prepared by any method be used as starting material in the ligation reaction of the QuantiTect Whole Transcriptome Kit?
FAQ ID -1589
浏览
Can Allprotect Tissue Reagent be used to stabilize RNA, DNA and proteins in cells, bacteria, yeast, and plants?
FAQ ID -1574
浏览
Can degraded RNA be used with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1586
浏览
How much cDNA is sufficient for real-time PCR detection of miRNA transcripts generated with the miScript System?
FAQ ID -1598
浏览
How many samples can be run in parallel with the Rotor-Gene Q real-time PCR instrument?
FAQ ID -1519
浏览
What is the advantage of EpiTect MethyLight PCR Kits over QuantiTect Probe PCR Kits for methylation studies?
FAQ ID -2010
浏览
What is the advantage of using the Type-it Microsatellite PCR Kit?
FAQ ID -2059
浏览
What is the advantage of using the Type-it Mutation Detect PCR Kit?
FAQ ID -2064
浏览
What is a good starting amount of template DNA for PCR with the EpiTect MSP Kit?
FAQ ID -2005
浏览
What is elution Buffer ATE of the QIAsymphony DNA Investigator Kit?
FAQ ID -2029
浏览
What is the maximum lifetime of the QIAGEN rotor for the Centrifuge 4 and 4K?
-16
浏览
What is the maximum DNA yield from forensic samples processed with the QIAsymphony SP?
FAQ ID -2028
浏览
What is the principle behind the DNA Polymerase in the EpiTect MSP Kit?
FAQ ID -2003
浏览
Is it possible to use different sample types in one run with the MagAttract 96 cador Pathogen Kit?
FAQ ID -2072
浏览
What is the purpose of the unmethylated and unconverted control DNA of the EpiTect PCR Control DNA Set?
FAQ ID -2007
浏览
Is magnetic particle carry-over in the eluates on the BioSprint 96 normal?
FAQ ID -2073
浏览
What happens when using EpiTect MethyLight Assays on only partially bisulfite converted DNA template?
FAQ ID -2009
浏览
Should sample lysate volumes be exactly 200 µl, 500 µl, or 1000 µl when loaded on the QIAsymphony SP?
FAQ ID -2031
浏览
What are the expected PCR results when using EpiTect Control DNA on untreated or bisulfite-converted DNA?
FAQ ID -2011
浏览
What are the features of the expression vector used for QIAgenes Insect/Mammalia?
FAQ ID -2050
浏览
Why do casework-sample protocols for the QIAsymphony DNA Investigator Kit start with different lysate volumes (200 µl, 500 µl, or 1000 µl)?
FAQ ID -2035
浏览
Why is CoralLoad included in the Type-it Mutation Detect, but not in the Type-it Microsatellite PCR Kit?
FAQ ID -2068
浏览
Why is there more than one QIAgene for my gene of interest?
FAQ ID -2044
浏览
Why do pQE-T7 vectors of the QIAgenes E. coli system have an additional UAG amber stop codon?
FAQ ID -2043
浏览
Why is the Type-it Mutation Detect PCR Kit recommended for preamplification of SNPs?
FAQ ID -2067
浏览
Will the optimized DNA sequence for QIAgenes be provided?
FAQ ID -2045
浏览
Why is maximum amplicon size for the Type-it Microsatellite PCR Kit limited to 500 bp?
FAQ ID -2060
浏览
Why is special Type-it Fast SNP Probe PCR chemistry required for TaqMan® SNP Genotyping?
FAQ ID -2057
浏览
What sample volumes can be processed with the QIAsymphony Virus/Bacteria Kits?
FAQ ID -2037
浏览
What sample types can be processed using the QIAsymphony Virus/Bacteria protocols?
FAQ ID -2036
浏览
Can data be analyzed on the Rotor-Gene Q while a run is in progress?
FAQ ID -2091
浏览
Are there two vectors for protein expression using QIAgenes in insect or mammalian cells?
FAQ ID -2048
浏览
Are there recommendations for sensitive detection of underrepresented fragments using the Type-it Mutation Detect PCR Kit?
FAQ ID -2066
浏览
Can other probe technologies besides TaqMan® be used with the Type-it Fast SNP Probe PCR Kit?
FAQ ID -2058
浏览
Is mitochondrial DNA isolated with the QIAsymphony DNA Investigator Kit on the QIAsymphony SP?
FAQ ID -2030
浏览
Is a passive internal reference dye, like ROX, required on the Rotor-Gene Q to obtain reproducible results?
FAQ ID -2079
浏览
Is it possible to edit the sample sheet in the Rotor-Gene Q software after a PCR run?
FAQ ID -2092
浏览
Is it possible to import a standard curve from a previous PCR run on the Rotor-Gene Q?
FAQ ID -2093
浏览
What are the ideal and the largest PCR amplicon sizes when using the EpiTect MSP Kit?
FAQ ID -2004
浏览
What kind of samples should be processed using the large-volume protocol with the QIAsymphony DNA Investigator Kit?
FAQ ID -2034
浏览
Which expression systems can be used with the QIAgenes Insect/Mammalia expression constructs?
FAQ ID -2049
浏览
Which QIAsymphony Virus/Bacteria Kit size should be used for each protocol?
FAQ ID -2038
浏览
What types of reaction vessels are required for use in the Rotor-Gene Q?
FAQ ID -2085
浏览
Which capillary sequencers can be used for detection of amplicons from the Type-it Microsatellite PCR Kits?
FAQ ID -2063
浏览
Can the EpiTect PCR Control DNAs be used for mouse and rat assays?
FAQ ID -2008
浏览
Can the EpiTect PCR Control DNAs also be used as standard in real-time methylation specific quantification?
FAQ ID -2006
浏览
Can the protocols for purification of small RNAs from cells in the appendices of the AllPrep DNA/RNA 96 Handbook also be used with tissue samples?
FAQ ID -2001
浏览
Can solid samples be processed with the QIAsymphony DNA Investigator Kit on the instrument?
FAQ ID -2027
浏览
Can QIAGEN rotors used with the centrifuges 4C and 4KC also be used with 4-16 and 4-16K models?
-15
浏览
Can the MagAttract 96 cador Pathogen Kit also be used on the KingFisher 96 or MagMAX Express 96 instruments?
FAQ ID -2074
浏览
Do all QIAsymphony DNA Investigator protocols use carrier RNA?
FAQ ID -2033
浏览
Can TopTaq Master Mix simply be stored at room temperature on the lab bench for up to 4 months?
FAQ ID -2052
浏览
Can the Type-it Mutation Detect PCR kit be used for multiplex PCR of fragments longer than 500 bp?
FAQ ID -2065
浏览
Can the Type-it Microsaltellite PCR Kit be used on cyclers with fast ramping rates?
FAQ ID -2062
浏览
Can 0.1 ml and 0.2 ml tubes used on the Rotor-Gene Q be labeled?
FAQ ID -2082
浏览
How many signal readings are taken during the real-time data acquisition on the Rotor-Gene Q?
FAQ ID -2083
浏览
Can membrane proteins be expressed with QIAgenes expression kits?
FAQ ID -2047
浏览
Can DNase digestion be performed on the RNeasy 96 plate during the RNA purification part of the Allprep DNA/RNA 96 procedure?
FAQ ID -2000
浏览
Does an internal control have to be included for each QIAsymphony Virus/Bacteria protocol run?
FAQ ID -2040
浏览
Do QIAsymphony Virus/Bacteria samples require pretreatment before they are loaded onto the instrument?
FAQ ID -2039
浏览
Does the centrifugal force have any effect on the kinetics of a PCR reaction on the Rotor-Gene Q?
FAQ ID -2081
浏览
How can a C-terminal 6xHis-tagged QIAgene be expressed?
FAQ ID -2041
浏览
How can full-length genes or individual domains of the QIAgenes Insect/Mammalia constructs be subcloned?
FAQ ID -2051
浏览
Does the Rotor-Gene Q software have the capacity to export data?
FAQ ID -2089
浏览
Can DNA yields be increased by prolonging incubation with proteinase K in Buffer ATL?
FAQ ID -2032
浏览
Can frozen samples be used with the MagAttract 96 cador Pathogen Kit?
FAQ ID -2070
浏览
How fast does the rotor spin during a run on the Rotor-Gene Q?
FAQ ID -2080
浏览
How is the log graph threshold value (Ct) for the Rotor-Gene Q determined?
FAQ ID -2088
浏览
How long can DNA converted with EpiTect Bisulfite Kits be stored before use in methylation specific PCR with the EpiTect MSP Kit?
FAQ ID -2002
浏览
How does the built-in QC control for complete bisulfite conversion of DNA work on PyroMark Q24?
FAQ ID -2095
浏览
How will running less than 96 samples with the MagAttract 96 cador Pathogen kit affect usage of plasticware?
FAQ ID -2071
浏览
If orders cannot be placed online, how can QIAgenes be ordered?
FAQ ID -2046
浏览
If new dyes or chemistries appear on the real-time PCR market, will Rotor-Gene Q be capable of running those dyes?
FAQ ID -2087
浏览
How long does a single run on the PyroMark Q24 take?
FAQ ID -2096
浏览
How much space does the PyroMark Q24 instrument need?
FAQ ID -2098
浏览
Does the Type-it Microsatellite PCR Kit always require an annealing temperature of 60°C, or can pre-optimized annealing temperatures for target-primer systems be used?
FAQ ID -2061
浏览
How accurate and reliable is PyroMark Q24 in mutation analysis?
FAQ ID -2094
浏览
Is there a charge for user training on the QIAsymphony SP System?
FAQ ID -1900
浏览
Is the QIAsymphony RNA Kit compatible with stabilized samples?
FAQ ID -1944
浏览
Is sample pretreatment required for purifying RNA on the QIAsymphony SP?
FAQ ID -1938
浏览
Is RNA co-purified with DNA using QIAsymphony DNA Tissue protocols?
FAQ ID -1920
浏览
What are the expected DNA yields from tissue processed on the QIAsymphony SP?
FAQ ID -1919
浏览
QuantiTect Primer Assays are bioinformatically validated, genomewide primer sets. What does “bioinformatically validated” mean?
FAQ ID -1982
浏览
What are the dimensions and weight of the QIAsymphony SP System?
FAQ ID -1901
浏览
What is the range of elution volumes on the QIAsymphony SP?
FAQ ID -1908
浏览
How many PCR reactions can be performed for different scales of miScript Primer Assays?
FAQ ID -1990
浏览
How many samples can be processed per kit or per reagent cartridge for RNA purification on the QIAsymphony SP?
FAQ ID -1940
浏览
How does QIAsymphony SP keep cellular samples in suspension prior to pipetting so that samples do not settle?
FAQ ID -1905
浏览
How efficient is DNA removal by a gDNA Eliminator 96 plate compared with DNase digestion on an RNeasy 96 plate?
FAQ ID -1994
浏览
How long can homogenates remain on the QIAsymphony SP without risk of RNA degradation?
FAQ ID -1941
浏览
How much sample can be used with the Allprep DNA/RNA 96 Kit?
FAQ ID -1997
浏览
How much sample can be used with the RNeasy Plus 96 Kit?
FAQ ID -1992
浏览
How much starting material can be used with the QIAsymphony DNA Mini Kit on the QIAsymphony SP?
FAQ ID -1917
浏览
What sample types can be used with the AllPrep DNA/RNA 96 Kit?
FAQ ID -1996
浏览
Which types and amounts of sample material can be processed for RNA extraction on the QIAsymphony SP?
FAQ ID -1937
浏览
What sample types can be used with the RNeasy Plus 96 Kit?
FAQ ID -1991
浏览
Will mitochondrial DNA also be isolated using the DNA Tissue protocols on the QIAsymphony SP?
FAQ ID -1921
浏览
What sample types should be processed with the High Content (HC) protocol for DNA extraction on the QIAsymphony SP?
FAQ ID -1918
浏览
Why does the Allprep DNA/RNA 96 Kit use Buffer RLT, whereas the AllPrep DNA/RNA Mini Kit uses Buffer RLT Plus?
FAQ ID -1999
浏览
Why is there no vacuum-only protocol for the RNeasy Plus 96 Kit?
FAQ ID -1993
浏览
What is the turn around time for custom miScript miRNA mimics, inhibitors and primer assays?
FAQ ID -1985
浏览
Is it possible to modify QIAsymphony protocols?
FAQ ID -1903
浏览
What are the starting volumes of homogenate for RNA extraction on the QIAsymphony SP?
FAQ ID -1939
浏览
What are the expected yields of total RNA isolated with the QIAsymphony SP?
FAQ ID -1942
浏览
What kind of molecules are miScript miRNA Inhibitors?
FAQ ID -1983
浏览
What sample input volumes can be used on the QIAsymphony SP?
FAQ ID -1907
浏览
What sample types can be processed using the tissue protocols for DNA extraction on the QIAsymphony SP?
FAQ ID -1915
浏览
What kind of molecules are miScript miRNA Mimics?
FAQ ID -1986
浏览
What RNA elution volumes are available with the QIAsymphony RNA Kit?
FAQ ID -1943
浏览
Do samples require pretreatment before loading onto the QIAsymphony SP for DNA extraction?
FAQ ID -1916
浏览
Do limiting primer concentrations need to be determined when using QuantiFast Multiplex PCR Kits?
FAQ ID -1976
浏览
Can uneven sample numbers be processed on the QIAsymphony SP module?
FAQ ID -1906
浏览
Does the QIAsymphony SP system generate run reports and/or log files?
FAQ ID -1911
浏览
Does HiPerFect work for the transfection of miScript miRNA mimics and inhibitors?
FAQ ID -1984
浏览
Do you have a protocol for the purification of archive-quality DNA from 0.5–20 mg paraffin-embedded tissue using Gentra Puregene Kits?
FAQ ID -1964
浏览
Do you have a protocol for the purification of archive-quality DNA from 1 ml of whole blood with the Gentra Puregene Tissue Kit?
FAQ ID -1953
浏览
Do you have a protocol for the purification of archive-quality DNA from 100 to 5 x 10e8 cultured cells using Gentra Puregene Kits?
FAQ ID -1960
浏览
Do you have a protocol for rapid purification of archive-quality DNA from up to 9 x 10e6 cells using Gentra Puregene Kits?
FAQ ID -1969
浏览
Can I program my own protocols for the QIAsymphony SP?
FAQ ID -1904
浏览
Are QuantiFast Multiplex PCR Kits available in trial sizes?
FAQ ID -1981
浏览
Are there any specific cleaning solutions recommended for the QIAsymphony SP?
FAQ ID -1913
浏览
Can 4-plex, real-time PCR be performed on the ABI PRISM 7000, 7700, or 7900 using the QuantiFast Multiplex PCR Kit?
FAQ ID -1978
浏览
Can the QIAsymphony RNA Kit be used to purify RNA from blood?
FAQ ID -1945
浏览
Can the QIAsymphony SP Module be connected to a laboratory information management system (LIMS)?
FAQ ID -1909
浏览
Can protocol runs on the QIAsymphony SP be interrupted and resumed?
FAQ ID -1912
浏览
Can the QuantiFast Multiplex PCR Kits be used on Roche LightCycler systems with TaqMan® probes?
FAQ ID -1979
浏览
Can the protocols for purification of small RNAs from cells in the appendices of the RNeasy Plus 96 Handbook also be used with tissue samples?
FAQ ID -1995
浏览
Do you have a protocol for the purification of archive-quality DNA from marine invertebrate tissue using Gentra Puregene Kits?
FAQ ID -1950
浏览
Do you have a protocol for the purification of archive-quality DNA from nematodes using Gentra Puregene Kits?
FAQ ID -1951
浏览
Do you have a protocol for the purification of archive-quality DNA from human dried blood using Gentra Puregene Kits?
FAQ ID -1959
浏览
Do you have a protocol for the purification of archive-quality DNA from Gram-negative bacteria using Gentra Puregene Kits?
FAQ ID -1975
浏览
Do you have a protocol for the purification of archive-quality DNA from Gram-positive bacteria using Gentra Puregene Kits?
FAQ ID -1946
浏览
Do you have a protocol for the purification of archive-quality DNA from hair roots using Gentra Puregene Kits?
FAQ ID -1947
浏览
Do you have a protocol for the purification of DNA from fruit flies?
FAQ ID -1970
浏览
Do you have a protocol for the purification of archive-quality DNA from tissue fixed in ethanol or formalin using Gentra Puregene Kits?
FAQ ID -1974
浏览
How can the QIAsymphony SP system be decontaminated?
FAQ ID -1914
浏览
Do you have a protocol for the purification of archive-quality DNA from umbilical cord blood using Gentra Puregene Kits?
FAQ ID -1958
浏览
Do you have a protocol for the purification of archive-quality DNA from buffy coat using Gentra Puregene Kits?
FAQ ID -1955
浏览
Do you have a protocol for the purification of archive-quality DNA from whole blood using Gentra Puregene Kits?
FAQ ID -1954
浏览
Do you have a protocol for the purification of archive-quality DNA from cerebrospinal fluid (CSF) using Gentra Puregene Kits?
FAQ ID -1967
浏览
Do you have a protocol for the purification of archive-quality DNA from Chlamydomonas using Gentra Puregene Kits?
FAQ ID -1968
浏览
Do you have a protocol for the purification of archive-quality DNA from blood smears using Gentra Puregene Kits?
FAQ ID -1948
浏览
Do you have a protocol for the purification of archive-quality DNA from fecal cells using Gentra Puregene Kits?
FAQ ID -1972
浏览
Do you have a protocol for the purification of archive-quality DNA from fish using Gentra Puregene Kits?
FAQ ID -1973
浏览
Do you have a protocol for the purification of archive-quality DNA from fungus using Gentra Puregene Kits?
FAQ ID -1971
浏览
Do you have a protocol for the purification of archive-quality DNA from buccal brushes using Gentra Puregene Kits?
FAQ ID -1965
浏览
Do you have a protocol for the purification of archive-quality DNA from Eimeria oocysts using Gentra Puregene Kits?
FAQ ID -1952
浏览
What effect does homogenization have on DNA yield and integrity when using AllPrep DNA/RNA Kits?
FAQ ID -1751
浏览
What is the fidelity of TopTaq DNA Polymerase?
FAQ ID -1739
浏览
Is TopTaq DNA Polymerase a hot-start polymerase?
FAQ ID -1748
浏览
Which nucleotide analogs can be used with TopTaq DNA Polymerase?
FAQ ID -1741
浏览
Why might Affymetrix GeneChip Mapping assays interfere with the REPLI-g FFPE kit?
FAQ ID -1755
浏览
What is the upper length of PCR products that can be amplified with TopTaq DNA Polymerase and Master Mix Kits?
FAQ ID -1738
浏览
What is the shelf-life of TopTaq DNA Polymerase and Master Mix?
FAQ ID -1743
浏览
What is the principle of the REPLI-g FFPE Kit?
FAQ ID -1753
浏览
What is the largest plasmid size that can be purified using the QuickLyse Miniprep Kit?
FAQ ID -1752
浏览
What is the minimum amount of starting material that can be used with the RNeasy Plus Micro and AllPrep DNA/RNA Micro Kit?
FAQ ID -1750
浏览
Can small miRNA-containing RNA fractions be separated from large RNAs using the miRNeasy FFPE Kit?
FAQ ID -1737
浏览
What is the recommended sample size for use with the REPLI-g FFPE Kit?
FAQ ID -1756
浏览
Can miRNA and other RNAs smaller than 200 nucleotides be isolated with the AllPrep DNA/RNA Micro Kit or RNeasy Plus Micro Kit?
FAQ ID -1749
浏览
Are there problems with the default threshold setting on the Applied Biosystems 7500 when using QuantiFast Probe PCR Kits?
FAQ ID -1702
浏览
Can TA/UA cloning be done with PCR products amplified with TopTaq DNA Polmyerase and TopTaq Master Mix Kits?
FAQ ID -1740
浏览
At which step in the REPLI-g FFPE protocol should PicoGreen quantification be done?
FAQ ID -1758
浏览
Have QuantiTect Primer Assays been tested with QuantiFast SYBR Green PCR Kits on the Mastercycler ep realplex?
FAQ ID -1714
浏览
How can foaming of Buffer RLT Plus lysates be avoided when using RNeasy Plus Micro and Mini Kits, or the Allprep DNA/RNA Mini Kit?
FAQ ID -1734
浏览
Do you have protocols for sample processing on the Autopure LS instrument?
FAQ ID -1759
浏览
Can the REPLI-g FFPE procedure restore DNA derived from FFPE tissue samples in the original order?
FAQ ID -1754
浏览
Can the REPLI-g FFPE Kit also be used for formalin-fixed, ethanol preserved samples?
FAQ ID -1760
浏览
Do you have a protocol for the isolation of total RNA from paraffin-embedded sections on the Biorobot M48?
FAQ ID -1733
浏览
Does TopTaq Stabilizer of the TopTaq DNA Polymerase and Master Mix Kits interfere with downstream applications?
FAQ ID -1744
浏览
Do you have a forensic post-PCR purification protocol to purify double-stranded DNA fragments from PCR reactions?
FAQ ID -1761
浏览
Do CoralLoad dyes supplied in various QIAGEN PCR Kits interfere with downstream applications?
FAQ ID -1745
浏览
Is FlexiTube GeneSolution available for other scales than the 1 nmol scale?
FAQ ID -1666
浏览
What buffer should be used to dilute cDNA made using the miScript Reverse Transcription Kit?
FAQ ID -1601
浏览
What is the binding capacity of the Ni-NTA Superflow Cartridges?
FAQ ID -1603
浏览
Is it necessary to clean up cDNA prepared with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1610
浏览
Is the master mix of QuantiFast Kits for real-time PCR aliquoted into several tubes to prevent cross-contamination?
FAQ ID -1697
浏览
Is the QuantiTect Whole Transcriptome Kit suitable for miRNA amplification?
FAQ ID -1609
浏览
Is sequence information for HP Validated siRNAs provided in the FlexiTube format?
FAQ ID -1665
浏览
Is it necessary to include a genomic DNA removal step with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1614
浏览
Which siRNA controls are available in FlexiTube format?
FAQ ID -1661
浏览
Where can I find background information and literature on Whole Genome Amplification with REPLI-g Kits?
FAQ ID -1690
浏览
Why is there DNA in the no-template (negative) control when using the QuantiTect Whole Transcriptome Kit?
FAQ ID -1620
浏览
What is the composition of Buffer PE?
FAQ ID -1691
浏览
What is the maximum volume of RNA in solution that can be used with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1616
浏览
Can the Ni-NTA Superflow Cartridges and/or Strep-Tactin Cartridges be connected in series?
FAQ ID -1606
浏览
Can the Ni-NTA and Strep-Tactin Superflow Cartridges be reused?
FAQ ID -1607
浏览
Can I store cDNA generated by the miScript System?
FAQ ID -1602
浏览
Can the QuantiTect Whole Transcriptome Kit be used for amplification of RNA from LCM samples?
FAQ ID -1612
浏览
Does the QuantiTect Whole Transcriptome Kit work with RNA purified from bacteria, yeast, or plants?
FAQ ID -1615
浏览
Can T-Script® enzyme of the QuantiTect Whole Transcriptome Kit be substituted by Quantiscript Reverse Transcriptase?
FAQ ID -1617
浏览
Can the QuantiTect Whole Transcriptome reaction be stopped after the reverse transcription step to proceed with the ligation and amplification steps at a later time?
FAQ ID -1618
浏览
Are FlexiTube siRNA and FlexiTube GeneSolution also available for rat siRNAs?
FAQ ID -1664
浏览
Can I still reorder siRNA that has been removed from the GeneGlobe data base?
FAQ ID -1668
浏览
Can amplification with the QuantiTect Whole Transcriptome Kit be extended to more than 8 hours to improve cDNA yields?
FAQ ID -1608
浏览
Can FlexiTube siRNA or FlexiTube GeneSolution products be ordered in solution?
FAQ ID -1667
浏览
Can I select HP Validated and HP GenomeWide siRNAs for FlexiTube?
FAQ ID -1658
浏览
Can cDNA prepared with the QuantiTect Whole Transcriptome Kit be reampliied using REPLI-g Kits?
FAQ ID -1611
浏览
How comparable is CoralLoad gel loading dye contained in various QIAGEN PCR Kits to Sigma Red?
FAQ ID -1644
浏览
How fast is the 6xHis-tagged protein purification process using Ni-NTA Superflow Cartridges?
FAQ ID -1605
浏览
How long is cDNA generated with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1613
浏览
How long can I archive cDNA generated with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1623
浏览
Have you observed co-amplification of genomic DNA from RNA templates used in the QuantiTect Whole Transcriptome Procedure?
FAQ ID -1619
浏览
Do I need to resuspend FlexiTube siRNAs in siRNA Resuspension buffer?
FAQ ID -1659
浏览
Do you have a protocol for purification of total RNA from plant latex?
FAQ ID -1694
浏览
Do I need to use the miScript Universal Primer for detection of mRNA?
FAQ ID -1600
浏览
Do you offer a QuantiFast Kit for one-step RT-PCR?
FAQ ID -1695
浏览
Do you have a protocol for purification of total RNA from plant tissue with the RNeasy Lipid Tissue Mini Kit?
FAQ ID -1693
浏览
How many samples can be processed with the QIAsymphony DNA Mini Kit and the Virus Blood 200 protocol?
FAQ ID -2159
浏览
How often can the Strep-Tactin Superflow Plus resin be reused?
FAQ ID -2169
浏览
If housekeeping and target genes differ significantly in abundance, will they both be amplified with equal efficiency using the Rotor-Gene Multiplex PCR Kit?
FAQ ID -2129
浏览
If Vapor-Lock is used to overlay PCR reactions, which volume should be entered as reaction volume in the Rotor-Gene Q Software?
FAQ ID -2150
浏览
Is it normal that the binding capacity of GST-resins decreases with flow rate?
FAQ ID -2174
浏览
Is it possible to purify a GST-tagged protein under denaturing conditions using Glutathione Superflow?
FAQ ID -2172
浏览
Why is EvaGreen instead of SYBR Green used as fluorescent dye in the Type-it HRM PCR Kit?
FAQ ID -2196
浏览
Why is the initial activation step different for Rotor-Gene Probe, SYBR Green and Multiplex Kits?
FAQ ID -2118
浏览
Why is a 2-step (and not a 3-step) cycling protocol recommended for Rotor-Gene SYBR Green Kits?
FAQ ID -2122
浏览
Why do I need normalization using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2198
浏览
Why is there an asterisk for my miScript Primer Assays?
FAQ ID -2176
浏览
Will Rotor-Gene Kits also work on the Rotor-Gene 6000 and 3000 cyclers?
FAQ ID -2121
浏览
Will Uracil-N-Glycosylase (UNG) completely remove contaminating amplicons when using QuantiTect +UNG Kits?
FAQ ID -2139
浏览
Will QuantiTect Primer Assays work with Rotor-Gene SYBR Green Kits using an annealing step at 60ºC?
FAQ ID -2124
浏览
What is miRNA*? Do I need to consider this in my profiling experiment?
FAQ ID -2179
浏览
What are the main differences between Rotor-Gene and QuantiTect or QuantiFast PCR Kits?
FAQ ID -2119
浏览
What are possible reasons for reduced DNA yields with REPLI-g Kits?
FAQ ID -2148
浏览
What is the residuals plot in the Rotor-Gene ScreenClust HRM Software?
FAQ ID -2199
浏览
What is the target nucleic acid for the Virus Blood 200 Protocol on the QIAsymphony?
FAQ ID -2158
浏览
What is the minimum number of assays that can be ordered on QuantiTect Primer Assay Plates?
FAQ ID -2109
浏览
What is the detection limit of the Rotor-Gene and QuantiFast Multiplex RT-PCR Kits?
FAQ ID -2144
浏览
What is the maximum number of targets that can be amplified per reaction with the QuantiFast Multiplex RT-PCR Kit?
FAQ ID -2145
浏览
How should DNA isolated on the QIAsymphony be stored?
FAQ ID -2165
浏览
How should samples be stored before purification using the Virus Blood 200 Protocol on the QIAsymphony?
FAQ ID -2157
浏览
How pure should the chloroform used with the RNeasy Microarray Tissue Mini Kit be?
FAQ ID -2193
浏览
Why should I use the RNeasy Microarray Tissue Mini Kit when purifying RNA for microarray analysis?
FAQ ID -2188
浏览
Which is the best condition for eluting GST-tagged proteins from Glutathione Superflow?
FAQ ID -2173
浏览
Which elution volumes can be selected using the QIAsymphony Virus Blood 200 protocol?
FAQ ID -2160
浏览
What sample volumes can be used with the Virus Blood 200 Protocol on the QIAsymphony?
FAQ ID -2156
浏览
Which plate formats are offered for QuantiTect Primer Assay Plates?
FAQ ID -2106
浏览
What species are QuantiTect Primer Assay Plates offered for?
FAQ ID -2110
浏览
Why are the denaturation and annealing/extension times for QuantiFast Multiplex RT-PCR Kits much shorter than those for QuantiFast Multiplex PCR Kits?
FAQ ID -2147
浏览
Which sample types can be processed using the QIAsymphony Virus Blood 200 protocol?
FAQ ID -2154
浏览
How many reactions can be performed per well of the QuantiTect Primer Assay Plates?
FAQ ID -2107
浏览
Can I use the RNeasy Microarray Tissue Mini Kit for all tissue types?
FAQ ID -2190
浏览
Can assays for 20 and 100 reactions be ordered on the same QuantiTect Primer Assay Plate?
FAQ ID -2108
浏览
Can Glutathione Superflow resin be reused?
FAQ ID -2171
浏览
Can Uracil-N-Glycosylase (UNG) be added to the QuantiTect PCR Master Mix and be stored for later use?
FAQ ID -2138
浏览
Can QuantiTect Primer Assay Plates be ordered offline via e-mail or fax?
FAQ ID -2112
浏览
Are there any changes in the procedure using the Strep-Tactin Superflow Plus resin with the higher capacity over the standard matrix?
FAQ ID -2168
浏览
Can Uracil-N-Glycosylase (UNG) be purchased separately, independent of QuantiTect PCR Kits?
FAQ ID -2134
浏览
Are probes other than TaqMan® probes, such as FRET probes, compatible with fast cycling with the Rotor-Gene Multiplex PCR Kit?
FAQ ID -2130
浏览
Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with the Rotor-Gene Multiplex PCR Kit?
FAQ ID -2131
浏览
Can the Rotor-Gene Multiplex RT-PCR Kit be used on other cyclers?
FAQ ID -2142
浏览
Can Uracil-N-Glycosylase (UNG) be used with a one-step RT-PCR kit, such as the QuantiTect Probe RT-PCR Kit?
FAQ ID -2135
浏览
Can RNA isolated with the RNeasy Microarray Tissue Mini Kit also be used in downstream applications other than microarray analysis?
FAQ ID -2191
浏览
Are Rotor-Gene Kits compatible with reaction setup using the QIAgility instrument?
FAQ ID -2116
浏览
Are QuantiTect PCR +UNG Kits available in bulk format (e.g., 25 ml master mix)?
FAQ ID -2136
浏览
Are the layouts for both 96- and 384-well QuantiTect Primer Assay Plates visible when ordering online?
FAQ ID -2111
浏览
Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with Rotor-Gene Probe Kits?
FAQ ID -2126
浏览
How does template quality influence the results with the Type-it HRM PCR Kit?
FAQ ID -2197
浏览
How does magnetic-particle carry-over in QIAsymphony eluates need to be handled?
FAQ ID -2166
浏览
Do you have protocols for software setup on various PCR platforms using QuantiFast Probe RT-PCR Kits?
FAQ ID -2105
浏览
How does the RNeasy Microarray Tissue Mini Kit prevent traces of phenol from negatively affecting the reverse-transcription step in microarray experiments?
FAQ ID -2189
浏览
How do Rotor-Gene Probe Kits compare with QuantiFast Probe Kits?
FAQ ID -2125
浏览
How many elution steps are recommended when using Glutathione Superflow resin?
FAQ ID -2175
浏览
How is the RNeasy Microarray Tissue Mini Kit different from the classic RNeasy Mini Kit?
FAQ ID -2192
浏览
Do you have a protocol for Rotor-Gene software setup for the Rotor-Gene SYBR Green PCR and RT-PCR Kits?
FAQ ID -2104
浏览
Does carrier RNA have to be added using the QIAsymphony Virus Blood 200 Protocol?
FAQ ID -2163
浏览
Does an internal control have to be used with the QIAsymphony Virus Blood 200 Protocol?
FAQ ID -2162
浏览
Can Vapor-Lock be used with samples processed on the QIAgility?
FAQ ID -2153
浏览
Do QuantiTect Primer Assays also work with Rotor-Gene SYBR Green PCR Kits?
FAQ ID -2123
浏览
Do the master mixes in Rotor-Gene Kits contain dUTP to allow UNG pretreatment?
FAQ ID -2117
浏览
Do limiting primer concentrations need to be determined when using the Rotor-Gene Multiplex PCR Kit?
FAQ ID -2128
浏览
What main input voltage is required for the QIAxcel Analyzer?
FAQ ID -1821
浏览
What is the minimum sample volume required for the QIAxcel System and QIAxcel Advanced?
FAQ ID -1852
浏览
Is there a quality difference between CompactPrep Plasmid Mega and Giga and the CompactPrep Plasmid Midi and Maxi Kits?
FAQ ID -1882
浏览
The Gel Cartridge is installed and all status indicators for the QIAxcel System are good but when clicking the "RUN" button, nothing happens. The green highlight goes down the Sequence?
FAQ ID -1850
浏览
Why does the copy function not apply relative to migration time and normalized area values after loading the DNA size marker table and adding the alignment marker peaks on the QIAxcel System?
FAQ ID -1832
浏览
What can be done if lower and upper alignment markers used with the QIAxcel System or QIAxcel Advanced are not aligned correctly?
FAQ ID -1834
浏览
Why are there no peaks in all channels during a run with the QIAxcel System or QIAxcel Advanced??
FAQ ID -1847
浏览
Why do DNA samples appear in only a few channels when the alignment marker is working using the QIAxcel System or QIAxcel Advanced?
FAQ ID -1844
浏览
Why does the Power LED not function with the QIAxcel or QIAxcel Advanced instrument plugged in and the main power switch turned on?
FAQ ID -1851
浏览
Can a QIAxcel DNA Fast Analysis Cartridge be run on a QIAxcel System?
FAQ ID -1828
浏览
Can customers use their own DNA size marker with the QIAxcel System and if yes, what concentration should be used?
FAQ ID -1841
浏览
Can data be saved at once with the QIAxcel System after analysis of all samples in a 96-well PCR plate?
FAQ ID -1861
浏览
How can a positive threshold be set when running the QIAxcel System?
FAQ ID -1843
浏览
How many intensity calibration runs can be performed per Gel Cartridge on the QIAxcel System?
FAQ ID -1827
浏览
How long will the intensity calibration procedure on the QIAxcel System take?
FAQ ID -1826
浏览
Why does an intensity calibration of the gel cartridge for the QIAxcel System and QIAxcel Advanced need to be performed?
FAQ ID -1824
浏览
Will the QIAxcel System run using a green Data Review Software Key?
FAQ ID -1853
浏览
Why is 'NO COM' displayed on the QIAxcel Instrument Panel although the power is on and all connections are made to the QIAxcel instrument and computer?
FAQ ID -1848
浏览
Why is the alignment marker for the 1.8 kb fragment not visible when using the 25 bp/1.8 kb DNA Size Marker together with the 15 bp/400 bp marker on the QIAxcel System and QIAxcel Advanced?
FAQ ID -1835
浏览
When running RNA samples using the QIAxcel System or QIAxcel Advanced, are there any special things to consider?
FAQ ID -1842
浏览
What to do if alignment marker and samples are migrating too slowly and the upper alignment marker does not appear on the gel- and/or electropherogram view with the QIAxcel System or QIAxcel Advanced?
FAQ ID -1833
浏览
Why are PCR bands relatively weak and "smearing" but the alignment marker bands are sharp when running samples on the QIAxcel System and QIAxcel Advanced?
FAQ ID -1837
浏览
When should an intensity calibration be re-performed on the QIAxcel System?
FAQ ID -1825
浏览
When should the N2 bottle used with the QIAxcel System be changed?
FAQ ID -1830
浏览
Can the QIAxcel software be loaded to another PC to view the data generated with the QIAxcel System?
FAQ ID -1855
浏览
Can the QIAxcel System or QIAxcel Advanced be connected to a network?
FAQ ID -1862
浏览
How can a clogged capillary channel be identified when running samples on the QIAxcel System or QIAxcel Advanced?
FAQ ID -1836
浏览
Can multiple QIAxcel Systems be run from one computer?
FAQ ID -1860
浏览
If a different DNA size and/or alignment marker is to be used with the QIAxcel System or QIAxcel Advanced, does the Gel Cartridge need to be recalibrated?
FAQ ID -1858
浏览
If a different method is used with the QIAxcel System or QIAxcel Advanced, does a new reference marker table need to be created?
FAQ ID -1857
浏览
How often should the alignment marker for the QIAxcel System be changed?
FAQ ID -1839
浏览
How often should the solutions in the Buffer Tray for the QIAxcel System or QIAxcel Advanced be replaced?
FAQ ID -1840
浏览
In the results table generated with the QIAxcel System, what does "normalized area" mean?
FAQ ID -1854
浏览
How can drying out of the gel cartridge tips used on the QIAxcel System and QIAxcel Advanced be prevented?
FAQ ID -1823
浏览
If the M500 method is used with the QIAxcel System, does a new DNA reference marker table have to be created?
FAQ ID -1856
浏览
How can any clogged capillaries be cleared for use with the QIAxcel System and QIAxcel Advanced?
FAQ ID -1838
浏览
What is the composition of buffer QBT?
FAQ ID -411
浏览
What is the composition of buffer QC?
FAQ ID -412
浏览
What is the composition of buffer P3?
FAQ ID -418
浏览
What is the composition of buffer QF?
FAQ ID -413
浏览
What is the composition of buffer TE?
FAQ ID -416
浏览
What is the composition of buffer QN?
FAQ ID -414
浏览
What is the composition of buffer OCD?
FAQ ID -423
浏览
What is the composition of buffer FWB2?
FAQ ID -417
浏览
What is the composition of buffer OBB?
FAQ ID -421
浏览
What is the composition of buffer OW2?
FAQ ID -419
浏览
What is the composition of buffer OL2?
FAQ ID -424
浏览
What is the composition of buffer OCL?
FAQ ID -422
浏览
Can RNAi experiments be performed in Drosophila?
FAQ ID -436
浏览
What is the size, charge and isoelectric point of the DHFR protein in the pQE vectors?
FAQ ID -470
浏览
Can the QIAamp MinElute Virus Kits be used to extract viral nucleic acid from cell culture supernatant?
FAQ ID -472
浏览
Has RNAi been successful using siRNA in Zebrafish and Xenopus?
FAQ ID -400
浏览
How is the RNeasy Lipid Tissue Mini Kit different from the RNeasy Mini Kit?
FAQ ID -468
浏览
How do I calculate the percentage of silencing with real-time RT-PCR for siRNA?
FAQ ID -498
浏览
Are Northern Blots sensitive enough to detect siRNA-induced gene silencing?
FAQ ID -403
浏览
Can QIAquick Kits be used to clean up RNA samples?
FAQ ID -490
浏览
Are HEPES, MOPS and PIPES buffers compatible with QIAGEN's PhosphoProtein Purification kit?
FAQ ID -482
浏览
Can Ni-NTA resins be used to purify protein with an internal His-tag?
FAQ ID -496
浏览
What is the composition of buffer STE?
FAQ ID -415
浏览
Can I use the RNeasy MinElute Cleanup Kit to clean up my in vitro transcription reaction?
FAQ ID -429
浏览
What is the composition of buffer OEB?
FAQ ID -420
浏览
Why are the centrifugation speeds for the QIAamp DNA Mini kit at 6000 x g? Can I spin at full speed?
FAQ ID -474
浏览
Which kit should be used to extract RNA from adipose tissue, brain, and other fatty animal tissues?
FAQ ID -467
浏览
Why is the pQE DNA provided in QIAexpress Kits blue in color?
FAQ ID -487
浏览
Why is carrier RNA used during the isolation of gDNA from microdissected samples with the QIAamp DNA Micro Kit?
FAQ ID -473
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What is the minimum elution volume when using the RNeasy MinElute Cleanup Kit?
FAQ ID -432
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What is the minimum elution volume when using the RNeasy Micro Kit?
FAQ ID -428
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What is the smallest sample size that can be used with RNeasy Mini Kits?
FAQ ID -485
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What is the maximum amount of starting material that can be processed with the RNeasy Micro Kit?
FAQ ID -426
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What detection reagents (reporter fluorophores) do you recommend for the LiquiChip System?
FAQ ID -480
浏览
What is a homogeneous LiquiChip assay?
FAQ ID -476
浏览
What are the excitation and emission wavelengths of the reporter and classification lasers of the LiquiChip Instrument?
FAQ ID -479
浏览
What is the binding capacity of the RNeasy Micro Kit column?
FAQ ID -427
浏览
What is the composition and size of the LiquiChip Beads?
FAQ ID -475
浏览
What is the binding capacity of the RNeasy MinElute Cleanup Kit column?
FAQ ID -430
浏览
Is dihydrofolate reductase (DHFR) immunogenic?
FAQ ID -471
浏览
What are some of the assays that are adaptable to the LiquiChip System?
FAQ ID -477
浏览
My QuantiTect Master Mix did not freeze at degrees. Is it still ok to use?
-20
浏览
Will REPLI-g work at high temperatures?
FAQ ID -656
浏览
What is the run time for the BioSprint 96 DNA Blood protocol on the BioSprint 96 instrument?
FAQ ID -622
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The Multiflow Dispense Head on my BioRobot is not dispensing the correct amount of liquid. What can I do?
FAQ ID -652
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What is the smallest amount of DNA I can put into a 50 ul REPLI-g reaction?
FAQ ID -663
浏览
What is the shelf-life of my QIAGEN Kit?
FAQ ID -651
浏览
What is the recommended incubation time to stabilize tissue RNA in RNAprotect?
FAQ ID -647
浏览
What do you mean by “Quantification of locus representation” in REPLI-g WGA product?
FAQ ID -698
浏览
What is the average amount of DNA and RNA present in 1 ml normal serum?
FAQ ID -635
浏览
What is REPLI-g whole genome amplification?
FAQ ID -654
浏览
What is a QIAshredder? Is it sufficient for complete disruption and homogenization of my tissue sample?
FAQ ID -631
浏览
What do you recommend for the cleanup of genomic DNA (gDNA)?
FAQ ID -618
浏览
What is the expected yield of genomic DNA isolated from bacteria with the DNeasy Blood & Tissue or QIAamp DNA Mini Kit?
FAQ ID -632
浏览
What is the biggest protein that can be synthesized with the EasyXpress Protein Synthesis System?
FAQ ID -601
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What is the length of the MDA whole genome amplification product?
FAQ ID -690
浏览
What 96-well plates do you recommend for use with the LiquiChip Workstation?
FAQ ID -630
浏览
Is it possible to perform laser microdissection with RNAprotect-stabilized tissue?
FAQ ID -608
浏览
Is it possible to synthesize membrane proteins using the EasyXpress Protein Synthesis Kit?
FAQ ID -602
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What are Elution Microtube adapters and Microplate adapters used in the DirectPrep 96 Miniprep Kit, and how can I order them?
FAQ ID -646
浏览
What are the differences between MDA and DOP/PEP methods of Whole Genome Amplification?
FAQ ID -665
浏览
What are the advantages of REPLI-g over conventional DNA sample processing and amplification methods?
FAQ ID -686
浏览
Why do I get amplification in a negative control DNA tube using the REPLI-g Kit for WGA?
FAQ ID -675
浏览
Why does the QIAamp DNA Mini Tissue Protocol require both ATL and AL buffer, while the Blood Protocol only uses AL?
FAQ ID -633
浏览
Why do I sometimes get light blue colonies when using the QIAGEN PCR Cloning Kit?
FAQ ID -603
浏览
Why did you choose MAPK1 as a positive control in the QIAGEN RNAi Human/Mouse Control Kit?
FAQ ID -604
浏览
Why do you recommend using Triton X for the purification of 6xHis-tagged protein?
-100
浏览
Will the PhosphoSerine antibody recognize Serine residues if the neighboring residues are Leucine or Isoleucine?
FAQ ID -644
浏览
Will the QIAquick PCR Purification Kit remove sufficient SYBR Green from real-time PCR reactions to allow sequencing?
FAQ ID -637
浏览
Will TA cloning work with REPLI-g WGA product?
FAQ ID -672
浏览
Will DNA from chromosomes collected in TE with a drop of mineral oil work with REPLI-g amplification?
FAQ ID -664
浏览
What is the stability of the REPLI-g MDA product?
FAQ ID -693
浏览
When delivering liquid to a 96-well plate using the MultiFlow Dispenser Head on my BioRobot , one or more rows in the plate contains less liquid per well than specified by the protocol. What is going wrong?
FAQ ID -653
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Where should I add a dye-label modification on the siRNA?
FAQ ID -648
浏览
What types of materials from environmental samples could interfere with WGA?
FAQ ID -666
浏览
What size is the smallest protein that can be synthesized with the EasyXpress Protein Synthesis Kit?
FAQ ID -600
浏览
Can I stabilize RNA in bacteria grown on solid media or substrate using the RNAprotect Bacteria Reagent?
FAQ ID -615
浏览
Can I run less than 15 samples on the BioSprint 15 instrument?
FAQ ID -621
浏览
Can I re-amplify whole genome amplification product?
FAQ ID -670
浏览
Can I use another lysis buffer than the one supplied in the PhophoProtein Purification Kit?
FAQ ID -645
浏览
Can the QIAshredder Maxi Spin Columns from the DNeasy Plant Maxi Kit be used for RNeasy samples?
FAQ ID -616
浏览
Do you have an off-the shelf diagnostics kit for WGA?
FAQ ID -678
浏览
Can the RNase-Free DNase Set be used for DNase digestions of RNA in solution?
FAQ ID -619
浏览
Can the computer for the LiquiChip Workstation be networked?
FAQ ID -629
浏览
Are there components in biological samples that can inhibit the REPLI-g reaction?
FAQ ID -667
浏览
Are the BioSprint 15/96 instruments stand-alone workstations?
FAQ ID -620
浏览
Are samples that are contaminated with high molecular weight DNA (e.g. from a paramedic or police officer) a problem with REPLI-g amplification?
FAQ ID -683
浏览
Are QIAGEN EZ Competent Cells of the QIAGEN PCR Cloning Kit dam+ or dam-?
FAQ ID -638
浏览
Can BAC DNA prepared with the R.E.A.L. Prep 96 Plasmid Kit be used for high-throughput sequencing?
FAQ ID -643
浏览
Can I do whole genome amplification from brain tissue using REPLI-g?
FAQ ID -669
浏览
Can FTA paper for dried blood spots be used with REPLI-g?
FAQ ID -685
浏览
Can I do whole genome amplification from mitochondrial DNA using REPLI-g?
FAQ ID -668
浏览
Can I amplify from plasma or serum samples using the REPLI-g Mini/Midi kits?
FAQ ID -671
浏览
How can I minimize degradation of RNA from my pancreas sample?
FAQ ID -624
浏览
How can I quantify the amount of REPLI-g DNA I have amplified?
FAQ ID -694
浏览
How should I store the REPLI-g amplification product?
FAQ ID -692
浏览
Is 2 kb the minimal gDNA fragment size for REPLI-g Whole Genome Amplification?
FAQ ID -682
浏览
How long can I store bacterial culture stabilized in RNAprotect Bacteria Reagent?
FAQ ID -614
浏览
Do I need to clean up the REPLI-g amplification product for downstream analysis?
FAQ ID -691
浏览
Does REPLI-g technology for whole genome amplification work with paraffin embedded samples?
FAQ ID -673
浏览
Do you have a kit for RNA isolation from any kind of sample type?
FAQ ID -627
浏览
How can I improve my RNA yield from liver sample when using RNeasy Kits?
FAQ ID -623
浏览
How can I determine the quality of my REPLI-g amplified products?
FAQ ID -695
浏览
Do you know of anything in the buffers/DNA sample that can cause bias in REPLI-g WGA?
FAQ ID -684
浏览
What is the composition of the QIAGEN 10x PCR Buffer in Taq- and HotStarTaq DNA Polymerase Kits?
FAQ ID -606
浏览
Is it possible to use less than 1 mg of protein with the PhosphoProtein Purification Kit?
FAQ ID -599
浏览
I want to start with gene silencing (RNAi) experiments. Do you have informational literature?
FAQ ID -580
浏览
What can I do when the DNA pellet prepared with QIAGEN Plasmid Kits has been overdried?
FAQ ID -572
浏览
What is a quenched FRET assay?
FAQ ID -542
浏览
Is it possible to use a QIAzol lysate for the isolation of RNA from tissue on the BioRobot EZ1?
FAQ ID -573
浏览
What do you recommend for isolating RNA from more than 100 mg of plant tissue?
FAQ ID -570
浏览
What happens if I spin my lysate on the RNeasy Spin Columns at maximum speed?
FAQ ID -514
浏览
What has to be done to a RNA sample before loading it onto an Agilent Bioanalyzer?
FAQ ID -528
浏览
How should I dissolve the lyophilized Anti-His and Tag Antibodies?
-100
浏览
Is it possible to isolate single stranded DNA (ssDNA) with the QIAEX II Kit from agarose or polyacrylamide gels?
FAQ ID -576
浏览
Is it possible to perform a UNG treatment when using QuantiTect kits?
FAQ ID -564
浏览
Is it possible to isolate DNA from bone marrow with the QIAamp DNA Blood Kits?
FAQ ID -563
浏览
Is it possible to clean up a methylation reaction containing bisulfite with QIAquick Cleanup Kits?
FAQ ID -519
浏览
Is it possible to isolate both RNA and recombinant 6xHis-tagged protein from the same sample?
FAQ ID -532
浏览
Why are samples centrifuged at 4°C after the addition of chloroform when using RNeasy Lipid Tissue Kits?
FAQ ID -533
浏览
Why are my realtime PCR amplification plots hook-shaped?
FAQ ID -587
浏览
How was it determined that the PhosphoSerine Antibody will bind phosphoserines only?
FAQ ID -579
浏览
If pQE40 is used to express a fusion protein with DHFR, can the DHFR be cleaved afterwards?
FAQ ID -513
浏览
How should I store cDNA produced using Omniscript Reverse Transcriptase?
FAQ ID -561
浏览
Which chloroform should I use for the RNeasy Lipid Tissue Kit?
FAQ ID -516
浏览
Will pancreas tissue swim on top of RNAprotect?
FAQ ID -536
浏览
Will the 2x QIAGEN Multiplex PCR Master Mix freeze at -20°C?
FAQ ID - 525
浏览
Why does my realtime PCR assay quality decrease over time?
FAQ ID -589
浏览
Why do replicates in real-time PCR have different plateau heights?
FAQ ID -539
浏览
What is the longest fragment that was cloned with the pQE UA Cloning Vector?
-30
浏览
What is the difference between BSA-free and BSA-containing RGS-His Antibody?
FAQ ID -569
浏览
What is the composition of Buffer ER? Is it available separately?
FAQ ID -571
浏览
What is the composition of the siRNA resuspension & annealing buffer?
FAQ ID -522
浏览
When using QIAzol Lysis Reagent for RNA extraction, I see a pinkish colored aqueous phase! Is it OK?
FAQ ID -534
浏览
Where can I find the public Smith-Waterman homology search tool that you refer to on your siRNA online design page?
FAQ ID -562
浏览
What reaction volume should I use with QuantiTect Kits?
FAQ ID -551
浏览
What is Tissue-Tek O.C.T., and what is it used for?
FAQ ID -530
浏览
What makes QIAGEN's 10x Taq and HotStarTaq DNA Polymerase PCR buffer superior?
FAQ ID -566
浏览
How many template copies can I detect using QuantiTect Kits in real-time PCR?
FAQ ID -543
浏览
Can I use RNAiFect for co-transfection of plasmid DNA and siRNA?
FAQ ID -515
浏览
Does DEPC harm RNA?
FAQ ID -529
浏览
Does endogenously expressed DAPase in human cells degrade proteins that are expressed in cell culture?
FAQ ID -527
浏览
Can Taq DNA Polymerase use RNA as a template, and generate false positives in "no-RT" controls?
FAQ ID -523
浏览
Can the MAPK1 control siRNA be used for rat cells?
FAQ ID -547
浏览
Are the columns of the MinElute Reaction Cleanup-, Gel Extraction-, and PCR Purification Kit identical?
FAQ ID -581
浏览
Are the columns of the QIAquick PCR Purification-, Gel Extraction-, and Nucleotide Removal Kit interchangeable?
FAQ ID -577
浏览
After using your U/A PCR Cloning Kit I can detect colonies, which are light blue, and not really white. Why is that?
FAQ ID -512
浏览
Can I make a master mix with QuantiProbes, primers, and all other reaction components and store it for later use?
FAQ ID -584
浏览
Can I shorten the activation time for the HotStarTaq DNA Polymerase?
FAQ ID -565
浏览
Can I homogenize samples in QIAzol using the QIAshredder?
FAQ ID -501
浏览
Do you offer QIAshredder spin columns for the RNeasy Midi/Maxi Kit format? If not, what is the alternative?
FAQ ID -560
浏览
How can I avoid primer-dimer formation during PCR amplification?
FAQ ID -544
浏览
Does QIAGEN sell 6xHis-tagged proteins besides the 6xHis Protein Ladder?
FAQ ID -594
浏览
Do you provide control proteins for use with the PhosphoProtein Purification Kit?
FAQ ID -596
浏览
How long should the amplicon be if I am using QuantiTect SYBR Green detection?
FAQ ID -553
浏览
How is an antibody epitope determined?
FAQ ID -593
浏览
Do I need to calibrate my real-time cycler if I want to use Yakima Yellow?
FAQ ID -540
浏览
How can I tell if I have primer-dimers in my PCR reaction?
FAQ ID -552
浏览
Do we recommend pooling siRNA sequences?
FAQ ID -517
浏览
Do I have to remove the oil from my PCR reaction before using the QIAquick or MinElute PCR Purification Kit?
FAQ ID -575
浏览
Does the His-tag interfere with the purification procedure of the QIAGEN PhosphoProtein Purification Kit?
FAQ ID -598
浏览
Does the use of MAPK1 as a control in siRNA transfection experiments have secondary effects on the cell culture?
FAQ ID -548
浏览
Do you have a protocol to isolate RNA from blood and bone marrow using the BioRobot EZ1?
FAQ ID -567
浏览
Do you have stability data for genomic DNA isolated with the QIAamp DNA Blood Mini Kit?
FAQ ID -518
浏览
Do you have a protocol for the removal of endotoxins from already purified plasmid DNA?
FAQ ID -500
浏览
Do you have a protocol for isolating RNA from yeast using RNeasy?
FAQ ID -535
浏览
Do you have any recommendations for using the PosphoProtein Kit with starting material other than cells?
FAQ ID -597
浏览
Do you have a protocol for isolation of genomic DNA from saliva using the DNeasy Blood & Tissue Kit?
FAQ ID -931
浏览
Do you have a protocol for the isolation of DNA from epithelial cells mixed with sperm cells?
FAQ ID -926
浏览
Do you have a protocol for the purification of DNA from rodent tail using the BioSprint System?
FAQ ID -979
浏览
Do you have a protocol for the isolation of DNA from tofu?
FAQ ID -932
浏览
Do you have a protocol for Streptokinase treatment of clotted blood?
FAQ ID -912
浏览
Do you have a protocol for purification of DNA from clotted blood using the FlexiGene DNA Kit?
FAQ ID -973
浏览
Do you have a protocol for the isolation of RNA from leukocytes in milk?
FAQ ID -952
浏览
Do you have a protocol for the isolation of total nucleic acids from animal and human tissues on the BioRobot EZ1?
FAQ ID -975
浏览
Do you have a protocol for the isolation of total nucleic acids from animal and human cells on the BioRobot EZ1?
FAQ ID -974
浏览
Do you have a protocol for the isolation of total RNA from buccal swabs?
FAQ ID -956
浏览
Do you have a protocol for the isolation of viral DNA from stool?
FAQ ID -929
浏览
Do you have a protocol for the isolation of total RNA from plant tissue using QIAGEN-tips?
FAQ ID -949
浏览
Do you have a protocol for the isolation of total RNA from paraffin-embedded sections on the BioRobot EZ1?
FAQ ID -976
浏览
Do you have a protocol for the isolation of total RNA from plants using the RNeasy 96 Kit?
FAQ ID -951
浏览
Do you have a protocol for the isolation of DNA from soft tissues using the TissueLyser?
FAQ ID -924
浏览
Do you have a protocol for the isolation of genomic DNA from sperm?
FAQ ID -909
浏览
Do you have a protocol for the isolation of DNA from formalin-preserved stool samples?
FAQ ID -922
浏览
Do you have a protocol for the isolation of DNA from fungi using the BioRobot EZ1?
FAQ ID -992
浏览
Do you have a protocol for the isolation of genomic DNA from fungi?
FAQ ID -911
浏览
Do you have a protocol for the isolation of genomic DNA and/or proteins from fatty tissue treated with QIAzol?
FAQ ID -955
浏览
Do you have a protocol for the isolation of genomic DNA from frozen clotted blood?
FAQ ID -900. Test
浏览
Do you have a protocol for the preparation of RNAprotect preserved tissues for pathological studies?
FAQ ID -950
浏览
Do you have a protocol for the purification of DNA from cells using the BioSprint System?
FAQ ID -977
浏览
Do you have a protocol for the purification of DNA from dried blood spots using the BioSprint System?
FAQ ID -982
浏览
Do you have a protocol for isolation of genomic and plasmid DNA from cell cultures using QIAGEN Genomic-tips?
FAQ ID -902
浏览
Do you have a protocol for transfection of adherent cells with low concentrations of siRNA using RNAiFect Transfection Reagent?
FAQ ID -970
浏览
Do you have a protocol for the purification of DNA from buccal swabs using the BioSprint System?
FAQ ID -983
浏览
Do you have a protocol for GMO testing of food samples?
FAQ ID -921
浏览
Do you have a protocol for isolation of bacterial DNA from soil?
FAQ ID -923
浏览
Do you have a protocol for isolation of genomic and viral DNA from lymphocytes using QIAGEN Genomic-tips?
FAQ ID -901
浏览
Do you have a protocol for isolation of genomic DNA from saliva and mouthwash?
FAQ ID -917
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Do you have a protocol for isolation of genomic DNA from dried blood spots using the QIAamp 96 DNA Blood Kit?
FAQ ID -919
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