NGS library quantification

Next-generation sequencing library quantification is an essential step for using your flow cells at full efficiency. Evidence shows that overloading or underloading an NGS library following inaccurate library quantification adversely affects data output and quality. Using digital PCR to determine the absolute concentration of an NGS library pool can be greatly beneficial for obtaining optimal yield and reducing cost per sample.

Benefits of using nanoplate digital PCR for NGS library quantification

Absolute quantification of amplifiable library fragments without amplification bias and without standard bias with results in 2 hours, suitable for routine testing
High reproducibility and superior uniformity for library pooling with coverage of all Illumina library types with one assay

Webinar: NGS library quantification using nanoplate digital PCR

Next-generation sequencing can be a long, expensive process if the NGS flow cells are used at less than full capacity. The most common cause of loading problems is poor library quantification. In this webinar, learn how to use digital PCR to accurately measure NGS libraries, regardless of their average fragment length and composition.

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