Quantity: In there enough material to assay?
Accurate sample quantification is the first step toward meaningful results by ensuring that accidental estimation errors do not disrupt the downstream assays
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Purity: Does the sample contain contaminants?
Assessing sample purity alerts you of any contamination or impurities that may interfere with the downstream application
Size: Is my sample degraded? Does it have the right size distribution?
Assessing nucleic acid size distribution gives insights into sample integrity and helps identify poor-quality or degraded samples not suitable for analysis
Sequence: Is this the right genotype?
Sequencing is the ultimate validation step that ensures you are working with the correct sample and gives full credit to your results interpretation