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  4. Bench Guide
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Next Guidelines for degenerate primer design and use
Previous PCR primer design
    • Guidelines for PCR
    • Types of PCR
      • Multiplex PCR
      • Long-range PCR
      • Single-cell PCR
      • Fast-cycling PCR
      • Methylation-specific PCR (MSP)
      • Hot start PCR
      • High-fidelity PCR
      • RAPD: Rapid amplified polymorphic DNA analysis
      • RACE: Rapid amplification of cDNA ends
      • In situ PCR
      • Differential display PCR
    • PCR primer design
    • PCR conditions
    • Guidelines for degenerate primer design and use
      • Primer sequence:
      • Primer concentration:
    • Amplification of long PCR products
    • Enzymes used in PCR
    • PCR cycling
    • Commonly used terms in PCR
    • PCR quantification
      • What is absolute quantification?
      • What is relative quantification?
    • Determining amplification efficiencies
      • Different amplification efficiencies
      • Comparable amplification efficiencies
      • Comparative method or ΔΔCT method of relative quantification
    • Real time PCR
    • Guidelines for RT-PCR
      • Two-step and one-step RT-PCR
      • Choice of RT primers
      • Conditions for two-step RT-PCR
      • Conditions for one-step RT-PCR
      • Enzymes used in RT-PCR
      • Multiplex PCR and RT-PCR
    • DNA contamination
    • Fundamentals of digital PCR
      • What is digital PCR?
      • How does digital PCR work?
      • Digital PCR workflow in action
      • Digital PCR applications
      • Benefits and limitations of digital PCR
      • Select publications using digital PCR
    • dPCR vs qPCR vs end-point PCR
      • What is dPCR?
      • What is end-point PCR?
      • What is qPCR?
      • RT PCR vs qPCR
      • Comparison of digital PCR vs. qPCR
      • How to switch from qPCR to dPCR
    • Comparison of digital PCR methods
    • Endogenous reference genes
    • PCR controls
      • No-template control
      • Positive control
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      • Internal controls
    • References
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