Accurate NGS library quantification using nanoplate digital PCR

Accurate quantification of next-generation sequencing (NGS) libraries is a prerequisite for optimal yield on Illumina NGS platforms. Absolute quantification of target molecules by digital PCR enables more accurate measurement of these libraries, resulting in efficient use of NGS flow cells.

In this webinar, we will introduce the QIAcuity dPCR workflow for quantifying Illumina NGS libraries using a library-specific primer assay in combination with the QIAcuity EG PCR Kit. We will showcase how to use the dPCR system to accurately quantify NGS libraries regardless of their average fragment length and composition.

Key learning objectives:

How to set up a dPCR reaction for NGS library quantification
What level of performance can you expect from dPCR for NGS library quantification
How quantification with the QIAcuity compares to standard methods

About the speaker

Dr. Ronny Kellner, R&D Senior Scientist, dPCR

QIAGEN

Dr. Ronny Kellner is an R&D Senior Scientist working on digital PCR assay development at QIAGEN, Hilden, Germany. He completed his Ph.D. in molecular plant-pathogen interaction at the MPI for Terrestrial Microbiology in Marburg and the Ruhr-University of Bochum, Germany. After several international projects in Germany and the UK focusing on microbiology, molecular biology and NGS data analysis, he joined QIAGEN in August 2020. Since then, he has been developing the digital PCR assay portfolio, including applications for microbial pathogen detection and NGS library quantification.