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Preamplification Kits

μ •ν™•ν•˜κ³  쒅합적인 λ°œν˜„ 뢄석

miRNA μ •λŸ‰ν™” μˆ˜ν–‰ μ‹œ, 적은 μ–‘μ˜ RNAκ°€ ν¬ν•¨λœ μƒ˜ν”Œλ‘œ μ‹œμž‘ν•˜λ©΄ μ‹€ν—˜μ˜ 성곡 여뢀에 영ν–₯을 λ―ΈμΉ  수 μžˆμŠ΅λ‹ˆλ‹€. QIAGEN의 RNA 사전 증폭 ν‚€νŠΈλŠ” 이에 λŒ€ν•œ 해결책을 μ œμ‹œν•©λ‹ˆλ‹€. 이 ν‚€νŠΈλŠ” real-time PCR을 μ‚¬μš©ν•˜μ—¬ μ •λŸ‰ν™” 및 ν”„λ‘œνŒŒμΌλ§ μ‹œ 편ν–₯λ˜μ§€ μ•Šκ³  μž¬ν˜„ κ°€λŠ₯ν•œ κ²°κ³Όλ₯Ό 얻을 수 μžˆλ„λ‘ ν•©λ‹ˆλ‹€. 이 κ°•λ ₯ν•œ μ ˆμ°¨μ—μ„œλŠ” miRNAκ°€ ν¬ν•¨λœ 총 RNAλ₯Ό cDNA ν•©μ„±μ˜ μ‹œμž‘ 물질둜 μ‚¬μš©ν•˜λ©°, μž‘μ€ RNAλ₯Ό λ³„λ„λ‘œ 농좕할 ν•„μš”κ°€ μ—†μŠ΅λ‹ˆλ‹€. λ˜ν•œ QIAGEN의 사전 증폭 ν‚€νŠΈλ‘œλŠ” μ΅œμ†Œ 10ng의 총 RNA둜 μ •ν™•ν•˜κ³  쒅합적인 λ°œν˜„ 뢄석을 μˆ˜ν–‰ν•  수 μžˆμŠ΅λ‹ˆλ‹€.

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