LNA Resource Center
Unlock more insights with LNA
The unique characteristics of LNA make it a powerful tool, not only for miRNA research but also for detection of low-abundance, short or highly similar targets in a number of applications. Our LNA resource center provides downloadable research papers, posters and videos containing useful information to help you get the best results from your LNA experiments.
LNA qPCR with the miRCURY LNA miRNA PCR System
Products:
- miRCURY LNA miRNA miRNome PCR Panels
- miRCURY LNA miRNA Focus PCR Panels
- miRCURY LNA miRNA Custom PCR Panels
- miRCURY LNA miRNA PCR Starter Kit
- miRCURY LNA RT Kit
- miRCURY LNA SYBR Green PCR Kits
- miRCURY LNA miRNA PCR Assays
- miRCURY LNA miRNA Custom PCR Assays
Resources:
- miRCURY LNA miRNA PCR – Exosomes, Serum/Plasma and Other Biofluid Samples Handbook
- miRCURY LNA miRNA PCR Handbook
- miRCURY LNA miRNA PCR System Product Profile
- Explore the RNA Universe! Poster
- miRCURY LNA miRNA PCR Assays and PCR Panels Quick-Start Protocol
- miRCURY LNA RT Kit Quick-Start Protocol
Publications:
- Park, J. et al. (2018) Expression profile of microRNAs following bone marrow‑derived mesenchymal stem cell treatment in lipopolysaccharide‑induced acute lung injury. Experimental and Therapeutic Medicine.
- Joris, V. et al. (2018) MicroRNA-199a-3p and MicroRNA-199a-5p take Pprt to a redundant network of regulation of the NOS (NO Synthase)/NO pathway in the endothelium. Arterioscler Thromb Vasc Biol.
- Catapano, F. et al. (2018). Downregulation of miRNA-29, -23 and -21 in urine of Duchenne muscular dystrophy patients. Future Medicine.
Koutsioumpa, M. et al. (2018) MKAD-21 suppresses the oncogenic activity of the miR-21/PPP2R2A/ERK molecular network in bladder cancer. Molecular Cancer Therapeutics. - Kung, L.H.W. et al. (2017) Utility of circulating serum miRNAs as biomarkers of early cartilage degeneration in animal models of post-traumatic osteoarthritis and inflammatory arthritis. Osteoarthritis and Cartilage.
- Stein, E. et al. (2017) Steps to achieve quantitative measurements of microRNA using two step droplet digital PCR. PLoS One.
LNA-enhanced miRNA sequencing
Resources:
QIAseq miRNA Library QC PCR Panel and Assays (formerly Exiseq NGS Sample QC Kit Small RNA/microRNA #800101–800105
- QIAseq miRNA Library QC PCR Handbook
- QIAseq miRNA Library QC Spike-Ins Quick-Start Protocol
- Explore the RNA Universe! Poster
QIAseq miRNA Library Kit
- QIAseq miRNA Library Handbook
- QIAseq miRNA Library Kit Quick-Start Protocol Part 1 (Protocols, Part 1: 3’ Ligation, 5’ ligation, Reverse-Transcription)
- QIAseq miRNA Library Kit Quick-Start Protocol Part 2 (Protocols, Part 2: QMN Bead Preparation, cDNA Cleanup, Library Amplification)
- QIAseq miRNA Library Kit Quick-Start Protocol Part 3 (Protocols, Part 3: Library Cleanup, miRNA Library Pre-Sequencing QC, Determining Library
- Explore the RNA Universe! Poster
- QIAseq miRNA Library Kit Product Profile
- Frequently asked question
Publications:
- Franklin, O. et al. (2018) Plasma micro-RNA alterations appear late in pancreatic cancer. Annals of Surgery.
- Note: Uses multiple LNA technology kits, including QIAseq miRNA Library QC Spike-ins and Human Panel I, V5, miRCURY LNA miRNA miRNome PCR Panel
- Decmann, A. et al. (2018) MicroRNA expression profiling in adrenal myelolipoma. The Journal of Clinical Endocrinology & Metabolism.
- Zhou, Y. et al. (2018) Exosomes from endothelial progenitor cells improve the outcome of a murine model of sepsis. Molecular Therapy.
- Janaszak-Jasiecka, A. et al. (2018) eNOS expression and NO release during hypoxia is inhibited by miR-200b in human endothelial cells. Angiogenesis.
High-throughput 3' RNAseq from ultralow amounts of RNA
Resources:
QIAseq UPX 3’ Transcriptome Kits
- QIAseq UPX3' Transcriptome Handbook
- QIAseq UPX 3' Transcriptome Library Kit Quick-Start Protocol (Part 1): Cell lysis and reverse transcription
- QIAseq UPX 3' Transcriptome Library Kit Quick-Start Protocol (Part 2): Template amplification and fragmentation, end-repair and A-addition
- QIAseq UPX 3' Transcriptome Kit Product Profile
- Template: Setup custom library prep kit in BaseSpace Sequence Hub (NextSeq)
QIAseq UPX 3’ Targeted RNA Panels
- QIAseq UPX 3' Targeted RNA Library Handbook
- QIAseq UPX3' Targeted RNA Panel Quick-Start Protocol (Part 1): Cell lysis, reverse transcription
- QIAseq UPX 3' Targeted RNA Panel Quick-Start Protocol (Part 2): Single primer extension
- QIAseq UPX 3' Targeted RNA Panel Product Profile
- Template Set A: Setup custom library prep kit in BaseSpace Sequence Hub (NextSeq)
- Template Set B: Setup custom library prep kit in BaseSpace Sequence Hub (NextSeq)
- Template Set C: Setup custom library prep kit in BaseSpace Sequence Hub (NextSeq)
- Template Set D: Setup custom library prep kit in BaseSpace Sequence Hub (NextSeq)
Publications:
- Malinska, D. et al. (2018) Assessment of mitochondrial function following short- and long-term exposure of human bronchial epithelial cells to total particulate matter from a candidate modified-risk tobacco product and reference cigarettes. Food and Chemical Toxicology.
- Kaur, S. et al. (2018) A function-blocking CD47 antibody modulates extracellular vesicle-mediated intercellular signaling between breast carcinoma cells and endothelial cells. Journal of Cell Communication and Signaling.
- Dobbs, K. et al. (2017) Monocyte dysregulation and systemic inflammation during pediatric falciparum malaria. JCI Insight.
Antisense LNA GapmeRs
Products:
- Antisense LNA GapmeRs
- Antisense LNA GapmeR Custom Plate
Resources:
- Antisense LNA GapmeR Design Tool Guide
- Antisense LNA GapmeR Design Tool Guide
- Antisense LNA GapmeRs Handbook
Publications:
- Forrest, M. et al. (2018) Colon cancer-upregulated long non-coding RNA lincDUSP regulates cell cycle genes and potentiates resistance to apoptosis. Scientific Reports.
- Polovic, M. et al. (2018) Identification of a novel lncRNA induced by the nephrotoxin ochratoxin A and expressed in human renal tumor tissue. Cellular and Molecular Life Sciences.
- Zhang, X. et al. (2018) Long non-coding RNA malat1 regulates angiogenesis in hindlimb ischemia. International Journal of Molecular Sciences.
- Mondal, T. et al. (2018) Sense-antisense lncRNA pair encoded by locus 6p22.3 determines neuroblastoma susceptibility via the USP36-CHD7-SOX9 regulatory axis. Cancer Cell.
- De Clara, E. et al. (2017) Long noncoding RNA expression profile in cytogenetically normal acute myeloid leukemia identifies a distinct signature and a new biomarker in NPM1-mutated patients. Haematologica.
miRCURY LNA miRNA Inhibitors, Mimics and Target Site Blockers
Resources:
- miRCURY LNA miRNA Inhibitors and Power Inhibitors
- Custom miRCURY LNA Inhibitors and Power Inhibitors
- miRCURY LNA miRNA Family Power Inhibitors
- miRCURY LNA miRNA Target Site Blockers
- miRCURY LNA miRNA Inhibitors and Target Site Blockers Handbook
miRCURY LNA miRNA Inhibitor Libraries
miRCURY LNA miRNA Mimics
Publications:
- Besharat, Z.M. et al. (2018) Foxm1 controls a pro-stemness microRNA network in neural stem cells. Scientific Reports. (Inhibitors)
- Wang, Z. et al. (2018) Parasitic insect-derived miRNAs modulate host development. Nature Communications. (Mimics)
- Zhang, X. et al. (2018) MYC is downregulated by a mitochondrial checkpoint mechanism. Oncotarget. (Inhibitors)
- Jones Ofori et al. (2017) Elevated miR-130a/miR130b/miR-152 expression reduces intracellular ATP levels in the pancreatic beta cell. Scientific Reports. (Inhibitors)
miRCURY LNA miRNA Detection Probes for ISH and Northern blotting
Resources:
miRCURY LNA miRNA Detection Probes Custom miRCURY LNA miRNA Detection Probes
miRCURY LNA miRNA ISH Optimization Kits (FFPE)
- miRCURY LNA miRNA ISH Optimization Kits (FFPE) Handbook
- miRCURY LNA miRNA ISH Optimization Kits (FFPE) Product Sheet
Publications:
- Fukuoka, M. et al. (2018) Supplemental treatment for Huntington’s disease with miR-132 that is deficient in Huntington’s disease brain. Molecular Therapy Nucleic Acids.
- Peng, S-Y. et al. (2018) miR‐134 targets PDCD7 to reduce E‐cadherin expression and enhance oral cancer progression. International Journal of Cancer.
- Ottaviani, S. et al. (2018) TGF-β induces miR-100 and miR-125b but blocks let-7a through LIN28B controlling PDAC progression. Nature Communications.
- Jiajiem T. et al. (2017) Conserved miR-10 family represses proliferation and induces apoptosis in ovarian granulosa cells. Scientific Reports.
Custom LNA mRNA Detection Probes for ISH
Resources:
Publications:
- Madelaine, R et al. (2018) A screen for deeply conserved non-coding GWAS SNPs uncovers a MIR-9-2 functional mutation associated to retinal vasculature defects in human. Nucleic Acids Research.
Custom LNA Oligonucleotides
Products:
Resources:
- Custom LNA Oligonucleotides Tools and resources
Publications:
- Santolla, M. et al. (2018) miR-221 stimulates breast cancer cells and cancer-associated fibroblasts (CAFs) through selective interference with the A20/c-Rel/CTGF signaling. Journal of Experimental & Clinical Cancer Research.
- Zlatev, I. et al. (2018) Reversal of siRNA-mediated gene silencing in vivo. Nature Biotechnology.
- Colineau, L. et al. (2018) c-Myc is a novel leishmania virulence factor by proxy that targets the host miRNA system and is essential for survival in human macrophages. Journal of Biological Chemistry.
- Spasic, A. et al. (2018) Molecular dynamics correctly models the unusual major conformation of the GAGU RNA internal loop and with NMR reveals an unusual minor conformation. RNA.
- De Simone, V. et al. (2017) Smad7 knockdown activates protein kinase RNA-associated eIF2α pathway leading to colon cancer cell death. Cell Death & Disease.
SNP detection using LNA Oligonucleotides
Publications:
Tian, K. et al. 2018) Single locked nucleic acid-enhanced nanopore genetic discrimination of pathogenic serotypes and cancer driver mutations. ACS Nano.
Publications using multiple LNA kits or products
- Schneider, R. et al. (2018) Downregulation of exosomal miR-204-5p and miR-632 as a biomarker for FTD: a GENFI study. Journal of Neurology, Neurosurgery and Psychiatry.
- Quan, Y.S. et al. (2018) Knockdown of long non-coding RNA MAP3K20 antisense RNA 1 inhibits gastric cancer growth through epigenetically regulating miR-375. Biochemical and Biophysical Research Communications.
- Chen, K. et al. (2018) Methylation-associated silencing of miR-193a-3p promotes ovarian cancer aggressiveness by targeting GRB7 and MAPK/ERK pathways. Theranostics.
- Nakka, M. et al. (2017) Biomarker significance of plasma and tumor miR-21, miR-221, and miR-106a in osteosarcoma. Oncotarget.