PCR/qPCR/dPCR
Spezifische, sensitive, robuste und zuverlässige PCR
Bei der Durchführung von Genamplifikationen kann der Erfolg Ihrer Experimente von mehreren kritischen Faktoren abhängen, einschließlich Durchsatz, Assay-Sensitivität, Robustheit der Reaktion auch bei schwierigen Templates und zuverlässiger Datennormalisierung. Sie müssen möglicherweise auch die besonderen Herausforderungen in Verbindung mit der verwendeten PCR-Methode überwinden.
Egal welche Methode Sie verwenden, wir bieten eine Fülle optimierter Lösungen für Endpunkt-PCR, qPCR und dPCR an. Unsere Kits, Assays, Geräte und unser Zubehör helfen Ihnen, Ihre Targets zuverlässig zu amplifizieren und nachzuweisen, und ermöglichen die Optimierung Ihres Workflows für praktisch jede Applikation.
- Measure gene expression (mRNA, miRNA)
- Quantify DNA or RNA targets
- Detect & quantify pathogen and/or viral RNA/DNA
- Quantify residual DNA
- Convert RNA to cDNA for quantification
- Mutation detection (including rare/ultra-rare variants)
- Analyze copy number variation (CNV)
- Detect viral load
- Gene expression quantification
- Quantify low-abundance targets
- Measure residual DNA
- Amplify DNA for cloning or sequencing
- Qualitative pathogen detection (presence/absence)
- CRISPR screening
- Genotyping
- Convert RNA to cDNA for storage
- Cq/Ct values
- Relative quantification (ΔΔCq) or absolute quantification from standards
- cDNA for downstream use
- Absolute target quantification (copies/µL reaction)
- Fractional abundance (%)
- Mutation/target frequency
- Copy number per genome
- Amplified DNA or cDNA product
- Band visualization on gel
- Presence/absence result
- Isolated DNA or cDNA
- Isolated RNA/mRNA/miRNA
- Some kits support crude samples such as cells, blood, dried blood spots, saliva, buccal swabs, or gargle
- Isolated DNA or cDNA
- Isolated RNA/mRNA/miRNA
- Purified samples recommended
- Isolated DNA or cDNA
- Isolated RNA
- Quantitative real-time PCR
- Reverse transcription
- Amplification and fluorescence-based detection in real-time for quantification
- Quantitative digital PCR
- Partitions the sample into thousands of reactions and uses end-point PCR + Poisson statistics for absolute quantification without a standard curve
- Standard end-point PCR
- DNA amplification and visualization (e.g., gel-based) and downstream applications
- When reliable quantification is required over a wide linear range
- When standard curve-based or relative quantification is sufficient
- When precise quantification is required
- When detecting rare alleles or small fold changes
- When standard curves are undesirable
- When working near the detection limit (low-abundance targets)
- When qualitative detection without quantification is sufficient
- When preparing amplicons for cloning, genotyping, sequencing, or other downstream applications
- Real-time PCR thermal cycler
- Dye-based (e.g., SYBR Green) or probe-based detection
- QIAcuity dPCR platform
- Probe‑based or intercalating dye detection
- Thermal cycler
- Visualization (e.g., via agarose gel electrophoresis)
Durchsuchen PCR/qPCR/dPCR
Produkte
Sort
Reaction
Detection
Template
Starting material
Application
Cells transfected
PCR product type
Technology
Instrument
Regulatory Status
7 Produkte gefunden
QIAcuity High Multiplex Probe PCR Kit
For challenging samples and dPCR multiplexing beyond five targets
ID SOLUTIONS IDNAPTEX dPCR Assays
For detecting genomic alterations, such as point mutations, deletions and fusions in DNA extracted from liquid and tissue biopsies using multiplex digital PCR for cancer research applications
QIAprep& Plasmodium Kit
For direct qPCR detection of Plasmodium spp. from dried blood spots or whole blood.
QIAcuity RCL Quant Kit
For the detection of Replication Competent Lentivirus (RCL) with high accuracy using the QIAcuity digital PCR instruments
QIAprep& Plasmodium Assays
For distinguishing between Plasmodium falciparum and non-falciparum species, as well as differentiating four additional species
dPCR PanCancer Kits
For detection of multiple hallmark mutations in cancer-relevant genes using the QIAcuity digital PCR instruments
Q-Solution Kit
Für bessere Ergebnisse bei PCR-Reaktionen mit schwierigeren Setups enthält dieses Kit Q-Solution und MgCl2.