
dMIQE guidelines: Deciphering digital PCR analysis and statistics

Digital PCR allows the absolute quantification of targets without the use of any standards. In this webinar, the underlying statistics of the analysis will be discussed. In addition, key parameters such as the concentration factor lambda, partition volume, confidence intervals and dead volume will be explained. Special emphasis will be laid on adherence to the digital MIQE (dMIQE) and the updated dMIQE2020 guidelines.