PCR/qPCR/dPCR
满怀信心地进行特异性、灵敏且稳健的 PCR
在进行基因扩增时，您的实验成功与否取决于几个关键因素，其中包括通量、检测灵敏度、反应的稳健性（即使使用困难模板）以及可靠的数据均一化。您可能还需要克服与您正在使用的 PCR 方法相关的特定挑战。
无论您选择哪种方法，我们都能为终点法 PCR、qPCR 和 dPCR 提供丰富的优化解决方案。我们的试剂盒、assay、仪器和附件可帮助您在几乎任何应用种都能更有信心地扩增和检测靶标以及简化您的工作流程。
- Measure gene expression (mRNA, miRNA)
- Quantify DNA or RNA targets
- Detect & quantify pathogen and/or viral RNA/DNA
- Quantify residual DNA
- Convert RNA to cDNA for quantification
- Mutation detection (including rare/ultra-rare variants)
- Analyze copy number variation (CNV)
- Detect viral load
- Gene expression quantification
- Quantify low-abundance targets
- Measure residual DNA
- Amplify DNA for cloning or sequencing
- Qualitative pathogen detection (presence/absence)
- CRISPR screening
- Genotyping
- Convert RNA to cDNA for storage
- Cq/Ct values
- Relative quantification (ΔΔCq) or absolute quantification from standards
- cDNA for downstream use
- Absolute target quantification (copies/µL reaction)
- Fractional abundance (%)
- Mutation/target frequency
- Copy number per genome
- Amplified DNA or cDNA product
- Band visualization on gel
- Presence/absence result
- Isolated DNA or cDNA
- Isolated RNA/mRNA/miRNA
- Some kits support crude samples such as cells, blood, dried blood spots, saliva, buccal swabs, or gargle
- Isolated DNA or cDNA
- Isolated RNA/mRNA/miRNA
- Purified samples recommended
- Isolated DNA or cDNA
- Isolated RNA
- Quantitative real-time PCR
- Reverse transcription
- Amplification and fluorescence-based detection in real-time for quantification
- Quantitative digital PCR
- Partitions the sample into thousands of reactions and uses end-point PCR + Poisson statistics for absolute quantification without a standard curve
- Standard end-point PCR
- DNA amplification and visualization (e.g., gel-based) and downstream applications
- When reliable quantification is required over a wide linear range
- When standard curve-based or relative quantification is sufficient
- When precise quantification is required
- When detecting rare alleles or small fold changes
- When standard curves are undesirable
- When working near the detection limit (low-abundance targets)
- When qualitative detection without quantification is sufficient
- When preparing amplicons for cloning, genotyping, sequencing, or other downstream applications
- Real-time PCR thermal cycler
- Dye-based (e.g., SYBR Green) or probe-based detection
- QIAcuity dPCR platform
- Probe‑based or intercalating dye detection
- Thermal cycler
- Visualization (e.g., via agarose gel electrophoresis)
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DNA methylation analysis
找到 156 个产品
QIAcuity High Multiplex Probe PCR Kit
For challenging samples and dPCR multiplexing beyond five targets
ID SOLUTIONS IDNAPTEX dPCR Assays
For detecting genomic alterations, such as point mutations, deletions and fusions in DNA extracted from liquid and tissue biopsies using multiplex digital PCR for cancer research applications
QIAprep& Plasmodium Kit
For direct qPCR detection of Plasmodium spp. from dried blood spots or whole blood.
QIAprep& Plasmodium Assays
For distinguishing between Plasmodium falciparum and non-falciparum species, as well as differentiating four additional species
QIAcuity RCL Quant Kit
For the detection of Replication Competent Lentivirus (RCL) with high accuracy using the QIAcuity digital PCR instruments
QIAGEN OneStep RT-PCR Kit
用于灵敏、特异性的一步法RT-PCR
QIAGEN Multiplex PCR Kit
用于高特异性和灵敏度的多重PCR，无需优化
QuantiTect Reverse Transcription Kit
快速合成cDNA，进行两步法real-time RT-PCR，用于基因表达分析
HotStarTaq DNA Polymerase
高特异性扩增，所需优化次数少
RT2 Profiler PCR Arrays
用于可靠、灵敏的基因表达谱分析
REPLI-g Single Cell Kit
对单个细胞或有限的样本进行高度均一的全基因组扩增（WGA）
QuantiTect RT-PCR Kit
用于使用 SYBR® Green I 进行一步法 qRT-PCR 以及使用序列特异性探针进行基因表达分析的一步法 qRT-PCR 和多重 qRT-PCR
RT2 SYBR® Green qPCR Mastermixes
用于real-time PCR分析
HotStarTaq Master Mix Kit
高特异性扩增，用于多种PCR分析
QuantiTect PCR Kit
适用于使用 SYBR Green I 的 qPCR 和两步法 qRT-PCR，qPCR 和两步法 qRT-PCR，以及使用序列特异性探针的多重 qPCR 和两步法多重 qRT-PCR
Rotor-Gene Q
表现卓越的定量PCR
QuantiFast Pathogen +IC Kit
用于灵敏而又可靠地检测病毒 RNA/DNA 和细菌 DNA，包括内质控
HotStarTaq Plus DNA Polymerase
快速、高特异性扩增，适合多种应用
miRCURY LNA SYBR® Green PCR Kits
For high-performance PCR with the miRCURY LNA miRNA PCR System
Taq DNA Polymerase
用于常规和特殊的PCR
QuantiTect Primer Assays
使用SYBR® Green进行两步法和一步法定量RT-PCR，用于基因表达分析
miRCURY LNA miRNA PCR Assay
使用基于 LNA 优化、SYBR ® Green 的 miRNA PCR系统，实现极其灵敏和特异的 miRNA 定量
miRCURY PCR Controls
Validated primer sets for normalization of miRNA expression levels in real-time PCR analyses
RT2 First Strand Kit
用于更快、更高效地合成cDNA，并去除RNA样本中的基因组DNA
miRCURY LNA RT Kit
使用 SYBR Green 或水解探针与 miRCURY LNA miRNA PCR 系统进行最佳的第一链 cDNA 合成
HotStarTaq Plus Master Mix Kit
快速、高特异性扩增，适合多种应用
Custom Microbial DNA qPCR Arrays
real-time PCR方法的微生物鉴定或分析
QuantiTect Virus Kit
适用于高灵敏度检测病毒 RNA 或 DNA
RT2 qPCR Primer Assays
准确、可靠的基因表达分析
Type-it Microsatellite PCR Kit
快速可靠的微卫星位点多重PCR分析
QuantiNova PCR Kit
适用于使用基于 SYBR Green 的 qPCR 进行高灵敏度、特异性和超快探针法 Real-time PCR 和多重 PCR 以及获得无与伦比的结果
QIAGEN Multiplex PCR Plus Kit
用于成功的进行多重PCR反应
Omniscript RT Kit
在终点法PCR中，用于RNA模板量为50 ng到2 μg的逆转录反应
Custom RT2 Profiler PCR Arrays
用于可靠、灵敏的基因表达谱分析
Microbial DNA qPCR Assays
采用real-time PCR技术进行微生物检测
mericon Sample Preparation and Bacteria Kits
For extraction of high-quality DNA from microbial enrichment cultures and raw or processed foods