What is Plasmid DNA

DNA extraction technologies

Working with DNA Good laboratory practice

• Handling DNA
• Conversions for nucleic acids: DNA

Working with DNA Good microbiological practice

• Growth of E. coli strains
• The E. coli growth curve
• Storage of E. coli strains

Isopropanol precipitation of DNA

Storage of DNA

DNA cleanup

Plasmid specifications

Bacterial cultivation media and antibiotics

• Liquid media
• Sterilizing media
• Solid media
• Antibiotics

Lysis of bacterial cells for plasmid purification

• Alkaline lysis
• Other lysis methods
• Lysis of bacteria other than E. coli

Transformation of DNA

• Preparation of competent E. coli
• Transformation of competent E. coli
• Positive control to check transformation efficiency
• Negative control to check antibiotic activity

Endotoxins and what to consider

• What are endotoxins?
• Endotoxin contamination of different plasmid preparation methods
• How are endotoxins measured?
• Influence of endotoxins on biological applications
• Endotoxin-free plasticware and glassware

Quantification of DNA

Spectrophotometric measurement of DNA concentration

• Fluorometry
• Agarose gel

DNA analysis using analytical gels

• Pouring an agarose gel
• Running an agarose gel
• Visual analysis of the gel