Investigator IDplex Plus Kit

For multiplex amplification of 13 CODIS core loci, plus D2S1338, D19S433 and Amelogenin, also directly from swabs and papers

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Investigator IDplex Plus Kit (100)

Cat. No. / ID: 381625

Primer Mix, Fast Reaction Mix including HotStarTaq Plus DNA Polymerase, Control DNA, Allelic Ladder IDplex Plus, DNA Size Standard 550 (BTO), and Nuclease-Free Water

KitAccessories

Investigator IDplex Plus

Matrix Standard

Reactions

100

400

This product contains substances regulated under REACH (EC 1907/2006 Annex XIV). The use of this product in the EU is permitted subject to an exemption (Article 56(3)). Please refer to the REACH notification and the SDS of this product, both of which can be found in the “Resources” section of this page, for more information.

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Investigator IDplex Kits are intended for molecular biology applications in forensic, human identity and paternity testing. These products are not intended for diagnosis, prevention, or treatment of a disease. These products are not available in all countries.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

Simultaneous amplification of 16 loci incl. CODIS core
Highly suitable for forensic casework or reference samples
Straightforward protocols with ready-to-use Fast Reaction Mix
Optimized primer design to reduce marker overlap

Product Details

Investigator IDplex Plus Kit is a multiplex STR assay for human identification covering the 13 CODIS (Combined DNA Index System) core loci, D2S1338, D19S433 and Amelogenin.

The kits utilize our fast-cycling technology, enabling amplification in ca. 90 minutes. Highly sensitive with rapid and reliable results from trace DNA, the Investigator IDplex Plus Kit is suitable for all forensic applications and paternity testing. The genetic analyzer is calibrated with BT5 Matrix.

Investigator IDplex Kit meets ISO 18385 requirements.

Performance

Investigator IDplex Kit utilize fast-cycling technology to provide reliable results in STR assays in ca. 90 minutes from extracted DNA.

The Fast Reaction Mix is based on proven QIAGEN multiplex PCR technology that has been optimized to increase inhibitor tolerance. Assay performance tested in the presence of increasing concentrations of 6 types of inhibitor (humic acid, hematin, tannic acid, indigo carmine, calcium and collagen) shows high sensitivity and superior inhibitor resistance. This enables reliable, high-quality results from casework samples.

The Investigator IDplex Kit is validated and evaluated using the GeneAmp 9700 thermal cycler and the Applied Biosystems 3500 Genetic Analyzer.

The recommended amount of DNA under standard conditions for use with the Investigator IDplex Plus Kit is 0.5 ng. Internal validations demonstrated robust and balanced results with 0.2–2 ng DNA, and reliable results with <0.1 ng DNA.

Principle

Investigator IDplex Kit uses QIAGEN's fast-cycling PCR technology for the rapid, simultaneous amplification of the 13 CODIS (Combined DNA Index System) core loci, D2S1338, D19S433 and Amelogenin. Carefully designed primers reduce overlap of loci alleles, thereby minimizing the likelihood of misinterpretation.

Procedure

The 15 polymorphic autosomal STR markers (D2S1338, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D19S433, D21S11, CSF1PO, FGA, TH01, TPOX and vWA), and the gender-specific Amelogenin are amplified simultaneously in a single PCR and labeled with one of the dyes listed in the table "Investigator IDplex Plus Kit dyes and corresponding markers".

Investigator IDplex Plus Kit dyes and corresponding markers
Dye	Marker
6-FAM	Amelogenin	TH01	D3S1358	vWA	D21S11
BTG	TPOX	D7S820	D19S433	D5S818	D2S1338
BTY	D16S539	CSF1PO	D13S317	FGA
BTR	D18S51	D8S1179

The amplified loci then undergo capillary electrophoresis. The results can be analyzed using dedicated software, such as GeneMapper ID-X. See the table "Technical specifications" for more information.

Applications

Investigator IDplex Plus Kits are for rapid and reliable generation of DNA profiles from blood, buccal swabs and forensic stains or reference samples and are highly suited for:

Criminal database, forensic reference or casework samples
Paternity testing
Population genetics studies
Other human identity testing applications

Technical specifications
Features	Investigator IDplex Plus Kit
DNA input volume	Up to 15 µl
Volume per reaction	25 µl
Matrix	BT5
Fluorescence dyes	6-FAM, BTG, BTY, BTO
Genetic analyzers	ABI PRISM 3100/3100 Avant, Applied Biosystems 3130/3130xl, or Applied Biosystems 3500 Genetic Analyzers
Software for analysis	Applied Biosystems GeneMapper ID or GeneMapper ID-X

Resources

Advanced sample collection, automation and STR solutions for kinship testing

Enjoy success at first try with high performance QIAGEN Multiplex STR amplification and instrumentation

The most comprehensive and versatile coverage of current international STR standards

Quality initiatives for human identity testing and forensics

For further information on which version of the v11 template files is suitable for your needs, please refer to the Template File Info Note.

For further information on which version of the v10 template files is suitable for your needs, please refer to the Template File Info Note.

For further information on which version of the v10 template files is suitable for your needs, please refer to the Template File Info Note.

Download Safety Data Sheets for QIAGEN product components.

FAQ

Yes. The Fast Reaction Mix of the Investigator IDplex Plus Kit contains HotStarTaq Plus enzyme, Mg2+, and dNTPs in a pre-optimized concentration. Only Investigator IDplex Plus Primer Mix and template DNA need to be added. Nuclease-free Water should be added to complete the reaction volume.

FAQ ID -3008

We recommend first adding master mix to the PCR plate and then adding the punch to avoid punches getting lost or mixed up due to electrostatic effects. Furthermore, we strongly recommend spinning down the PCR plate after setup to ensure all punches get fully submerged.

FAQ ID -3096

Yes. The protocol is generic and has successfully been tested on various commonly used instruments.

FAQ ID -3089

Yes. The protocol of the Investigator IDplex Plus Kit is generic and has been successfully tested on various commonly used instruments.

FAQ ID -3009

15 µl input volume can be used with the Investigator IDplex Plus Kit.

FAQ ID -3014

Amplification of Control DNA 9948 is appropriate as a PCR performance check. However, if you have access to reference samples at constant quality (for example, swabs or FTA donated by lab personnel), these samples may be used as additional in process controls.

FAQ ID -3094

If you are using an ABI PRISM 3100-Avant/3100 or 3130/3130xl Genetic Analyzer, please use Any5Dye, if available. If you are using the Applied Biosystems 3500/3500xL Genetic Analyzer, please use filter set AnyDye. Otherwise, filter set G5 should be used. If you are using an ABI PRISM 310 Genetic Analyzer, filter set G5 should be used.

FAQ ID -3017

No. Unlike some other QIAGEN STR products (e.g., the Investigator Argus Y-12 QS Kit) there is no QS quality sensor included in the Investigator IDplex Plus Kit.

FAQ ID -3011

No. Up to 2 µl can be added on top of a 25 µl reaction.

FAQ ID -3088

We do not recommend using more than one punch of 1.2 mm diameter for blood or buccal cells on FTA, or 2 µl of a crude buccal swab lysate. To adapt protocols to the type and quality of samples used in your laboratory, and to the CE instrumentation in use, adjust PCR cycle numbers and CE injection conditions.

FAQ ID -3090

Yes. Use of indicator cards helps to ensure sample is taken from an appropriate area of the FTA card. This may significantly improve success rates compared to procedures using non-indicator cards.

FAQ ID -3095

The optimum sample DNA input range of the Investigator IDplex Plus Kit is between 200–500 pg. However, reliable results can be obtained with less than 100 pg. For higher amounts of input DNA, consider adjusting the PCR cycle number.

FAQ ID -3015

No. Amplification products of marker FGA have a slightly different size between IDplex Plus and IDplex GO! kits due to a change in the fluorescent dye linker used. Use of the IDplex Plus ladder will frequently cause off ladder calls for FGA alleles.

FAQ ID -3093

Yes. The Fast Reaction Mix contains Taq enzyme, Mg²⁺, and dNTPs in a pre-optimized concentration. Only IDplex GO! Primer Mix and sample needs to be added.

FAQ ID -3087

Yes. Sample materials used for direct amplification vary with regard to quality and quantity of DNA present, depending on sampling materials, procedures, and storage conditions. In order to obtain the highest possible success rates in routine use, it is essential to run a representative batch of samples for evaluation of optimal conditions. Furthermore, we recommend revisiting this aspect after a certain period of routine use to ensure the evaluated conditions are optimal and re-adjust if necessary.

FAQ ID -3091

No, the primer site locations of the markers in the Investigator IDplex Plus Kit have not been changed. Only minor changes have been introduced for some markers, e.g., to improve A addition to the PCR products.

FAQ ID -3012

Matrix BT5 should be used with Investigator IDplex Plus Kit.

FAQ ID -3016

The BT5 matrix standard is designed for use with our Investigator STR and Dipplex Assays. We do not disclose the containing dyes.

FAQ ID - 3364

Yes. For example, cycle numbers can be increased to 32 or 34 in order to obtain higher signals for very diluted samples. However, please note that this promotes the amplification of nonspecific products or amplification from spurious background DNA.

FAQ ID -3010

The Investigator IDplex Plus Kit contains Primer mix, Fast Reaction Mix, Control DNA allelic ladder IDplex Plus, DNA size standard 550 (BTO), and nuclease-free water.

FAQ ID -3013

The template files for the Investigator IDplex Plus Kit for various softwares and analyzers can be downloaded from the Resource tab on the product webpage.

FAQ ID -3019

No, primer site locations of markers have not been changed. Two additional SNP primers have been added to allow balanced amplification of vWA and D16S539 alleles carrying known primer binding site mutations. The mutations covered were observed in concordance studies in samples of African origin at an elevated level. The labelled FGA forward primer has been slightly changed with regard to the linker of the fluorescent dye. The change does not affect PCR amplification, but causes a slight size shift during capillary electrophoresis. The allelic ladder therefore has been changed accordingly.

FAQ ID -3092

For Any5Dye filter set with the Investigator IDplex Plus Kit, please use:

Condition Bound: Lower 1.0 – Upper 20
Sensitivity: 0.1
Quality Value: 0.80

FAQ ID -3018