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How precise is the QIAgility?
FAQ ID -3063
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How long does it take to set up a 96-well plate PCR reaction on the QIAgility?
FAQ ID -3066
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Can I use a bar code scanner or reader on the QIAgility?
FAQ ID -3067
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Can I use a USB drive to transfer protocols to BioSprint?
FAQ ID -3072
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Can BioSprint 96 be connected to LIMS?
FAQ ID -3070
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How can I find the run parameters for a BioSprint protocol that I am running?
FAQ ID -3069
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Can the FastLane Cell lysates (containing stabilized RNA, step 8. in Fastlane protocol) be stored?
FAQ ID -3077
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Can I use specific primers with the FastLane Kits?
FAQ ID -3078
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What is the expected level of endotoxins in plasmid DNA purified with QIAGEN Plasmid kits, QIAfilter Plasmid kits and HiSpeed Plasmid purification kits?
FAQ ID -3082
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What is the expected level of endotoxins in plasmid DNA purified with QIAprep spin miniprep kit?
FAQ ID -3081
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What is the concentration of the different Proteinase K solutions sold by QIAGEN?
FAQ ID -3084
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Is the Fast Reaction Mix ready to use in the Investigator IDplex GO?
FAQ ID -3087
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Where can I access Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit?
FAQ ID -3085
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Is it possible to use a standard processor — the kind used routinely for formalin-fixed samples — for dehydration and paraffin infiltration of PAXgene Tissue treated samples?
FAQ ID -3032
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Are PAXgene Tissue Containers suitable for long term-storage at freezing temperatures?
FAQ ID -3030
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Is it possible to manually process PAXgene Tissue treated samples without using an automated processor?
FAQ ID -3036
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Is a special processing protocol needed?
FAQ ID -3035
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Is it possible to process formalin-fixed and PAXgene Tissue fixed samples together in one run?
FAQ ID -3033
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Is it necessary to clean a processor normally used for formalin-fixed tissue before using it with PAXgene Tissue fixed tissue?
FAQ ID -3034
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Can sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for other histochemical staining techniques, such as PAS?
FAQ ID -3041
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What is the maximum DNA input volume that can be used in the Investigator IDplex Plus Kit?
FAQ ID -3014
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What is the optimal sample DNA concentration for the Investigator IDplex Plus Kit?
FAQ ID -3015
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What is the optimal sample DNA concentration for the Investigator ESSplex SE Plus Kit?
FAQ ID -3028
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What is the maximum DNA input volume that can be used in the Investigator ESSplex SE Plus Kit?
FAQ ID -3027
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What is the correct matrix to use with Investigator ESSplex SE Plus Kit?
FAQ ID -3029
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What is the correct matrix to use with Investigator IDplex Plus Kit?
FAQ ID -3016
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Which settings for the filter set Any5Dye must be adjusted before running the spectral calibration for Investigator ESSplex SE Plus Kit?
FAQ ID -3031
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When should the N2 bottle used with the QIAxcel Advanced System be changed?
FAQ ID -3001
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Which filter set should be used with Investigator IDplex Plus Kit?
FAQ ID -3017
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Which settings for the filter set Any5Dye must be adjusted before running the spectral calibration for Investigator IDplex Plus Kit?
FAQ ID -3018
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Which filter set should be used with Investigator ESSplex SE Plus Kit?
FAQ ID -3030
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Where can I find the template files for the Investigator ESSplex SE Plus Kit?
FAQ ID -3032
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What does the Investigator IDplex Plus Kit contain?
FAQ ID -3013
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Where can I find the template files for the Investigator IDplex Plus Kit?
FAQ ID -3019
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Does the Investigator IDplex Plus assay contain an internal control?
FAQ ID -3011
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Does the Investigator ESSplex SE Plus assay contain an internal control?
FAQ ID -3023
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Have primer sequences of the Investigator ESSplex SE Plus Kit been changed compared to the former Investigator ESSplex SE assay?
FAQ ID -3025
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Can the PCR protocol of the Investigator IDplex Plus Kit be run on different PCR endpoint cyclers?
FAQ ID -3009
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Can the PCR protocol of the Investigator ESSplex SE Plus Kit be run on different PCR endpoint cyclers?
FAQ ID -3021
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Are the multicopy reference assays for Type-it CNV PCR +qC Kits and the qBiomarker assays the same?
FAQ ID -3000
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Have primer sequences of the Investigator IDplex Plus Kit been changed compared to the former Investigator IDplex assay?
FAQ ID -3012
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Is the Fast Reaction Mix of the Investigator ESSplex SE Plus Kit ready to use?
FAQ ID -3020
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Is the Fast Reaction Mix of the Investigator IDplex Plus Kit ready to use?
FAQ ID -3008
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How should I reconstitute the lyophilized RNase-Free DNase Set with the RNeasy 96 kit?
FAQ ID -3003
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What does the Investigator ESSplex SE Plus Kit contain?
FAQ ID -3026
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Is it possible to extend the number of PCR cycles to increase sensitivity of the Investigator IDplex Plus Kit?
FAQ ID -3010
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How do I clean up RNA preparations containing miRNA?
FAQ ID -3002
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Is it possible to extend the number of PCR cycles to increase sensitivity of the Investigator ESSplex SE Plus Kit?
FAQ ID -3022
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What is the thermal output for a BioRobot MDx and BioRobot Universal?
FAQ ID -3056
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What is the thermal output for a QIAsymphony SP and AS instrument?
FAQ ID -3057
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Can I use Tungsten Carbide beads for RNA extraction protocols using QIAGEN Kits?
FAQ ID -3053
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What is the minimum number of samples that can be extracted on a BioSprint 96?
FAQ ID -3076
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What is the fixation method used in the PAXgene Tissue System?
FAQ ID -3019
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What does PAXgene Tissue FIX contain?
FAQ ID -3020
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Can I perform the IDH 1/2 RGQ PCR test with slides containing less than 40% tumor cells?
FAQ ID -3098
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What volume of bacterial culture can be processed using the QuickLyse Miniprep Kit?
FAQ ID -1357
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Can the MaXtract High Density Tube be used with QIAzol to isolate RNA?
FAQ ID -1304
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I have a Human Druggable Genome siRNA Set V2.0. Can I reorder the siRNAs from this set?
FAQ ID -1366
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What factors determine if denatured proteins accumulate underneath, or inside the gel of the MaXtract Low and High Density Tubes?
FAQ ID -1312
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If both MaXtract Low Density and High Density is suitable for an application, which type is preferable to use?
FAQ ID -1310
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How will service work carried out on my QIAGEN BioRobot be documented?
FAQ ID -1331
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If I cannot order FlexiPlate siRNA online, how do I order?
FAQ ID -1371
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What information about my gene or siRNA do I need to order FlexiPlate siRNA?
FAQ ID -1365
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Is it possible to use MaXtract for an organic extraction with a mixture of phenol and BCP (1 bromine chlorpropane)?
-3
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What information is provided with FlexiPlate siRNAs?
FAQ ID -1363
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Is it possible to elute plasmid DNA obtained with the QuickLyse Miniprep Kit in nuclease-free water?
FAQ ID -1355
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Which formats are available for FlexiPlate siRNA?
FAQ ID -1358
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Which parts are exchanged during a Preventive Maintenance visit for my QIAGEN BioRobot?
FAQ ID -1334
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When isolating DNA from plant cells using CTAB, should MaXtract Low or High Density be used?
FAQ ID -1308
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Which controls can I choose for FlexiPlate siRNA?
FAQ ID -1368
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Will the REPLI-g UltraFast Mini Kit reaction always yield 10 µg DNA?
FAQ ID -1328
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Why is there DNA in the no-template control reaction when using the standard REPLI-g procedure, but not when using the UltraFast procedure?
FAQ ID -1327
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Which density gel type is contained in the yellow or green MaXtract Tubes?
FAQ ID -1315
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What is the composition of FlexiPlate siRNA buffer?
FAQ ID -1361
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Which software updates are included in a Premium Cover Agreement for my QIAGEN Instrument?
FAQ ID -1336
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What is the composition of elution buffer QLE in the QuickLyse Miniprep Kit?
FAQ ID -1356
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Can I reduce the amount of starting material below 10 ng when using the REPLI-g UltraFast Mini Kit?
FAQ ID -1325
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Can MaXtract tubes be autoclaved prior to use?
FAQ ID -1305
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Can I reduce the reaction time to e.g., 30 minutes, when using the REPLI-g UltraFast Mini Kit?
FAQ ID -1326
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Can I use any type of DNA extraction method to purify template DNA for amplification using the REPLI-g Mitochondrial DNA Kit?
FAQ ID -1321
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Can the bacterial pellet in the QuickLyse Miniprep procedure be resuspended by pipetting up and down or shortening the vortexing time?
FAQ ID -1352
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Can several extraction steps be performed in the same MaXtract tube?
FAQ ID -1302
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Can a phenol-chloroform ratio different from 1:1 also be used with MaXtract Low and High Density?
FAQ ID -1309
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Can DNA isolated with MaXtract tubes be used for downstream applications such as restriction digestion, labeling, blotting, PCR, and automated sequencing?
FAQ ID -1303
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Can I amplify mitochondrial DNA directly from whole blood or cells using the REPLI-g Mitochondrial DNA Kit?
FAQ ID -1319
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Can I order Alexa Fluor labeled controls with FlexiPlate siRNA?
FAQ ID -1369
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Can frozen sample material pulverized in liquid nitrogen be directly added to MaXtract tubes?
FAQ ID -1301
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Can HP Validated siRNAs be ordered on the FlexiPlate?
FAQ ID -1367
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Can I also screen large constructs like BACs, fosmids, and cosmids using the QIAGEN PlasmidAmp Kit?
FAQ ID -1337
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How do I resuspend my FlexiPlate siRNAs?
FAQ ID -1360
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How much REPLI-g amplified mitochondrial DNA should be used in a subsequent PCR reaction?
FAQ ID -1320
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Do mutations in the target mitochondrial DNA prevent primer binding, resulting in no DNA amplification with the REPLI-g Mitochondrial DNA Kit?
FAQ ID -1323
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Do you have stability data for genomic DNA isolated with the BioSprint Kits?
FAQ ID -1318
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Do customers have to comply with the in vitro diagnostics (IVD) guideline?
FAQ ID -1381
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Does the addition of sample and organic solution to the MaXtract Tubes require a specific pipetting sequence?
FAQ ID -1307
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Do you have stability data for genomic DNA isolated with the Flexigene DNA Kit?
FAQ ID -1317
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How does a Service Support Agreement provide defined budgeting for maintaining my BioRobot?
FAQ ID -1330
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How can I upload my gene list for FlexiPlate siRNA to GeneGlobe?
FAQ ID -1364
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How can I increase the DNA yield from low-copy plasmids using the QuickLyse Miniprep Kit?
FAQ ID -1354
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How can I ensure optimal performance of my QIAGEN instrument?
FAQ ID -1329
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What is the "seed sequence" of a miRNA?
FAQ ID -2262
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What is the throughput of the QIAsymphony when using the DSP AXpH protocol?
FAQ ID -2271
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What is the turn around time for the ordering of miScript Target Protector?
FAQ ID -2254
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What is the sample throughput of Pyrosequencing systems?
FAQ ID -2215
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What is the processing time for 96 normalizations with the SeqTarget System?
FAQ ID -2237
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What is the optimal range of PCR fragment sizes for normalization with the SeqTarget System?
FAQ ID -2247
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What method do you recommend to quantify DNA before running the KRAS and EGFR PCR kit?
FAQ ID -2292
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What technologies are used by the KRAS and EGFR PCR kits?
FAQ ID -2286
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What pathogens can be detected with the artus HSV/2 RG PCR Kits, and what is the LOD?
-1
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What kind of automation can be done with the EasyXtal 15-well Tool?
FAQ ID -2220
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What is the intended use of the QIAsymphony DSP AXpH kit?
FAQ ID -2273
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What kind of reading length can I expect when using Pyrosequencing technology for sequence analysis?
FAQ ID -2216
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What dyes are used in the KRAS and EGFR PCR kits?
FAQ ID -2288
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What do I do if I get amplification in my no template control (NTC) with the KRAS and EGFR PCR kits?
FAQ ID -2298
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What format are the EasyXtal 15-well tools?
FAQ ID -2219
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What downstream applications can I use the eluates from the QIAsymphony DSP AXpH DNA Kit?
FAQ ID -2275
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What do I need to provide when ordering a miScript Target Protector?
FAQ ID -2251
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What is the difference between the Influenza Master and the Influenza H1 Master of the artus Infl./H1 LC/RG RT-PCR Kit?
FAQ ID -2207
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What is the difference of the sequence of a miScript miRNA Inhibitor and miScript Target Protector?
FAQ ID -2253
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What is a miScript Precursor Assay?
FAQ ID -2214
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What is the input sample volume when using the QIAsymphony DSP AXpH kit?
FAQ ID -2272
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What is the difference between probability and typicality in the Rotor-Gene ScreenClust HRM Software?
FAQ ID -2203
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What is a miScript Target Protector?
FAQ ID -2249
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Which transfection reagent is recommended for transfection of the miScript Target Protectors?
FAQ ID -2252
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Which variants of the EasyXtal 15-well tool are available?
FAQ ID -2221
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Why are most of my samples outside of the cluster in supervised mode using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2205
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Which sample materials are validated within the CE mark for the artus HSV/2 RG PCR
-1
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Which sample materials can be tested using the artus Infl./H1 LC/RG RT-PCR Kit?
FAQ ID -2209
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Why are some of my samples outside of the cluster using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2204
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Why does the SeqTarget Prep Protocol require a 2-step procedure?
FAQ ID -2238
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Why does the miScript Target Protector not induce the degradation process?
FAQ ID -2263
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Why do I have wavy DNA bands on my agarose gel?
FAQ ID -2260
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What will happen, if the normalized DNA for the SeqTarget System is eluted with Buffer EB instead of EB2?
FAQ ID -2242
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Which sample material is validated within the CE mark for artus VZV RG PCR Kit?
FAQ ID -2231
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When negative results are generated using the KRAS and EGFR PCR kits, do I rerun them?
FAQ ID -2294
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What technologies are used by the therascreen PCR kits?
FAQ ID -2287
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What type of samples can I use for the KRAS PCR and EGFR kits?
FAQ ID -2293
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What voltage should be used to run my agarose gel with the GelPilot Agarose?
FAQ ID -2261
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Which pathogens can be detected using the artus Infl./H1 LC/RG RT-PCR Kit?
FAQ ID -2206
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Which mode should I use in the Rotor-Gene ScreenClust HRM Software, supervised or unsupervised?
FAQ ID -2202
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Which real-time PCR cyclers can be used with the artus Infl./H1 LC/RG RT-PCR Kits?
FAQ ID -2208
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Does the atypical look of the lyophilized FlexiTube siRNA Premix have a negative impact on performance?
FAQ ID -2267
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Does the miScript Target Protector match the target sequence?
FAQ ID -2250
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Do the KRAS and EGFR PCR kits contain a passive reference dye?
FAQ ID -2295
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Do I have to order at least 4 FlexiTube siRNA Premixes?
FAQ ID -2266
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How do I store my FlexiTube siRNA Premix?
FAQ ID -2270
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How is ARMS technology superior to alternative methods such as dye terminator sequencing?
FAQ ID -2290
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Effects of low A260/A230 ratios in RNA preparations on downstream applications
FAQ ID -2248
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How long can the extracted DNA from the QIAsymphony DSP AXpH DNA Kit be stored?
FAQ ID -2274
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How can I check the success of the normalization procedure for the SeqTarget System?
FAQ ID -2245
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How do I search for all the miScript Target Protectors for one species?
FAQ ID -2255
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Does the artus HSV/2 RG PCR kits cross-react with any other pathogens?
-1
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Can PCR fragments be normalized for the SeqTarget System after purification with QIAquick?
FAQ ID -2240
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Can PCR fragments be normalized for the SeqTarget System without a previous purification step?
FAQ ID -2246
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Can I use a 384 well plate for the KRAS and EGFR PCR kits?
FAQ ID -2296
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Are all 3 components of the SeqTarget System required or can a different Long Range PCR Kit and primers be used?
FAQ ID -2234
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Can the miScript Precursor Assay differentiate between pri- and pre-miRNA?
FAQ ID -2211
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Does ethanol from the PCR product purification influence the normalization procedure for the SeqTarget System?
FAQ ID -2244
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What are EasyXtal 15-well Tools used for?
FAQ ID -2217
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What are Principal Components analyzed in Rotor-Gene ScreenClust HRM Software?
FAQ ID -2200
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Is the SeqTarget System compatible with standard-size PCRs?
FAQ ID -2241
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What are the amplicon lengths of the KRAS and EGFR PCR kits?
FAQ ID -2289
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What are clusters analyzed in the Rotor-Gene ScreenClust HRM Software?
FAQ ID -2201
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What does QIAGEN use for a positive control in precursor detection experiments?
FAQ ID -2212
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What controls are used in the KRAS and EGFR PCR kits to ensure reliable results?
FAQ ID -2291
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What do I do if I cannot see amplification in the FAM channel in my KRAS and EGFR PCR kits?
FAQ ID -2297
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What are the corresponding QIAGEN names for former Biotage instruments?
FAQ ID -2285
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What buffer conditions give the best resolution for agarose gel electrophoresis?
FAQ ID -2256
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What can be the reason for low DNA yields after normalization with the SeqTarget System?
FAQ ID -2239
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How much buffer should be used for agarose gel electrophoresis?
FAQ ID -2257
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How much DNA should be loaded per well of an agarose gel?
FAQ ID -2259
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How should gels be cast with the GelPilot Agarose so that optimal resolution is achieved?
FAQ ID -2258
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How long does it take to generate results using the KRAS and EGFR PCR kits?
FAQ ID -2299
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How long should PCR fragments be for the SeqTarget System?
FAQ ID -2236
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How many miScript Precursor Assays do you offer?
FAQ ID -2213
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Is it possible to use lower siRNA concentrations with FlexiTube siRNA Premix?
FAQ ID -2264
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Is one able to use FlexiTube siRNA Premix for transfection of primary adherent cell types?
FAQ ID -2268
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Is one able to use FlexiTube siRNA Premix for transfection of primary suspension cell types?
FAQ ID -2269
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If I am working with a difficult-to-transfect cell type or if I obtain only weak silencing effects what can I do?
FAQ ID -2265
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Is it possible to normalize to more or less than 500 ng with the SeqTarget System if needed?
FAQ ID -2235
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Is it possible to purify and normalize long range PCRs produced with PCR kits from other vendors for the SeqTarget System?
FAQ ID -2243
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Is it possible to purify viral RNA using FastLane Cell RT-PCR Kits?
FAQ ID -1484
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Why is the RT step with the QuantiFast RT Kits much shorter compared to QuantiTect RT Kits?
FAQ ID -1451
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What applications are offered on the QIAcube?
FAQ ID -1403
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Why is the activation time for HotStarTaq Plus Polymerase in the QuantiFast SYBR Green Kits different from that for QuantiFast Probe Kits?
FAQ ID -1449
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Why is the storage time for QuantiFast PCR Kits shorter than that for QuantiTect PCR Kits?
FAQ ID -1446
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Why is the reaction volume for QuantiFast PCR Kits lower than that for QuantiTect PCR Kits?
FAQ ID -1447
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Why is the QuantiFast denaturation step different for PCR and RT-PCR runs in the two-step protocol for the ABI 7500 and other cyclers?
FAQ ID -1442
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What is the minimum number of siRNAs per order? If I order 40 siRNAs, can I get those on 2 plates?
FAQ ID -1468
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Can purification columns from other suppliers be processed on the QIAcube?
FAQ ID -1406
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Can QIAcube protocol runs be interrupted, and subsequently be continued to completion?
FAQ ID -1414
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Are run reports and/or log files available on the QIAcube?
FAQ ID -1412
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Can I use more than 4 µl FastLane lysate in subsequent real-time RT-PCR?
FAQ ID -1480
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Are FlexiPlate siRNAs available at a higher final concentration than 10 µM?
FAQ ID -1476
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Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with QuantiFast Probe PCR Kits?
FAQ ID -1452
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Can different amounts of siRNA be ordered on one FlexiPlate?
FAQ ID -1473
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Can 2 µl reaction volumes be used with QuantiFast PCR Kits?
FAQ ID -1440
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Are there any specific cleaning solutions recommended for the QIAcube?
FAQ ID -1416
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Do the QuantiTect Primer Assays work with QuantiFast SYBR Green Kits?
FAQ ID -1439
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Do special settings have to be used for QuantiFast PCR Kits on the Eppendorf Mastercycler ep realplex?
FAQ ID -1437
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Do I need to buy special kits for the QIAcube?
FAQ ID -1402
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Do I need to discard partially used QIAcube tip racks?
FAQ ID -1418
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What information is required to reorder specific siRNAs from a siRNA Set in FlexiPlate format?
FAQ ID -1463
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What are the turnaround times for FlexiPlate siRNA orders?
FAQ ID -1478
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What are the dimensions and weight of the QIAcube?
FAQ ID -1407
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How much time will be saved when switching from standard cycling to fast cycling with QuantiFast Kits?
FAQ ID -1438
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How many reactions can I perform with the new QuantiFast Kits for real-time PCR?
FAQ ID -1425
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If FlexiPlate siRNA was accidentally redissolved in siRNA Suspension Buffer instead of water, resulting in a 2x buffer, would it affect performance of the siRNAs?
FAQ ID -1477
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How do you achieve fast cycling, yet still deliver the same performance in PCR as that achieved with standard cycling?
FAQ ID -1430
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Is it necessary to place the lids of the elution tube and column into the slots of the rotor adapter during processing on the QIAcube?
FAQ ID -1415
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Why is a 2-step (and not a 3-step) cycling protocol recommended for QuantiFast SYBR Green PCR Kits?
FAQ ID -1450
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Why do QuantiFast SYBR Green PCR Kits require such a high primer concentration?
FAQ ID -1443
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What QuantiFast Kit should be used on the Eppendorf Mastercycler ep realplex?
FAQ ID -1436
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What is the detection limit of the QuantiFast Kits for real-time PCR?
FAQ ID -1432
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Which kind of 96 and 384-well plates are available for FlexiPlate siRNAs?
FAQ ID -1459
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Which cell lines have been tested with FastLane Cell RT-PCR Kits?
FAQ ID -1485
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Can I adjust the ROX concentration in the QuantiFast master mix?
FAQ ID -1427
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Can FlexiPlate siRNAs be ordered in plates other than the standard NUNC 96 well V bottom plates?
FAQ ID -1458
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Can I follow the reaction setup for probe-based assays described in Appendix D of the FastLane Cell RT-PCR Handbook using 384-well plates?
FAQ ID -1482
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Can fast cycling be carried out with QuantiFast Probe PCR Kits and FRET probes?
FAQ ID -1433
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Can FlexiPlate siRNA be ordered in solution on dry-ice?
FAQ ID -1474
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Can I program my own protocols for the QIAcube?
FAQ ID -1405
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How can I load new protocols onto the QIAcube?
FAQ ID -1421
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How can I get software updates for the QIAcube?
FAQ ID -1413
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How do QuantiFast PCR Kits compare to QuantiTect PCR Kits for quantitative real-time PCR?
FAQ ID -1441
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Do you offer trial-kit sizes for the new QuantiFast Kits?
FAQ ID -1429
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How can I decontaminate the QIAcube system?
FAQ ID -1419
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Can the QIAcube heater/shaker be used independently from protocol runs?
FAQ ID -1422
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Can the QIAcube rotor adapters be autoclaved?
FAQ ID -1409
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Can the QIAcube centrifuge be used independently from protocol runs?
FAQ ID -1423
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Can QuantiFast PCR Kits be used on real-time PCR instruments without fast cycling options?
FAQ ID -1428
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Can the QIAcube be connected to a laboratory information management system?
FAQ ID -1411
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Can the QIAcube rotor and/or buckets be removed for cleaning?
FAQ ID -1410
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Does the high primer concentration required by QuantiFast SYBR Green PCR Kits impair annealing specificity?
FAQ ID -1444
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Does the master mix in the QuantiFast Kits contain dUTP to allow UNG treatments?
FAQ ID -1431
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Can the QuantiFast Probe PCR +ROX Vial mastermix be stored long-term after adding ROX dye?
FAQ ID -1435
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What are the Tms of PCR products obtained using the miScript System?
FAQ ID -1599
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Do you have a protocol for cleanup of REPLI-g amplified DNA?
FAQ ID -1545
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Should carrier DNA or RNA be used for nucleic acid extraction of Varicella Zoster Virus (VZV) and Herpes Simplex Virus (HSV) samples for artus® assays?
FAQ ID -1530
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What do I have to do if precipitates are visible in the lysis buffer (well 1 of the reagent cartridge [RCB])?
FAQ ID -1568
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What can be done if the protein pellet does not solubilize completely using the AllPrep DNA/RNA/Protein Mini Kit?
FAQ ID -1580
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What controls should be used for normalizing miRNA real-time PCR data obtained with the miScript System?
FAQ ID -1596
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What is Q-Bond used in the QIAGEN Fast Cycling PCR and QuantiFast Kits?
FAQ ID -1554
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On which basis are the species of Mycobacteria grouped in complexes?
FAQ ID -1535
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Will QuantiTect Primer Assays work at an annealing temperature of 60ºC with QuantiFast SYBR Green PCR and RT-PCR Kits?
FAQ ID -1553
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Which homogenization method do you recommend for tissues stored in Allprotect Tissue Reagent?
FAQ ID -1572
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Which disinfectants and cleaning agents do you recommend for the BioRobot EZ1 DSP?
FAQ ID -1561
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Which BioRobot EZ1 instruments can be upgraded to become CE-IVDcompliant?
FAQ ID -1560
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Can samples other than whole blood be used with the EZ1 DSP DNA Blood Kit?
FAQ ID -1564
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Can I run RUO protocols on the new or upgraded BioRobot EZ1 DSP?
FAQ ID -1563
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Can I use sample preparation cartridges from other suppliers together with the BioRobot EZ1 DSP instrument?
FAQ ID -1567
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Can different incubation times be used for the 2 hour amplification step in the QuantiTect Whole Transcriptome protocol?
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Can I get IQ/OQ documents from QIAGEN so that I can perform my own IQ/OQ?
FAQ ID -1558
View
Does residual Allprotect Tissue Reagent left behind on tissue samples interfere with DNA, RNA, and protein purification?
FAQ ID -1573
View
Is cDNA generated with the QuantiTect Whole Transcriptome Kit suitable for use in microarray analysis?
FAQ ID -1587
View
Is it mandatory to perform IQ/OQ?
FAQ ID -1559
View
Do you have a protocol for purification of genomic DNA from cultured cells using the QIAamp DNA Micro Kit?
FAQ ID -1547
View
Do you have a protocol for concentration of RNA in 96-well plates?
FAQ ID -1546
View
Do you have a protocol for purification of total RNA from fatty tissues using QIAzol Lysis Reagent and MaXtract High Density?
FAQ ID -1550
View
How can I track samples during collection, sample preparation, and downstream assay?
FAQ ID -1570
View
Is the annual preventive maintenance mandatory for the BioRobot EZ1 DSP?
FAQ ID -1556
View
Why are the fluorescence intensities of the HSV detection markedly lower than those of the HSV-1 detection using the artus® HSV 1/2 LC PCR Kit?
-2
View
Which real-time PCR kits are recommended downstream of the QuantiTect Whole Transcriptome Kit?
FAQ ID -1592
View
Why is the magnesium (Mg2+) solution supplied in a separate tube in the artus® Parvo B19 PCR Kits?
FAQ ID -1533
View
What must I do if error code 29 is displayed in the LCD?
FAQ ID -1562
View
What primers are used in the reverse-transcription step of the QuantiTect Whole Transcriptome procedure?
FAQ ID -1584
View
What is the maximum amount of RNA that can be used for amplification with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1591
View
Can the AllPrep DNA/RNA/Protein Mini Kit be used with fibrous or lipid tissues?
FAQ ID -1579
View
What starting amount of RNA purified using an miRNeasy Kit should be used with the miScript System?
FAQ ID -1597
View
Can samples in Allprotect Tissue Reagent undergo repeated freeze-thaws without degradation of DNA, RNA, and protein?
FAQ ID -1575
View
Can the QuantiFast Probe PCR +ROX Vial Kit be used on the ABI PRISM 7900 and Applied Biosystems 7500 cyclers?
FAQ ID -1555
View
Can RNA purified from formalin-fixed, paraffin-embedded (FFPE) tissue be used with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1583
View
Does Buffer APP in the AllPrep DNA/RNA/Protein Mini Kit contain acetone?
FAQ ID -1576
View
Can tissue homogenized in Buffer RLT Plus instead of Buffer RLT be used with the AllPrep DNA/RNA/Protein Mini Kit?
FAQ ID -1577
View
Does cDNA amplified with the QuantiTect Whole Transcriptome Kit correspond to full-length RNA transcripts?
FAQ ID -1585
View
Can tissue incubated in Allprotect Tissue Reagent be used for morphological studies?
FAQ ID -1571
View
Can less than 10 ng RNA be used with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1590
View
Can Allprotect Tissue Reagent be used to stabilize RNA, DNA and proteins in cells, bacteria, yeast, and plants?
FAQ ID -1574
View
Can degraded RNA be used with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1586
View
How much cDNA is sufficient for real-time PCR detection of miRNA transcripts generated with the miScript System?
FAQ ID -1598
View
How many samples can be run in parallel with the Rotor-Gene Q real-time PCR instrument?
FAQ ID -1519
View
How long can blood samples be stored before sample preparation using the EZ1 DSP DNA Blood system?
FAQ ID -1565
View
How long can eluates be stored after sample preparation using the BioRobot EZ1 DSP instrument?
FAQ ID -1569
View
What is the advantage of EpiTect MethyLight PCR Kits over QuantiTect Probe PCR Kits for methylation studies?
FAQ ID -2010
View
What is the advantage of using the Type-it Microsatellite PCR Kit?
FAQ ID -2059
View
What is the advantage of using the Type-it Mutation Detect PCR Kit?
FAQ ID -2064
View
What is a good starting amount of template DNA for PCR with the EpiTect MSP Kit?
FAQ ID -2005
View
What is elution Buffer ATE of the QIAsymphony DNA Investigator Kit?
FAQ ID -2029
View
What is the maximum lifetime of the QIAGEN rotor for the Centrifuge 4 and 4K?
-16
View
What is the maximum DNA yield from forensic samples processed with the QIAsymphony SP?
FAQ ID -2028
View
What is the principle behind the DNA Polymerase in the EpiTect MSP Kit?
FAQ ID -2003
View
Is it possible to use different sample types in one run with the MagAttract 96 cador Pathogen Kit?
FAQ ID -2072
View
What is the purpose of the unmethylated and unconverted control DNA of the EpiTect PCR Control DNA Set?
FAQ ID -2007
View
Is magnetic particle carry-over in the eluates on the BioSprint 96 normal?
FAQ ID -2073
View
What happens when using EpiTect MethyLight Assays on only partially bisulfite converted DNA template?
FAQ ID -2009
View
Should sample lysate volumes be exactly 200 µl, 500 µl, or 1000 µl when loaded on the QIAsymphony SP?
FAQ ID -2031
View
What are the expected PCR results when using EpiTect Control DNA on untreated or bisulfite-converted DNA?
FAQ ID -2011
View
What are the features of the expression vector used for QIAgenes Insect/Mammalia?
FAQ ID -2050
View
Why do casework-sample protocols for the QIAsymphony DNA Investigator Kit start with different lysate volumes (200 µl, 500 µl, or 1000 µl)?
FAQ ID -2035
View
Why is CoralLoad included in the Type-it Mutation Detect, but not in the Type-it Microsatellite PCR Kit?
FAQ ID -2068
View
Why is there more than one QIAgene for my gene of interest?
FAQ ID -2044
View
Why do pQE-T7 vectors of the QIAgenes E. coli system have an additional UAG amber stop codon?
FAQ ID -2043
View
Why is the Type-it Mutation Detect PCR Kit recommended for preamplification of SNPs?
FAQ ID -2067
View
Will the optimized DNA sequence for QIAgenes be provided?
FAQ ID -2045
View
Why is maximum amplicon size for the Type-it Microsatellite PCR Kit limited to 500 bp?
FAQ ID -2060
View
Why is special Type-it Fast SNP Probe PCR chemistry required for TaqMan® SNP Genotyping?
FAQ ID -2057
View
What sample volumes can be processed with the QIAsymphony Virus/Bacteria Kits?
FAQ ID -2037
View
What sample types can be processed using the QIAsymphony Virus/Bacteria protocols?
FAQ ID -2036
View
Can data be analyzed on the Rotor-Gene Q while a run is in progress?
FAQ ID -2091
View
Are there two vectors for protein expression using QIAgenes in insect or mammalian cells?
FAQ ID -2048
View
Are there recommendations for sensitive detection of underrepresented fragments using the Type-it Mutation Detect PCR Kit?
FAQ ID -2066
View
Can other probe technologies besides TaqMan® be used with the Type-it Fast SNP Probe PCR Kit?
FAQ ID -2058
View
Is mitochondrial DNA isolated with the QIAsymphony DNA Investigator Kit on the QIAsymphony SP?
FAQ ID -2030
View
Is a passive internal reference dye, like ROX, required on the Rotor-Gene Q to obtain reproducible results?
FAQ ID -2079
View
Is it possible to edit the sample sheet in the Rotor-Gene Q software after a PCR run?
FAQ ID -2092
View
Is it possible to import a standard curve from a previous PCR run on the Rotor-Gene Q?
FAQ ID -2093
View
What are the ideal and the largest PCR amplicon sizes when using the EpiTect MSP Kit?
FAQ ID -2004
View
What kind of samples should be processed using the large-volume protocol with the QIAsymphony DNA Investigator Kit?
FAQ ID -2034
View
Which expression systems can be used with the QIAgenes Insect/Mammalia expression constructs?
FAQ ID -2049
View
Which QIAsymphony Virus/Bacteria Kit size should be used for each protocol?
FAQ ID -2038
View
What types of reaction vessels are required for use in the Rotor-Gene Q?
FAQ ID -2085
View
Which capillary sequencers can be used for detection of amplicons from the Type-it Microsatellite PCR Kits?
FAQ ID -2063
View
Can the EpiTect PCR Control DNAs be used for mouse and rat assays?
FAQ ID -2008
View
Can the EpiTect PCR Control DNAs also be used as standard in real-time methylation specific quantification?
FAQ ID -2006
View
Can the protocols for purification of small RNAs from cells in the appendices of the AllPrep DNA/RNA 96 Handbook also be used with tissue samples?
FAQ ID -2001
View
Can solid samples be processed with the QIAsymphony DNA Investigator Kit on the instrument?
FAQ ID -2027
View
Can QIAGEN rotors used with the centrifuges 4C and 4KC also be used with 4-16 and 4-16K models?
-15
View
Can the MagAttract 96 cador Pathogen Kit also be used on the KingFisher 96 or MagMAX Express 96 instruments?
FAQ ID -2074
View
Do all QIAsymphony DNA Investigator protocols use carrier RNA?
FAQ ID -2033
View
Can TopTaq Master Mix simply be stored at room temperature on the lab bench for up to 4 months?
FAQ ID -2052
View
Can the Type-it Mutation Detect PCR kit be used for multiplex PCR of fragments longer than 500 bp?
FAQ ID -2065
View
Can the Type-it Microsaltellite PCR Kit be used on cyclers with fast ramping rates?
FAQ ID -2062
View
Can 0.1 ml and 0.2 ml tubes used on the Rotor-Gene Q be labeled?
FAQ ID -2082
View
How many signal readings are taken during the real-time data acquisition on the Rotor-Gene Q?
FAQ ID -2083
View
Can membrane proteins be expressed with QIAgenes expression kits?
FAQ ID -2047
View
Can DNase digestion be performed on the RNeasy 96 plate during the RNA purification part of the Allprep DNA/RNA 96 procedure?
FAQ ID -2000
View
Does an internal control have to be included for each QIAsymphony Virus/Bacteria protocol run?
FAQ ID -2040
View
Do QIAsymphony Virus/Bacteria samples require pretreatment before they are loaded onto the instrument?
FAQ ID -2039
View
Does the centrifugal force have any effect on the kinetics of a PCR reaction on the Rotor-Gene Q?
FAQ ID -2081
View
How can a C-terminal 6xHis-tagged QIAgene be expressed?
FAQ ID -2041
View
How can full-length genes or individual domains of the QIAgenes Insect/Mammalia constructs be subcloned?
FAQ ID -2051
View
Does the Rotor-Gene Q software have the capacity to export data?
FAQ ID -2089
View
Can DNA yields be increased by prolonging incubation with proteinase K in Buffer ATL?
FAQ ID -2032
View
Can frozen samples be used with the MagAttract 96 cador Pathogen Kit?
FAQ ID -2070
View
How fast does the rotor spin during a run on the Rotor-Gene Q?
FAQ ID -2080
View
How is the log graph threshold value (Ct) for the Rotor-Gene Q determined?
FAQ ID -2088
View
How long can DNA converted with EpiTect Bisulfite Kits be stored before use in methylation specific PCR with the EpiTect MSP Kit?
FAQ ID -2002
View
How does the built-in QC control for complete bisulfite conversion of DNA work on PyroMark Q24?
FAQ ID -2095
View
How will running less than 96 samples with the MagAttract 96 cador Pathogen kit affect usage of plasticware?
FAQ ID -2071
View
If orders cannot be placed online, how can QIAgenes be ordered?
FAQ ID -2046
View
If new dyes or chemistries appear on the real-time PCR market, will Rotor-Gene Q be capable of running those dyes?
FAQ ID -2087
View
How long does a single run on the PyroMark Q24 take?
FAQ ID -2096
View
How much space does the PyroMark Q24 instrument need?
FAQ ID -2098
View
Does the Type-it Microsatellite PCR Kit always require an annealing temperature of 60°C, or can pre-optimized annealing temperatures for target-primer systems be used?
FAQ ID -2061
View
How accurate and reliable is PyroMark Q24 in mutation analysis?
FAQ ID -2094
View
Is there a charge for user training on the QIAsymphony SP System?
FAQ ID -1900
View
Is the QIAsymphony RNA Kit compatible with stabilized samples?
FAQ ID -1944
View
Is sample pretreatment required for purifying RNA on the QIAsymphony SP?
FAQ ID -1938
View
Is RNA co-purified with DNA using QIAsymphony DNA Tissue protocols?
FAQ ID -1920
View
What are the expected DNA yields from tissue processed on the QIAsymphony SP?
FAQ ID -1919
View
QuantiTect Primer Assays are bioinformatically validated, genomewide primer sets. What does “bioinformatically validated” mean?
FAQ ID -1982
View
What are the dimensions and weight of the QIAsymphony SP System?
FAQ ID -1901
View
What is the range of elution volumes on the QIAsymphony SP?
FAQ ID -1908
View
How many PCR reactions can be performed for different scales of miScript Primer Assays?
FAQ ID -1990
View
How many samples can be processed per kit or per reagent cartridge for RNA purification on the QIAsymphony SP?
FAQ ID -1940
View
How does QIAsymphony SP keep cellular samples in suspension prior to pipetting so that samples do not settle?
FAQ ID -1905
View
How efficient is DNA removal by a gDNA Eliminator 96 plate compared with DNase digestion on an RNeasy 96 plate?
FAQ ID -1994
View
How long can homogenates remain on the QIAsymphony SP without risk of RNA degradation?
FAQ ID -1941
View
How much sample can be used with the Allprep DNA/RNA 96 Kit?
FAQ ID -1997
View
How much sample can be used with the RNeasy Plus 96 Kit?
FAQ ID -1992
View
How much starting material can be used with the QIAsymphony DNA Mini Kit on the QIAsymphony SP?
FAQ ID -1917
View
What sample types can be used with the AllPrep DNA/RNA 96 Kit?
FAQ ID -1996
View
Which types and amounts of sample material can be processed for RNA extraction on the QIAsymphony SP?
FAQ ID -1937
View
What sample types can be used with the RNeasy Plus 96 Kit?
FAQ ID -1991
View
Will mitochondrial DNA also be isolated using the DNA Tissue protocols on the QIAsymphony SP?
FAQ ID -1921
View
What sample types should be processed with the High Content (HC) protocol for DNA extraction on the QIAsymphony SP?
FAQ ID -1918
View
Why does the Allprep DNA/RNA 96 Kit use Buffer RLT, whereas the AllPrep DNA/RNA Mini Kit uses Buffer RLT Plus?
FAQ ID -1999
View
Why is there no vacuum-only protocol for the RNeasy Plus 96 Kit?
FAQ ID -1993
View
What is the turn around time for custom miScript miRNA mimics, inhibitors and primer assays?
FAQ ID -1985
View
Is it possible to modify QIAsymphony protocols?
FAQ ID -1903
View
What are the starting volumes of homogenate for RNA extraction on the QIAsymphony SP?
FAQ ID -1939
View
What are the expected yields of total RNA isolated with the QIAsymphony SP?
FAQ ID -1942
View
What kind of molecules are miScript miRNA Inhibitors?
FAQ ID -1983
View
What sample input volumes can be used on the QIAsymphony SP?
FAQ ID -1907
View
What sample types can be processed using the tissue protocols for DNA extraction on the QIAsymphony SP?
FAQ ID -1915
View
What kind of molecules are miScript miRNA Mimics?
FAQ ID -1986
View
What RNA elution volumes are available with the QIAsymphony RNA Kit?
FAQ ID -1943
View
Do samples require pretreatment before loading onto the QIAsymphony SP for DNA extraction?
FAQ ID -1916
View
Do limiting primer concentrations need to be determined when using QuantiFast Multiplex PCR Kits?
FAQ ID -1976
View
Can uneven sample numbers be processed on the QIAsymphony SP module?
FAQ ID -1906
View
Does the QIAsymphony SP system generate run reports and/or log files?
FAQ ID -1911
View
Does HiPerFect work for the transfection of miScript miRNA mimics and inhibitors?
FAQ ID -1984
View
Do you have a protocol for the purification of archive-quality DNA from 0.5–20 mg paraffin-embedded tissue using Gentra Puregene Kits?
FAQ ID -1964
View
Do you have a protocol for the purification of archive-quality DNA from 1 ml of whole blood with the Gentra Puregene Tissue Kit?
FAQ ID -1953
View
Do you have a protocol for the purification of archive-quality DNA from 100 to 5 x 10e8 cultured cells using Gentra Puregene Kits?
FAQ ID -1960
View
Do you have a protocol for rapid purification of archive-quality DNA from up to 9 x 10e6 cells using Gentra Puregene Kits?
FAQ ID -1969
View
Can I program my own protocols for the QIAsymphony SP?
FAQ ID -1904
View
Are QuantiFast Multiplex PCR Kits available in trial sizes?
FAQ ID -1981
View
Are there any specific cleaning solutions recommended for the QIAsymphony SP?
FAQ ID -1913
View
Can 4-plex, real-time PCR be performed on the ABI PRISM 7000, 7700, or 7900 using the QuantiFast Multiplex PCR Kit?
FAQ ID -1978
View
Can the QIAsymphony RNA Kit be used to purify RNA from blood?
FAQ ID -1945
View
Can the QIAsymphony SP Module be connected to a laboratory information management system (LIMS)?
FAQ ID -1909
View
Can protocol runs on the QIAsymphony SP be interrupted and resumed?
FAQ ID -1912
View
Can the QuantiFast Multiplex PCR Kits be used on Roche LightCycler systems with TaqMan® probes?
FAQ ID -1979
View
Can the protocols for purification of small RNAs from cells in the appendices of the RNeasy Plus 96 Handbook also be used with tissue samples?
FAQ ID -1995
View
Do you have a protocol for the purification of archive-quality DNA from marine invertebrate tissue using Gentra Puregene Kits?
FAQ ID -1950
View
Do you have a protocol for the purification of archive-quality DNA from nematodes using Gentra Puregene Kits?
FAQ ID -1951
View
Do you have a protocol for the purification of archive-quality DNA from human dried blood using Gentra Puregene Kits?
FAQ ID -1959
View
Do you have a protocol for the purification of archive-quality DNA from Gram-negative bacteria using Gentra Puregene Kits?
FAQ ID -1975
View
Do you have a protocol for the purification of archive-quality DNA from Gram-positive bacteria using Gentra Puregene Kits?
FAQ ID -1946
View
Do you have a protocol for the purification of archive-quality DNA from hair roots using Gentra Puregene Kits?
FAQ ID -1947
View
Do you have a protocol for the purification of DNA from fruit flies?
FAQ ID -1970
View
Do you have a protocol for the purification of archive-quality DNA from tissue fixed in ethanol or formalin using Gentra Puregene Kits?
FAQ ID -1974
View
How can the QIAsymphony SP system be decontaminated?
FAQ ID -1914
View
Do you have a protocol for the purification of archive-quality DNA from umbilical cord blood using Gentra Puregene Kits?
FAQ ID -1958
View
Do you have a protocol for the purification of archive-quality DNA from buffy coat using Gentra Puregene Kits?
FAQ ID -1955
View
Do you have a protocol for the purification of archive-quality DNA from whole blood using Gentra Puregene Kits?
FAQ ID -1954
View
Do you have a protocol for the purification of archive-quality DNA from cerebrospinal fluid (CSF) using Gentra Puregene Kits?
FAQ ID -1967
View
Do you have a protocol for the purification of archive-quality DNA from Chlamydomonas using Gentra Puregene Kits?
FAQ ID -1968
View
Do you have a protocol for the purification of archive-quality DNA from blood smears using Gentra Puregene Kits?
FAQ ID -1948
View
Do you have a protocol for the purification of archive-quality DNA from fecal cells using Gentra Puregene Kits?
FAQ ID -1972
View
Do you have a protocol for the purification of archive-quality DNA from fish using Gentra Puregene Kits?
FAQ ID -1973
View
Do you have a protocol for the purification of archive-quality DNA from fungus using Gentra Puregene Kits?
FAQ ID -1971
View
Do you have a protocol for the purification of archive-quality DNA from buccal brushes using Gentra Puregene Kits?
FAQ ID -1965
View
Do you have a protocol for the purification of archive-quality DNA from Eimeria oocysts using Gentra Puregene Kits?
FAQ ID -1952
View
What effect does homogenization have on DNA yield and integrity when using AllPrep DNA/RNA Kits?
FAQ ID -1751
View
What is the fidelity of TopTaq DNA Polymerase?
FAQ ID -1739
View
Is TopTaq DNA Polymerase a hot-start polymerase?
FAQ ID -1748
View
Which nucleotide analogs can be used with TopTaq DNA Polymerase?
FAQ ID -1741
View
Why might Affymetrix GeneChip Mapping assays interfere with the REPLI-g FFPE kit?
FAQ ID -1755
View
What is the upper length of PCR products that can be amplified with TopTaq DNA Polymerase and Master Mix Kits?
FAQ ID -1738
View
What is the shelf-life of TopTaq DNA Polymerase and Master Mix?
FAQ ID -1743
View
What is the principle of the REPLI-g FFPE Kit?
FAQ ID -1753
View
What is the largest plasmid size that can be purified using the QuickLyse Miniprep Kit?
FAQ ID -1752
View
What is the minimum amount of starting material that can be used with the RNeasy Plus Micro and AllPrep DNA/RNA Micro Kit?
FAQ ID -1750
View
Can small miRNA-containing RNA fractions be separated from large RNAs using the miRNeasy FFPE Kit?
FAQ ID -1737
View
What is the recommended sample size for use with the REPLI-g FFPE Kit?
FAQ ID -1756
View
Can miRNA and other RNAs smaller than 200 nucleotides be isolated with the AllPrep DNA/RNA Micro Kit or RNeasy Plus Micro Kit?
FAQ ID -1749
View
Are there problems with the default threshold setting on the Applied Biosystems 7500 when using QuantiFast Probe PCR Kits?
FAQ ID -1702
View
Can TA/UA cloning be done with PCR products amplified with TopTaq DNA Polmyerase and TopTaq Master Mix Kits?
FAQ ID -1740
View
At which step in the REPLI-g FFPE protocol should PicoGreen quantification be done?
FAQ ID -1758
View
Have QuantiTect Primer Assays been tested with QuantiFast SYBR Green PCR Kits on the Mastercycler ep realplex?
FAQ ID -1714
View
How can foaming of Buffer RLT Plus lysates be avoided when using RNeasy Plus Micro and Mini Kits, or the Allprep DNA/RNA Mini Kit?
FAQ ID -1734
View
Do you have protocols for sample processing on the Autopure LS instrument?
FAQ ID -1759
View
Can the REPLI-g FFPE procedure restore DNA derived from FFPE tissue samples in the original order?
FAQ ID -1754
View
Can the REPLI-g FFPE Kit also be used for formalin-fixed, ethanol preserved samples?
FAQ ID -1760
View
Do you have a protocol for the isolation of total RNA from paraffin-embedded sections on the Biorobot M48?
FAQ ID -1733
View
Does TopTaq Stabilizer of the TopTaq DNA Polymerase and Master Mix Kits interfere with downstream applications?
FAQ ID -1744
View
Do you have a forensic post-PCR purification protocol to purify double-stranded DNA fragments from PCR reactions?
FAQ ID -1761
View
Do CoralLoad dyes supplied in various QIAGEN PCR Kits interfere with downstream applications?
FAQ ID -1745
View
Is FlexiTube GeneSolution available for other scales than the 1 nmol scale?
FAQ ID -1666
View
What buffer should be used to dilute cDNA made using the miScript Reverse Transcription Kit?
FAQ ID -1601
View
What is the binding capacity of the Ni-NTA Superflow Cartridges?
FAQ ID -1603
View
Is it necessary to clean up cDNA prepared with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1610
View
Is the master mix of QuantiFast Kits for real-time PCR aliquoted into several tubes to prevent cross-contamination?
FAQ ID -1697
View
Is the QuantiTect Whole Transcriptome Kit suitable for miRNA amplification?
FAQ ID -1609
View
Is sequence information for HP Validated siRNAs provided in the FlexiTube format?
FAQ ID -1665
View
Is it necessary to include a genomic DNA removal step with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1614
View
Which siRNA controls are available in FlexiTube format?
FAQ ID -1661
View
Where can I find background information and literature on Whole Genome Amplification with REPLI-g Kits?
FAQ ID -1690
View
Why is there DNA in the no-template (negative) control when using the QuantiTect Whole Transcriptome Kit?
FAQ ID -1620
View
What is the composition of Buffer PE?
FAQ ID -1691
View
What is the maximum volume of RNA in solution that can be used with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1616
View
Can the Ni-NTA Superflow Cartridges and/or Strep-Tactin Cartridges be connected in series?
FAQ ID -1606
View
Can the Ni-NTA and Strep-Tactin Superflow Cartridges be reused?
FAQ ID -1607
View
Can I store cDNA generated by the miScript System?
FAQ ID -1602
View
Can the QuantiTect Whole Transcriptome Kit be used for amplification of RNA from LCM samples?
FAQ ID -1612
View
Does the QuantiTect Whole Transcriptome Kit work with RNA purified from bacteria, yeast, or plants?
FAQ ID -1615
View
Can T-Script® enzyme of the QuantiTect Whole Transcriptome Kit be substituted by Quantiscript Reverse Transcriptase?
FAQ ID -1617
View
Can the QuantiTect Whole Transcriptome reaction be stopped after the reverse transcription step to proceed with the ligation and amplification steps at a later time?
FAQ ID -1618
View
Are FlexiTube siRNA and FlexiTube GeneSolution also available for rat siRNAs?
FAQ ID -1664
View
Can I still reorder siRNA that has been removed from the GeneGlobe data base?
FAQ ID -1668
View
Can amplification with the QuantiTect Whole Transcriptome Kit be extended to more than 8 hours to improve cDNA yields?
FAQ ID -1608
View
Can FlexiTube siRNA or FlexiTube GeneSolution products be ordered in solution?
FAQ ID -1667
View
Can I select HP Validated and HP GenomeWide siRNAs for FlexiTube?
FAQ ID -1658
View
Can cDNA prepared with the QuantiTect Whole Transcriptome Kit be reampliied using REPLI-g Kits?
FAQ ID -1611
View
How comparable is CoralLoad gel loading dye contained in various QIAGEN PCR Kits to Sigma Red?
FAQ ID -1644
View
How fast is the 6xHis-tagged protein purification process using Ni-NTA Superflow Cartridges?
FAQ ID -1605
View
How long is cDNA generated with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1613
View
How long can I archive cDNA generated with the QuantiTect Whole Transcriptome Kit?
FAQ ID -1623
View
Have you observed co-amplification of genomic DNA from RNA templates used in the QuantiTect Whole Transcriptome Procedure?
FAQ ID -1619
View
Do I need to resuspend FlexiTube siRNAs in siRNA Resuspension buffer?
FAQ ID -1659
View
Do you have a protocol for purification of total RNA from plant latex?
FAQ ID -1694
View
Do I need to use the miScript Universal Primer for detection of mRNA?
FAQ ID -1600
View
Do you offer a QuantiFast Kit for one-step RT-PCR?
FAQ ID -1695
View
Do you have a protocol for purification of total RNA from plant tissue with the RNeasy Lipid Tissue Mini Kit?
FAQ ID -1693
View
How many samples can be processed with the QIAsymphony DNA Mini Kit and the Virus Blood 200 protocol?
FAQ ID -2159
View
How often can the Strep-Tactin Superflow Plus resin be reused?
FAQ ID -2169
View
If housekeeping and target genes differ significantly in abundance, will they both be amplified with equal efficiency using the Rotor-Gene Multiplex PCR Kit?
FAQ ID -2129
View
If Vapor-Lock is used to overlay PCR reactions, which volume should be entered as reaction volume in the Rotor-Gene Q Software?
FAQ ID -2150
View
Is it normal that the binding capacity of GST-resins decreases with flow rate?
FAQ ID -2174
View
Is it possible to purify a GST-tagged protein under denaturing conditions using Glutathione Superflow?
FAQ ID -2172
View
Why is EvaGreen instead of SYBR Green used as fluorescent dye in the Type-it HRM PCR Kit?
FAQ ID -2196
View
Why is the initial activation step different for Rotor-Gene Probe, SYBR Green and Multiplex Kits?
FAQ ID -2118
View
Why is a 2-step (and not a 3-step) cycling protocol recommended for Rotor-Gene SYBR Green Kits?
FAQ ID -2122
View
Why do I need normalization using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2198
View
Why is there an asterisk for my miScript Primer Assays?
FAQ ID -2176
View
Will Rotor-Gene Kits also work on the Rotor-Gene 6000 and 3000 cyclers?
FAQ ID -2121
View
Will Uracil-N-Glycosylase (UNG) completely remove contaminating amplicons when using QuantiTect +UNG Kits?
FAQ ID -2139
View
Will QuantiTect Primer Assays work with Rotor-Gene SYBR Green Kits using an annealing step at 60ºC?
FAQ ID -2124
View
What is miRNA*? Do I need to consider this in my profiling experiment?
FAQ ID -2179
View
What are the main differences between Rotor-Gene and QuantiTect or QuantiFast PCR Kits?
FAQ ID -2119
View
What are possible reasons for reduced DNA yields with REPLI-g Kits?
FAQ ID -2148
View
What is the residuals plot in the Rotor-Gene ScreenClust HRM Software?
FAQ ID -2199
View
What is the target nucleic acid for the Virus Blood 200 Protocol on the QIAsymphony?
FAQ ID -2158
View
What is the minimum number of assays that can be ordered on QuantiTect Primer Assay Plates?
FAQ ID -2109
View
What is the detection limit of the Rotor-Gene and QuantiFast Multiplex RT-PCR Kits?
FAQ ID -2144
View
What is the maximum number of targets that can be amplified per reaction with the QuantiFast Multiplex RT-PCR Kit?
FAQ ID -2145
View
How should DNA isolated on the QIAsymphony be stored?
FAQ ID -2165
View
How should samples be stored before purification using the Virus Blood 200 Protocol on the QIAsymphony?
FAQ ID -2157
View
How pure should the chloroform used with the RNeasy Microarray Tissue Mini Kit be?
FAQ ID -2193
View
Why should I use the RNeasy Microarray Tissue Mini Kit when purifying RNA for microarray analysis?
FAQ ID -2188
View
Which is the best condition for eluting GST-tagged proteins from Glutathione Superflow?
FAQ ID -2173
View
Which elution volumes can be selected using the QIAsymphony Virus Blood 200 protocol?
FAQ ID -2160
View
What sample volumes can be used with the Virus Blood 200 Protocol on the QIAsymphony?
FAQ ID -2156
View
Which plate formats are offered for QuantiTect Primer Assay Plates?
FAQ ID -2106
View
What species are QuantiTect Primer Assay Plates offered for?
FAQ ID -2110
View
Why are the denaturation and annealing/extension times for QuantiFast Multiplex RT-PCR Kits much shorter than those for QuantiFast Multiplex PCR Kits?
FAQ ID -2147
View
Which sample types can be processed using the QIAsymphony Virus Blood 200 protocol?
FAQ ID -2154
View
How many reactions can be performed per well of the QuantiTect Primer Assay Plates?
FAQ ID -2107
View
Can I use the RNeasy Microarray Tissue Mini Kit for all tissue types?
FAQ ID -2190
View
Can assays for 20 and 100 reactions be ordered on the same QuantiTect Primer Assay Plate?
FAQ ID -2108
View
Can Glutathione Superflow resin be reused?
FAQ ID -2171
View
Can Uracil-N-Glycosylase (UNG) be added to the QuantiTect PCR Master Mix and be stored for later use?
FAQ ID -2138
View
Can QuantiTect Primer Assay Plates be ordered offline via e-mail or fax?
FAQ ID -2112
View
Are there any changes in the procedure using the Strep-Tactin Superflow Plus resin with the higher capacity over the standard matrix?
FAQ ID -2168
View
Can Uracil-N-Glycosylase (UNG) be purchased separately, independent of QuantiTect PCR Kits?
FAQ ID -2134
View
Are probes other than TaqMan® probes, such as FRET probes, compatible with fast cycling with the Rotor-Gene Multiplex PCR Kit?
FAQ ID -2130
View
Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with the Rotor-Gene Multiplex PCR Kit?
FAQ ID -2131
View
Can the Rotor-Gene Multiplex RT-PCR Kit be used on other cyclers?
FAQ ID -2142
View
Can Uracil-N-Glycosylase (UNG) be used with a one-step RT-PCR kit, such as the QuantiTect Probe RT-PCR Kit?
FAQ ID -2135
View
Can RNA isolated with the RNeasy Microarray Tissue Mini Kit also be used in downstream applications other than microarray analysis?
FAQ ID -2191
View
Are Rotor-Gene Kits compatible with reaction setup using the QIAgility instrument?
FAQ ID -2116
View
Are QuantiTect PCR +UNG Kits available in bulk format (e.g., 25 ml master mix)?
FAQ ID -2136
View
Are the layouts for both 96- and 384-well QuantiTect Primer Assay Plates visible when ordering online?
FAQ ID -2111
View
Are TaqMan® Gene Expression Assays from Applied Biosystems compatible with Rotor-Gene Probe Kits?
FAQ ID -2126
View
How does template quality influence the results with the Type-it HRM PCR Kit?
FAQ ID -2197
View
How does magnetic-particle carry-over in QIAsymphony eluates need to be handled?
FAQ ID -2166
View
Do you have protocols for software setup on various PCR platforms using QuantiFast Probe RT-PCR Kits?
FAQ ID -2105
View
How does the RNeasy Microarray Tissue Mini Kit prevent traces of phenol from negatively affecting the reverse-transcription step in microarray experiments?
FAQ ID -2189
View
How do Rotor-Gene Probe Kits compare with QuantiFast Probe Kits?
FAQ ID -2125
View
How many elution steps are recommended when using Glutathione Superflow resin?
FAQ ID -2175
View
How is the RNeasy Microarray Tissue Mini Kit different from the classic RNeasy Mini Kit?
FAQ ID -2192
View
Do you have a protocol for Rotor-Gene software setup for the Rotor-Gene SYBR Green PCR and RT-PCR Kits?
FAQ ID -2104
View
Does carrier RNA have to be added using the QIAsymphony Virus Blood 200 Protocol?
FAQ ID -2163
View
Does an internal control have to be used with the QIAsymphony Virus Blood 200 Protocol?
FAQ ID -2162
View
Can Vapor-Lock be used with samples processed on the QIAgility?
FAQ ID -2153
View
Do QuantiTect Primer Assays also work with Rotor-Gene SYBR Green PCR Kits?
FAQ ID -2123
View
Do the master mixes in Rotor-Gene Kits contain dUTP to allow UNG pretreatment?
FAQ ID -2117
View
Do limiting primer concentrations need to be determined when using the Rotor-Gene Multiplex PCR Kit?
FAQ ID -2128
View
What main input voltage is required for the QIAxcel Analyzer?
FAQ ID -1821
View
What is the minimum sample volume required for the QIAxcel System and QIAxcel Advanced?
FAQ ID -1852
View
Is there a quality difference between CompactPrep Plasmid Mega and Giga and the CompactPrep Plasmid Midi and Maxi Kits?
FAQ ID -1882
View
The Gel Cartridge is installed and all status indicators for the QIAxcel System are good but when clicking the "RUN" button, nothing happens. The green highlight goes down the Sequence?
FAQ ID -1850
View
Why does the copy function not apply relative to migration time and normalized area values after loading the DNA size marker table and adding the alignment marker peaks on the QIAxcel System?
FAQ ID -1832
View
What can be done if lower and upper alignment markers used with the QIAxcel System or QIAxcel Advanced are not aligned correctly?
FAQ ID -1834
View
Why are there no peaks in all channels during a run with the QIAxcel System or QIAxcel Advanced??
FAQ ID -1847
View
Why do DNA samples appear in only a few channels when the alignment marker is working using the QIAxcel System or QIAxcel Advanced?
FAQ ID -1844
View
Why does the Power LED not function with the QIAxcel or QIAxcel Advanced instrument plugged in and the main power switch turned on?
FAQ ID -1851
View
Can a QIAxcel DNA Fast Analysis Cartridge be run on a QIAxcel System?
FAQ ID -1828
View
Can customers use their own DNA size marker with the QIAxcel System and if yes, what concentration should be used?
FAQ ID -1841
View
Can data be saved at once with the QIAxcel System after analysis of all samples in a 96-well PCR plate?
FAQ ID -1861
View
How can a positive threshold be set when running the QIAxcel System?
FAQ ID -1843
View
How many intensity calibration runs can be performed per Gel Cartridge on the QIAxcel System?
FAQ ID -1827
View
How long will the intensity calibration procedure on the QIAxcel System take?
FAQ ID -1826
View
Why does an intensity calibration of the gel cartridge for the QIAxcel System and QIAxcel Advanced need to be performed?
FAQ ID -1824
View
Will the QIAxcel System run using a green Data Review Software Key?
FAQ ID -1853
View
Why is 'NO COM' displayed on the QIAxcel Instrument Panel although the power is on and all connections are made to the QIAxcel instrument and computer?
FAQ ID -1848
View
Why is the alignment marker for the 1.8 kb fragment not visible when using the 25 bp/1.8 kb DNA Size Marker together with the 15 bp/400 bp marker on the QIAxcel System and QIAxcel Advanced?
FAQ ID -1835
View
When running RNA samples using the QIAxcel System or QIAxcel Advanced, are there any special things to consider?
FAQ ID -1842
View
What to do if alignment marker and samples are migrating too slowly and the upper alignment marker does not appear on the gel- and/or electropherogram view with the QIAxcel System or QIAxcel Advanced?
FAQ ID -1833
View
Why are PCR bands relatively weak and "smearing" but the alignment marker bands are sharp when running samples on the QIAxcel System and QIAxcel Advanced?
FAQ ID -1837
View
When should an intensity calibration be re-performed on the QIAxcel System?
FAQ ID -1825
View
When should the N2 bottle used with the QIAxcel System be changed?
FAQ ID -1830
View
Can the QIAxcel software be loaded to another PC to view the data generated with the QIAxcel System?
FAQ ID -1855
View
Can the QIAxcel System or QIAxcel Advanced be connected to a network?
FAQ ID -1862
View
How can a clogged capillary channel be identified when running samples on the QIAxcel System or QIAxcel Advanced?
FAQ ID -1836
View
Can multiple QIAxcel Systems be run from one computer?
FAQ ID -1860
View
If a different DNA size and/or alignment marker is to be used with the QIAxcel System or QIAxcel Advanced, does the Gel Cartridge need to be recalibrated?
FAQ ID -1858
View
If a different method is used with the QIAxcel System or QIAxcel Advanced, does a new reference marker table need to be created?
FAQ ID -1857
View
How often should the alignment marker for the QIAxcel System be changed?
FAQ ID -1839
View
How often should the solutions in the Buffer Tray for the QIAxcel System or QIAxcel Advanced be replaced?
FAQ ID -1840
View
In the results table generated with the QIAxcel System, what does "normalized area" mean?
FAQ ID -1854
View
How can drying out of the gel cartridge tips used on the QIAxcel System and QIAxcel Advanced be prevented?
FAQ ID -1823
View
If the M500 method is used with the QIAxcel System, does a new DNA reference marker table have to be created?
FAQ ID -1856
View
How can any clogged capillaries be cleared for use with the QIAxcel System and QIAxcel Advanced?
FAQ ID -1838
View
What is the composition of buffer QBT?
FAQ ID -411
View
What is the composition of buffer QC?
FAQ ID -412
View
What is the composition of buffer P3?
FAQ ID -418
View
What is the composition of buffer QF?
FAQ ID -413
View
What is the composition of buffer TE?
FAQ ID -416
View
What is the composition of buffer QN?
FAQ ID -414
View
What is the composition of buffer OCD?
FAQ ID -423
View
What is the composition of buffer FWB2?
FAQ ID -417
View
What is the composition of buffer OBB?
FAQ ID -421
View
What is the composition of buffer OW2?
FAQ ID -419
View
What is the composition of buffer OL2?
FAQ ID -424
View
What is the composition of buffer OCL?
FAQ ID -422
View
Can RNAi experiments be performed in Drosophila?
FAQ ID -436
View
What is the size, charge and isoelectric point of the DHFR protein in the pQE vectors?
FAQ ID -470
View
Can the QIAamp MinElute Virus Kits be used to extract viral nucleic acid from cell culture supernatant?
FAQ ID -472
View
Has RNAi been successful using siRNA in Zebrafish and Xenopus?
FAQ ID -400
View
How is the RNeasy Lipid Tissue Mini Kit different from the RNeasy Mini Kit?
FAQ ID -468
View
How do I calculate the percentage of silencing with real-time RT-PCR for siRNA?
FAQ ID -498
View
Are Northern Blots sensitive enough to detect siRNA-induced gene silencing?
FAQ ID -403
View
Can QIAquick Kits be used to clean up RNA samples?
FAQ ID -490
View
Are HEPES, MOPS and PIPES buffers compatible with QIAGEN's PhosphoProtein Purification kit?
FAQ ID -482
View
Can Ni-NTA resins be used to purify protein with an internal His-tag?
FAQ ID -496
View
What is the composition of buffer STE?
FAQ ID -415
View
Can I use the RNeasy MinElute Cleanup Kit to clean up my in vitro transcription reaction?
FAQ ID -429
View
What is the composition of buffer OEB?
FAQ ID -420
View
Why are the centrifugation speeds for the QIAamp DNA Mini kit at 6000 x g? Can I spin at full speed?
FAQ ID -474
View
Which kit should be used to extract RNA from adipose tissue, brain, and other fatty animal tissues?
FAQ ID -467
View
Why is the pQE DNA provided in QIAexpress Kits blue in color?
FAQ ID -487
View
Why is carrier RNA used during the isolation of gDNA from microdissected samples with the QIAamp DNA Micro Kit?
FAQ ID -473
View
What is the minimum elution volume when using the RNeasy MinElute Cleanup Kit?
FAQ ID -432
View
What is the minimum elution volume when using the RNeasy Micro Kit?
FAQ ID -428
View
What is the smallest sample size that can be used with RNeasy Mini Kits?
FAQ ID -485
View
What is the maximum amount of starting material that can be processed with the RNeasy Micro Kit?
FAQ ID -426
View
What detection reagents (reporter fluorophores) do you recommend for the LiquiChip System?
FAQ ID -480
View
What is a homogeneous LiquiChip assay?
FAQ ID -476
View
What are the excitation and emission wavelengths of the reporter and classification lasers of the LiquiChip Instrument?
FAQ ID -479
View
What is the binding capacity of the RNeasy Micro Kit column?
FAQ ID -427
View
What is the composition and size of the LiquiChip Beads?
FAQ ID -475
View
What is the binding capacity of the RNeasy MinElute Cleanup Kit column?
FAQ ID -430
View
Is dihydrofolate reductase (DHFR) immunogenic?
FAQ ID -471
View
What are some of the assays that are adaptable to the LiquiChip System?
FAQ ID -477
View
My QuantiTect Master Mix did not freeze at degrees. Is it still ok to use?
-20
View
Will REPLI-g work at high temperatures?
FAQ ID -656
View
What is the run time for the BioSprint 96 DNA Blood protocol on the BioSprint 96 instrument?
FAQ ID -622
View
The Multiflow Dispense Head on my BioRobot is not dispensing the correct amount of liquid. What can I do?
FAQ ID -652
View
What is the smallest amount of DNA I can put into a 50 ul REPLI-g reaction?
FAQ ID -663
View
What is the shelf-life of my QIAGEN Kit?
FAQ ID -651
View
What is the recommended incubation time to stabilize tissue RNA in RNAprotect?
FAQ ID -647
View
What do you mean by “Quantification of locus representation” in REPLI-g WGA product?
FAQ ID -698
View
What is the average amount of DNA and RNA present in 1 ml normal serum?
FAQ ID -635
View
What is REPLI-g whole genome amplification?
FAQ ID -654
View
What is a QIAshredder? Is it sufficient for complete disruption and homogenization of my tissue sample?
FAQ ID -631
View
What do you recommend for the cleanup of genomic DNA (gDNA)?
FAQ ID -618
View
What is the expected yield of genomic DNA isolated from bacteria with the DNeasy Blood & Tissue or QIAamp DNA Mini Kit?
FAQ ID -632
View
What is the biggest protein that can be synthesized with the EasyXpress Protein Synthesis System?
FAQ ID -601
View
What is the length of the MDA whole genome amplification product?
FAQ ID -690
View
What 96-well plates do you recommend for use with the LiquiChip Workstation?
FAQ ID -630
View
Is it possible to perform laser microdissection with RNAprotect-stabilized tissue?
FAQ ID -608
View
Is it possible to synthesize membrane proteins using the EasyXpress Protein Synthesis Kit?
FAQ ID -602
View
What are Elution Microtube adapters and Microplate adapters used in the DirectPrep 96 Miniprep Kit, and how can I order them?
FAQ ID -646
View
What are the differences between MDA and DOP/PEP methods of Whole Genome Amplification?
FAQ ID -665
View
What are the advantages of REPLI-g over conventional DNA sample processing and amplification methods?
FAQ ID -686
View
Why do I get amplification in a negative control DNA tube using the REPLI-g Kit for WGA?
FAQ ID -675
View
Why does the QIAamp DNA Mini Tissue Protocol require both ATL and AL buffer, while the Blood Protocol only uses AL?
FAQ ID -633
View
Why do I sometimes get light blue colonies when using the QIAGEN PCR Cloning Kit?
FAQ ID -603
View
Why did you choose MAPK1 as a positive control in the QIAGEN RNAi Human/Mouse Control Kit?
FAQ ID -604
View
Why do you recommend using Triton X for the purification of 6xHis-tagged protein?
-100
View
Will the PhosphoSerine antibody recognize Serine residues if the neighboring residues are Leucine or Isoleucine?
FAQ ID -644
View
Will the QIAquick PCR Purification Kit remove sufficient SYBR Green from real-time PCR reactions to allow sequencing?
FAQ ID -637
View
Will TA cloning work with REPLI-g WGA product?
FAQ ID -672
View
Will DNA from chromosomes collected in TE with a drop of mineral oil work with REPLI-g amplification?
FAQ ID -664
View
What is the stability of the REPLI-g MDA product?
FAQ ID -693
View
When delivering liquid to a 96-well plate using the MultiFlow Dispenser Head on my BioRobot , one or more rows in the plate contains less liquid per well than specified by the protocol. What is going wrong?
FAQ ID -653
View
Where should I add a dye-label modification on the siRNA?
FAQ ID -648
View
What types of materials from environmental samples could interfere with WGA?
FAQ ID -666
View
What size is the smallest protein that can be synthesized with the EasyXpress Protein Synthesis Kit?
FAQ ID -600
View
Can I stabilize RNA in bacteria grown on solid media or substrate using the RNAprotect Bacteria Reagent?
FAQ ID -615
View
Can I run less than 15 samples on the BioSprint 15 instrument?
FAQ ID -621
View
Can I re-amplify whole genome amplification product?
FAQ ID -670
View
Can I use another lysis buffer than the one supplied in the PhophoProtein Purification Kit?
FAQ ID -645
View
Can the QIAshredder Maxi Spin Columns from the DNeasy Plant Maxi Kit be used for RNeasy samples?
FAQ ID -616
View
Do you have an off-the shelf diagnostics kit for WGA?
FAQ ID -678
View
Can the RNase-Free DNase Set be used for DNase digestions of RNA in solution?
FAQ ID -619
View
Can the computer for the LiquiChip Workstation be networked?
FAQ ID -629
View
Are there components in biological samples that can inhibit the REPLI-g reaction?
FAQ ID -667
View
Are the BioSprint 15/96 instruments stand-alone workstations?
FAQ ID -620
View
Are samples that are contaminated with high molecular weight DNA (e.g. from a paramedic or police officer) a problem with REPLI-g amplification?
FAQ ID -683
View
Are QIAGEN EZ Competent Cells of the QIAGEN PCR Cloning Kit dam+ or dam-?
FAQ ID -638
View
Can BAC DNA prepared with the R.E.A.L. Prep 96 Plasmid Kit be used for high-throughput sequencing?
FAQ ID -643
View
Can I do whole genome amplification from brain tissue using REPLI-g?
FAQ ID -669
View
Can FTA paper for dried blood spots be used with REPLI-g?
FAQ ID -685
View
Can I do whole genome amplification from mitochondrial DNA using REPLI-g?
FAQ ID -668
View
Can I amplify from plasma or serum samples using the REPLI-g Mini/Midi kits?
FAQ ID -671
View
How can I minimize degradation of RNA from my pancreas sample?
FAQ ID -624
View
How can I quantify the amount of REPLI-g DNA I have amplified?
FAQ ID -694
View
How should I store the REPLI-g amplification product?
FAQ ID -692
View
Is 2 kb the minimal gDNA fragment size for REPLI-g Whole Genome Amplification?
FAQ ID -682
View
How long can I store bacterial culture stabilized in RNAprotect Bacteria Reagent?
FAQ ID -614
View
Do I need to clean up the REPLI-g amplification product for downstream analysis?
FAQ ID -691
View
Does REPLI-g technology for whole genome amplification work with paraffin embedded samples?
FAQ ID -673
View
Do you have a kit for RNA isolation from any kind of sample type?
FAQ ID -627
View
How can I improve my RNA yield from liver sample when using RNeasy Kits?
FAQ ID -623
View
How can I determine the quality of my REPLI-g amplified products?
FAQ ID -695
View
Do you know of anything in the buffers/DNA sample that can cause bias in REPLI-g WGA?
FAQ ID -684
View
What is the composition of the QIAGEN 10x PCR Buffer in Taq- and HotStarTaq DNA Polymerase Kits?
FAQ ID -606
View
Is it possible to use less than 1 mg of protein with the PhosphoProtein Purification Kit?
FAQ ID -599
View
I want to start with gene silencing (RNAi) experiments. Do you have informational literature?
FAQ ID -580
View
What can I do when the DNA pellet prepared with QIAGEN Plasmid Kits has been overdried?
FAQ ID -572
View
What is a quenched FRET assay?
FAQ ID -542
View
Is it possible to use a QIAzol lysate for the isolation of RNA from tissue on the BioRobot EZ1?
FAQ ID -573
View
What do you recommend for isolating RNA from more than 100 mg of plant tissue?
FAQ ID -570
View
What happens if I spin my lysate on the RNeasy Spin Columns at maximum speed?
FAQ ID -514
View
What has to be done to a RNA sample before loading it onto an Agilent Bioanalyzer?
FAQ ID -528
View
How should I dissolve the lyophilized Anti-His and Tag Antibodies?
-100
View
Is it possible to isolate single stranded DNA (ssDNA) with the QIAEX II Kit from agarose or polyacrylamide gels?
FAQ ID -576
View
Is it possible to perform a UNG treatment when using QuantiTect kits?
FAQ ID -564
View
Is it possible to isolate DNA from bone marrow with the QIAamp DNA Blood Kits?
FAQ ID -563
View
Is it possible to clean up a methylation reaction containing bisulfite with QIAquick Cleanup Kits?
FAQ ID -519
View
Is it possible to isolate both RNA and recombinant 6xHis-tagged protein from the same sample?
FAQ ID -532
View
Why are samples centrifuged at 4°C after the addition of chloroform when using RNeasy Lipid Tissue Kits?
FAQ ID -533
View
Why are my realtime PCR amplification plots hook-shaped?
FAQ ID -587
View
How was it determined that the PhosphoSerine Antibody will bind phosphoserines only?
FAQ ID -579
View
If pQE40 is used to express a fusion protein with DHFR, can the DHFR be cleaved afterwards?
FAQ ID -513
View
How should I store cDNA produced using Omniscript Reverse Transcriptase?
FAQ ID -561
View
Which chloroform should I use for the RNeasy Lipid Tissue Kit?
FAQ ID -516
View
Will pancreas tissue swim on top of RNAprotect?
FAQ ID -536
View
Will the 2x QIAGEN Multiplex PCR Master Mix freeze at -20°C?
FAQ ID - 525
View
Why does my realtime PCR assay quality decrease over time?
FAQ ID -589
View
Why do replicates in real-time PCR have different plateau heights?
FAQ ID -539
View
What is the longest fragment that was cloned with the pQE UA Cloning Vector?
-30
View
What is the difference between BSA-free and BSA-containing RGS-His Antibody?
FAQ ID -569
View
What is the composition of Buffer ER? Is it available separately?
FAQ ID -571
View
What is the composition of the siRNA resuspension & annealing buffer?
FAQ ID -522
View
When using QIAzol Lysis Reagent for RNA extraction, I see a pinkish colored aqueous phase! Is it OK?
FAQ ID -534
View
Where can I find the public Smith-Waterman homology search tool that you refer to on your siRNA online design page?
FAQ ID -562
View
What reaction volume should I use with QuantiTect Kits?
FAQ ID -551
View
What is Tissue-Tek O.C.T., and what is it used for?
FAQ ID -530
View
What makes QIAGEN's 10x Taq and HotStarTaq DNA Polymerase PCR buffer superior?
FAQ ID -566
View
How many template copies can I detect using QuantiTect Kits in real-time PCR?
FAQ ID -543
View
Can I use RNAiFect for co-transfection of plasmid DNA and siRNA?
FAQ ID -515
View
Does DEPC harm RNA?
FAQ ID -529
View
Does endogenously expressed DAPase in human cells degrade proteins that are expressed in cell culture?
FAQ ID -527
View
Can Taq DNA Polymerase use RNA as a template, and generate false positives in "no-RT" controls?
FAQ ID -523
View
Can the MAPK1 control siRNA be used for rat cells?
FAQ ID -547
View
Are the columns of the MinElute Reaction Cleanup-, Gel Extraction-, and PCR Purification Kit identical?
FAQ ID -581
View
Are the columns of the QIAquick PCR Purification-, Gel Extraction-, and Nucleotide Removal Kit interchangeable?
FAQ ID -577
View
After using your U/A PCR Cloning Kit I can detect colonies, which are light blue, and not really white. Why is that?
FAQ ID -512
View
Can I make a master mix with QuantiProbes, primers, and all other reaction components and store it for later use?
FAQ ID -584
View
Can I shorten the activation time for the HotStarTaq DNA Polymerase?
FAQ ID -565
View
Can I homogenize samples in QIAzol using the QIAshredder?
FAQ ID -501
View
Do you offer QIAshredder spin columns for the RNeasy Midi/Maxi Kit format? If not, what is the alternative?
FAQ ID -560
View
How can I avoid primer-dimer formation during PCR amplification?
FAQ ID -544
View
Does QIAGEN sell 6xHis-tagged proteins besides the 6xHis Protein Ladder?
FAQ ID -594
View
Do you provide control proteins for use with the PhosphoProtein Purification Kit?
FAQ ID -596
View
How long should the amplicon be if I am using QuantiTect SYBR Green detection?
FAQ ID -553
View
How is an antibody epitope determined?
FAQ ID -593
View
Do I need to calibrate my real-time cycler if I want to use Yakima Yellow?
FAQ ID -540
View
How can I tell if I have primer-dimers in my PCR reaction?
FAQ ID -552
View
Do we recommend pooling siRNA sequences?
FAQ ID -517
View
Do I have to remove the oil from my PCR reaction before using the QIAquick or MinElute PCR Purification Kit?
FAQ ID -575
View
Does the His-tag interfere with the purification procedure of the QIAGEN PhosphoProtein Purification Kit?
FAQ ID -598
View
Does the use of MAPK1 as a control in siRNA transfection experiments have secondary effects on the cell culture?
FAQ ID -548
View
Do you have a protocol to isolate RNA from blood and bone marrow using the BioRobot EZ1?
FAQ ID -567
View
Do you have stability data for genomic DNA isolated with the QIAamp DNA Blood Mini Kit?
FAQ ID -518
View
Do you have a protocol for the removal of endotoxins from already purified plasmid DNA?
FAQ ID -500
View
Do you have a protocol for isolating RNA from yeast using RNeasy?
FAQ ID -535
View
Do you have any recommendations for using the PosphoProtein Kit with starting material other than cells?
FAQ ID -597
View
Do you have a protocol for isolation of genomic DNA from saliva using the DNeasy Blood & Tissue Kit?
FAQ ID -931
View
Do you have a protocol for the isolation of DNA from epithelial cells mixed with sperm cells?
FAQ ID -926
View
Do you have a protocol for the purification of DNA from rodent tail using the BioSprint System?
FAQ ID -979
View
Do you have a protocol for the isolation of DNA from tofu?
FAQ ID -932
View
Do you have a protocol for Streptokinase treatment of clotted blood?
FAQ ID -912
View
Do you have a protocol for purification of DNA from clotted blood using the FlexiGene DNA Kit?
FAQ ID -973
View
Do you have a protocol for the isolation of RNA from leukocytes in milk?
FAQ ID -952
View
Do you have a protocol for the isolation of total nucleic acids from animal and human tissues on the BioRobot EZ1?
FAQ ID -975
View
Do you have a protocol for the isolation of total nucleic acids from animal and human cells on the BioRobot EZ1?
FAQ ID -974
View
Do you have a protocol for the isolation of total RNA from buccal swabs?
FAQ ID -956
View
Do you have a protocol for the isolation of viral DNA from stool?
FAQ ID -929
View
Do you have a protocol for the isolation of total RNA from plant tissue using QIAGEN-tips?
FAQ ID -949
View
Do you have a protocol for the isolation of total RNA from paraffin-embedded sections on the BioRobot EZ1?
FAQ ID -976
View
Do you have a protocol for the isolation of total RNA from plants using the RNeasy 96 Kit?
FAQ ID -951
View
Do you have a protocol for the isolation of DNA from soft tissues using the TissueLyser?
FAQ ID -924
View
Do you have a protocol for the isolation of genomic DNA from sperm?
FAQ ID -909
View
Do you have a protocol for the isolation of DNA from formalin-preserved stool samples?
FAQ ID -922
View
Do you have a protocol for the isolation of DNA from fungi using the BioRobot EZ1?
FAQ ID -992
View
Do you have a protocol for the isolation of genomic DNA from fungi?
FAQ ID -911
View
Do you have a protocol for the isolation of genomic DNA and/or proteins from fatty tissue treated with QIAzol?
FAQ ID -955
View
Do you have a protocol for the isolation of genomic DNA from frozen clotted blood?
FAQ ID -900. Test
View
Do you have a protocol for the preparation of RNAprotect preserved tissues for pathological studies?
FAQ ID -950
View
Do you have a protocol for the purification of DNA from cells using the BioSprint System?
FAQ ID -977
View
Do you have a protocol for the purification of DNA from dried blood spots using the BioSprint System?
FAQ ID -982
View
Do you have a protocol for isolation of genomic and plasmid DNA from cell cultures using QIAGEN Genomic-tips?
FAQ ID -902
View
Do you have a protocol for transfection of adherent cells with low concentrations of siRNA using RNAiFect Transfection Reagent?
FAQ ID -970
View
Do you have a protocol for the purification of DNA from buccal swabs using the BioSprint System?
FAQ ID -983
View
Do you have a protocol for GMO testing of food samples?
FAQ ID -921
View
Do you have a protocol for isolation of bacterial DNA from soil?
FAQ ID -923
View
Do you have a protocol for isolation of genomic and viral DNA from lymphocytes using QIAGEN Genomic-tips?
FAQ ID -901
View
Do you have a protocol for isolation of genomic DNA from saliva and mouthwash?
FAQ ID -917
View
Do you have a protocol for isolation of genomic DNA from dried blood spots using the QIAamp 96 DNA Blood Kit?
FAQ ID -919
View
Do you have a protocol for isolation of genomic DNA from insects?
FAQ ID -904
View
Do you have a protocol for detection of HBV DNA by PCR?
FAQ ID -915
View
Can the BioSprint instruments be placed in a cool room?
FAQ ID -938
View
Does QIAGEN sell a vector with a T7 promotor for use with the EasyXpress Protein Synthesis Insect Kit?
FAQ ID -937
View
Do you have a protocol for Acetyl Cysteine (NALC) treatment of viscous samples?
FAQ ID -913
View
Do you have a protocol for co-transfection of adherent cells with siRNA and plasmid DNA?
FAQ ID -969
View
Do you have a protocol for Cyanine 570, Cyanine 670, or biotin labeling of cDNA?
FAQ ID -959
View
Do you have a protocol for adding new reporter dyes to the ABI PRISM 7900 Sequence Detection System?
FAQ ID -965
View
Do you have a protocol for cleanup of non-radioactive DNA samples?
FAQ ID -945
View
Do you have a protocol for radioactive labelling of cDNA?
FAQ ID -960
View
Do you have a protocol for reverse transfection of adherent cells with siRNA in 384-well plates?
FAQ ID -971
View
Do you have a protocol for the isolation of genomic DNA from bone?
FAQ ID -908
View
Do you have a protocol for the isolation of viral RNA from stool?
FAQ ID -918
View
Do you have protocols for the isolation of genomic DNA from tissue using QIAGEN-tips 2500 (Mega) and 10000 (Giga)?
FAQ ID -907
View
Do you have protocols for multiple extractions of DNA fragments from agarose gels?
FAQ ID -944
View
Why is it not recommended to stabilize cells with RNAprotect?
FAQ ID -941
View
What do you suggest to prevent degradation of RNA isolated from tissue with high amounts of RNases using RNeasy?
FAQ ID -942
View
Do you have a protocol for transient transfection of Hela-S3 cells using PolyFect Transfection Reagent?
FAQ ID -997
View
Do you have a protocol for the detection of Bordetella pertussis DNA by PCR?
FAQ ID -914
View
Do you have a protocol for the isolation of DNA from buffy coat using the BioRobot EZ1?
FAQ ID -985
View
Do you have a protocol for the isolation of DNA from cultured cells using the BioRobot EZ1?
FAQ ID -990
View
Do you have a protocol for performing quantitative multiplex PCR on the LightCycler 2.0 system?
FAQ ID -966
View
Do you have a protocol for long-term, siRNA-mediated gene silencing using HiPerFect Transfection Reagent?
FAQ ID -972
View
Do you have a protocol for polyacrylamide gel analysis of oligonucleotides?
FAQ ID -961
View
Do you have a protocol for manual purification of 6xHis-tagged proteins expressed in E. coli using Ni-NTA Superflow?
FAQ ID -967
View
Do you have a protocol for purification of DNA from cultured animal cells using the DNeasy 96 Blood & Tissue Kit?
FAQ ID -934
View
Do you have a protocol for purification of DNA fragments from Cy3/Cy5 dye-label reactions?
FAQ ID -947
View
Do you have a protocol for purifying 2x96 PCR samples simultaneously with the QIAquick 96 PCR Purification Kit?
FAQ ID -935
View
Do you have a protocol for the purification of PCR products using the BioSprint System?
FAQ ID -984
View
Do you have a protocol for isolation of genomic DNA from plants and filamentous fungi using QIAGEN Genomic-tips?
FAQ ID -905
View
Do you have a protocol for isolation of genomic DNA from flies using QIAGEN Genomic-tips?
FAQ ID -903
View
Is it possible to transfect DNA into insect cell lines such as Drosophila melanogaster S2?
FAQ ID -397
View
Is it possible to isolate free genomic DNA using the QIAamp UltraSens Virus Kit?
FAQ ID -358
View
Is QIAGEN carrier RNA sold separately?
FAQ ID -351
View
I accidentally added RNAprotect to my cells, and now the cells are difficult to pellet. What can I do?
FAQ ID -364
View
What are the calculated molecular weights of the TAGZyme enzymes?
FAQ ID -329
View
Is it possible to store TAGzyme enzymes at°C?
-80
View
Is Q-Solution required for PCR with QIAGEN's PCR kits?
FAQ ID -380
View
How do I know if my plasmid is a high- or low copy number type?
FAQ ID -350
View
How do I perform a DNA precipitation to concentrate my sample?
FAQ ID -305
View
How can QIAGEN Protease and Proteinase K be inactivated?
FAQ ID -315
View
How complete is the removal of DAPase in the TAGZyme process?
FAQ ID -319
View
How long is fluorescence detectable in cells after transfection with fluorescently labeled siRNAs?
FAQ ID -392
View
How many transfections can I perform with 20 nmol of HPP grade siRNA?
FAQ ID -367
View
How do I submit a siRNA order by telephone or online?
FAQ ID -399
View
What is the white insoluble precipitate in my resuspended plasmid DNA pellet?
FAQ ID -352
View
What kits does QIAGEN offer to extract RNA from whole blood?
FAQ ID -304
View
Why do I get genomic DNA contamination in my plasmid prep?
FAQ ID -353
View
Which primers can I use for sequencing pQE-expression vector constructs?
FAQ ID -343
View
What is the standard assay volume recommended for LiquiChip assays?
FAQ ID -330
View
What is the average molecular weight of a siRNA, and how do I convert uM to ug values?
FAQ ID -388
View
What are the dissociation constants for the Anti-His Antibodies?
FAQ ID -385
View
What is the lowest elution volume that can be used with QIAprecipitator Midi and Maxi Modules?
FAQ ID -307
View
What is the origin of replication and the plasmid copy number of the pQE vectors?
FAQ ID -338
View
What is the acceptable maximum g force for centrifugation of Xa Removal Resin?
FAQ ID -320
View
What is the composition of Buffer FG3?
FAQ ID -356
View
What is the composition of PBS?
FAQ ID -361
View
How can I keep my centrifuge tubes endotoxin-free?
FAQ ID -301
View
Can I use ethanol instead of isopropanol for DNA precipitation when using HiSpeed Plasmid Kits?
FAQ ID -354
View
303 - Can I use my own lysis buffer with the DNeasy Blood & Tissue or QIAamp DNA Mini Kit?
View
Can I store agarose gel slices containing DNA for gel extraction at a later point?
FAQ ID -313
View
Can I use a partial MinElute 96 UF plate?
FAQ ID -355
View
Can I use the RNeasy Mini Kit or RNeasy 96 Kit with fewer than 100 cells?
FAQ ID -372
View
Can I use water for elution from the QIAprecipitator of the HiSpeed Plasmid Kits?
FAQ ID -306
View
Are QIAprep and QIAquick Spin columns interchangeable?
FAQ ID -311
View
Are the QIAprecipitator Midi and Maxi Modules of the HiSpeed Plasmid Kits interchangeable?
FAQ ID -308
View
Apart from the antibody itself, does the Penta-His Biotin Conjugate solution contain any protein?
FAQ ID -359
View
Are QIAGEN monoclonal Anti His Antibodies glycosylated?
FAQ ID -334
View
Can I eliminate RNase A from buffer P1 for my plasmid preparation to obtain RNase-free DNA for in-vitro transcription?
FAQ ID -366
View
Can I perform in-vitro transcription with the pDrive Cloning Vector from the QIAGEN PCR Cloning Kit?
FAQ ID -332
View
Can Buffers N3 and P3 be used interchangeably?
FAQ ID -310
View
Do the QuantiTect SYBR Green Kits contain a stabilization reagent that inhibits the sensitivity of real-time PCR?
FAQ ID -328
View
How are siRNAs introduced into C. elegans?
FAQ ID -396
View
Do I need to anneal, deprotect or desalt my QIAGEN siRNA?
FAQ ID -398
View
Do pQE vectors contain the CAT gene?
FAQ ID -362
View
How can I improve DNA yields from very tough tissues using the DNeasy Blood & Tissue Kit or the QIAamp DNA Mini Kit?
FAQ ID -374
View
How can I improve the expression of proteins containing hydrophobic regions?
FAQ ID -339
View
How can I check if any residual proteins remain on the Ni-NTA Agarose matrix after elution?
FAQ ID -324
View
How can I express toxic protein in E. coli?
FAQ ID -373
View
Do freeze-thaw cycles affect the stability of the U-overhang on the pDrive Cloning Vector?
FAQ ID -323
View
Can RNAprotect Tissue Reagent be used to store previously isolated RNA?
FAQ ID -337
View
Can MinElute 96 UF PCR be used to purify PCR samples which contain mineral oil?
FAQ ID -360
View
Can siRNA silence bacterial genes?
FAQ ID -395
View
Does SYBR Green dye present in QuantiTect kits interfere with ethidium bromide staining?
FAQ ID -312
View
Does the 5x OneStep RT-PCR Buffer contain BSA?
FAQ ID -326
View
Do any of the buffers in the HotStarTaq DNA Polymerase Kit contain Triton?
FAQ ID -327
View
Do any of the kit components or the product packaging of the QIAamp 96 DNA Blood Kit contain latex?
FAQ ID -318
View
What are exo-resistant random hexamers used in the REPLI-g reaction?
FAQ ID -710
View
The Sigma Centrifuge makes a loud noise at low speeds which disappears at higher speeds, when using the 2x96-well plate rotor. What is the problem?
FAQ ID -776
View
Is RNAprotect Bacteria Reagent compatible with the RNeasy Mini Kit?
FAQ ID -797
View
What is the average DNA concentration obtained using the DirectPrep 96 Miniprep Kit?
FAQ ID -721
View
What are the expected average DNA yields from plant extractions on the BioSprint 96?
FAQ ID -771
View
What is the basic technology behind the Strep-tag Protein Purification System?
FAQ ID -740
View
What is the advantage of running an analytical gel with fractions of my plasmid preparation?
FAQ ID -769
View
What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?
FAQ ID -728
View
I bound an 11 kb DNA fragment to a QIAquick column; is it completely lost?
FAQ ID -756
View
If template abundance differs significantly between housekeeping and target gene, are both amplified equally efficient with the QuantiTect Multiplex Kits?
FAQ ID -718
View
How to quantitate DNA purified using the BioSprint 96?
FAQ ID -773
View
How to store DNA from forensic samples eluted into water on the EZ1 biorobot in TE buffer instead?
FAQ ID -734
View
I accidentally stored Buffer RDD of the RNase-Free DNase Set at°C. Will it still function?
-20
View
Is it possible to use LiquiChip beads for FACS applications?
FAQ ID -733
View
Is it possible to use the QuantiTect Reverse Transcription Kit with bacterial RNA?
FAQ ID -785
View
I ran my RNA out on an agarose gel and can see lots of bands similar to a ladder. Why?
FAQ ID -745
View
Is GFP excited and detected by the green laser of the LiquiChip System?
FAQ ID -737
View
Is it possible to buy the EZ Competent Cells of the QIAGEN PCR Cloning Plus Kit separately?
FAQ ID -763
View
Which Qproteome or Protein Fractionation Kits from QIAGEN are compatible with tissue samples?
FAQ ID -755
View
Why are some of my RNAprotect-stabilized samples frozen atºC while others are not?
ID -758
View
Where can I find info on compatible reporter dyes for use in real-time multiplex PCR using the QuantiTect Multiplex PCR Kits?
FAQ ID -719
View
Why are the QuantiTect and QuantiFast Multiplex PCR Kits limited to triplex real-time PCR on some cyclers?
FAQ ID -715
View
Why might my gene of interest drop out during WGA if it is not near a telomere or centromere?
FAQ ID -703
View
Will the random hexamers in the REPLI-g reaction interfere with downstream analysis?
FAQ ID -713
View
Why should DNA or cDNA targets be less than 250 bp long for real-time PCR?
FAQ ID-751
View
Why is my plasmid DNA yield low?
FAQ ID -768
View
What is the difference between Ni-NTA Agarose and Ni-NTA Superflow?
FAQ ID -764
View
What is the difference between QIAGEN Protease and QIAGEN Proteinase K provided in various QIAamp Kits?
FAQ ID -761
View
Why is there no detection of free reporter fluorescence in the LiquiChip System?
FAQ ID -732
View
What is the composition of buffer AE?
FAQ ID -730
View
What is the cell number range one can use with the FastLane Cell cDNA Kit for real-time RT-PCR?
FAQ ID -782
View
What is the composition of Buffer N3?
FAQ ID -767
View
What negative (unphosphorylated) and positive (phosphorylated) control proteins do you recommend for use with the PhosphoProtein Purification Kit?
FAQ ID -779
View
Where can I find a copy of the report files generated by running accessory protocols "Dilutor Delivery (monthly)" and "High-Speed Pipetting Sytem (monthly)" on my BioRobot 9604?
FAQ ID -766
View
What type of Sequencing Services does QIAGEN offer?
FAQ ID -781
View
What is the enzyme used in the REPLI-g reaction?
FAQ ID -704
View
What is the recipe for SOC medium?
FAQ ID -798
View
What is the general sensitivity level of the LiquiChip System?
FAQ ID -731
View
How should fluorescent labeled probes be stored?
FAQ ID -784
View
Can QIAGEN's Taq- and HotstarTaq DNA Polymerases be used for cycle sequencing?
FAQ ID -741
View
Can RNAprotect Tissue Reagent be used to stabilize tissue which has already been flash-frozen?
FAQ ID -757
View
Can I use the PhosphoProtein Purification Kit to purify phosphorylated peptides of around 1 kD size?
FAQ ID -778
View
Can lyophilized plant material be used for DNA extraction on the BioSprint 96?
FAQ ID -772
View
Can I use the QuantiTect Multiplex PCR Kits on the Roche LightCycler systems with TaqMan® probes or QuantiTect Assays?
FAQ ID -717
View
Can the REPLI-g WGA enzyme work with labeled dNTPs?
FAQ ID -706
View
Does the QIAamp DSP Virus Kit require the QIAvac 24 Plus, or can I use it with my own laboratory vacuum system?
FAQ ID -789
View
Can the MAPK1 siRNA of the RNAi Human/Mouse Starter Kit be purchased separately?
FAQ ID -744
View
Can the QIAprep Spin Miniprep Kit be used for isolating plasmid DNA from mammalian cells?
FAQ ID -795
View
Can the QIAvac 96 vacuum manifold be used with DirectPrep 96 plates?
FAQ ID -722
View
Are the buffers in the Ni-NTA Fast Start Kit the same as the ones for use with Ni-NTA purchased separately?
FAQ ID -791
View
Are the protocols from the BioSprint software compatible with the KingFisher software and vice versa?
FAQ ID -770
View
Are Buffer PB of the QIAquick PCR Purification Kit and Buffer QG of the QIAquick Gel Extraction Kit interchangeable?
FAQ ID -786
View
Any data on the fidelity of the REPLI-g MDA technique?
FAQ ID -707
View
Are Centromeres and Telomeres amplified using REPLI-g WGA?
FAQ ID -702
View
Can I use my own primers for REPLI-g WGA to amplify a specific chromosomal region?
FAQ ID -712
View
Can I use REPLI-g for SNP Genotyping?
FAQ ID -700
View
Can I do 4-plex real-time PCR, on the ABI PRISM 7000, 7700, or 7900 using the QuantiTect Multiplex PCR Kit?
FAQ ID -716
View
Can I purchase Phi29 DNA polymerase only?
FAQ ID -708
View
How can I get specific information and a quote for my individual Sequencing Service needs?
FAQ ID -792
View
How can one perform multiplex real-time PCR analysis using the LightCycler 2.0?
FAQ ID -780
View
How can I be sure that I am harvesting my induced bacterial culture at the best time point for protein expression?
FAQ ID -788
View
How can I get a certificate for quality assurance (ISO 13485 and ISO 9001) from QIAGEN?
FAQ ID -762
View
How do the FastLane Cell cDNA Kit and QuantiTect Reverse Transcription Kit eliminate genomic DNA contamination?
FAQ ID -783
View
How many cells can be used with the FastLane Cell cDNA Kit?
FAQ ID -796
View
Do you have an antibody for Western Blot Analysis of MAPK1 gene knockdown using your MAPK1 control siRNA?
FAQ ID -746
View
Do I have to switch off the laser of the LiquiChip Workstation overnight?
FAQ ID -736
View
Can frozen blood be used for whole genome amplification with REPLI-g?
FAQ ID -794
View
How many reporter dyes can be used in the same analysis using the LiquiChip Workstation?
FAQ ID -738
View
How much DNA is obtained in the average PCR reaction?
FAQ ID -750
View
Do I need to determine limiting primer concentrations with the QuantiTect Multiplex PCR Kits?
FAQ ID -714
View
Do I need to have EDTA in the buffer in which I am going to store my isolated genomic DNA?
FAQ ID -754
View
Do you synthesize thiophosphate modified random hexamers?
FAQ ID -711
View
Has anyone verified whole genome amplification accuracy with Sequencing?
FAQ ID -701
View
How best to handle the light sensitive LiquiChip reagents?
FAQ ID -735
View
Do you have a protocol for the fixation of cells transfected with fluorescently labeled siRNA?
FAQ ID -793
View
Do you have information about the cleanup of single-stranded DNA (ssDNA) with QIAquick columns?
FAQ ID -759
View
Do you have information on the use of recombinant DNA and RNA as absolute standards for realtime RT-PCR?
FAQ ID -729
View
Do you have a protocol for the isolation of genomic DNA from paraffin-embedded samples on the BioRobot M48?
FAQ ID -1019
View
Is the TurboCapture Microplate Holder Type B compatible with the Biomek FX robotic workstation?
FAQ ID -1040
View
How can I prevent running out of M48 Kit reagents before the full number of samples are processed?
FAQ ID -1042
View
What are the additional plasmid bands I see on my gel?
FAQ ID -1059
View
Is it possible to scale up QuantiTect Reverse Transcription reactions to allow use of larger amounts of RNA?
FAQ ID -1063
View
Is it possible to use the PhosphoProtein Purification Kit in batch format?
FAQ ID -1069
View
What are the recommended storage conditions for the QuantiTect Reverse Transcription Kit and its components?
FAQ ID -1077
View
What is the difference between Buffers RLT and RLT Plus?
FAQ ID -1043
View
What is the difference between TurboCapture Microplate Holders Type A and Type B?
FAQ ID -1038
View
Is there a limit to the size of proteins that can be purified using the Qproteome Glycoprotein Kits?
FAQ ID -1071
View
What blocking reagents do you recommend for chemiluminescent detection using Penta-, Tetra-, and RGS-His antibodies?
FAQ ID -1093
View
Is Factor SB of the HotStar HiFidelity Polymerase Kit the same as Factor MP of the QIAGEN Multiplex PCR Kit?
FAQ ID -1049
View
How can I transfect siRNA into insect cells such as Drosophila melanogaster S2?
FAQ ID -1046
View
How can I tell TurboCapture Microplate Holder Type A from Type B?
FAQ ID -1039
View
How much template can I use in the reaction and what is the maximum volume of template that can be used in the QuantiTect Kits?
FAQ ID -1086
View
I am seeing a precipitate after adding LyseBlue reagent to Buffer P1. What should I do about that?
FAQ ID -1045
View
How much dye-labeled siRNA should I use to monitor transfection efficiency when using HiPerfect Transfection Reagent?
FAQ ID -1058
View
What is the error rate of HotStar HiFidelity DNA Polymerase?
FAQ ID -1048
View
How do I perform an Acetone Precipitation for concentrating and desalting protein samples?
FAQ ID -1035
View
Which kit should I use for RNA isolation from Cartilage?
FAQ ID -1026
View
Which QIAGEN kit do you recommend for purifying plasmid DNA suitable for transfection of sensitive cells?
FAQ ID -1092
View
Where can I find the serial number on QIAGEN's BioRobots?
FAQ ID -1064
View
Which kit can be used for simultaneous purification of DNA and RNA from biopsies?
FAQ ID -1089
View
Why does my PCR product show up later when comparing the QuantiTect SYBR Green PCR Kits with Roche kits using the same annealing temperature?
FAQ ID -1083
View
Why is there RNA in the DNA eluate when using the AllPrep DNA Mini Spin Column?
FAQ ID -1044
View
Will the "classic" RNeasy Mini Kit be discontinued after the launch of the Allprep DNA/RNA Mini Kit?
FAQ ID -1054
View
Why does TA/UA cloning work with your proofreading HotStar HiFidelity DNA Polymerase?
FAQ ID -1053
View
Why do melting temperatures differ between PCR fragments amplified with QIAGEN's QuantiTect SYBR Green PCR Kits and Roche Kits?
FAQ ID -1084
View
Where can I find a protocol for cleanup of already purified plasmid DNA?
FAQ ID -1031
View
What is the protein loading capacity per column of the Qproteome Albumin/IgG Depletion Kit?
FAQ ID -1090
View
What is the largest PCR amplicon that can be amplified with the HotStar HiFidelity Polymerase Kit?
FAQ ID -1047
View
What is the range of melting temperatures for the QuantiTect Primer Assay amplicons in the dissociation curve?
FAQ ID -1079
View
What is the recipe for 1x PBS solution?
FAQ ID -1030
View
What should I use as a standard for absolute quantification in real-time PCR?
FAQ ID -1085
View
What vacuum pressure should be applied using the QIAvac 24 Plus for the QIAamp DSP Virus Kit and Blood Mini Kit Protocols?
FAQ ID -1033
View
What is your recommendation for cleaning the TissueLyser Bead Dispenser (96-well format)?
FAQ ID -1055
View
What is the resin in the Qproteome Murine Albumin and Qproteome Albumin/IgG Depletion Kits?
FAQ ID -1034
View
What is the volume of the eluate when using either spin or vacuum procedures with the QIAprep 96 Turbo Miniprep Kit?
FAQ ID -1067
View
How can I prevent clogging of QIAfilter cartridges?
FAQ ID -1060
View
Do I have to discard Buffer RLT with beta-Mercaptoethanol (ß-ME) added to it after 1 month of storage?
FAQ ID -1037
View
Do I have to use a ramp time of 2°C/sec on the LightCycler when using Quantitect Primer Assays?
FAQ ID -1065
View
Does an activation time of 15 minutes influence the performance of the HotStarTaq Plus DNA Polymerase?
FAQ ID -1050
View
Do I have to use CoralLoad Gel loading dye when using your HotStarTaq Plus DNA Polymerase?
FAQ ID -1052
View
Do you have a protocol for the isolation of RNA from bacterial cultures using RNAprotect Bacteria Reagent and QIAzol Lysis Reagent?
FAQ ID -1020
View
Do you have a protocol for the isolation of DNA from forensic samples using the BioRobot M48?
FAQ ID -1017
View
Can the USB Port of the BioSprint 96 instrument be used to transfer programs from a PC to the instrument?
FAQ ID -1032
View
Do you have a protocol for the isolation of DNA from mouse tails using MagAttract technology on BioRobot M96?
FAQ ID -1007
View
Are your FlexiTube siRNAs preferentially designed to any particular region of the target gene?
FAQ ID -1091
View
Are the CoralLoad dyes in the HotStarTaq Plus PCR Buffer visible when loading small amounts of PCR product onto a gel?
FAQ ID -1051
View
Are the new EZ1 RNA Kits (cat. no. 958034; 959034; 956034) compatible with the previous EZ1 RNA Card (cat. no. 9015590) or EZ1 RNA Universal Tissue Card (cat. no 9016384)?
FAQ ID -1025
View
Can PCR fragments generated with the EasyXpress Linear Template Kit be cloned into the pIX4.0 vector of the EasyXpress Protein Synthesis Insect Kit?
FAQ ID -1078
View
Can the QProteome O-Glycan Glycoprotein Kits be used for blood cells?
FAQ ID -1036
View
Can the Reverse Transcriptases of the QuantiTect Reverse Transcription Kit and the QuantiTect Probe RT-PCR Kit be used interchangeably?
FAQ ID -1066
View
Can the AllPrep RNA/Protein Kit be used with tissue?
FAQ ID -1095
View
Can the Blocking Reagent supplied with the Penta-His HRP Conjugate be used with other antibodies?
FAQ ID -1073
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Do you recommend 1-step or 2-step real-time RT-PCR for gene expression analysis?
FAQ ID -1056
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How can I check for purity of RNA isolated using RNeasy Kits?
FAQ ID -1023
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How can air bubbles in PhosphoProtein Purification Columns be avoided?
FAQ ID -1070
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How can I increase low-copy plasmid DNA yields using QIAfilter Plasmid Kits?
FAQ ID -1062
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How can I increase DNA concentration using QIAprecipitators of the HiSpeed Plasmid Kits?
FAQ ID -1061
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How can I check the integrity of RNA purified using RNeasy Kits?
FAQ ID -1024
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How can I ensure complete genomic DNA removal when using the RNase-Free DNase Set?
FAQ ID -1087
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Do you have information on the reproducibility of DNA yields from blood using the M48 BioRobot?
FAQ ID - 1004
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Do you have a protocol for transient transfection of HeLa-S3 cells in 60 mm dishes using PolyFect?
FAQ ID -1012
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Do you have a protocol for transient transfection of HEK 293 cells in 75 cm3 flasks?
FAQ ID -1014
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Do you have a protocol for transient transfection of cells in 96-well plates using PolyFect Transfection Reagent?
FAQ ID -1013
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Do you have a protocol for the isolation of total nucleic acids from cells on the M48 BioRobot?
FAQ ID -1009
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Do you have a protocol for the isolation of total nucleic acids from tissue on the M48 BioRobot?
FAQ ID -1008
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Do you have a protocol for vacuum processing of blood samples with the QIAamp DNA Blood Midi or Maxi Kits?
FAQ ID -1011
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Do you have information on realtime RT-PCR performance of RNA isolated from monocytes on the M48 BioRobot?
FAQ ID -1010
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Do you have information on PCR template preparation from preserved blood samples on the M48 BioRobot?
FAQ ID - 1003
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