PCR/qPCR/dPCR
满怀信心地进行特异性、灵敏且稳健的 PCR
在进行基因扩增时，您的实验成功与否取决于几个关键因素，其中包括通量、检测灵敏度、反应的稳健性（即使使用困难模板）以及可靠的数据均一化。您可能还需要克服与您正在使用的 PCR 方法相关的特定挑战。
无论您选择哪种方法，我们都能为终点法 PCR、qPCR 和 dPCR 提供丰富的优化解决方案。我们的试剂盒、assay、仪器和附件可帮助您在几乎任何应用种都能更有信心地扩增和检测靶标以及简化您的工作流程。
- Measure gene expression (mRNA, miRNA)
- Quantify DNA or RNA targets
- Detect & quantify pathogen and/or viral RNA/DNA
- Quantify residual DNA
- Convert RNA to cDNA for quantification
- Mutation detection (including rare/ultra-rare variants)
- Analyze copy number variation (CNV)
- Detect viral load
- Gene expression quantification
- Quantify low-abundance targets
- Measure residual DNA
- Amplify DNA for cloning or sequencing
- Qualitative pathogen detection (presence/absence)
- CRISPR screening
- Genotyping
- Convert RNA to cDNA for storage
- Cq/Ct values
- Relative quantification (ΔΔCq) or absolute quantification from standards
- cDNA for downstream use
- Absolute target quantification (copies/µL reaction)
- Fractional abundance (%)
- Mutation/target frequency
- Copy number per genome
- Amplified DNA or cDNA product
- Band visualization on gel
- Presence/absence result
- Isolated DNA or cDNA
- Isolated RNA/mRNA/miRNA
- Some kits support crude samples such as cells, blood, dried blood spots, saliva, buccal swabs, or gargle
- Isolated DNA or cDNA
- Isolated RNA/mRNA/miRNA
- Purified samples recommended
- Isolated DNA or cDNA
- Isolated RNA
- Quantitative real-time PCR
- Reverse transcription
- Amplification and fluorescence-based detection in real-time for quantification
- Quantitative digital PCR
- Partitions the sample into thousands of reactions and uses end-point PCR + Poisson statistics for absolute quantification without a standard curve
- Standard end-point PCR
- DNA amplification and visualization (e.g., gel-based) and downstream applications
- When reliable quantification is required over a wide linear range
- When standard curve-based or relative quantification is sufficient
- When precise quantification is required
- When detecting rare alleles or small fold changes
- When standard curves are undesirable
- When working near the detection limit (low-abundance targets)
- When qualitative detection without quantification is sufficient
- When preparing amplicons for cloning, genotyping, sequencing, or other downstream applications
- Real-time PCR thermal cycler
- Dye-based (e.g., SYBR Green) or probe-based detection
- QIAcuity dPCR platform
- Probe‑based or intercalating dye detection
- Thermal cycler
- Visualization (e.g., via agarose gel electrophoresis)
浏览 PCR/qPCR/dPCR
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找到 156 个产品
QIAcuity High Multiplex Probe PCR Kit
For challenging samples and dPCR multiplexing beyond five targets
QIAprep& Plasmodium Assays
For distinguishing between Plasmodium falciparum and non-falciparum species, as well as differentiating four additional species
QIAprep& Plasmodium Kit
For direct qPCR detection of Plasmodium spp. from dried blood spots or whole blood.
QIAcuity RCL Quant Kit
For the detection of Replication Competent Lentivirus (RCL) with high accuracy using the QIAcuity digital PCR instruments
ID SOLUTIONS IDNAPTEX dPCR Assays
For detecting genomic alterations, such as point mutations, deletions and fusions in DNA extracted from liquid and tissue biopsies using multiplex digital PCR for cancer research applications
dPCR RNA-Plex Assays
For accurate and reliable probe-based gene expression analysis of mRNA and lncRNA targets in multiplex dPCR workflows.
QIAGEN OneStep RT-PCR Kit
用于灵敏、特异性的一步法RT-PCR
QIAGEN Multiplex PCR Kit
用于高特异性和灵敏度的多重PCR，无需优化
Rotor-Gene Q (CA)
For outstanding performance in real-time PCR
QuantiTect Reverse Transcription Kit
快速合成cDNA，进行两步法real-time RT-PCR，用于基因表达分析
HotStarTaq DNA Polymerase
高特异性扩增，所需优化次数少
RT2 Profiler PCR Arrays
用于可靠、灵敏的基因表达谱分析
REPLI-g Single Cell Kit
对单个细胞或有限的样本进行高度均一的全基因组扩增（WGA）
QuantiTect RT-PCR Kit
用于使用 SYBR® Green I 进行一步法 qRT-PCR 以及使用序列特异性探针进行基因表达分析的一步法 qRT-PCR 和多重 qRT-PCR
RT2 SYBR® Green qPCR Mastermixes
用于real-time PCR分析
HotStarTaq Master Mix Kit
高特异性扩增，用于多种PCR分析
QuantiTect PCR Kit
适用于使用 SYBR Green I 的 qPCR 和两步法 qRT-PCR，qPCR 和两步法 qRT-PCR，以及使用序列特异性探针的多重 qPCR 和两步法多重 qRT-PCR
Rotor-Gene Q
表现卓越的定量PCR
QuantiFast Pathogen +IC Kit
用于灵敏而又可靠地检测病毒 RNA/DNA 和细菌 DNA，包括内质控
HotStarTaq Plus DNA Polymerase
快速、高特异性扩增，适合多种应用
miRCURY LNA SYBR® Green PCR Kits
For high-performance PCR with the miRCURY LNA miRNA PCR System
Taq DNA Polymerase
用于常规和特殊的PCR
QuantiTect Primer Assays
使用SYBR® Green进行两步法和一步法定量RT-PCR，用于基因表达分析
miRCURY LNA miRNA PCR Assay
使用基于 LNA 优化、SYBR ® Green 的 miRNA PCR系统，实现极其灵敏和特异的 miRNA 定量
miRCURY PCR Controls
Validated primer sets for normalization of miRNA expression levels in real-time PCR analyses
RT2 First Strand Kit
用于更快、更高效地合成cDNA，并去除RNA样本中的基因组DNA
miRCURY LNA RT Kit
使用 SYBR Green 或水解探针与 miRCURY LNA miRNA PCR 系统进行最佳的第一链 cDNA 合成
HotStarTaq Plus Master Mix Kit
快速、高特异性扩增，适合多种应用
Custom Microbial DNA qPCR Arrays
real-time PCR方法的微生物鉴定或分析
QuantiTect Virus Kit
适用于高灵敏度检测病毒 RNA 或 DNA
RT2 qPCR Primer Assays
准确、可靠的基因表达分析
Type-it Microsatellite PCR Kit
快速可靠的微卫星位点多重PCR分析
QuantiNova PCR Kit
适用于使用基于 SYBR Green 的 qPCR 进行高灵敏度、特异性和超快探针法 Real-time PCR 和多重 PCR 以及获得无与伦比的结果
QIAGEN Multiplex PCR Plus Kit
用于成功的进行多重PCR反应
Omniscript RT Kit
在终点法PCR中，用于RNA模板量为50 ng到2 μg的逆转录反应
Custom RT2 Profiler PCR Arrays
用于可靠、灵敏的基因表达谱分析
Microbial DNA qPCR Assays
采用real-time PCR技术进行微生物检测
mericon Sample Preparation and Bacteria Kits
For extraction of high-quality DNA from microbial enrichment cultures and raw or processed foods
QuantiNova Reverse Transcription Kit
用于快速的cDNA合成和高度可重复性的两步法RT-PCR
QuantiNova Pathogen +IC Kit
用于超快速地同时检测病毒 RNA/DNA 和细菌 DNA，包含内质控
miRCURY LNA miRNA Custom PCR Panel
基于 LNA 增强 SYBR® Green 的 miRNA qPCR 分析的定制 96 孔板和 384 孔板
Taq PCR Master Mix Kit
预混合的PCR反应液方便PCR体系构建
REPLI-G Kits
For highly uniform whole genome amplification from 10ng of genomic DNA
QuantiNova RT-PCR Kit
适用于使用序列特异性探针进行高灵敏度和特异性的一步法 qRT-PCR 和多重 qRT-PCR，或使用 SYBR® Green I 进行基因表达分析的一步法 qPCR
QuantiFast SYBR® Green RT-PCR Kit
使用SYBR Green I的快速一步法定量RT-PCR，适用于基因表达分析
Taq PCR Core Kit
用于常规和特殊的PCR，包含dNTP混合液
miRCURY LNA miRNA Focus PCR Panels
For exceptionally sensitive and specific miRNA profiling using LNA-enhanced, SYBR® Green-based miRNA qPCR
FastLane Cell RT-PCR Kits
For SYBR® Green real-time one-step RT-PCR analysis and probe-based real-time one-step RT-PCR and multiplex RT-PCR analysis from cultured cells without RNA purification
REPLI-g Advanced DNA Single Cell Kit
For whole genome amplification (WGA) from single eukaryotic cells, limited samples or purified genomic DNA
miRCURY LNA miRNA miRNome PCR Panels
For exceptionally sensitive and specific miRNA profiling using LNA-enhanced, SYBR® Green-based miRNA qPCR
QIAGEN Fast Cycling PCR Kit
用于超快速和特异性PCR，适合各种PCR仪
Sensiscript RT Kit
每次反应可逆转录少于50 ng的RNA，用于终点法PCR
REPLI-g WTA Single Cell Kit
从单细胞起始的总RNA或mRNA全转录本扩增
miScript miRNA Mimic
适合利用合成 miRNA 研究 miRNA 功能和基因调控
Microbial DNA qPCR Arrays
real-time PCR方法的微生物鉴定或分析
qBiomarker Somatic Mutation PCR Arrays
用于快速、准确地分析体细胞DNA的突变状态
QIAGEN OneStep Ahead RT-PCR Kit
适用于快速一步法RT-PCR，灵敏度高、特异性强、保真性出色
QuantiTect Whole Transcriptome Kit
对珍贵的RNA样本进行无限次real-time PCR分析
qBiomarker Somatic Mutation PCR Assays
用于检测癌症和肿瘤发生时出现的特定DNA序列突变
REPLI-g Cell WGA & WTA Kit
从细胞和有限的样本中同时扩增全基因组和全转录组
AllTaq Master Mix and PCR Core Kits
For ultrafast and versatile hot-start PCR in all applications
Microbial DNA qPCR Assay Kits
采用real-time PCR技术进行微生物检测
miRCURY LNA miRNA Custom PCR Assays
For SYBR® Green qPCR-based miRNA quantification using LNA-optimized miRNA PCR primer sets
miRCURY LNA miRNA PCR Starter Kit
使用基于 SYBR® Green 的 qPCR 开始 miRNA 定量实验
RT2 SYBR® Green FAST Mastermixes
用于real-time PCR分析
RT2 PreAMP cDNA Synthesis Kit
从1–100 ng RNA中预扩增cDNA，用于RT2 Profiler PCR Arrays分析
FastLane Cell cDNA Kit
快速制备用于定量RT-PCR的cDNA，无需RNA纯化
UCP Multiplex PCR Kit
Ultra-Clean Production master mix for multiplex hot-start PCR and microbiome applications
REPLI-g FFPE Kit
直接对福尔马林固定、石蜡包埋组织（FFPE）中的DNA进行全基因组扩增
qBiomarker Copy Number PCR Assays
用于分析特定位点的拷贝数变化和改变
RT2 lncRNA PCR Arrays
通过经湿法验证的qPCR检测引物对与信号转导及疾病机制相关的lncRNA的表达进行分析
REPLI-g Mitochondrial DNA Kit
对人类线粒体DNA进行高度均一的全基因组扩增
QIAGEN PCR Cloning Kits
For direct cloning of PCR products generated by Taq DNA polymerases
RNA Spike-In Kit, For RT
For quality control of the RNA isolation and cDNA synthesis steps of miRCURY LNA miRNA PCR experiments
Type-it Mutation Detect PCR Kit
用于对突变进行准确、可靠的多重PCR分析
Certal Residual DNA Detection Kit
适用于灵敏的探针法 CHO 宿主细胞残留 DNA（包含内质控）检测
RT2 HT First Strand Kit
用于从96个RNA样本中快速、高效地合成cDNA，并去除基因组DNA
Custom qBiomarker Somatic Mutation PCR Arrays
用于快速、准确地分析自定义的一组基因的体细胞DNA突变状态
miRCURY LNA miRNA QC PCR Panel
用于在基于 SYBR® Green 的 qPCR 分析之前快速、简便地对 miRNA 样品进行质量控制
RT2 lncRNA qPCR Assay
用于通过实时定量PCR分析长链非编码基因的表达
XpressRef Universal Total RNA
作为基因表达检测方法的阳性对照
QIAsymphony mericon Bacteria Kit
从细菌富集培养液中自动分离病原体DNA
UCP HiFidelity PCR Kit
Ultra-Clean Production master mix for high-fidelity hot-start PCR and microbiome applications
RT2 lncRNA PCR Array Modification
可通过经局部修饰的现有信号转导或疾病相关PCR Array分析相关lncRNA表达谱
RT2 PreAMP Pathway Primer Mixes
用于RT² Profiler PCR Arrays分析之前扩增cDNA模板
RT2 Easy First Strand Kit
用于快速、高效地合成cDNA的第一条链，并去除RNA样本中的基因组DNA
Microbial DNA qPCR Multi-Assay Kits
用于real-time PCR方法的微生物鉴定或分析
Rotor-Gene SYBR® Green PCR Demo Kit
用于评估Rotor-Gene Q实时荧光定量PCR分析仪的性能
Microbial DNA Positive Control
作为阳性对照，配合Microbial DNA qPCR Assays使用
mericon Pathogen Detection Assay
For real-time PCR detection of pathogen DNA in food or animal feed
qBiomarker SYBR® Green Mastermixes
用于real-time PCR分析
Custom RT2 PreAMP Pathway Primer Mixes
用于Custom RT² Profiler PCR Arrays分析之前扩增cDNA模板
RT2 RNA QC PCR Arrays
用于在PCR芯片分析前对RNA样本进行质量评估
RT2 qPCR Primer gDNA Controls
检测RNA和cDNA中的基因组DNA污染
Supplemental Microbial qPCR Mastermix (ROX)
用于real-time PCR
RT2 lncRNA PreAMP Primer Mixes
用于实时定量PCR前的cDNA合成
mericon MeatTracker Kit
For detection of animal DNA in food, animal feed or pharmaceutical samples using real-time PCR
UCP PCR Kits
For SYBR Green-based and probe-based, real-time quantitative PCR for microbiome or quality control applications using reagents from Ultra Clean Production
Supplemental Microbial qPCR Mastermix (Fluorescein)
用于real-time PCR
管家基因
用于对相对基因表达图谱分析实验进行归一化处理
miRCURY LNA miRNA Custom Bulk Plate
Customer-configurable SYBR® Green-based miRNA qPCR Assays in convenient plate format
miRCURY LNA miRNA Custom Probe PCR Assay
利用 LNA 增强的、基于探针的 qPCR 技术，对成熟 miRNA 进行极其灵敏和准确的定量分析
miRCURY LNA miRNA Probe PCR Assay
利用 LNA 增强的、基于探针的 qPCR 技术，对成熟 miRNA 进行极其灵敏和准确的定量分析
miRCURY LNA Probe PCR Kit
使用 miRCURY LNA miRNA Probe PCR 系统进行高性能 PCR 分析
qBiomarker Copy Number PCR Arrays
用于分析拷贝数变化和改变
QIAcuity Probe PCR Kit
供与 QIAcuity 数字 PCR 仪器一起使用
QIAcuity UCP Probe PCR Kit
用于 QIAcuity 数字 PCR 仪器的 Ultra Clean Production 试剂盒
QIAcuity OneStep Advanced Probe Kit
适用于在 QIAcuity 数字 PCR 仪器上进行一步法RT-PCR